Peripheral blood primitive hematopoietic stem cells. Medical search. Frequent questions

Detection of human hematopoietic cells in mouse bone marrow: Mice are euthanized under inhalation isoflurane anesthesia by cervical dislocation at or greater than 6 weeks post-transplantation. (andymatthewsphotography.com)
Smith and Broxmeyer, 1986), thereby making it a potential source of cells for transplantation (Bodger, 1987). (nationalacademies.org)
2003). After the early success of transplantation of cord blood from related donors, cord blood banks were established to provide rapidly accessible, human leukocyte antigen (HLA)-typed units predominantly for transplantation of HPCs from unrelated donors. (nationalacademies.org)
Blood and Marrow Transplant Research [CIBMTR] in 2004), the European Research Project on Cord Blood Transplantation (Eurocord) in 1993, and the Japanese Cord Blood Banking Network in 1996-expedited the clinical evaluation of the efficacy and safety of transplantation of cord blood from unrelated donors. (nationalacademies.org)
gene transfer, hematopoietic stem cells, lentiviral vectors, mesenchymal stem/progenitor cells, bone marrow transplantation, hematopoiesis Introduction HIV-based lentiviral vectors (LV) were proven to be capable of transducing a broad spectrum of nondividing cells in multiple mammalian species cIAP1 Ligand-Linker Conjugates 14 [1]. (eyesoftheelephants.com)
Many elderly patients cannot receive stem cell transplantation, so we hope that there can be a less toxic method to reduce disease recurrence, instead of just conducting medical observation and waiting for the disease to recur. (medicaltrend.org)
Hematopoietic stem cell transplantation (HSCT) is the only proven curative treatment for CML, but it is applicable in only a fraction of patients, Proglumide sodium salt mainly younger patients with a matched donor [7]. (rockstarsagainstliveearth.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
Together, both IST and stem cell transplantation are reasonable treatment options for patients with AA. (springermedizin.at)
European Group for Blood and Marrow Transplantation Severe Aplastic Anaemia Working Party. (springermedizin.at)
These are sometimes added when patients require additional therapy, such as stem cell transplantation, or when a temporary access situation develops (as when an indwelling central line is removed because of infection). (medscape.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Allogeneic blood cell transplantation following reduced-intensity conditioning is effective therapy for older patients with myelofibrosis with myeloid metaplasia. (uchicago.edu)
Long-term follow-up of nonmyeloablative allogeneic stem cell transplantation for renal cell carcinoma: The University of Chicago Experience. (uchicago.edu)
Impact of disease burden at time of allogeneic stem cell transplantation in adults with acute myeloid leukemia and myelodysplastic syndromes. (uchicago.edu)
Outcomes of patients with AML and MDS who relapse or progress after reduced intensity allogeneic hematopoietic cell transplantation. (uchicago.edu)
Allogeneic stem cell transplantation with alemtuzumab-based conditioning for patients with advanced chronic myelogenous leukemia. (uchicago.edu)
Performance status and comorbidity predict transplant-related mortality after allogeneic hematopoietic cell transplantation. (uchicago.edu)
Identification by random forest method of HLA class I amino acid substitutions associated with lower survival at day 100 in unrelated donor hematopoietic cell transplantation. (uchicago.edu)
Phase I-II study of clofarabine-melphalan-alemtuzumab conditioning for allogeneic hematopoietic cell transplantation. (uchicago.edu)
Pretreatment C-reactive protein is a predictor for outcomes after reduced-intensity allogeneic hematopoietic cell transplantation. (uchicago.edu)
Second autologous stem cell transplantation for relapsed lymphoma after a prior autologous transplant. (uchicago.edu)
Identifying familial myelodysplastic/acute leukemia predisposition syndromes through hematopoietic stem cell transplantation donors with thrombocytopenia. (uchicago.edu)
National Cancer Institute's First International Workshop on the Biology, Prevention, and Treatment of Relapse after Allogeneic Hematopoietic Stem Cell Transplantation: summary and recommendations from the organizing committee. (uchicago.edu)
Cord blood (CB) transplants offer several advantages namely the reduced need for HLA matching [thereby extending transplantation availability to nearly all patients (2)] and the decreased risk of chronic graft-versus-host disease the most important determinant of long-term quality of life in transplant patients. (gasyblog.com)
Because there is no effective chemotherapeutic regimen for intrathoracic malignant neurogenic tumor, all three patients received high-dose chemotherapy followed by hematopoietic stem cell transplantation. (springeropen.com)

