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  • mononuclear cells
  • the CFU-Hill population is an early outgrowth, formed by plating peripheral blood mononuclear cells on fibronectin-coated dishes, allowing adhesion and depleting non-adherent cells, and isolating discrete colonies. (wikipedia.org)
  • However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
  • adipose tissue
  • The similarities between stem cells extracted from the bone marrow and the adipose tissue suggest the potential for the adipose tissue to act as an alternative, and perhaps preferable, cell source for repairing damaged tissues, such as the ischemic or infarcted heart. (ahajournals.org)
  • acute
  • PLX-R18 were initially developed with Prof Raphael Gorodetsky at Hadassah Hospital for the treatment of acute radiation syndrome and enhancement of bone marrow regeneration. (wikipedia.org)
  • Some experimental treatments, including systemic hypothermia, have been performed in isolated cases in order to draw attention to the need for further preclinical and clinical studies to help clarify the role of hypothermia in acute spinal cord injury. (wikipedia.org)
  • differentiate
  • citation needed] Possible mechanisms of recovery include: Generation of heart muscle cells Stimulation of growth of new blood vessels to repopulate damaged heart tissue Secretion of growth factors Assistance via some other mechanism It may be possible to have adult bone-marrow cells differentiate into heart muscle cells. (wikipedia.org)
  • Hematopoietic cells are a type of adult (i.e., non-embryonic) stem cell that can multiply and differentiate into the three types of blood cells: red blood cells, white blood cells, and platelets. (wikipedia.org)
  • Stem cells from the bone marrow, which is derived from mesoderm, can differentiate into liver, lung, GI tract and skin, which are derived from endoderm and mesoderm. (wikipedia.org)
  • While the terms mesenchymal stem cell and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
  • stromal cells
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • Culturing marrow stromal cells in the presence of osteogenic stimuli such as ascorbic acid, inorganic phosphate and dexamethasone could promote their differentiation into osteoblasts. (wikipedia.org)
  • radiation
  • Investigators are investigating whether a new conditioning regimen that uses less intensive drugs (treosulfan and fludarabine phosphate) with or without low dose radiation results in new blood-forming cells (engraftment) of the new donor cells without increased toxicities in patients with nonmalignant (noncancerous) diseases. (clinicaltrials.gov)
  • clinical
  • As one of Hadassah University's senior scientists, Prof. Or continues to conduct research on various clinical topics, including the prevention of liver complications following bone marrow transplantation and immunotherapy using donor derived lymphocytes (immune cells) for the eradication of malignant diseases that would otherwise prove fatal. (isracast.com)
  • However, the relatively low abundance, small tissue volume, difficult accessibility, and disease-related malfunction of bone marrow-derived stem cells hamper their clinical usefulness. (ahajournals.org)
  • For instance, bone marrow aspiration may yield low numbers of stem cells on processing, which necessitates an ex vivo expansion step to obtain sufficient cell numbers for clinical application. (ahajournals.org)
  • High-dose immunosup-pression and hematopoietic stem cell transplantation in autoimmune disease: clinical review. (springer.com)
  • Clinical and animal studies have been conducted into the use of stem cells in cases of spinal cord injury. (wikipedia.org)
  • However, aside from methylprednisolone, none of these developments have reached even limited use in the clinical care of human spinal cord injury in the US. (wikipedia.org)
  • Persons with mucolipidosis may share some of the clinical features associated with the mucopolysaccharidoses (certain facial features, bony structure abnormalities, and damage to the brain), and increased amounts of the enzymes needed to break down the lipids are found in the blood. (wikipedia.org)
  • cells
  • The laboratory's team, headed by Prof. Reuven Or, includes a number of highly respected and accomplished scientists who conduct advanced research with a focus on the immuno-biological mechanism of stem cells derived from cord blood and bone marrow. (isracast.com)
  • This process replaces unhealthy bone marrow/stem cells with healthy cells. (spectrumhealth.org)
  • A blood transfusion may be needed for children with serious or chronic forms of anemia, to increase their red blood cells and introduce healthy red blood cells to their bodies. (spectrumhealth.org)
  • The body's normal cells that are most sensitive to destruction by high-dose therapy are the blood-producing stem cells in the bone marrow. (louisianacancer.com)
  • When high-dose therapy is used to treat cancer, one of the major side effects is destruction of the stem cells living in the bone marrow. (louisianacancer.com)
  • Stem cells may be collected from the bone marrow, peripheral blood or umbilical cord. (louisianacancer.com)
