Phage m13. Medical search. Frequent questions

We compare the results derived from the use of the phage display library with results obtained from analysis of eluted peptides and peptide-binding studies. (ox.ac.uk)

Bacteriophage M13 does not damage the chicken gut. (springeropen.com)
The bacteriophage M13 is a filamentous phage that displays peptides linked to the minor coat protein III gene. (springeropen.com)

The new system, called phage-assisted continuous evolution, or PACE, is based on the continuous culture and selection of the M13 filamentous bacteriophage commonly used in phage display. (genengnews.com)
This thesis explores both sides of the dichotomy in an effort to reveal the mechanics of two biological systems - M13 filamentous bacteriophage and kinesin motor protein - and the properties of their underlying parts. (mit.edu)

Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). (wikipedia.org)
Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
The most common bacteriophages used in phage display are M13 and fd filamentous phage, though T4, T7, and λ phage have also been used. (wikipedia.org)
Now, an international team led by researchers at Imperial College London has had promising results using specialised viruses called bacteriophages or 'phages' to target cancer cells in the brains of mice. (imperial.ac.uk)
At the heart of the approach are bacteriophages, or 'phages' - specialised types of virus that are perfectly evolved to target bacteria. (imperial.ac.uk)

To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. (lu.se)
Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. (lu.se)
The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. (lu.se)
We inoculated phage M13 or E. coli ER2738 (ECR) infected with M13 into birds at different ages. (springeropen.com)
Phage M13 remains viable in the bird's intestine if inoculated with E. coli ER2738. (springeropen.com)
The phage gene and insert DNA hybrid is then inserted (a process known as "transduction") into E. coli bacterial cells such as TG1, SS320, ER2738, or XL1-Blue E. coli. (wikipedia.org)
If a "phagemid" vector is used (a simplified display construct vector) phage particles will not be released from the E. coli cells until they are infected with helper phage, which enables packaging of the phage DNA and assembly of the mature virions with the relevant protein fragment as part of their outer coat on either the minor (pIII) or major (pVIII) coat protein. (wikipedia.org)
PACE essentially comprises a system by which E. coli cells continuously flow through a fixed-volume vessel, or lagoon, which contains a replicating population of phage DNA vectors (the selection phage) encoding the gene or genes of interest. (genengnews.com)
Evolution of parasitism and mutualism between filamentous phage M13 and Escherichia coli . (weebly.com)

In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. (wikipedia.org)
In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
Giving the animals doses of TMZ then amplified the process, boosting the effects of the gene therapy delivered by the phages and helping to reduce the size of the tumours. (imperial.ac.uk)
These precise epitope investigations were performed using two different phage-displayed random peptide libraries with different characteristics (T7 phage library gene X C9C and linear 9-mers, and M13 filamentous phage library gene III C7C and linear 12-mers and gene VIII 5C4C4). (nottingham.ac.uk)
To further define the N-terminal MoAb epitope, sequencing of N-terminal MoAbs reactive peptides which were obtained from the successful biopanning using M13 gene VIII 5C4C4 phage Iibrary and were selected by high affinity binding in immnuo-blotting assay using nitro-cellulose membrane and in capture ELISA. (nottingham.ac.uk)
Thus, the overall motif or GAD-6 may correspond to 523-528 aa of GAD-65 (M-S-R-L-S-K). To further define the GAD-6 epitope, sequencing or GAD-6 reactive peptides which were obtained from the successful biopanning using MI3 gene III C7C and linear 12-mers phage libraries, did not show a clear motif and did not show reactivity with GAD-6 by capture ELISA. (nottingham.ac.uk)
Typically, nucleotide sequences encoding the protein/peptide library are fused to a gene encoding a phage coat protein thus allowing them to be displayed on the phage exterior. (cdc.gov)
In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. (abcam.co.jp)
The system achieves continuous selection by linking the desired activity to the production of infectious progeny phage containing the evolving gene(s). (genengnews.com)
We have created a strong promoter and translation initiation control element to regulate the expression of capsid gene of T7 phage. (alphalifetech.com)
Rephine.r: a pipeline for correcting gene calls and clusters to improve phage pangenomes and phylogenies. (weebly.com)

The use of phage display libraries exploits the ability of MHC class II molecules to exchange peptides in solution and thus select out peptide sequences with high-affinity binding from a large array of random peptides. (ox.ac.uk)
In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. (wikipedia.org)
Recombinant Human Novel Coronavirus Spike glycoprotein(S) (D614G), partial (Active) referenced in "Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries", Cell discovery , 2021. (cusabio.com)
A possible explanation for this is that the peptides which are specific to GAD-6 are not present in these M13 pIII phage libraries. (nottingham.ac.uk)
Further, peptide libraries based on phage T7 are reported to exhibit fewer amino acid biases and increased peptide diversity over M13 libraries in some cases. (alphalifetech.com)
M13 is a filamentous virus that has elicited the interest of the engineering community for its use in phage display of combinatorial peptide libraries and more recently as a 1D template for organizing and growing inorganic materials. (mit.edu)