Previous studies already showed that cell-intrinsic loss of WASp causes B cell tolerance and WASp deficiency in T helper (TH) cells is linked to negative effects on cytokine gene transcription necessary for TH1 differentiation. (bvsalud.org)
In the current study, we investigated the molecular mechanisms involved in WASp-mediated epigenetic regulation of B cell differentiation during NSCLC. (bvsalud.org)
Hodgkin's disease, ALCL and dendritic cell lines Commonly the assignment of any given hematopoietic cell line to a cell lineage and stage of arrested differentiation, based on its immunological and other phenotypes, does not present any problems. (andymatthewsphotography.com)
B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
Using aggregates of differentiating mouse embryonic stem cells called embryoid bodies, the authors plated cells in the differentiation timeline just prior to the arise of hematopoietic cells. (wikipedia.org)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
a) The cells should exhibits plastic adherence (b) The cell should possess specific set of cell surface markers, i.e. cluster of differentiation (CD) 73, D90, CD105 and lack expression of CD14, CD34, CD45 and human leucocyte antigen-DR (HLA-DR). (c) The cells should have the ability to differentiate in vitro into adipocyte, chondrocyte and osteoblast. (stemcellsgroup.com)
Leukemia occurs when a genetic mutation arises in a single cell that interferes with the normal maturation and differentiation of developing leukocytes. (pocketdrugguide.com)
in other words, the leukemic mutations may affect any stage of hematopoietic differentiation, and the type of leukemia can be characterized by the type of affected cell. (pocketdrugguide.com)
In chronic myelogenous leukemia, maturation arrest in myeloid differentiation is caused by a genetic mutation that results in increasing numbers of circulating myeloid cells: neutrophils, basophils, and eosinophils, collectively known as granulocytes (which are themselves a type of leukocyte). (pocketdrugguide.com)
One was a solid or isolated growth of oval primitive cells with Schwannian stroma, representative of a neuroblastoma (Figure 1 C). The second was a diffuse growth of large polygonal cells with ganglion cell differentiation and prominent Schwannian stroma, which was regarded as a ganglioneuroma. (springeropen.com)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
EPO supports the proliferation and differentiation of erythroid progenitor cells and is critical for their survival [ 1 ]. (biomedcentral.com)

Hemogenic endothelium Angioblast Endothelial progenitor cell Vasculogenesis Hemangioblastoma List of human cell types derived from the germ layers Basak GW, Yasukawa S, Alfaro A, et al. (wikipedia.org)
Figure 1 VCAM-1 + macrophages guide hematopoietic stem/progenitor cell to sites of definitive hematopoiesis. (amegroups.org)
There is growing evidence that resident progenitor cell populations exist in murine lung tissues and differentiate into a mesenchymal cell lineage [1, 2]. (keio.ac.jp)
No obvious correlations between clinical parameters, including age, karyotype, existence of paroxysmal nocturnal hemoglobinuria clones, pretreatment blood counts, progenitor cell counts, and the response to immunosuppressive therapy (IST), were found. (springermedizin.at)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
However CB transplants suffer from limited progenitor cell dose leading to delayed neutrophil engraftment and increased mortality (3 4 Recent studies in immunodeficient mice have confirmed the presence of human CB-derived long-term-repopulating hematopoietic stem cells (LT-HSCs) capable of regenerating the lifelong production of all mature blood cells (5). (gasyblog.com)
Unfortunately most growth systems available to date achieve progenitor cell growth at the expense of the LT-HSC AZD6244 (Selumetinib) loss (6) increasing the chance lately graft failure. (gasyblog.com)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
Progenitor cell (stem cell) lines in the bone marrow produce new blood cells and stromal cells. (medscape.com)

Hematopoiesis in the embryo As the embryo continues to develop, the hematopoiesis process moves to the liver, the spleen, and bone marrow, and begins producing other types of blood cells. (andymatthewsphotography.com)
In adults, hematopoiesis of red blood cells and platelets occurs primarily in the bone marrow. (andymatthewsphotography.com)
In the mouse embryo, the emergence of blood islands in the yolk sac at embryonic day 7 marks the onset of hematopoiesis. (wikipedia.org)
The first wave, defined as primitive hematopoiesis, takes place in the extraembryonic yolk sac and generates transitory hematopoietic cell populations consisting of primitive erythrocytes. (amegroups.org)
The second wave, referred to as definitive hematopoiesis, temporarily occurs in the fetal liver and transitions to the bone marrow and thymus, where multipotent hematopoietic stem cells (HSCs) contribute to the generation of all blood lineages ( 1 - 3 ). (amegroups.org)
In zebrafish, primitive hematopoiesis occurs at early somite stages in the intermediate cell mass (ICM) and the rostral blood island (RBI). (amegroups.org)
Despite these apparent differences in definitive hematopoiesis between fish and mammals, the definitive HSC niche functions to actively recruit stem cells and maintain their plasticity throughout adulthood ( 2 , 4 , 5 ). (amegroups.org)
There is growing evidence that during primitive hematopoiesis in the developing embryo that macrophages generated in the yolk sac are involved in the regulation of primitive HSC trafficking-a mechanism suggested to involve in the secretion of inflammatory factors, extracellular matrix (ECM) components, and metalloproteinases ( 3 , 9 , 10 ). (amegroups.org)
Primitive hematopoiesis occurs at early somite stages in the ICM and the RBI. (amegroups.org)
Subset of macrophages expressing ICAM-1 migrate to the sites of definitive hematopoiesis in AGM and CHT to recruit hematopoietic stem cells and initiate neonatal hematopoiesis and guide them further to sites of adult and definitive hematopoiesis in kidneys and thymus. (amegroups.org)
To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)
The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)