  • Collection of blood stem cells. (assuta.clinic)
  • Infusion, transplantation of stem cells. (assuta.clinic)
  • The patient's own stored stem cells are then transfused into his/her bloodstream, where they replace destroyed tissue and resume the patient's normal blood cell production. (wikipedia.org)
  • The purpose of the conditioning therapy is to destroy the patient's abnormal bone marrow which doesn't work properly in order to make way for the new healthy donor cells which functions normally. (clinicaltrials.gov)
  • Peripheral blood stem cells were obtained by leukapheresis after mobilization with granulocyte colony-stimulating factor. (springer.com)
  • Hematopoietic stem cells give rise to blood cells. (wikipedia.org)
  • The hematopoietic cells are able to go to the bone marrow through tracking mechanisms. (wikipedia.org)
  • GM-CSF stimulates production of white blood cells. (wikipedia.org)
  • These agents, however, cannot discriminate between the leukaemia or neoplastic cells, and the hematopoietic stem cells within the bone marrow. (wikipedia.org)
  • It is an allogenic stem therapy based on mesenchymal stem cells (MSCs) derived from the bone marrow of adult donors. (wikipedia.org)
  • Although such attempts produced no positive benefit, further research found in the mid twentieth century that human cells could be used to help prevent the human body rejecting transplanted organs, leading in time to successful bone marrow transplantation. (wikipedia.org)
  • Hematopoiesis is the process by which all mature blood cells are produced. (wikipedia.org)
  • It must balance enormous production needs (more than 500 billion blood cells are produced every day) with the need to precisely regulate the number of each blood cell type in the circulation. (wikipedia.org)
  • The cells can be removed as liquid (to perform a smear to look at the cell morphology) or they can be removed via a core biopsy (to maintain the architecture or relationship of the cells to each other and to the bone). (wikipedia.org)
  • Using the ratio ρ = L / M {\displaystyle \rho =L/M} of lymphoid (L) to myeloid (M) cells in blood as a quantitative marker, the stem cell compartment can be split into three categories of HSC. (wikipedia.org)
  • In May 2012, Pluristem reported that its experimental PLacental eXpanded cells were injected into the muscles of a 7-year-old Romanian girl undergoing treatment for bone marrow aplasia disease at the Hadassah Medical Center in Jerusalem. (wikipedia.org)
  • Two months thereafter, with the patient's condition deteriorating rapidly, the Director of Bone Marrow Transplantation, Cell Therapy and Transplantation Research Center at Hadassah felt that all available options had been exhausted and turned to Pluristem's PLX cells. (wikipedia.org)
  • According to Pluristem CEO Zami Aberman, it was the first time ever that stem cells were injected into the muscle rather than into the body's blood system. (wikipedia.org)
  • Although bone marrow-derived cells do appear to localize to injured vessels and promote an angiogenic switch, other studies have suggested these cells do not contribute directly to the functional endothelium, instead acting via paracrine methods to provide support for the resident endothelial cells. (wikipedia.org)
  • A similar method is to culture the peripheral blood mononuclear fraction in supplemented endothelial growth medium, removing the non-adherent cells, and isolating the remaining. (wikipedia.org)
  • ECFCs are isolated by plating peripheral blood mononuclear fraction on collagen-coated plates, removing non-adherent cells, and culturing for weeks until the emergence of colonies with a distinctive cobblestone morphology. (wikipedia.org)
  • If the potential donor meets all requirements, the NMDP collects their bone marrow or peripheral blood stem cells and sends them by courier to the patient. (wikipedia.org)
  • The hope is that stem cells transplanted into an injured area of the spinal cord will allow neuroregeneration. (wikipedia.org)
  • More recent findings suggest that pluripotent stem cells may reside in blood and adult tissues in a dormant state. (wikipedia.org)
  • The fully patent umbilical artery has two main layers: an outer layer consisting of circularly arranged smooth muscle cells and an inner layer which shows rather irregularly and loosely arranged cells embedded in abundant ground substance staining metachromatic. (wikipedia.org)
  • These long chains of sugar carbohydrates occur within the cells that help build bone, cartilage, tendons, corneas, skin and connective tissue. (wikipedia.org)
  • Over time, these glycosaminoglycans collect in the cells, blood and connective tissues. (wikipedia.org)
  • CD34+ cells may be isolated from blood samples using immunomagnetic or immunofluorescent methods. (wikipedia.org)
  • Injection of CD34+ hematopoietic Stem Cells has been clinically applied to treat various diseases including Spinal Cord Injury, Liver Cirrhosis and Peripheral Vascular disease. (wikipedia.org)
  • Research has shown that CD34+ cells are relatively more in men than in women in the reproductive age among Spinal Cord Injury victims. (wikipedia.org)
