• Methods We first detected plasma catecholamine concentrations in patients with SLE and healthy controls by mass spectrometry, followed by reverse transcription-quantitative PCR (RT-qPCR) to detect DRD messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMCs) and CD4 + T cells, and flow cytometry to detect DRD expression in Tfh cells. (bmj.com)
  • Results The plasma dopamine concentration in patients with SLE was significantly increased, and abnormal mRNA expression of DRDs was observed in both PBMCs and CD4 + T cells. (bmj.com)
  • Recovered peripheral blood mononuclear cells (PBMCs) are stimulated for 6 to 16 hours with a viral antigen [77, 113-116]. (bestpfe.com)
  • However, the cytokine production was not affected when PBMCs were stimulated with lipopolysaccharide (LPS) and anti-CD28 monoclonal antibody which is a Ca2 +-independent cell activation process [132]. (bestpfe.com)
  • For example, human peripheral blood mononuclear cells (PBMCs) are often used for studying immunological functions like cellular surface marker expression and cytokine production. (akadeum.com)
  • AcceGen offers Human Peripheral Blood Pan Monocytes (Untouched) isolated from normal human peripheral blood mononuclear cells (PBMCs) using negative immunomagnetic selection (Kopelsonclinic). (accegen.com)
  • To investigate the relationship between CD8 activation and infection by HIV-1 in vivo, activated subsets of CD8 lymphocytes in peripheral blood mononuclear cells (PBMCs) of HIV-seropositive individuals were investigated for CD4 expression and HIV infection. (ox.ac.uk)
  • B cells are isolated from PBMC by positive immunomagnetic isolation using CD19 microbeads or negative selection through depletion of other cell types. (bloodworksbio.org)
  • Andersson et al tested the effects of FK506 and CsA on cytokine production of peripheral blood mononuclear cell (PBMC) after stimulation with phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore (ionomycin) [131]. (bestpfe.com)
  • Cells are generally isolated from PBMC from leukopaks. (bloodworksbio.org)
  • CD19+ B Cells are isolated from peripheral blood mononuclear cells by positive CD19 selection or by depletion of other cell types. (bloodworksbio.org)
  • Flow data comes with a label check instead of anti-CD19 antibody for positively selected cells due to antigen saturation during positive selection. (bloodworksbio.org)
  • After RBC depletion, a cell sample is ready to be separated into specific cell subsets or otherwise undergo further processing. (akadeum.com)
  • For additional B Cell subsets, such as Naïve B Cells, CD138+ B Cells, or Memory B Cells, please inquire here . (bloodworksbio.org)
  • HIV-1 may infect thymic precursor cells destined to become CD4 and CD8 lymphocytes and contribute to the numerical decline in both subsets on disease progression. (ox.ac.uk)
  • EA also enhanced the cytotoxicity of both fludarabine and cyclophosphamide against CLL cells in vitro. (oncotarget.com)
  • They reported that antigen specific proliferation of T cells and cytotoxicity of HLA-A1 antigen specific CTL line were only inhibited by CP and MTX among all the tested drugs. (bestpfe.com)
  • These cells are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity. (cytologicsbio.com)
  • We determined the capacity of the leukemia cell line K562 modified to express a membrane-bound form of interleukin (IL)-15 and 41BB ligand (K562-mb15-41BBL) to generate human NK cells with enhanced cytotoxicity. (aacrjournals.org)
  • These cells are subsequently processed using the Cytokine Capture System (CCS, Miltenyi Biotec) based on an IFN-γ immunomagnetic technology on the CliniMACS device (Miltenyi Biotec), as previously described [117, 118]. (bestpfe.com)
  • Endogenous cytokine levels after immunoselected CD34+ peripheral blood progenitor cell transplantation. (ntu.ac.uk)
  • While no clinical data establishes causal link between HIV acquisition and use of this injectable progestin, prior work from our laboratory showed that DMPA comparably lowers genital levels of the cell-cell adhesion molecule desmoglein-1 (DSG1) and weakens genital epithelial barrier function in female mice and humans. (stanford.edu)
  • Finally, in vitro experiments and RNA sequencing (RNA-seq) were used to explore the possible pathway by which DRDs regulate Tfh cell differentiation. (bmj.com)
  • In vitro induction experiments showed that differentiation of naïve T cells into Tfh cells was accompanied by an increase in D1-like receptor expression. (bmj.com)
  • The changes of immune cells and cytokines in vitro and in vivo were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • In vivo and vitro, Niraparib can also increase the proportion of T cells and combined with PD-L1 blockade could further enhance the effect. (biomedcentral.com)