These committed progenitor cells are difficult to discern from the original multipotent cells but can be cultured to form colonies of specific types of blood cells (Guyton and Hall, 2000). (nationalacademies.org)
Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)
FIGURE 2-1 Formation of the multiple peripheral blood cells from multipotent hematopoietic stem cells. (nationalacademies.org)
Hemangioblasts are the multipotent precursor cells that can differentiate into both hematopoietic and endothelial cells. (wikipedia.org)
CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
Mesenchymal stem cells ( MSCs) are known to be multipotent stromal cells that can differentiate into a variety of cell types which include osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (stemcellsgroup.com)
Arnold Caplan (1991), coined the term "mesenchymal stem cell and stated that the cells as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies. (stemcellsgroup.com)
Fig. 1 Identification of previously unknown compounds promoting human CD34+ cell growth AZD6244 (Selumetinib) Optimization of fed-batch culture period indicated that the highest growth of multipotent progenitors and long-term culture-initiating cells (LTC-ICs) was obtained on day 12 (fig. S3 C AZD6244 (Selumetinib) to E). Similarly the proportion of apoptotic cells was lower at that time when compared with day 16 (fig. S3F). (gasyblog.com)

HPCs are immature cells that develop from hematopoietic stem cells, cells that can both self-renew and differentiate into hematopoietic progenitor cells. (andymatthewsphotography.com)
Hematopoietic stem cells (HSCs) have the capacity to self-renew and the potential to differentiate into all of the mature blood cell types. (andymatthewsphotography.com)
In addition cIAP1 Ligand-Linker Conjugates 14 to HSC, bone marrow contains mesenchymal stem/progenitor cells (MSC), which can differentiate into mature cells of multiple mesenchymal tissues cIAP1 Ligand-Linker Conjugates 14 including fat, bone, and cartilage. (eyesoftheelephants.com)
Hemangioblasts have been first extracted from embryonic cultures and manipulated by cytokines to differentiate along either hematopoietic or endothelial route. (wikipedia.org)
In the presence of the proper cytokines, a subset of these cells was able to differentiate into hematopoietic lineages. (wikipedia.org)
and the RBI cells are derived from the lateral mesoderm of the zebrafish head and differentiate into macrophages ( 4 - 8 ). (amegroups.org)
In the former model, tumor cells are hierarchically organized: a portion of cells, called the "stem cells" retain their ability to proliferate, while their offspring "differentiate" into nonproliferating cells [ 3 ]. (por-journal.com)
Similarly, they are characterized by an extensive capacity for self-renewal, proliferation, potential to differentiate into multiple lineages and their immune-modulatory role on various cells. (stemcellsgroup.com)
The blood cells mature and differentiate through a sequence of steps involving a series of complex - and incompletely understood - interactions with growth factors, cytokines, and other cells in the bone marrow. (pocketdrugguide.com)
proposed the "mononuclear phagocyte system" theory, by which tissue-resident macrophages were assumed to derive from blood-circulating monocytes and to differentiate within the host tissue ( 1 ). (frontiersin.org)
As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)

