• PAT1 is localized to the CV and the plasma membrane and is upregulated under conditions of Ca 2+ -stress [ 5 ]. (biomedcentral.com)
  • Dajumin-GFP allows to visualize the dynamics of the bladder by formation of irregular ventricles and ducts (A). The ER is prominently labeled in the perinuclear region and close to the plasma membrane (B). The cells were lysed by passage through nuclepore filters. (biomedcentral.com)
  • N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
  • Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. (exbio.cz)
  • van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. (exbio.cz)
  • Specific staining was localized to cytoplasm and plasma membrane. (rndsystems.com)
  • In the interfacial and headgroup regions of the membrane, the side chain free energy profiles show excellent agreement with profiles previously reported for conventional membranes with full tail lipids. (uiuc.edu)
  • There are at least six types of lipids including fatty acids, isoprenoids, sterols, phospholipids, glycosylphosphatidylinositol anchors, and lipid-derived electrophiles, which can be covalently attached to proteins ( Chen et al, 2018 ). (life-science-alliance.org)
  • We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
  • As a first step towards their molecular characterization, expression patterns by RT-PCR revealed thirteen genes that were detected exclusively and abundantly in chemosensory tissues. (unicamp.br)
  • Most genes were detected in chemosensory as well as non chemosensory tissues indicating that they might be encapsulins, but not necessarily olfactory proteins. (unicamp.br)
  • Known PPIs of the 62 MPM associated genes were derived from Biological General Repository for Interaction Datasets (BioGRID) and Human Protein Reference Database (HPRD). (cdc.gov)
  • The interactome is significantly enriched with genes differentially expressed in MPM tumors compared with normal pleura and with other thoracic tumors, genes whose high expression has been correlated with un-favorable prognosis in lung cancer, genes differentially expressed on crocidolite exposure, and ex-osomederived proteins identified from malignant mesothelioma cell lines. (cdc.gov)
  • Recent advances have been made in the of host factors, polymorphisms, and candidate genes associated characterization of the immune response to low-molecular- with occupational asthma may improve our understanding of weight agents. (cdc.gov)
  • He co-authored a paper that describes the NMR structure determination of an integral membrane protein enzyme, diacylglycerol kinase, a 100-kDa protein-detergent complex, which was published in Science . (vckd.org)
  • Western blots show that the K12 protein exists as a cluster of bands around 27 kDa, and extractions using nonionic detergents or high pH conditions demonstrate that it behaves as an integral membrane protein. (consensus.app)
  • Two sets of cDNAs were recovered, and evidence from three independent assays confirmed that both sets encoded articulins: (a) Anti-articulin antibodies recognized a high molecular weight beta-galactosidase (beta-gal) fusion protein expressed in bacteria infected with lambda gt11 cDNA clones. (rupress.org)
  • b) Antibodies generated against the bacterially expressed beta-gal fusion protein identified one or the other articulin in Western blots of Euglena proteins. (rupress.org)
  • These antibodies also localized to the membrane skeletal region in thin sections of Euglena. (rupress.org)
  • The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
  • Western blot and immunofluorescence assay (IFA) were used to examine the reactivity of rFg14-3-3e protein to anti- F. gigantica and anti-rFg14-3-3e antibodies, respectively. (biomedcentral.com)
  • Western blot analysis showed that rFg14-3-3e protein is recognized by sera from goats experimentally infected with F. gigantica and immunofluorescence staining using rat anti-rFg14-3-3e antibodies demonstrated the specific binding of rFg14-3-3e protein to the surface of goat PBMCs. (biomedcentral.com)
  • The polyclonal antibody E053 recognizes the cytoplasmic part of the epsilon subunits of the CD3 complex on thymocytes and on peripheral T lymphocytes and, in contrast to many monoclonal antibodies of the same specificity, is suitable for cryostat material and kerosene sections. (histoprime.com)
  • Purification of APP-AT protein complexes and identification of the constituents by mass spectrometry revealed several previously reported APP-interacting proteins as well as proteins involved in synaptic maintenance. (jneurosci.org)
  • Extensive characterization including infrared and X-ray spectroscopy, thermogravimetric analysis, size, charge, surface area, and density captured the alteration in physicochemical properties as the material went through sequential purification. (cdc.gov)