  • The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
  • Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
  • Separation of chemotherapy plus G-CSF-mobilized peripheral blood mononuclear cells by counterflow centrifugal elutriation: in vitro characterization of two different CD34+ cell populations. (ntu.ac.uk)
  • The critical characteristic is their multipotentiality, that is generally established selleck chemical in vitro by retrospective analysis of their ability to differentiate into at the least three cell forms, normally adipocytes, osteocytes and chondrocytes, and these potencies could vary amongst mouse strains. (plasignaling.com)
  • rhG-CSF in healthy donors: mobilization of peripheral hemopoietic progenitors and effect on peripheral blood leukocytes. (ntu.ac.uk)
  • To determine if a specific subpopulation of CB-MNC are responsible for these protective activities, we depleted CB-MNC of various cell types and found that only removal of CB CD14 + monocytes abolished neuroprotection. (biorxiv.org)
  • These findings suggest that CD14 + monocytes are a critical cell-type when treating HI with CB-MNC. (biorxiv.org)
  • Peripheral blood CD14+ monocytes originate in the bone marrow and move via the bloodstream to infected or damaged peripheral tissue. (cytologicsbio.com)
  • Human peripheral blood CD14+ monocytes are isolated from the mononuclear cell fraction by means of negative selection. (cytologicsbio.com)
  • Our CD14+ monocytes are collected at FDA-licensed donor centers and further processed at our cell isolation laboratory. (cytologicsbio.com)
  • T lymphocytes were analyzed for the expression of autophagy-specific markers by biochemical, molecular, and histological assays in peripheral blood and labial gland biopsies. (biomedcentral.com)
  • Although the aberrant activation of the T cell response observed in pSS has partially been explained by the interaction of T lymphocytes both with the ductal epithelium and the infiltrating B cells via costimulatory molecules (i.e. (biomedcentral.com)
  • Chronic lymphocytic leukemia (CLL) is a B-cell hematological malignancy characterized by the clonal expansion and accumulation of morphologically mature B-lymphocytes in peripheral blood, bone marrow, and secondary lymphoid tissues. (oncotarget.com)
  • Activated peripheral CD8 lymphocytes express CD4 in vivo and are targets for infection by human immunodeficiency virus type 1. (ox.ac.uk)
  • CD4(+) and CD4(-) CD8 lymphocytes, CD4 lymphocytes, other T cells, and non-T cells were purified using paramagnetic beads, and proviral sequences were quantified by PCR using primers from the long terminal repeat region. (ox.ac.uk)
  • Frequencies of activated CD8 lymphocytes were higher in HIV-infected study subjects than in seronegative controls, and they frequently coexpressed CD4 (mean frequencies on CD69(+), CD71(+), and HLA class II(+) cells of 23, 37, and 8%, respectively, compared with 1 to 2% for nonactivated CD8 lymphocytes). (ox.ac.uk)
  • Our research goal was to reveal peculiarities of effects produced by this pathogen on γ-interferon as one of basic products by 1st type T-helpers and on contents of the 17th type T-helpers determined as cells belonging to CD4+CD161+ and CD4+IL17+ phenotypes under direct contacts between bacteria and lymphocytes. (fcrisk.ru)
  • We extracted lymphocytes with immunomagnetic separation out of mononuclear blood cells obtained via functioning in density gradient. (fcrisk.ru)
  • To our knowledge, three investigators[76, 106-108] reported the treatment of post-HSCT CMV infection using adoptive transfer of fresh CMV-specific CD8+ T cells generated by an immunomagnetic strategy based on HLA-peptide multimers from HSCT donors or third-party haploidentical donors. (bestpfe.com)
  • CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
  • They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
  • RhG-CSF-mobilized peripheral blood haemopoietic progenitors reside in G0/G1 phase of cell cycle independently of the expression of myeloid antigens. (ntu.ac.uk)
  • In clinical patient samples, Niraparib augmented cytotoxic CD8 + T cell proportion and function. (biomedcentral.com)
  • Cord blood (CB) mononuclear cells (MNC) are being tested in clinical trials to treat hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
