Peripheral blood mononuclear cells cell surface display techniques. Medical search. Frequent questions

The HUCMSCs derived multiple donors were performed comprehensively analysis and potent assays including expressions of surface markers, viability, growth curve, karyotype analysis, tumorigenicity, differentiation potentials, and immune regulation capability. (researchsquare.com)
The HUCMSCs derived multiple donors kept a high consistency in surface marker expressions, viability, growth curve, tumorigenicity in nude mic, but had robust heterogeneities in differentiation potentials and immune regulations. (researchsquare.com)
Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
Here we sought to further understand the effects of idelalisib and duvelisib in vitro, and demonstrate that both idelalisib and duvelisib can inhibit T cell proliferation as well as Th1 and Treg differentiation in vitro, while promoting Th2 and Th17 differentiation. (nature.com)
We further demonstrate directly using intracellular flow cytometry that autoimmune toxicity in patients is associated with higher absolute numbers of CD4 and CD8 T cells with Th17 differentiation in peripheral blood prior to therapy, and that gastrointestinal tissues from patients with active autoimmune complications of PI3Kδ inhibitors show infiltration with Th17 + T cells. (nature.com)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. (cancerindex.org)
DC are professional antigen-presenting cells with the unique capacity to polarize the differentiation of T cells, thereby regulating the balance between inflammation and tolerance [ 12 ]. (hindawi.com)
CD14+ monocytes from IBD patients had significantly decreased cleaved H3T22, suggesting they were epigenetically primed for macrophage differentiation.We describe the first systems-level quantification of histone modifications across immune cells from IBD patients at a single-cell resolution revealing the increased epigenetic heterogeneity that is not possible with traditional ChIP-seq profiling. (stanford.edu)
BAFF and APRIL also promote plasma cell survival by engagement of BCMA, a receptor expressed during the latest B cell differentiation stages [8], [9]. (bso14.org)
Different studies statement that cells in the late G2 phase undergo the process Calcitriol (Rocaltrol) of differentiation into cysts when faced with harsh environmental conditions [60,61,62,63]. (globaltechbiz.com)
It is interesting to study the initiation and regulation of differentiation in cells having no G1 phase, Mouse monoclonal to Ractopamine as typically, cell differentiation occurs from your G1 phase of the cell cycle. (globaltechbiz.com)
The basic and common immunologic defect in common variable immunodeficiency is a failure of B-lymphocyte differentiation into plasma cells that produce the various immunoglobulin (Ig) isotypes. (medscape.com)
These studies described failure of B-cell differentiation because of altered B-cell surface-molecule expression. (medscape.com)

for example, a particular drug might inhibit kinase activity in T cells but not in monocytes. (genengnews.com)
Using a chemo-genetic approach, we identify BET bromodomain containing proteins BRD2 and BRD4 as central regulators of NK cell functions, including direct cytokine secretion, NK cell contact-dependent inflammatory cytokine secretion from monocytes as well as NK cell cytolytic functions. (frontiersin.org)
The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)
To determine if a specific subpopulation of CB-MNC are responsible for these protective activities, we depleted CB-MNC of various cell types and found that only removal of CB CD14 + monocytes abolished neuroprotection. (biorxiv.org)
These findings suggest that CD14 + monocytes are a critical cell-type when treating HI with CB-MNC. (biorxiv.org)
Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)

Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
The signature was also present across T cell subsets and clonotypes, indicating that persisting CAR-T cells converge transcriptionally. (nature.com)
The capability of supervised classification algorithms in CytoPy to identify cell subsets was successfully confirmed by using the FlowCAP-I competition data. (biorxiv.org)
C, D. Same coordinates as in A and B, but only T-cell subsets are displayed. (bdbiosciences.com)
In human blood, two major subsets of DC have been characterized, that is, myeloid or conventional DC (cDC) and plasmacytoid DC (pDC) [ 13 ]. (hindawi.com)
It is of great significance to gain insight into the classification of γδ T cell subsets. (explorationpub.com)

Examination of single T cell transcriptomes from a wide range of healthy and diseased tissues across children and adults indicated that the persistence signature may be specific to long-lived CAR-T cells. (nature.com)