  • The extent of the elevator-like movement of the transport domain is so large (~20 Å in Glt Tk ) that HP2 acts as a gating element both on the extracellular and the intracellular side of the membrane. (nature.com)
  • For this purpose, WIN 55,212-2 was injected in pregnant wistar rats from gestation day 5 to 20 and a detailed analysis of the levels of the neurotrophin brain-derived neurotrophic factor (BDNF) as well as of the signaling molecules extracellular signal-regulated kinase (ERK)1/2 and alpha-calcium/calmodulin-dependent protein kinase II (alpha-CaMKII) was carried out in adult offspring. (researchgate.net)
  • The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. (exbio.cz)
  • Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis , traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. (springer.com)
  • The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. (biomedcentral.com)
  • Monné M, Chan KW, Slotboom DJ, Kunji ERS (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. (springer.com)
  • About 10% of eukaryotic proteins are supposed to be S-palmitoylated, identified by high-throughput screens (SwissPalm database) ( Blanc et al, 2015 ). (life-science-alliance.org)
  • Energetics of protein side chain partitioning between aqueous solution and cellular membranes is of fundamental importance for correctly capturing the membrane binding and specific protein lipid interactions of peripheral membrane proteins. (uiuc.edu)
  • We recently reported a highly mobile membrane mimetic (HMMM) model that accelerates lipid dynamics by modeling the membrane interior partially as a fluid organic solvent while retaining a literal description of the lipid head groups and the beginning of the tails. (uiuc.edu)
  • Protein lipidation is a unique co- or posttranslational modification in which lipid moieties are covalently attached to proteins. (life-science-alliance.org)
  • The transport domains are mobile and move through the lipid bilayer (alike an elevator) when translocating the amino acid substrate and co-transported ions across the membrane 13 . (nature.com)
  • Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). (tudelft.nl)
  • During the past decade our laboratory and others have shown that hereditary spherocytosis is caused by defects in the connections that attach the membrane skeleton to the overlying lipid bilayer. (dana-farber.org)
  • This indicates that AE1 and probably other integral membrane proteins have a 'lipid-anchoring' function. (dana-farber.org)
  • However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. (biomedcentral.com)
  • Our data suggest that rFg14-3-3e protein can influence various cellular and immunological functions of goat PBMCs in vitro and may be involved in mediating F. gigantica pathogenesis. (biomedcentral.com)
  • To overcome the complication that purified transporters may adopt right-side-out or inside-out membrane orientations upon reconstitution, thereby confounding the kinetic analysis, we employed a rapid method using synthetic nanobodies to inactivate one population. (nature.com)
  • Area A will delineate the diagnostic potential of TSPO imaging by means of positron emission tomography (PET) in brain tumors in vivo and will enable the complementary characterization of brain tissue in vitro to delineate the neurobiological characteristics underlying TSPO PET labeling in the human brain. (uni-regensburg.de)
  • To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
  • Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
  • Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)
  • A key approach is the application of confocal intravital microscopy that enables rigorous and direct means of investigating the interactions of leukocytes with different components of microvessel walls (endothelial cells, pericytes and the vascular basement membrane) in real-time in vivo. (qmul.ac.uk)
  • The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis . (springer.com)
  • Kunji ERS, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. (springer.com)
  • Identification, modeling, and characterization studies of Tetrahymena thermophila myosin FERM domains suggests a conserved core fold but functional differences.Cytoskeleton, 72: 585-596. (cuny.edu)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • Posttranslational protein S-palmitoylation regulates the localization and function of its target proteins involved in diverse cellular processes including meiosis. (life-science-alliance.org)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • We have previously reported the identification and characterization of an LKB1-specific adaptor protein, STRAD, which activates LKB1 and translocates it from nucleus to cytoplasm. (nih.gov)