We have analysed the peptide binding motifs of HLA-DRB1*1301 and *1302 using affinity purified HLA-DR13 molecules to purify sequentially HLA-DR13-binding peptides from a large random library of M13 phage containing nonamer inserts in the pIII coat protein. (ox.ac.uk)
M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. (nih.gov)

Tissue, cells or virus corresponding to M13 + Fd Bacteriophage Coat Proteins. (abcam.co.jp)
In contrast to the common forms of M13, in which the presented peptides of interest are affixed through their carboxyl terminus, T7 phage peptides are anchored to viral coat proteins through their amino ends (N-terminus). (alphalifetech.com)

The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. (lu.se)
the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)
The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those of other molecules. (wikipedia.org)
Phage display technology was further developed and improved by groups at the Laboratory of Molecular Biology with Greg Winter and John McCafferty, The Scripps Research Institute with Richard Lerner and Carlos Barbas and the German Cancer Research Center with Frank Breitling and Stefan Dübel for display of proteins such as antibodies for therapeutic protein engineering. (wikipedia.org)
Phage eluted in the final step can be used to infect a suitable bacterial host, from which the phagemids can be collected and the relevant DNA sequence excised and sequenced to identify the relevant, interacting proteins or protein fragments. (wikipedia.org)
To elucidate the ligand-binding surface of the CC chemokine-binding proteins Evasin-1 and Evasin-4, produced by the tick Rhipicephalus sanguineus, we sought to identify the key determinants responsible for their different chemokine selectivities by expressing Evasin mutants using phage display. (unige.ch)
The results also suggest that phage display may offer a useful approach for rapid investigation of the pharmacophores of small inhibitory binding proteins. (unige.ch)
The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)
Early studies on nonsense mutants of phage phi6 suggested that the major envelope protein P9 and the non-structural protein P12 are the only proteins needed for phi6 virion envelopment [ 23 ]. (biomedcentral.com)
Unlike the filamentous systems, T7-displayed peptides or proteins do not require secretion through the bacterial cell membrane, and thus are not as biologically constrained as the M13 phage display system. (alphalifetech.com)
Anyway, the T7 phage display system is suitable for high-copy display of peptides or proteins with small molecule weight (50 amino residues or shorter). (alphalifetech.com)
Recent studies indicate that M13 bacteriophage, a very large nanoparticle, binds to β-amyloid and α-synuclein proteins, leading to plaque disaggregation in models of Alzheimer and Parkinson disease. (thejns.org)

Both applications rely on the direct link between genotype and phenotype, whereby each phage particle displays fusion molecules on its proteinaceous capsid and simultaneously carries the encoding DNA. (mit.edu)

By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein that binds to one of those targets on its surface will remain while others are removed by washing. (wikipedia.org)

Conditional lethal mutants of the small filamentous coliphage M13. (wikidata.org)

They used a genetically modified virus called an M13 phage to target tumours and deliver a killer payload to the cancer cells. (imperial.ac.uk)

The mutants were displayed on M13 phage particles, and binding to chemokine was assessed by ELISA. (unige.ch)

It is a filamentous phage consisting of single-stranded DNA and is circularly permuted. (nih.gov)

Single-molecule assays centered around the "zinc fingered-phages" are being developed to establish M13 as a standalone, single-molecule building block for achieving universal connectivity and for unlocking future studies on the nature of protein-DNA interactions. (mit.edu)

Smith and Winter were awarded a half share of the 2018 Nobel Prize in chemistry for their contribution to developing phage display. (wikipedia.org)

May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages. (abcam.co.jp)

Moreover, the speed of the phage lifecycle means that progeny phage production starts about 10 minutes after infection of the bacteria, so PACE can effectively mediate many generations of selective phage replication much faster than other laboratory evolution approaches. (genengnews.com)
Bacteriophage T7 is a lytic phage, replicating and amplifying within the host cells, and phage progeny is released by host cell lysis. (alphalifetech.com)

Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
Phages are viruses that specifically infect bacteria. (ukdiss.com)

Most of the phages are DNA viruses that code for several genes responsible for virus replication. (microbenotes.com)

We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. (nih.gov)
We have developed NP binding antibodies to commercially important NPs including QDs and TiO2 NPs using phage display technology. (cdc.gov)

de Kruif J,, Boel E, Logtenberg T. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions . (genomicglossaries.com)
Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. (abcam.co.jp)