For example, the authors have found that AM from young children are less suppressive for T-lymphocyte proliferation using in vitro functional assays 2 , but the developmental changes of relevant immune receptors could not be identified because of the limited numbers of cells recovered. (ersjournals.com)
Routine assays to assess immunomodulation activity examine MSC effects on proliferation of peripheral blood mononuclear cells (PBMCs) and take 3-7 days. (bvsalud.org)
MSCs suppress peripheral blood mononuclear cell (PBMC) proliferation in vitro, suggesting a mechanism for suppressing inflammatory responses in vivo. (bvsalud.org)
CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand. (biolegend.com)
The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. (biomedcentral.com)
It was revealed that the M. tuberculosis infected DC induced T cell proliferation. (biomedcentral.com)
These data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro . (biomedcentral.com)

Automation of the entire process-from sample preparation, to running complex assays, to data analysis-makes it possible to perform functional phenotypic profiling of cell populations efficiently. (genengnews.com)
He described the graphical interface the group developed-a logic map designed to analyze the data from functional live-cell assays that incorporate fluorescent markers. (genengnews.com)
We reasoned that single-cell transcriptomic assays may help elucidate these features. (nature.com)
However, in whole blood, opsonophagocytic and intracellular killing assays, the LH/HC phenotype demonstrated higher resistance to host phagocytic killing. (cdc.gov)
Immunoblotting, reporter assays, quantitative RT-PCR, and chromatin immunoprecipitation were performed to evaluate HIF-1 signaling in glioblastoma cells. (cancerindex.org)

To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
MSCs have also been derived from embryonic tissues, such as Wharton's jelly and umbilical cord blood [ 13 , 14 ]. (hindawi.com)
Clinically, circulating EPCs can be obtained from adult peripheral blood and umbilical cord blood. (biomedcentral.com)
Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
Mononuclear cell (MNC) prepared from human umbilical cord blood (CB) are candidate therapeutics for treating hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
the b95-8 strain of epstein-barr virus (ebv) induced colony formation of human umbilical cord-blood leukocytes in soft agar medium. (liverpool.ac.uk)

In children, the description of developmental changes in immune receptor expression on alveolar macrophages (AM) has been limited by the small number of cells recovered by bronchoalveolar lavage (BAL). (ersjournals.com)
It is concluded that laser scanning cytometry is suited to the study of immune receptor expression from small numbers of paediatric alveolar macrophages, when CD68 is used for cell identification. (ersjournals.com)
Mice lacking PI3Kγ show increased CD8 T cell activation and cytotoxicity due to transcriptional activation of NFκB and inhibition of C/EBPβ in macrophages within the microenvironment [ 9 ]. (nature.com)
IL1α was predominantly seen in vascular endothelial cells, whereas IL1β staining was mainly shown in macrophages and fibroblasts. (bmj.com)
In today's study, we looked into the immunological function of Kupffer cells/macrophages in CCl4-induced hepatitis in mice. (enmd-2076.com)
The populace of Compact disc11b+ Kupffer cells/macrophages significantly elevated 24 hour (h) after CCl4 administration, in c-lipo-pretreated mice especially. (enmd-2076.com)
Oddly enough, the serum MCP-1 Metoclopramide HCl level elevated and peaked at six h after c-lipo pretreatment quickly, suggesting which the MCP-1 made by c-lipo-phagocytized Compact disc68+ Kupffer cells Metoclopramide HCl may recruit Compact disc11b+ macrophages in the periphery and bone tissue marrow. (enmd-2076.com)

The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
These MSCs derived different tissues do not have uniform characteristics, differing in expression profiles of surface markers and biological functions after certain stimulation such as pro-inflammatory mediators [5, 13]. (researchsquare.com)
Over the past few years, MSCs researches have achieved some inspiring results and some of which moved up to clinic period from preclinical phases, resulting in the marketing approval of a few cell-based therapy products (CTPs) by different national regulatory authorities [18]. (researchsquare.com)
Mesenchymal stromal cells (MSCs) are at the forefront of the cellular therapy revolution with profound immunomodulatory, regenerative, and therapeutic potential. (bvsalud.org)
BACKGROUND: Immunomodulation by mesenchymal stromal cells (MSCs) is a potentially important therapeutic modality. (bvsalud.org)
Cell-based therapy utilizing mesenchymal stromal cells (MSCs) is an attractive option for augmenting the fracture repair process. (hindawi.com)
Osteoprogenitor MSCs not only differentiate into bone, but they also exert modulatory effects on immune cells via a variety of mechanisms. (hindawi.com)
In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)
Multipotent mesenchymal stromal cells (MSCs), also known as mesenchymal stem cells, have the capacity to differentiate into a variety of cell types (Figure 1 ), including adipocytes, chondrocytes, and osteocytes [ 9 , 10 ]. (hindawi.com)
Coupled with reports that allogeneic MSCs have immunoprivileged status and immunomodulatory properties, there has been considerable interest in exploring the use of these cells as a therapeutic option for bone repair. (hindawi.com)
MSCs are able to evade the host cell immune system due to their low expression of major histocompatibility complex (MHC) class I molecules and complete lack MHC class II molecules and other costimulatory molecules (CD40, CD40L, CD80, and CD86) required for immune cell stimulation [ 15 - 17 ]. (hindawi.com)

