• They cocultured different strains of Y. pestis with amoebae, killed any extracellular bacteria, lysed the amoebae, and finally cultured this lysate. (labroots.com)
  • The researchers knew that the protein PhoP helps Y. pestis survive inside of macrophages, so they tested whether the same protein helped the bacteria survive inside of an amoeba. (labroots.com)
  • Y. pestis are gram-negative bacteria from Enterobacteriaceae family, they have ovoid shape with bipolar staining (more intensively stained at poles). (sciencephoto.com)
  • Pesticinogenic and Ca2+-dependent strains of Yersinia pestis harbored plasmids of about 6 and 45 megadaltons, respectively. (asm.org)
  • They found that some strains of Y. pestis were able to survive inside of the amoebae for over 5 days! (labroots.com)
  • Present-day Y. pestis strains, although all similarly pathogenic, can be classified into three biovars [Antiqua (A), Medievalis (M), and Orientalis (O)] on the basis of their ability to use glycerol and to reduce nitrate. (pnas.org)
  • Studies characterizing virulence determinants of Y. pestis have identified novel mechanisms for overcoming host defenses. (asm.org)
  • Straley SC, Harmon PA (1984) Growth in mouse peritoneal macro- phages of Yersinia pestis lacking established virulence determinants. (springer.com)
  • Zhang Y, Gao H, Wang L, Xiao X, Tan Y, Guo Z, Zhou D, Yang R (2011) Molecular characterization of transcriptional regulation of rovA by PhoP and RovA in Yersinia pestis . (springer.com)
  • Extensive insertion sequence-mediated genome rearrangements and reductive evolution through massive gene loss, resulting in elimination and modification of preexisting gene expression pathways, appear to be more important than acquisition of genes in the evolution of Y. pestis . (pnas.org)
  • Together, these plasmids, and a pathogenicity island called HPI, encode several proteins that cause the pathogenesis, for which Y. pestis is famous. (wikipedia.org)
  • Hinnebusch BJ, Fischer ER, Schwan TG (1998) Evaluation of the role of the Yersinia pestis plasminogen activator and other plasmid-encoded factors in temperature-dependent blockage of the flea. (springer.com)
  • Last but not least, because Y. pestis is able survive inside of human macrophages, and human macrophages are pretty similar to amoebae, researchers at Washington State University set out to see if Y. pestis could survive inside of amoebae. (labroots.com)
  • Thus, the study of the interaction between Y. pestis and macrophages will increase our understanding of how Yersinia subverts the host immune response. (springer.com)
  • Bi Y, Du Z, Han Y, Guo Z, Tan Y, Zhu Z, Yang R (2009) Yersinia pestis and host macrophages: immunodeficiency of mouse macrophages induced by YscW. (springer.com)
  • Bi Y, Du Z, Yang H, Guo Z, Tan Y, Zhu Z, Yang R (2009) Reduced apoptosis of mouse macrophages induced by yscW mutant of Yersinia pestis results from the reduced secretion of YopJ and relates to caspase-3 signal pathway. (springer.com)
  • Gao H, Zhang Y, Yang L, Liu X, Guo Z, Tan Y, Han Y, Huang X, Zhou D, Yang R (2011) Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis . (springer.com)
  • wherein the Yersinia pestis protein comprises full-length F1 protein or full-length LcrV protein. (freepatentsonline.com)
  • 13. The vaccine composition of claim 11, wherein the Yersinia pestis protein comprises at least two sequences selected from the group consisting of SEQ ID NOs. (freepatentsonline.com)
  • 14. The vaccine composition of claim 13, wherein the Yersinia pestis protein comprises full-length F1 protein and full-length LcrV protein. (freepatentsonline.com)
  • 15. The vaccine composition of claim 13, wherein the Yersinia pestis protein comprises a plurality of variants of F1 protein. (freepatentsonline.com)
  • Here we show that YopM prevents Y. pestis induced activation of the Pyrin inflammasome induced by the RhoA-inhibiting effector YopE, which is a GTPase activating protein. (bibsys.no)
  • Many novel sRNAs were identified from Y. pestis grown in vitro and in the infected lungs of mice suggesting they play role in bacterial physiology or pathogenesis. (wikipedia.org)
  • Chu MC, Dong XQ, Zhou X, Garon CF. A cryptic 19-kilobase plasmid associated with U.S. isolates of Yersinia pestis: a dimer of the 9.5-kilobase plasmid. (atcc.org)
  • The formation of a biofilm, which often grows as an attached extracellular matrix and blocks the flea proventriculus, enhances flea-borne transmission of Yersinia pestis during flea biting. (springer.com)
  • Vadyvaloo V, Jarrett C, Sturdevant DE, Sebbane F, Hinnebusch BJ (2010) Transit through the flea vector induces a pretransmission innate immunity resistance phenotype in Yersinia pestis . (springer.com)
  • Rempe KA, Hinz AK, Vadyvaloo V (2012) Hfq regulates biofilm gut blockage that facilitates flea-borne transmission of Yersinia pestis . (springer.com)
  • The enteropathogenic yersiniae must survive in soil and water and then bypass the host gastrointestinal mucosa following ingestion, whereas Y. pestis remains within the closed and protected environment of its flea vector, thereby ensuring transmission by intradermal injection, a route that requires extensive dissemination to achieve favored visceral niches which support the bulk of replication in vivo ( 5 , 20 ). (asm.org)