Flow cytometry data analysis of CD34+/CD133+ stem cells in bone marrow and peripheral blood and T, B, and NK cells after hematopoietic grafting. (tci-thaijo.org)
As patients continue to suffer from lymphoproliferative and myeloproliferative diseases known as haematopoietic malignancies can affect the bone marrow, blood, lymph nodes, and lymphatic and non-lymphatic organs. (bvsalud.org)
How to detect human hematopoietic cells in mouse bone marrow? (andymatthewsphotography.com)
Single-cell bone marrow suspensions are prepared by flushing the femurs and tibiae with PBS containing 2% FBS using a 21-gauge needle. (andymatthewsphotography.com)
Primitive human hematopoietic cells are enriched in cord blood compared with adult bone marrow or mobilized peripheral blood as measured by the quantitative in vivo SCID-repopulating cell assay. (andymatthewsphotography.com)
Our data provide evidence that adult stem cells in bone marrow can be efficiently transduced vector administration without preconditioning. (eyesoftheelephants.com)
Following intravenous administration of a first-generation LV into adult mice, we found that bone marrow exhibited the highest levels of transgene among nine organs examined, with more than 10% green fluorescent protein-positive (GFP+) cells detected in peripheral blood leukocytes (PBL) in these mice [9]. (eyesoftheelephants.com)
Bone marrow stem cells from adults have been viewed as the ideal target for gene- and cell-based therapy of genetic diseases, selected malignant diseases, and AIDS. (eyesoftheelephants.com)
These cells are thought to express both CD34 and CD133 These cells are likely derived from the bone marrow, and may even be derived from hematopoietic stem cells. (wikipedia.org)
Investigation of bone marrow cells showed that these became apoptotic within seven days after poly injection. (biomedcentral.com)
Committed myeloid progenitor cells and cells with long term reconstituting potential were lost from bone marrow within four days after poly injection. (biomedcentral.com)
Furthermore, infusion of normal bone marrow cells rescued mice from death induced by loss of THOC5/Fms interacting protein. (biomedcentral.com)
It is mainly characterized by abnormal proliferation of primitive and immature myeloid cells in bone marrow and peripheral blood. (medicaltrend.org)
Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)
NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
The definitive HSCs in mammals first migrate to and repopulate in the fetal liver and then home to the bone marrow, while the definitive HSCs in zebrafish migrate first to the caudal hematopoietic tissue and then to their definitive anatomical sites in the kidney and thymus ( 5 - 8 ). (amegroups.org)
In adults, HSCs residing in the bone marrow may become activated and mobilize into peripheral blood in response to ischemic injury or infection. (amegroups.org)
Multicolor flow cytometric analysis of Ly-76 (TER-119) expression on viable Mouse bone marrow cells. (bdbiosciences.com)
BALB/c Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. (bdbiosciences.com)
The bivariate pseudocolor density plot showing the correlated expression of Ly-76 (TER-119) [or Ig Isotype control staining] versus CD45 was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) bone marrow cells. (bdbiosciences.com)
Stem cell antigen-1 (Sca-1) is a cell surface glycoprotein, initially found in murine bone marrow-derived stem cell subtypes, such as hematopoietic stem cells. (keio.ac.jp)
Castration of normal male mice induces expansion of the bone marrow B cell population, an effect that can be reversed by androgen replacement. (jci.org)
Immature B cells from normal mouse bone marrow were not responsive to the suppressive effect of androgens unless they were cocultured with marrow stromal cells or with supernatants from androgen-treated stromal cells, suggesting that the androgen effects are exerted through marrow stromal elements by production of a diffusible mediator. (jci.org)
Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)
Several lines of evidence support the hypothesis that gonadal steroids exert effects on the development of B cells in bone marrow. (jci.org)
Androgens also exert effects on B cell development in bone marrow. (jci.org)
Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)
Patients are simultaneously given supportive care until their bone marrow achieves hematologic remission and is again producing normal hematopoietic cells. (medscape.com)
Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. (lecturio.com)
Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. (lecturio.com)
Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells. (lecturio.com)
They are found primarily in the bone marrow and also in small numbers in the peripheral blood. (lecturio.com)
Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
The first source of Mesenchymal stem cells was in the bone marrow and considered to be the gold standard for clinical research, although various other sources have being discovered which include: Adipose tissue, Dental pulp, Mobilised Peripheral blood, Amniotic fluid, Joint synovium, synovial fluid, Endosteum, Periosteum, Menstrual blood and birth-derived tissues. (stemcellsgroup.com)
Mature blood cells (red cells, white cells, and platelets) are normally produced in the bone marrow from primitive hematopoietic stem cells. (pocketdrugguide.com)
Once mature, the blood cells leave the bone marrow and enter the general circulation, where they have a limited life span. (pocketdrugguide.com)
The white blood cells proliferate without limitation, eventually replacing normal bone marrow cells and entering the peripheral bloodstream. (pocketdrugguide.com)
In addition to increasing the number of circulating granulocytes, chronic myelogenous leukemia affects the maturation of bone marrow stem cells and causes an increase in blasts in the bloodstream and marrow. (pocketdrugguide.com)
Cell biology of phagocytosis has been mainly established on bone marrow-derived tissue macrophages. (frontiersin.org)
Particularly, yolk sac-derived macrophages including microglia may possibly have different tasks since they are confronted with different target structures, mainly apoptotic cells during developmental tissue remodeling, while bone marrow-derived macrophages have a higher chance to be confronted with pathogens, mainly during defense against invading microbes. (frontiersin.org)
Bone marrow consists of stem cells, which are large, "primitive," undifferentiated cells supported by fibrous tissue called stroma. (medscape.com)
There are 2 main types of stem cells and, therefore, the bone marrow consists of 2 types of cellular tissue. (medscape.com)
Bone marrow can be 1 of 2 types, red or yellow, depending on whether it consists of mainly hematopoietic (and therefore, red-colored) tissue or fatty (and therefore a yellow-colored) tissue. (medscape.com)
Both types of bone marrow are highly vascular, being enriched with numerous blood vessels and capillaries. (medscape.com)
Bone marrow supersedes the liver as the major hematopoietic organ at 32-36 weeks' gestation. (medscape.com)
However, the yellow marrow can revert to red if there is increased demand for red blood cells, such as in instances of blood loss. (medscape.com)
Normally, only mature cells are released from the marrow into the bloodstream. (medscape.com)
These stem cells divide to eventually give rise to red blood cells, platelets, and most white blood cells in the red marrow. (medscape.com)
Bone marrow thus contains blood cells at varying stages of development. (medscape.com)
Illustration of the pelvis to show the site of bone marrow and blood cells derived from bone marrow. (medscape.com)
When the oxygen content of body tissues is low, if there is loss of blood or anemia, or if the number of red blood cells decreases, the kidneys produce and release erythropoietin, a hormone that stimulates the bone marrow to produce more red blood cells. (medscape.com)
Similarly, the bone marrow produces and releases more white blood cells in response to infections, and it produces and releases more platelets in response to bleeding. (medscape.com)
If a person experiences serious blood loss, yellow bone marrow can be activated and transformed into red bone marrow. (medscape.com)
As age progresses, more of the red bone marrow turns into yellow bone marrow and the production of new blood cells becomes more difficult. (medscape.com)
The bone marrow stroma contains mesenchymal stem cells. (medscape.com)

Lineage Negative (Lin-) Markers Note A select list of Lin- markers are provided as examples of markers that have been used in the literature to exclude particular lineages of cells in order to help identify the cell type of interest. (andymatthewsphotography.com)
shows the different lineages of blood cells and the stages involved in their maturation. (pocketdrugguide.com)