  • Determining which cell types in CB-MNC enhance brain tissue repair, and the mechanisms by which they do so, will optimize decisions on dosing, route of administration, treatment frequency, and other critical clinical and regulatory parameters. (biorxiv.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • Many culturing strategies are based on the addition of feeder or accessory cells, which need to be removed prior to the clinical application of the final NK cell product. (frontiersin.org)
  • We therefore adapted the K562-mb15-41BBL stimulation method to large-scale clinical-grade conditions, generating large numbers of highly cytotoxic NK cells. (aacrjournals.org)
  • The results that we report here provide rationale and practical platform for clinical testing of expanded and activated NK cells for cell therapy of cancer. (aacrjournals.org)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
  • Our research objects were clinical isolates of Helicobacter pylori and blood samples taken from people without helicobacter infection in their case history. (fcrisk.ru)
  • The degree to which such cells or genuine MSCs may be utilized in tissue restore, gene or cancer therapy may have a bearing on lots of clinical outcomes. (plasignaling.com)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
  • The mRNA expression of PLEKHA4 was detected by PCR in glioma samples and controls and in glioma cells treated with lactic acid and glucose. (bvsalud.org)
  • Primary human CD19+ naïve B cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative immunomagnetic separation techniques. (stemcell.com)
  • As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. (cdc.gov)
  • We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. (cdc.gov)
  • We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. (cdc.gov)
  • It is critical to isolate SARS-CoV-2 viruses to characterize their ability to infect and replicate in multiple human cell types and to determine if the virus is evolving in its ability to infect human cells and cause severe disease. (cdc.gov)
  • In addition, we studied the replication kinetics of SARS-CoV-2/SB3-TYAGNC in human fibroblast, epithelial, and immune cells. (cdc.gov)
  • ATCC) were cultured as previously mentioned ( 4 ), as were THF cells (human telomerase life-extended cells) ( 5 ). (cdc.gov)
  • Programmed death ligand 1 (PD-L1) expression in human ovarian cancer cells after PARP inhibitors treatment was examined by western blotting (WB) and flow cytometry. (biomedcentral.com)
  • Strauss et al analyzed seven commonly used immunosuppressive drugs (dexamethasone (DEX), mycophenolic acid (MPA), FK506, CsA, rapamycin (RAP), methotrexate (MTX) and cyclophosphamide (CP)) for apoptosis-induction and effector function in human peripheral T cells and cytotoxic T cell lines (CTL) [133]. (bestpfe.com)
  • Mononuclear cell (MNC) prepared from human umbilical cord blood (CB) are candidate therapeutics for treating hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
  • It is best to use caution when handling any human cells. (cytologicsbio.com)
  • We recommend all human cells should be handled as if potentially infectious following biosafety level 2 procedures. (cytologicsbio.com)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • The work presented in this study carries animal modeling one step further by simultaneously humanizing the immune system of recipient NOD/SCID/IL-2Rγnull (NSG) animals and challenging them with implanted human tumor cells. (biomedcentral.com)
  • Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • Indeed, antigen-presenting cells are important reservoirs of HIV-1 [ 16,17 ] and induction of HIV-1 replication within these cells may contribute significantly to the cofactor effect of confections on HIV-1 pathogenesis. (lww.com)
  • Rosenplänter C., Sommer F., Kleemann P., Belkovets A., Schmidt A., Lohoff M. Helicobacter pylori polyclonally ac-tivates murine CD4+ T-cells in the absence of antigen-presenting cells. (fcrisk.ru)
  • However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
  • LTα knockout mice used here were designed to avoid the artifact involving TNF gene downregulation in myeloid cells, which occurred in the conventional LTα knockout mice. (rusimmun.ru)
  • The results of flow cytometry showed that D1-like receptors were highly expressed in Tfh cells of patients with SLE and associated with disease activity. (bmj.com)
  • Each product is characterized by flow cytometry to ensure a highly pure and viable cell population. (cytologicsbio.com)
  • The colony-forming ability of the cells was evaluated using plate cloning assay, and cell cycle changes and cell apoptosis were analyzed with flow cytometry. (bvsalud.org)
  • These TIL exhibited a unique phenotype when compared to splenocytes with a predominance of CD8+ T cells that exhibited increased expression of CD69, CD56, and an effector memory phenotype. (biomedcentral.com)