  • In an attempt to understand the function of APP, in vitro studies have focused on the identification of interacting proteins. (jneurosci.org)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • An antibody prepared against a peptide sequence specific to the Aedes aegypti GPI-linked carbonic anhydrase labels plasma membranes of a number of cell types including neuronal cells and muscles. (silverchair.com)
  • Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
  • The long-term research goal of the lab is to apply computer modeling to gain insight into cellular signal transduction pathways, specifically to provide deeper insight into both the normal and aberrant subcellular targeting of domains contained in proteins which are part of macromolecular complexes and function in various biological processes. (cuny.edu)
  • LINC (linkers of nucleoskeleton and cytoskeleton) complexes might be involved in this process, due to their general function in bridging the NE and thereby physically connecting the nucleus to the peripheral cytoskeleton. (uni-wuerzburg.de)
  • LINC complexes consist of inner nuclear membrane integral SUN-domain proteins and outer nuclear membrane KASH-domain counterparts. (uni-wuerzburg.de)
  • To date, these protein complexes are known for their essential functions in nuclear migration, anchoring and positioning of the nucleus, and even for chromosome movements and the maintenance of cell polarity and nuclear shape. (uni-wuerzburg.de)
  • The alkaline gut is believed to disrupt and inhibit the formation of insoluble tannin-protein complexes. (silverchair.com)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • While the HMMM has been successfully applied to study spontaneous insertion of a number of peripheral proteins into membranes, a quantitative characterization of the energetics of membrane protein interactions in HMMM membranes has not been performed. (uiuc.edu)
  • In addition to the blood pressure criteria, proteinuria of greater than or equal to 0.3 grams in a 24-hour urine specimen, a protein (mg/dL)/creatinine (mg/dL) ratio of 0.3 or higher, or a urine dipstick protein of 1+ (if a quantitative measurement is unavailable) is required to diagnose preeclampsia. (medscape.com)
  • Fp-EVs upregulated the protein expression of zona occludens (ZO)-1 and Occludin and increased the ratio of Tregs in the colon tissue of colitis mice. (biomedcentral.com)
  • Tissue characterization shows a large area of subendocardial and mid-myocardial delayed enhancement that is most prominent in the LV apex, a laminated LV apical thrombus, and trivial circumferential pericardial effusion with evidence of diffuse pericardial inflammation. (medscape.com)
  • It is important to understand the functions and regulatory mechanisms of protein lipidation, which will advance our understanding of their pathological relevance, leading to strategies for targeting protein lipidation for therapeutic applications. (life-science-alliance.org)
  • TSPO is a ubiquitous mitochondrial protein and is involved in numerous biological functions. (uni-regensburg.de)
  • Our laboratory focuses on the organization and functions of the spectrin-based membrane skeleton. (dana-farber.org)
  • As an additional measure of the ability of the HMMM model to describe membrane protein interactions, the water to membrane interface transfer energies were analyzed and found to be in agreement with the previously reported experimental and computational hydrophobicity scales. (uiuc.edu)
  • In this study, we describe the isolation and characterization of nanobodies (Nb) recognizing multiple Campylobacter strains. (biomedcentral.com)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • Objective: the aim of this study was to describe a treatment for vestibuloplasty using a latex membrane. (bvsalud.org)
  • They form a multigenic family involved in the peripheral events in insect olfaction, specifically the transport of odorants to membrane-bound odorant receptors. (unicamp.br)
  • Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
  • CD3 is experimented by cortical thymocytes and by peripheral T lymphocytes. (histoprime.com)
  • We discuss strengths and weaknesses of HMMM in describing protein membrane interactions as well as further development of model membranes. (uiuc.edu)
  • Molecular modeling and computational analyses suggests that the Sinorhizobium meliloti periplasmic regulator protein ExoR adopts a superhelical fold and is controlled by a unique mechanism of proteolysis. (cuny.edu)
  • To understand eventually how these proteins assemble and function in maintaining cell form and membrane integrity we have undertaken a molecular characterization of articulins. (rupress.org)
  • Abstract The 90 kD heat shock proteins (Hsp90) are molecular chaperones that are responsible for the folding of select proteins, many of which are directly associated with cancer progression. (sbir.gov)