  • Immature granulocytes and nucleated red cells in the peripheral blood. (wikipedia.org)
  • In 2005, the PCRF lab at Columbia University reported the critical differences in cord blood compared to adult peripheral blood regulatory T-cells that may account for the significantly different responses after cord blood transplantation. (wikipedia.org)
  • In 1924, Russian-born morphologist Alexander A. Maximow used extensive histological findings to identify a singular type of precursor cell within mesenchyme that develops into different types of blood cells. (wikipedia.org)
  • Scientists Ernest A. McCulloch and James E. Till first revealed the clonal nature of marrow cells in the 1960s. (wikipedia.org)
  • Subsequent experimentation revealed the plasticity of marrow cells and how their fate is determined by environmental cues. (wikipedia.org)
  • These bone marrow stem cells do not contribute to the formation of blood cells so do not express the hematopoietic stem cell marker CD34, and are sometimes referred to as bone marrow stromal stem cells. (wikipedia.org)
  • However MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
  • Australian company Cynata claims the ability to mass-produce MSCs from induced pluripotent stem cells obtained from blood cells. (wikipedia.org)
  • spinal cord i
  • Spinal cord injury research seeks new ways to cure or treat spinal cord injury in order to lessen the debilitating effects of the injury in the short or long term. (wikipedia.org)
  • There is no cure for SCI, and current treatments are mostly focused on spinal cord injury rehabilitation and management of the secondary effects of the condition. (wikipedia.org)
  • PLacental
  • In September 2012 Pluristem reported saving the life of a third bone marrow disease patient using its PLacental eXpanded cell treatment, again at Jerusalem's Hadassah Medical Center and again under the terms of compassionate use. (wikipedia.org)
  • vessels
  • The umbilical cord contains Wharton's jelly, a gelatinous substance made largely from mucopolysaccharides which protects the blood vessels inside. (wikipedia.org)
  • Occasionally, only two vessels (one vein and one artery) are present in the umbilical cord. (wikipedia.org)
  • In absence of external interventions, the umbilical cord occludes physiologically shortly after birth, explained both by a swelling and collapse of Wharton's jelly in response to a reduction in temperature and by vasoconstriction of the blood vessels by smooth muscle contraction. (wikipedia.org)
  • liver
  • Within the fetus, the umbilical vein continues towards the transverse fissure of the liver, where it splits into two. (wikipedia.org)
  • One of these branches joins with the hepatic portal vein (connecting to its left branch), which carries blood into the liver. (wikipedia.org)
  • The second branch (known as the ductus venosus) bypasses the liver and flows into the inferior vena cava, which carries blood towards the heart. (wikipedia.org)
  • Physical symptoms generally include coarse or rough facial features (including a flat nasal bridge, thick lips, and enlarged mouth and tongue), short stature with disproportionately short trunk (dwarfism), dysplasia (abnormal bone size and/or shape) and other skeletal irregularities, thickened skin, enlarged organs such as liver (hepatomegaly) or spleen (splenomegaly), hernias, and excessive body hair growth. (wikipedia.org)
  • tissue
  • Preimplantation genetic diagnosis (PGD) has been used to enable families to have a child that is a tissue match for an existing sick sibling in need of an allogeneic haematopoietic stem cell (HSC) transplantation. (bmj.com)
  • Epidural cooling saddles, surgically placed over acutely traumatized spinal cord tissue, have been used to evaluate potentially beneficial effects of localized hypothermia, with and without concomitant glucocorticoids. (wikipedia.org)
  • artery
  • Closure of the umbilical artery by vasoconstriction consists of multiple constrictions which increase in number and degree with time. (wikipedia.org)
  • The artery in cords of preterm infants contracts more to angiotensin II and arachidonic acid and is more sensitive to oxytocin than in term ones. (wikipedia.org)
  • lymphoma
  • When your child has leukemia, lymphoma or a serious blood disorder, you're naturally worried and searching for the right care, with people who understand kids who are sick. (spectrumhealth.org)
  • This targeted therapy is especially effective to help your child fight lymphoma and other blood cancers. (spectrumhealth.org)
  • Guided Bone Regener
  • Guided Bone Regeneration in Standardized Calvarial Defects in Rats Using Bio-Oss and β-Tricalcium Phosphate with Adjunct Platelet-Derived Growth Factor Therapy: A Real-Time In Vivo Microcomputed Tomographic, Biomechanical, and Histologic Analysis. (semanticscholar.org)
  • vitro
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • tissues
  • These individuals do not have metachromatic deposits in peripheral nerve tissues, and their urine content of sulfatide is normal. (testcatalog.org)
  • Over time, these glycosaminoglycans collect in the cells, blood and connective tissues. (wikipedia.org)