  • The progressive accumulation of leukemic cells is mostly ascribed to extended cellular survival rather than excessive cellular proliferation [ 1 ]. (oncotarget.com)
  • Interestingly, they found that DEX, MPA, CsA, FK506 and RAP only prevented activation of naive T cells, but did not block proliferation of activated T cells nor decrease cytotoxic capacity of CTL lines. (bestpfe.com)
  • A specific PLEKHA4 siRNA was transfected in glioma U251 and T98G cells, and the changes in cell proliferation ability were assessed by real-time cell analysis technology and Edu experiment. (bvsalud.org)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • It's of curiosity to determine that MSCs also express cell surface epidermal development issue receptor one and reply to the ligand heparin binding epidermal growth element with dose dependent proliferation, which reversibly impairs their trilineage differentiation potential right up until the stimulus is removed. (plasignaling.com)
  • CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
  • In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
  • Therefore, elucidating the mechanism of Tfh cell differentiation will contribute to SLE treatment. (bmj.com)
  • Previous studies have found that DRDs can regulate differentiation of immune cells. (bmj.com)
  • RNA-seq and RT-qPCR results indicate that D1-like receptors might promote Tfh cell differentiation through the Phosphatidylinositol3-kinase (PI3K)/protein kinase B (AKT)/Forkhead box protein O1 (FOXO1)/Kruppel-like factor 2 (Klf2) pathway. (bmj.com)
  • D1-like receptors may promote Tfh cell differentiation through the PI3K/AKT/FOXO1/Klf2 pathway. (bmj.com)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • Moreover CD4+ T-cells are necessary to support in vivo expansion and survival of CD8+ T cells [75, 112] to maintain virus-specific immune response following adoptive transfer for a long time. (bestpfe.com)
  • Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
  • In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
  • Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
  • If there is a preference for cells isolated from whole blood, cord blood, or apheresis, please list in order notes. (bloodworksbio.org)
  • We also compared adult and cord blood (CB) DCs to find a possible explanation for the age-dependent severity of SARS. (biomedcentral.com)
  • In the current study, we focused on the receptor responses in SARS-CoV infected DCs and compared adult and cord blood (CB) DCs to establish possible explanation for the age dependent severity of SARS. (biomedcentral.com)
  • Immunomagnetic HIV-1 capture analysis was used to determine the cellular origin of cell-free virus particles present in all 30 plasma samples and indices of immune activation were measured using enzyme-linked immunosorbent assays. (lww.com)
  • Parasite clearance following treatment with antimalarial drugs resulted in decreased detection of HIV-1 particles derived from the CD14 macrophage cell subset and correlated with a marked diminution in systemic immune activation. (lww.com)
  • As a critical part of sample preparation, RBC depletion is intended to clean up red blood cell contamination in biological samples, such as in bone marrow or dissociated tissue samples. (akadeum.com)
  • It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • An incredibly current finding may perhaps even cast doubt over the utility of MSCs themselves, Medici and colleagues reported that endothelial cells taken care of with either bone morphogenetic protein four or transforming development factor b2 reverted to a multipotent cell with some traits of MSCs, and can be vary entiated into several endodermal cell styles. (plasignaling.com)
  • Our buoyancy-activated technology is an innovative alternative to traditional blood separation techniques , significantly reducing costs, saving time, and simplifying workflows-all while improving the quality and viability of sorted cells. (akadeum.com)
  • Primary Sjögren's syndrome (pSS) is a common chronic autoimmune disease characterized by lymphocytic infiltration of exocrine glands and peripheral lymphocyte perturbation. (biomedcentral.com)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • As a key transcription factor of the Wnt/β-catenin pathway, LEF1 helps to regulate important genes involved in tumor cell death mechanisms. (oncotarget.com)
  • Immune reconstitution slowed but could not eliminate tumor growth and this effect required the presence of CD4+ T cell help. (biomedcentral.com)
  • New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