The aim of this research was to study the effects of diagnostic X-rays on peripheral blood mononuclear cells (PBMCs) and CD34 + /CD133 + populations in healthy volunteers in vitro and determine the ratio of CD34 + /CD133 + in PBMCs. (tci-thaijo.org)
The overall results indicated that increasing the radiation dose had significant effects on PBMCs and the CD34 + /CD133 + population of cells. (tci-thaijo.org)
Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44. (tci-thaijo.org)
It has been shown that these pre-endothelial/pre-hematopoietic cells in the embryo arise out of a phenotype CD34 population. (wikipedia.org)
The hypothesis of a bipotential precursor was further supported by the fact that endothelial cells and hematopoietic cells share many of the same markers, including Flk1, Vegf, CD34, Scl, Gata2, Runx1, and Pecam-1. (wikipedia.org)
To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Control (dimethyl sulfoxide DMSO) fed-batch cultures contained mostly differentiated cells (Fig. 2A DMSO) and a reduced frequency of CD34+CD45RA? (gasyblog.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)

In summary, we identify myostatin propeptide as a novel positive regulator of primitive CML cells and corresponding normal hematopoietic cells. (haematologica.org)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Cell division tracking further showed that UM171 does AZD6244 (Selumetinib) not impact the division rate of phenotypically primitive populations (fig. S7). (gasyblog.com)
Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)

Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients. (celljournal.org)
Cancerous cells are embedded into diverse tissues consisting of cancer-associated fibroblasts, extracellular matrix, vascular and lymphatic networks, and immune cells, among others. (por-journal.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
Mesenchymal stem/stromal cells (MSCs) can be isolated from neonatal tissues, most of which are discarded after birth, including placental tissues, fetal membranes, umbilical cord, and amniotic fluid. (stemcellsgroup.com)
Thus, neonatal tissues are easily available and they have biological advantages in comparison to adult sources that make them a useful source for stem cells including MSCs. (stemcellsgroup.com)
Treating such diseases remains difficult and several strategies have been used to stimulate the growth of blood vessels and promote regeneration of ischaemic tissues, such as the use of recombinant proteins and gene therapy. (biomedcentral.com)
In vascular medicine, various stem cells and adult progenitors have been highlighted as having a vasoreparative role in ischaemic tissues. (biomedcentral.com)
Ischaemia is characterised by a reduction in oxygen supply to tissues and organs, usually as a result of blood vessel constriction or obstruction. (biomedcentral.com)
The evaluation of the most suitable timing of cell delivery as well as the number of cells needed to integrate into resident vasculature and promote revascularisation of specific tissues requires careful optimisation and evaluation. (biomedcentral.com)

The hematopoietic cascade: development of mature blood cells from pluripotent stem cells. (pocketdrugguide.com)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)

Low-dose radiation does not induce proliferation in tumor cells in vitro and in vivo. (tci-thaijo.org)
The translocation occurs in a primitive hematopoietic stem cell and the gene encodes a fusion protein (Bcr-Abl) that has deregulated tyrosine kinase (TK) activity and activates intracellular pathways that lead to increased cellular proliferation, resistance to apoptosis and genetic instability [1,3]. (rockstarsagainstliveearth.com)
Importantly, subsequent dose-response analyses revealed an age-associated reduction in the growth factor-stimulated proliferation of CD49f + cells mediated by reduced activation of AKT and altered cell cycle entry and progression. (ashpublications.org)
These findings identify a new intrinsic, pervasive, and progressive aging-related alteration in the biological and signaling mechanisms required to drive the proliferation of very primitive, normal human hematopoietic cells. (ashpublications.org)
The word leukemia derives from the Latin word for white blood and refers to the proliferation of white blood cells (leukocytes) in people with this disease. (pocketdrugguide.com)
We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)

Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)

By using limiting dilutions, the authors demonstrated that the resulting hematopoietic and endothelial cells were indeed of clonal origin, proving that they had successfully isolated the hemangioblast in the developing embryo. (wikipedia.org)
1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
There are two not mutually exclusive models explaining tumor heterogeneity: the cancer stem cell and the clonal evolution model. (por-journal.com)
Acute myeloid leukemia (AML) is a heterogeneous disease in terms of genetic basis, clinical, biological and prognostic, and is a malignant clonal disease of leukemia stem cells (LSCs). (biomedcentral.com)

Transplanted tissue may be cells (e.g., hematopoietic stem cells Hematopoietic stem cells Progenitor cells from which all blood cells derived. (lecturio.com)
Thus, these properties of MSCs make these cells potentially ideal candidates for tissue technology. (stemcellsgroup.com)
If the underlying ischaemia is not sufficiently resolved it can lead to tissue damage, with subsequent cell death. (biomedcentral.com)
This review will examine the clinical potential of several stem and progenitor cells that may be utilised to regenerate defunct or damaged vasculature and restore blood flow to the ischaemic tissue. (biomedcentral.com)
This leads to hypoxia and tissue damage as a consequence of the build up of waste metabolites and may result in cell death [ 1 ]. (biomedcentral.com)
Cells for vascular therapy must be able to home to ischaemic or damaged tissue and engage in vessel formation alone or in unison with resident vasculature to achieve a controlled and functional reperfusion event, without causing pathological angiogenesis (for example, proliferative retinopathy in the vitreous of the eye). (biomedcentral.com)
A cell therapy approach should be aimed at promoting revascularisation of ischaemic tissue. (biomedcentral.com)
There is a therapeutic window in which to deliver the cells, to avoid extensive tissue damage, fibrosis and necrosis. (biomedcentral.com)
They are often studied independently from other tissue-resident macrophages, probably because they are unique in some aspects, most notably in their isolation from the rest of the body through the blood brain barrier (BBB). (frontiersin.org)
The tissue protective functions of the hematopoietic growth factor erythropoietin (EPO) are independent of its action on erythropoiesis. (biomedcentral.com)