  • These features may not be apparent at birth but progress as storage of glycosaminoglycans affects bone, skeletal structure, connective tissues, and organs. (wikipedia.org)
  • This results from compression of nerves or nerve roots in the spinal cord or in the peripheral nervous system, the part of the nervous system that connects the brain and spinal cord to sensory organs such as the eyes and to other organs, muscles, and tissues throughout the body. (wikipedia.org)
  • The baby is not harmed in any way by this collection, as the cord blood is collected from tissues that in the past had been discarded as medical waste. (wikipedia.org)
  • humans
  • The first attempt, an unsuccessful one, at gene therapy (as well as the first case of medical transfer of foreign genes into humans not counting organ transplantation) was performed by Martin Cline on 10 July 1980. (wikipedia.org)
  • vein
  • Bone marrow product is infused through a central vein over a period of several hours. (wikipedia.org)
  • Occasionally, only two vessels (one vein and one artery) are present in the umbilical cord. (wikipedia.org)
  • Within the fetus, the umbilical vein continues towards the transverse fissure of the liver, where it splits into two. (wikipedia.org)
  • One of these branches joins with the hepatic portal vein (connecting to its left branch), which carries blood into the liver. (wikipedia.org)
  • vascular
  • Peripheral vascular disease is a crucial health issue that affects an estimated 27 million people. (ahajournals.org)
  • When these findings are taken together, combination therapy with MNC transplantation and AM infusion may have additional or synergetic effects on therapeutic angiogenesis for the treatment of severe peripheral vascular disease. (ahajournals.org)
  • nervous
  • Metachromatic leukodystrophy (MLD) is a lysosomal storage disorder caused by a deficiency of the arylsulfatase A (ARSA) enzyme, which leads to the accumulation of galactosyl sulfatide (cerebroside sulfate) in the white matter of the central nervous system and in the peripheral nervous system. (testcatalog.org)
  • Clinical
  • Persons with mucolipidosis may share some of the clinical features associated with the mucopolysaccharidoses (certain facial features, bony structure abnormalities, and damage to the brain), and increased amounts of the enzymes needed to break down the lipids are found in the blood. (wikipedia.org)
  • However, aside from methylprednisolone, none of these developments have reached even limited use in the clinical care of human spinal cord injury in the US. (wikipedia.org)
  • cancers
  • Scientists have found a unique molecular signature in the blood, which can be used for the detection of aggressive lung cancers. (assuta.clinic)
  • treatment
  • Blood tests: A complete blood count (CBC) will be performed on a child suspected of having JMML and throughout the treatment and recovery of a child diagnosed with JMML. (wikipedia.org)
  • spinal cord
  • Spinal cord injury research seeks new ways to cure or treat spinal cord injury in order to lessen the debilitating effects of the injury in the short or long term. (wikipedia.org)
  • There is no cure for SCI, and current treatments are mostly focused on spinal cord injury rehabilitation and management of the secondary effects of the condition. (wikipedia.org)
  • Special devices exist to deliver blows of specific, monitored force to the spinal cord of an experimental animal. (wikipedia.org)
  • Surgery is currently used to provide stability to the injured spinal column or to relieve pressure from the spinal cord. (wikipedia.org)
  • Neuroregeneration aims to reconnect the broken circuits in the spinal cord to allow function to return. (wikipedia.org)
  • infusion
  • Thus, the purposes of the present study were (1) to investigate whether local infusion of AM enhances the angiogenic potency of MNC transplantation in a rat model of hindlimb ischemia and (2) to investigate the effects of AM on the survival, adhesion, and differentiation of transplanted MNCs. (ahajournals.org)
  • Transplantation of bone marrow-derived MNCs and infusion of AM were performed in 40 rats immediately after hindlimb ischemia was created. (ahajournals.org)
  • This protocol resulted in the creation of 4 groups: (1) AM infusion plus MNC transplantation (AM+MNC group, n=10), (2) AM infusion plus PBS injection (AM group, n=10), (3) vehicle infusion plus MNC transplantation (MNC group, n=10), and (4) vehicle infusion plus PBS injection (control group, n=10). (ahajournals.org)
  • donation
  • We, with the support of local NGO (Udham emergency blood donation) we did the drive at 9 locations nearby native place, from 25th May 6th June'17. (change.org)
  • organs
  • Physical symptoms generally include coarse or rough facial features (including a flat nasal bridge, thick lips, and enlarged mouth and tongue), short stature with disproportionately short trunk (dwarfism), dysplasia (abnormal bone size and/or shape) and other skeletal irregularities, thickened skin, enlarged organs such as liver (hepatomegaly) or spleen (splenomegaly), hernias, and excessive body hair growth. (wikipedia.org)