  • The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. (biomedcentral.com)
  • Fetal brain regions and peripheral tissues were examined for nAChR subtypes, other neurotransmitter receptors, and indices of cell signaling and cell damage. (nature.com)
  • GO enrichment analysis of the interacting proteins identified terms related to apoptosis, protein binding, locomotion, hippo signalling and leukocyte and lymphocyte differentiation, supporting the experimental findings. (biomedcentral.com)
  • Clark E A and Ledbetter J A. Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. (histoprime.com)
  • HuT 78, HT, CEMx174) and in primary human and nonhuman primate peripheral blood leukocyte cultures (13). (cdc.gov)
  • 60% of the peripheral membrane skeleton of Euglena gracilis consists of equimolar amounts of two proteins (articulins) with M(r)s in SDS gels of 80 and 86 kD. (rupress.org)
  • Ankyrins link integral membrane proteins to the spectrin-based membrane skeleton. (dana-farber.org)
  • Hereditary defects in membrane skeleton proteins. (dana-farber.org)
  • Although much experimental evidence indicates that AE1 is required for membrane skeleton assembly, AE1-/- red blood cells have a normal membrane skeleton. (dana-farber.org)
  • In addition, the CH domain occurs also in a number of proteins not known to bind actin, a notable example being the vav protooncogene. (embl.de)
  • Syntrophins are cytoplasmic peripheral membrane proteins that bind to components of mechanosenstive sodium channels and the extreme carboxy-terminal domain of dystrophin and dystrophin-related proteins. (antibodies-online.com)
  • Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
  • AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
  • Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
  • PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
  • Together, these results highlight the versatility of the cellular processes in which protein S-palmitoylation participates. (life-science-alliance.org)
  • Potential protein interactors of rFg14-3-3e were identified by querying the databases Intact, String, BioPlex and BioGrid. (biomedcentral.com)
  • 28 of the interactors of MPM proteins are targets of 147 U.S. Food and Drug Administration (FDA)approved drugs. (cdc.gov)
  • Arguelles J, Lee J, Cardenas LV, Govind S, Singh S. In Silico Analysis of a Drosophila Parasitoid Venom Peptide Reveals Prevalence of the Cation-Polar-Cation Clip Motif in Knottin Proteins. (cuny.edu)
  • c) Peptide maps of the beta-gal fusion protein were similar to peptide maps of Euglena articulins. (rupress.org)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • In mammalian cells, S-palmitoylate is added by a family of 23 transmembrane zinc finger DHHC (Asp-His-His-Cys)-containing protein acyl transferases and is removed by fatty acyl protein thioesterases ( Malgapo & Linder, 2021 ). (life-science-alliance.org)
  • From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)
  • PROJECT SUMMARY An estimated 30 million people in the United States suffer from some form of peripheral neuropathy, a condition that develops as a result of damage to the peripheral nervous system. (sbir.gov)
  • It belongs to a multimeric complex and is associated with other proteins.The pleiotropic actions of TSPO render this protein an interesting target in the nervous system. (uni-regensburg.de)
  • Area C will address the role of TSPO for neurodegeneration within the peripheral nervous system, the retina and the brain using newly established animal models and PET imaging. (uni-regensburg.de)
  • Halocins are particularly interesting because of the way the pore-forming bacteriocins have been used to probe cell membrane structure and the production and maintenance of energetic ion gradients across the membrane. (wikipedia.org)
  • The SUN-domain protein SUN4, which had received limited characterization prior to this work, was found to be exclusively expressed in haploid stages during germ cell development. (uni-wuerzburg.de)
  • The junctional proteins ZO-1 and p120 redistribute in a dotted circle peripheral to the brush border, in the absence of cell-cell contacts. (nih.gov)
  • Similarly, indices of cell loss (reduced DNA), cell size and neuritic outgrowth (protein/DNA and membrane/total protein ratios) were distinct for each region and did not necessarily follow the rank order of nAChR upregulation, suggesting the involvement of additional mechanisms such as oxidative stress. (nature.com)
  • This gene spans approximately 14 kb and encodes a 1.8-kb mRNA detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. (consensus.app)
  • His research focuses on red blood cell membrane disorders and anemias. (dana-farber.org)
  • The CD3 complex is experimented on the cell membrane of T lymphocytes. (histoprime.com)