Non-Vascularised Ipsilateral Fibular strut - A Modality to Treat Giant cell tumor of lower end radius using Anterior Approach. (jbstjournal.com)
Giant cell tumor is a benign bone tumor, locally aggressive with low malignant potential. (jbstjournal.com)
Juxta-articular giant cell tumors of the lower end radius are common and present a special problem of reconstruction after tumor excision. (jbstjournal.com)
Giant cell tumor (GCT) of bone is a benign but locally aggressive tumor with tendency for local recurrence [2]. (jbstjournal.com)
We studied 5 consecutive cases of GCT involving the distal radius operated by en-bloc resection of tumor followed by reconstruction with ipsilateral non-vascularized fibular graft with a minimum 1 year follow-up. (jbstjournal.com)
Zhang Y, Zhang Y, Chen J, Liu Y, Luo W. Dendritic-tumor fusion cells derived heat shock protein70-peptide complex has enhanced immunogenicity. (celljournal.org)
In recent years, the evolution of the molecular biological technical background led to the widespread application of single-cell sequencing, a versatile tool particularly useful in the investigation of tumor heterogeneity. (por-journal.com)
Nowadays, with single-cell approaches, tumor heterogeneity, the tumor microenvironment, and the interplay between tumor cells and their surroundings can be described in unprecedented detail. (por-journal.com)
In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)
Tumor heterogeneity means that neoplastic cells from the same tumor can genotypically, phenotypically, morphologically, or metabolically differ from each other. (por-journal.com)
Some of these cells have antitumoral behavior (NK cells, CD8 + T cells, CD4 + Th1 cells, and APCs), while others can promote tumor progression (CD4 + Th2 cells, regulatory T cells, and tumor-associated macrophages) [ 13 ]. (por-journal.com)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
The tumor nest was composed of primitive cells with round or oval hyperchromatic nuclei and scant cytoplasm (high-power view). (springeropen.com)

Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
Macroglia - astrocytes and the oligodendrocyte lineage - arise from neuroepithelial progenitor cells (NPCs) in the embryonic neural tube and forebrain ( Rowitch and Kriegstein, 2010 ). (biologists.com)
At around embryonic day (E) 9 in mice, NPCs transform into radial glia, which are the primary progenitor cells for both neurons and macroglia during embryogenesis. (biologists.com)

Low dose radiation induced senescence of human mesenchymal stromal cells and impaired the autophagy process. (tci-thaijo.org)
Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)
Androgens only affected B cell development in chimeric mice with androgen-sensitive stromal cells. (jci.org)
One type of stem cell is involved in producing blood cells and the other is involved in producing stromal cells, which are responsible for the supporting stroma. (medscape.com)

Placenta is an ideal starting material for the large-scale manufacture of multiple cell doses of allogeneic MSC. (stemcellsgroup.com)
Phase I study of dose-escalated busulfan with fludarabine and alemtuzumab as conditioning for allogeneic hematopoietic stem cell transplant: reduced clearance at high doses and occurrence of late sinusoidal obstruction syndrome/veno-occlusive disease. (uchicago.edu)
BK virus infection is associated with hematuria and renal impairment in recipients of allogeneic hematopoetic stem cell transplants. (uchicago.edu)
Allogeneic stem cell transplant in renal failure: engraftment and prolonged survival, but high incidence of neurologic toxicity. (uchicago.edu)
T-cell-depleted allogeneic transplant without donor leukocyte infusions results in excellent long-term survival in patients with multiply relapsed Lymphoma. (uchicago.edu)

Human hematopoietic stem cells (HSCs), like their counterparts in mice, comprise a functionally and molecularly heterogeneous population of cells throughout life that collectively maintain required outputs of mature blood cells under homeostatic conditions. (ashpublications.org)
HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
These HSCs are primarily derived from endothelial cells within the ventral wall of the dorsal aorta. (amegroups.org)
SAGA complex member expression is significantly reduced in aged HSCs and upregulated with heterochronic parabiosis, suggesting a novel mechanism of age-associated hematopoietic decline and rejuvenation. (biorxiv.org)
These LT-HSCs show a delayed engraftment pattern in opposition to short-term HSCs (ST-HSCs) that produce short-lived progenitors responsible for the production of mature blood cells and prompt neutrophil recovery (3 5 Hence there is great interest in the development of conditions for robustly expanding these progenitor cells while maintaining or expanding LT-HSCs. (gasyblog.com)
mobilized peripheral bloodstream (mPB) cells that are enriched in LT-HSCs (10) (fig. S1 B) and A. Seven hits had been determined after excluding the autofluorescent substances (Fig. 1A and fig. S1C) five which had been known [four (11 12 or previously unidentified (one UM125454 fig. S2) suppressors from the AhR pathway (Fig. 1B). (gasyblog.com)
cells (10) (Fig. 1D and fig. S3 B) and A. UM729 didn't broaden mouse HSCs (fig. S4). (gasyblog.com)