  • The field of circulating tumor cell (CTC) enrichment has seen many emerging technologies in recent years, which have resulted in the identification and monitoring of clinically relevant, CTC-based biomarkers that can be analyzed routinely without invasive procedures. (oaepublish.com)
  • The use of circulating tumor cells (CTCs) as a "liquid biopsy" to interrogate the molecular profile of single patients is extremely encouraging, as these cells originate from primary tumor and metastatic sites, providing a comprehensive fingerprint of a given tumor at any time during the course of the disease with a simple blood draw [ 7 ] . (oaepublish.com)
  • Besides, Niraparib activated the cGAS-STING pathway, increasing the levels of cytokines such as CCL5 and CXCL10, which played a vital role in augmenting the infiltration and activation of cytotoxic T cells. (biomedcentral.com)
  • Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
  • A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. (frontiersin.org)
  • The development of clinically applicable methods to produce large numbers of fully functional NK cells is a critical step to maximize the potential of this approach. (aacrjournals.org)
  • With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
  • To explore these mechanisms further, we exposed mouse brain organotypic slice cultures to oxygen and glucose deprivation (OGD) and then treated the brain slices with cells from CB or adult peripheral blood (PB). (biorxiv.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • Adult stem cells remain of particular interest as therapeutic agents for cardiac regeneration in ischemic heart disease [ 1 - 4 ]. (biomedcentral.com)
  • In this study, we show that compared with mice in estrus, treatment of mice with DMPA or NET-EN significantly decreased genital levels of the cell-cell adhesion molecule desmoglein-1 and increased genital mucosal permeability. (stanford.edu)
  • CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • By making cells float that would otherwise sink, microbubbles allow targeted cells to be isolated to a high level of purity. (akadeum.com)
  • Niraparib upregulated membrane PD-L1 and total PD-L1 expression in ovarian cancer cells and had a synergistic effect with PD-L1 blockade in vivo. (biomedcentral.com)
  • Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
  • In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
  • In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
  • Several researchers have applied single cell clones as a source of trilineage potent MSCs, whereas many others have shown their potential by in vivo analysis in xenografts. (plasignaling.com)
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
  • In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
  • Without limitation, some embodiments comprise methods, systems, and/or compositions relating to microRNAs and/or cell based therapies and the use of the same in the research, diagnosis, or treatment of injury or disease. (justia.com)
  • The basic conclusion is that MSCs or their closely relevant cousins can reside in or pass via most tissues, and that this kind of cells may be isolated and/or selelck kinase inhibitor cultured by standard methods this kind of as fluorescence activated cell sorting or plastic adherence, and will be shown to possess multipotency. (plasignaling.com)
  • As briefly illustrated in Tables one and two, there are many combina tions of cell surface markers that can be employed to pick MSCs from mixtures of cells, that are often performed by cell sorting using FACS or immunomagnetic procedures, although several scientific studies use plastic adher ent stromal cells that are subsequently classified using immunofluorescent phenotyping or movement cytometry. (plasignaling.com)
  • Interestingly, Niraparib enhanced the proportion and function of T cells via activation of the innate immune response pathway. (biomedcentral.com)
  • CP and MTX also slightly increased activation-induced cell death (AICD) and CD95-sensitivity. (bestpfe.com)
  • We also used positively selected subpopulations of CB-MNC and PB-MNC in this assay and demonstrated that purified CB-CD14 + cells, but not CB-PB CD14 + cells, efficiently protected neuronal cells from death and reduced glial activation following OGD. (biorxiv.org)
  • For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
  • However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
