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  • substrate
  • Pertussis-toxin-catalysed [32P]ADP-ribosylation strongly relies on the substrate quality of the alpha-subunits and is influenced by the concentration of nucleotides, beta gamma-subunits, the physicochemical properties of the membranes influencing the availability of Gi alpha for pertussis toxin, and covalent modification of Gi alpha. (biochemj.org)
  • Membrane
  • Membrane localization of the pertussis toxin-sensitive G-protein subunits alpha i-2 and alpha i-3 and expression of a metallothionein-alpha i-2 fusion gene in LLC-PK1 cells. (pnas.org)
  • However, no difference was observed in membrane protein ADP ribosylation when cells were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after pretreatment or continuous treatment with pertussis toxin. (diabetesjournals.org)
  • inhibition
  • Pretreatment with pertussis toxin partly prevented PGE 2 inhibition to 80 ± 4% of controls for first phase and 79 ± 4% of controls for second phase. (diabetesjournals.org)
  • To evaluate whether the partial prevention of PGE 2 inhibition seen with pertussis toxin pretreatment was caused by G 1 heterotrimer association between the preincubation period and the end of perifusion, PGE 2 actions were also examined during continuous treatment with pertussis toxin. (diabetesjournals.org)
  • O2- formation induced by arachidonic acid and gamma-hexachlorocyclohexane were more sensitive to inhibition by pertussis toxin than O2- formation induced by phorbol 12-myristate 13-acetate. (uni-regensburg.de)
  • Membranes prepared from cultured human monocytes contain a 41,000-dalton protein that is ADP-ribosylated by pertussis toxin and may be the inhibitory guanine nucleotide regulatory protein (Ni) mediating this inhibition. (umassmed.edu)
  • vaccination
  • A considerable proportion of our study population with a positive history of childhood vaccination for pertussis was not serologically immune to pertussis. (biomedcentral.com)
  • The incidence of adult pertussis has been estimated to be 200 to 500 per 100,000 person-years, even in highly immunised populations [ 2 - 4 ], which is thought to reflect waning of the immunity from childhood vaccination. (biomedcentral.com)
  • Pathways
  • In the human erythroleukemia cell line, HEL, prostaglandin E2 (PGE2) and the stable prostacyclin analogue, iloprost, increase cytosolic Ca2+ concentration ([Ca2+]i) via pertussis toxin-sensitive and -insensitive pathways. (uni-regensburg.de)
  • seroprevalence
  • In this study, our aim was to determine the seroprevalence of B. Pertussis among a group of adolescent first-year medical students in the province of Hamedan, Iran in order to provide local epidemiological data. (biomedcentral.com)
  • We compared the age-specific seroprevalence of various levels of IgG-PT in cross-sectional surveys using systematic collections of residual sera from Australian diagnostic laboratories in 1997/8, 2002 and 2007 with reference to both changes in the pertussis vaccine schedule and the epidemic cycle, as measured by disease notifications. (edu.au)
  • Population seroprevalence of high levels of IgG-PT is accepted as a reliable indicator of pertussis disease activity over time within and between countries with varying diagnostic practices, especially in unimmunised age groups. (edu.au)
  • treatment
  • There is evidence to suggest pertussis toxin can be useful therapeutic tool in the treatment of a wide variety of human ailments. (elisakits.co.uk)
  • The results suggest that in studies regarding the effect of pertussis toxin treatment on signal transduction, the toxin must be injected very close to the brain region of interest and, furthermore, that the rats should be killed 48-72 h after injection. (semanticscholar.org)
  • population
  • Based on this report, the incidence of pertussis in Iran has decreased from 40% in 100,000 population in 1978 to 0.5% in 100,000 population in 2007. (biomedcentral.com)
  • Campbell P, McIntyre P, Quinn H, Hueston L, Gilbert GL, McVernon J, Increased population prevalence of low pertussis toxin antibody levels preceding a record epidemic in Australia . (edu.au)
  • alpha
  • This is also demonstrated by comparison of the weak [32P]ATP-ribosylation of pertussis toxin substrates with the density of immunoreactive Gi alpha on Western blots in tissues such as lung. (biochemj.org)