There is now emerging evidence of hemangioblasts that continue to exist in the adult as circulating stem cells in the peripheral blood that can give rise to both endothelial cells and hematopoietic cells. (wikipedia.org)
Furthermore, it was shown that depletion of Flk1 in the developing embryo results in disappearance of both hematopoietic cells and endothelial cells. (wikipedia.org)
In addition, these same cells can also be differentiated into endothelial cells, as shown by Choi of the Keller Lab. (wikipedia.org)
In particular, we focus on the therapeutic potential of endothelial progenitor cells as an exciting new option for the treatment of ischaemic diseases. (biomedcentral.com)
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
Peripherally administered EPO crosses the blood-brain barrier and activates in the brain anti-apoptotic, anti-oxidant and anti-inflammatory signaling in neurons, glial and cerebrovascular endothelial cells and stimulates angiogenesis and neurogenesis. (biomedcentral.com)

VCAM-1, vascular cell adhesion molecule-1. (amegroups.org)
Some studies showed Sca-1 expression in the pulmonary vascular endothelium of adult murine lungs [3], while a subset of Sca-1-expressing cells formed vascular-like structures under specific conditions [1]. (keio.ac.jp)
The use of cell therapy for vascular regeneration offers an exciting new prospect in regenerative medicine. (biomedcentral.com)
However, the delivery of the correct cell type to the precise area of injury or vascular insufficiency is difficult and many factors need to be considered. (biomedcentral.com)
These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)

During this first wave, small number of myeloid cells (e.g., primitive monocytes/macrophages and megakaryocytes) are also generated. (amegroups.org)
Although CD4 T cells are the main targets of HIV infection, macrophages also become infected and resist the cytopathic effects of infection, contributing potentially to HIV reservoir persistence. (nih.gov)
In multicellular organisms, and more particularly in animals with a well-developed immune system, phagocytosis is mostly performed by specialized, professional phagocytes: macrophages, dendritic cells (DCs), and neutrophils. (frontiersin.org)

Hemangioblasts are the progenitors that form the blood islands. (wikipedia.org)

Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)

Acute myeloid leukemia (AML) is a malignant disease of myeloid hematopoietic stem/progenitor cells. (medicaltrend.org)
Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. (celljournal.org)
Treatment for patients with acute myeloid leukemia involves intensive chemotherapy to destroy the leukemic cell population as rapidly as possible and to prevent the emergence of a resistant clone. (medscape.com)
The concept of heterogeneity has been around for several decades and gained attention in the 1990s when cancer stem cells were identified in acute myeloid leukemia [ 2 ]. (por-journal.com)

Ex vivo Hsp70-activated nk cells in combination with pd-1 inhibition significantly increase overall survival in preclinical models of glioblastoma and lung cancer. (celljournal.org)

In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
With more than 6,000 transplants of cord blood from related and unrelated donors performed thus far, cord blood has emerged as an acceptable, alternative source of HPCs that has some advantages over adult sources of HPCs and the availability of which represents an important development in the field. (nationalacademies.org)
Microglia, the resident immune cells in the CNS, have been studied extensively with regard to their roles in inflammation and disease, but less is known about their functions in the developing and adult brain ( Casano and Peri, 2015 ). (biologists.com)
Recent data, however, has shown that unchallenged microglia phagocytose apoptotic cells during development and in adult neurogenic niches, suggesting an overlooked role in brain remodeling throughout the normal lifespan. (frontiersin.org)
However, they have a different origin and are a unique macrophage cell type in the adult organism. (frontiersin.org)
EPO and its receptors (EPOR) are expressed in multiple brain cells during brain development and upregulated in the adult brain after injury. (biomedcentral.com)

Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
White blood cells last anywhere from a few hours to a few days, platelets for about 10 days, and red blood cells for about 120 days. (medscape.com)

Are primitive human hematopoietic cells enriched in cord blood? (andymatthewsphotography.com)
In the last decade, the number of transplantations of HPCs derived from cord blood has increased, particularly for children. (nationalacademies.org)
Since then cord blood banking programs throughout the world have expanded rapidly (Broxmeyer, 1998), with the estimated number of units stored to date exceeding 155,000 (BMDW, 2004). (nationalacademies.org)
The small amount of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. (gasyblog.com)

Latest studies demonstrated that aryl hydrocarbon receptor (AhR) antagonists and a notch ligand agonist promote the in vitro enlargement of individual CB cells with repopulating activity long lasting up to 16 weeks in immunodeficient mice (7 8 We created an computerized and continuous moderate delivery program that creates an equivalent enlargement of CB cells with equivalent repopulation properties (9). (gasyblog.com)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)