  • Interestingly, the SARS-CoV infected DCs showed low expression of antiviral cytokines (IFN-α, IFN-β, IFN-γ and IL-12p40), moderate upregulation of proinflammatory cytokines (TNF-α and IL-6) but significant upregulation of inflammatory chemokines (macrophage inflammatory protein (MIP)-1α/CCL3, regulated upon activation, normal T cell expressed and secreted (RANTES)/CCL-5, interferon-inducible protein of 10 kD (IP-10)/CXCL10 and monocyte chemotactic protein (MCP)-1/CCL2. (biomedcentral.com)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
  • Akadeum microbubble products offer a new way to approach red blood cell depletion. (akadeum.com)
  • Akadeum's RBC depletion microbubbles are designed to clean up samples with significant red blood cell contamination. (akadeum.com)
  • What Is Red Blood Cell Depletion? (akadeum.com)
  • The process of depletion is when a single cell type, such as red blood cells, is removed from a biological sample. (akadeum.com)
  • What Is the Best Red Blood Cell Depletion Method? (akadeum.com)
  • When RBC depletion microbubbles are added to a biological sample, they engage RBCs and rapidly lift the unwanted cells to the top of any container, regardless of shape or size. (akadeum.com)
  • Red blood cell depletion microbubbles offer a gentler cell separation approach. (akadeum.com)
  • On average, 0.88 × 10 6 viable CD133 + cells with a mean log 10 depletion of 3.23 ± 0.19 of non-target cells were isolated. (biomedcentral.com)
  • The entire manufacturing process, the examination of cell number and viability of the final CP as well as the depletion of non-target cells were part of the validation process, which was performed in compliance with EU guidelines for GMP. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Thus, these cells are becom ing almost ubiquitous residents in many tissues and organs. (plasignaling.com)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • Multimers are most readily made with class I HLA and can only select CD8+ T cells but not CD4+ T-cell subset restricted by class II HLA [110]. (bestpfe.com)
  • Browse our Frequently Asked Questions (FAQs) on Primary Cells. (stemcell.com)
  • Furthermore, Wnt inhibitor ethacrynic acid (EA) induced both apoptosis and necroptosis in primary CLL cells. (oncotarget.com)
  • However, analogous effects of NET-EN treatment on antivirus immunity and host susceptibility to genital infection are much less explored. (stanford.edu)
  • Likewise, we detected comparable mortality rates in DMPA- and NET-EN-treated wild-type and humanized mice after intravaginal infection with HSV type 2 or cell-associated HIV type 1, respectively, but NET-EN treatment was associated with slower onset of HSV-induced genital pathology and lower burden of systemic HIV disease. (stanford.edu)
  • In some viral infections, for example ADV infection, immune response is mainly supported by CD4+T cells [77, 111]. (bestpfe.com)
  • A recent study reported that oral antibiotic treatment caused dysbiosis of the vaginal microbiota, resulting in increased IL-33 secretion, leading to impaired antiviral T cell responses and enhanced susceptibility to lethal HSV2 infection 23 . (nature.com)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • MicroRNA-126 (miR-126) is endothelial cell (EC) specific and plays a key role in regulating EC function, controlling angiogenesis, and maintaining vascular integrity. (justia.com)
  • T follicular helper (Tfh) cells play a critical role in the pathological process of SLE. (bmj.com)
  • However, Mo-DC differ in their molecular phenotype and function from blood DC (BDC). (nih.gov)
  • OBJECTIVE@#To investigate the effect of lactic acid-induced upregulation of PLEKHA4 expression on biological behaviors of glioma cells and the possible molecular mechanism. (bvsalud.org)
  • Several molecular platforms have been used to investigate the molecular profile of the disease, from high throughput gene expression analyses down to single cell biological dissection. (oaepublish.com)
  • The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
  • CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
  • Microbubbles have no negative effect on cell viability. (akadeum.com)
  • In our study, we investigated the dynamic changes and responses of immune cells and cytokines during Niraparib treatment. (biomedcentral.com)
  • Our results showed that Niraparib could upregulate PD-L1 expression, but did not impair the function of immune cells, especially CD8 + T cells. (biomedcentral.com)
  • Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis. (thermofisher.com)
  • In this study, we determined LEF1 gene expression levels in CLL ( n = 197) and monoclonal B-cell lymphocytosis (MBL) ( n = 6) patients through real-time RT-PCR. (oncotarget.com)
  • Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)