  • The complex consists of several protein subunits (including gamma, delta, epsilon) associated with the T cell receptor (TCR). (histoprime.com)
  • The T cell receptor/CD3 complex: a dynamic protein ensemble. (histoprime.com)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • Consequently, inhibition of the Hsp90 protein folding machinery results in a combinatorial attack on numerous oncogenic pathways. (sbir.gov)
  • Detection of CXCR7/RDC‑1 in Human peripheral blood Monocytes by Flow Cytometry. (rndsystems.com)
  • The study provides support for continuing efforts towards early detection and characterization of haemoglobinopathies to control the affected births in Pakistan. (who.int)
  • Partitioning of amino acids into a model membrane: Capturing the interface. (uiuc.edu)
  • A number of actin-binding proteins, including spectrin, alpha-actinin and fimbrin, contain a 250 amino acid stretch called the actin binding domain (ABD). (embl.de)
  • Proteins containing only a single amino terminal CH domain. (embl.de)
  • The open reading frame predicts a 248-amino-acid protein with the hydropathic characteristics of a type 1a membrane protein. (consensus.app)
  • A role of Akr1 S-palmitoylating the nuclear fusion protein Tht1 to function in karyogamy is identified. (life-science-alliance.org)
  • Prokaryotic fusion protein corresponding to the human CD3 epsilon chain. (histoprime.com)
  • Immunohistochemical analysis of peripheral blood cells shows that K12 is found in leukocytes of the myeloid lineage, with the strongest staining observed in granulocytes and no detectable expression in lymphocytes. (consensus.app)
  • Immunohistochemical and immunochemical investigations confirmed these observations at the protein level. (researchgate.net)
  • 9. Insulin resistance and protein metabolism in chronic hemodialysis patients. (vckd.org)
  • To this end, the behavior and function of the testis-specific SUN4 protein was studied. (uni-wuerzburg.de)
  • E-cig users develop changes in lung function which manifest peripheral obstructive airway involvement (7). (researchsquare.com)
  • Posteroanterior and lateral views provide a gross view of atrial and ventricular size and shape and pulmonary vasculature, but additional tests are almost always required for precise characterization of cardiac structure and function. (msdmanuals.com)
  • rFg14-3-3e protein stimulated goat PBMCs to produce interleukin-10 (IL-10) and transforming growth factor beta (TGF-β), corresponding with low levels of IL-4 and interferon gamma (IFN-γ). (biomedcentral.com)
  • We have previously described the first cloning and partial characterization of carbonic anhydrase from larval Aedes aegypti mosquitoes. (silverchair.com)
  • We report here that the previously described carbonic anhydrase from Aedes aegypti is similar to mammalian CA IV in that it is a GPI-linked peripheral membrane protein. (silverchair.com)
  • Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). (embl.de)
  • Genome analysis and expression patterns of odorant-binding proteins from the Southern House mosquito Culex pipiens quinquefasciatus. (unicamp.br)
  • CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. (exbio.cz)
  • The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. (rndsystems.com)
  • Homology modelling revealed 65% identity between rFg14-3-3e and human 14-3-3 protein YWHAE. (biomedcentral.com)
  • Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
  • Flow cytometry analysis (surface staining) of CD36 in human peripheral blood with anti-CD36 (CB38) purified, GAM-APC. (exbio.cz)
  • Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). (exbio.cz)
  • Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227, filled histogram) or isotype control antibody (Catalog # MAB003 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). (rndsystems.com)
  • All the fluorinated compounds are unable to transfect RD-4 cells, if used alone, but they are all able to deliver a plasmid carrying an enhanced green fluorescent protein (EGFP) expression cassette, when co-formulated with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE) in a 1:2 ratio. (bvsalud.org)
  • We used dajumin-GFP expressing cells to obtain a fraction enriched in CV membranes and used this fraction to measure Ca 2+ -transport. (biomedcentral.com)
  • Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
  • In addition, an approximately 20-kDa soluble form of the K12 protein derived from the N-terminal domain is specifically secreted by cells into the culture medium. (consensus.app)
  • Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions. (biomedcentral.com)
  • No other peripheral cells experiment CD3 (except possibly Purkinje cells in the cerebellum). (histoprime.com)
  • For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)