PEGylated G-CSF (BBT-015), GM-CSF (BBT-007), and IL-11 (BBT-059) analogs enhance survival and hematopoietic cell recovery in a mouse model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)
They can also be used for increasing white blood cell survival and for chemotherapy. (justia.com)

Most patients (90%) are diagnosed in chronic phase (CP), characterized by an increase in white blood cell counts with immature granulocytes in the peripheral blood, and up to 40% are asymptomatic at time of diagnosis. (rockstarsagainstliveearth.com)

Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)
There are many benefits for patients from medical imaging but X-rays can damage the cells in human body. (tci-thaijo.org)
Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells. (tci-thaijo.org)
Characteristics of human CD34+ cells exposed to ionizing radiation under cytokine-free conditions. (tci-thaijo.org)
Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
The development of the human blood-CSF-brain barrier. (cdc.gov)

Here, we report the clinical case of an 18-month-old boy diagnosed with Wiskott-Aldrich syndrome, who did not have an HLA-matched related or unrelated donor and was treated successfully with a hematopoietic stem cell transplant (HSCT) from a haploidentical family donor. (bvsalud.org)
In this review, we described the structure and function of WASp and its family mechanism, analysing major regulatory effectors and summarising the clinical relevance and drugs that specifically target WASp in disease treatment in various hematopoietic malignancies by different approaches. (bvsalud.org)
This global clinical trial was led by Professor Andrew Wei of the Australian Centre for Blood Diseases of Monash University and a hematologist at Alfred Health Center, and the research team published a report entitled "Oral Azacitidine Maintenance Therapy" in the New England Journal of Medicine. (medicaltrend.org)
Giant cell tumors (GCT) of the bone are aggressive and are recognised for variable clinical behaviour, which is not always related to radiographic or histological appearance [1]. (jbstjournal.com)
In the chronic phase of the disease, these cells are functionally mature and the initial clinical features of the disease are a result of high levels of these granulocytes. (pocketdrugguide.com)

Phase II study of immunomodulation with granulocyte-macrophage colony-stimulating factor, interleukin-2, and rituximab following autologous stem cell transplant in patients with relapsed or refractory lymphomas. (uchicago.edu)
Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)
Because it is such a diverse population of cells, a fundamental question is whether they have a common precursor or whether each macrophage population develops from a different precursor. (frontiersin.org)

Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
After radial glial cells generate neurons, a 'gliogenic switch' occurs and they begin differentiating into astrocytes or oligodendrocyte precursor cells (OPCs, also called NG2 glia). (biologists.com)
an increase in the number of myeloid white blood cells occurs, particularly early myeloid forms. (pocketdrugguide.com)

Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
Leukemias are cancers of the hematopoietic (blood-producing) system. (pocketdrugguide.com)

From these blood islands, the hematopoietic cells and vasculature are formed shortly after. (wikipedia.org)

Since then, with the evolution of the equipment and toolbox of molecular biology with methods such as single-cell next generation sequencing (NGS), the need for an even more detailed investigation of organisms at a single-cell level has emerged. (por-journal.com)
CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)

Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)
A pro-inflammatory environment is thought to provide a selective advantage for the leukemic stem cells (LSC). (haematologica.org)

however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
Initial experiments revealed that advancing donor age was accompanied by a significant and progressively delayed proliferative response but not the altered mature cell outputs seen in normal older individuals. (ashpublications.org)
When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)

Establishing a murine model of the hematopoietic syndrome of the acute radiation syndrome. (iu.edu)

The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)

There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)

This is defined by their ability to repopulate an individual whose hematopoietic system has been destroyed by radiation or chemotherapy. (justia.com)

First, maintaining stem cell properties including repopulation potential during culture is usually a prerequisite of any successful gene transfer approach. (eyesoftheelephants.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
The expression level of NCALD gene was associated with the prognosis of ovarian cancer and non-small cell lung cancer (NSCLC). (biomedcentral.com)

In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)

Effects of oxygen metabolites/chemokines on hematopoietic stem cell expansion and preservation. (rochester.edu)

Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)
The common stem cell produces 2 other stem cells, the myeloid stem cell and the lymphoid stem cell. (medscape.com)

The hematopoietic stem compartment consists of a limited number of discrete stem cell subsets. (andymatthewsphotography.com)
Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)

Anatomy57

Organisms14

Diseases27

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Psychiatry and Psychology1

Phenomena and Processes47

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups3

Health Care5

Transplantation30

Differentiation17

Progenitor cell10

Hematopoiesis12

Multipotent8

Differentiate12

Marrow64

Lineages2

CD3412

Normal hematopoietic cells1

Populations3

Tissues9

Pluripotent stem2

Proliferation6

Long-term hematopoietic1

Clonal4

Tissue10

Tumor13

Embryonic3

Stromal cells4

Allogeneic5

HSCs7

Endothelial6

Vascular5

Macrophages3

Progenitors1

Chronic myeloid1

Acute myeloid4

Vivo1

Adult6

Platelets2

Cord4

Vitro2

Survival2

Immature1

Human11

Clinical5

Macrophage3

Occurs3

Cancers2

Vasculature1

Biology2

Leukemic2

Proliferative5

Murine1

Malignant1

Maturation2

Chemotherapy1

Gene3

Successfully1

Chemokines1

Lymphoid2

Subsets2