Those that remain can be eluted, used to produce more phage (by bacterial infection with helper phage) and to produce a phage mixture that is enriched with relevant (i.e. binding) phage. (wikipedia.org)
citation needed] The use of a helper phage can be eliminated by using 'bacterial packaging cell line' technology. (wikipedia.org)

Pseudomonas phage phi6 infects Gram-negative plant-pathogenic Pseudomonas species [ 7 , 8 ] and is the type member of this family [ 9 ]. (biomedcentral.com)

The phages were modified to target receptors found exclusively on the surface of cancer cells. (imperial.ac.uk)

As a result, a large amount of capsid/fusion protein is produced during infection, leading to the display of 415 copies of peptide on the surface of the phage. (alphalifetech.com)

Analysis of the phage bound to these DR molecules suggests binding motifs. (ox.ac.uk)
Phage display (PD) is a tool for developing new molecules to control pathogens. (springeropen.com)

The goal is to synthesize nanoparticles bound with phages that can be targeted towards breast cancer therapy and diagnostics. (ukdiss.com)

Main producer of zinc sulfate is a filamentous phage, the first two are displayed as generic. (ovh.net)
As such, M13 was further engineered to incorporate variants of the zinc finger, DNA-binding domain of transcription factor Zif268 as minor coat fusions. (mit.edu)

Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)

Analysis of peptide-binding motifs for two disease associated HLA-DR13 alleles using an M13 phage display library. (ox.ac.uk)
Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
Alpha Lifetech Inc. has developed a T7 phage display technology, by which we can offer a convenient tool for peptide library construction and screening. (alphalifetech.com)

Recently, we identified a linear 12-AA M13 phage pill sequence, NH-12-5-2 (DYFSSPYYEQLF), that binds with high affinity to SWNHs surfaces. (nyu.edu)

Therefore, the M13 pVIlI 5C4C4 worked with N-terminal MoAb by expressing a relevant sequence for the N-terminal MoAb but which is unlike its epitope in GAD-65. (nottingham.ac.uk)
Using M13 phage display technology, polypeptides directed again SWNHs surfaces have been created for a number of nanotechnology and pharmaceutical purposes, yet the molecular mechanism of polypeptide sequence interaction and binding to SWNHs surfaces is not known. (nyu.edu)
These nonbonding interactions may play a role in the recognition and binding of this phage sequence region to SWNHs surfaces. (nyu.edu)

This study evaluated whether phage M13 can remain viable in the chicken gastrointestinal tract and whether it causes injury or humoral immune response. (springeropen.com)
Being a larger structure than the purified peptide, phage M13 displaying selected peptides may stimulate the local immune response and/or bind to microorganisms more successfully. (springeropen.com)

Peptides selected by PD are commonly synthesised and tested, but the use of phage M13 displaying the selected peptides as a direct biding in the intestinal tract has not yet been tested. (springeropen.com)

Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. (wikipedia.org)

The workhorse of phage display is the M13 bacteriophage virus. (genomicglossaries.com)

To determine the feasibility, safety, and characteristics of convection-enhanced delivery (CED) of M13 bacteriophage to the brain, the authors perfused primate brains with bacteriophage. (thejns.org)

The phage-infected cells demonstrated impaired function of the oxidative and the glutamate-dependent acid resistance systems. (lu.se)
Despite the threat to bacteria, phages are unable to infect animal cells and so are harmless to humans. (imperial.ac.uk)

Scientists claim to have developed a new phage-based approach to biomolecular evolution that is about 100 times faster than conventional laboratory evolution techniques and far less labor intensive. (genengnews.com)

Thus, global transcriptional analysis and functional analysis revealed previously unknown host responses to filamentous phage infection. (lu.se)

After the selection by PD, the peptides displayed by phage M13, are sequenced, characterised and synthesised for later use. (springeropen.com)
This means that mutations only accumulate in the evolving selection phage population, the authors note. (genengnews.com)
The lytic feature of T7 phage also shortens the time separating one round of biopanning selection from the next. (alphalifetech.com)

Anatomy6

Organisms51

Diseases6

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes89

Disciplines and Occupations4

Technology, Industry, Agriculture3

Information Science4

Health Care7

Eluted peptides1

Bacteriophage M132

Filamentous bacteriophage2

Bacteriophages5

Coli9

Gene12

Libraries6

PIII2

Coat Proteins2

Proteins12

Genotype and pheno1

Microtiter plate1

Coliphage1

Genetically1

Particles1

Single-stranded1

Assays1

20181

ELISA1

Progeny2

Infect bacteria2

Viruses1

Antibodies2

Antibody2

Bacterial2

Infects1

Receptors1

Capsid1

Molecules2

Bound1

Zinc2

Vectors1

Library3

High-affinity1

Sequence3

Immune2

Commonly1

Protein interactions1

Virus1

Characteristics1

Cells2

Laboratory1

Functional1

Selection3