In 2006 administration of normal circulating lymphocytes transduced with a retrovirus encoding a T-cell receptor (TCR) that recognized the MART-1 melanoma-melanocyte antigen, mediated tumor regression. (wikipedia.org)
In 2010 administration of lymphocytes genetically engineered to express a chimeric antibody receptor (CAR) against B cell antigen CD19 was shown to mediate regression of an advanced B cell lymphoma. (wikipedia.org)
In 2016, CD19-specific chimeric antigen receptor (CAR)-modified T cells were used to treat patients with relapsed and refractory CD19+ B cell malignancies, including B cell acute lymphoblastic leukemia (B-ALL) harboring rearrangement of the mixed lineage leukemia (MLL) gene with CD19 CAR-T cells. (wikipedia.org)
Laser scanning cytometry (LSC) generates quantitative information on immune receptor expression from cells cytocentrifuged onto a microscope slide. (ersjournals.com)
To obtain immune receptor data from the cell surface and cytoplasm after nuclear capture, a "contour" is generated at a fixed distance from the nuclear perimeter. (ersjournals.com)
In the context of relapsed and refractory childhood pre-B cell acute lymphoblastic leukemia (R/R B-ALL), CD19-targeting chimeric antigen receptor (CAR)-T cells often induce durable remissions, which requires the persistence of CAR-T cells. (nature.com)
In this study, we systematically analyzed CD19 CAR-T cells of 10 children with R/R B-ALL enrolled in the CARPALL trial via high-throughput single-cell gene expression and T cell receptor sequencing of infusion products and serial blood and bone marrow samples up to 5 years after infusion. (nature.com)
The advent of CD19 chimeric antigen receptor (CAR)-T cell therapy in recent years has transformed the treatment of intractable ALL 2 . (nature.com)
One mechanism of NK cell anti-tumor activity is antibody-dependent cellular cytotoxicity (ADCC) induced via Fc receptor (CD16) binding to antibodies. (maxcyte.com)
Ex vivo expanded NK cells electroporated with mRNA encoding the high-affinity Fc receptor, CD16-158V, demonstrated a significant increase in CD16 surface expression (Figure 1A). (maxcyte.com)
Ex vivo expanded NK cells were electroporated with mRNA (4 µg/10 6 NK cells) encoding the high-affinity, CD16-158V Fc receptor. (maxcyte.com)
γδ T cells express unique T cell receptor (TCR) γ and TCR δ chains, with structural and functional heterogeneity. (explorationpub.com)
Taking advantage of the diverse γδ TCR repertoire or other ligand-receptor interactions, γδ T cells can recognize a broad spectrum of tumor-associated antigens (TAAs) in a major histocompatibility complex (MHC)-independent manner, thereby activating downstream pleiotropic effects. (explorationpub.com)
Although these results do not formally rule out the existence of BAFF or APRIL reverse signalling (via pathways not tested in this study), they provide no evidence in support of reverse signalling and point to the importance of using appropriate specificity controls when working with Fc receptor-expressing myeloid cells. (bso14.org)
Interleukin-1 receptor-associated kinase 4 (IRAK4) was the dominant alternatively spliced isoform in MDS and AML and is characterized by a longer isoform that retains exon 4, which encodes IRAK4-long (IRAK4-L), a protein that assembles with the myddosome, results in maximal activation of nuclear factor kappa-light-chain-enhancer of B cells (NF-κB) and is essential for leukaemic cell function. (cancer-genetics.org)

In comparison to seven different antigens, using the specificity was verified with the ELISA technique of Nanobody 3hCTL55 against human CTLA-4 antigen. (radarcon2008.org)
Antigens present on cell surface membranes and extracellularly in the tissue specimens are generally accurately shown after acetone fixation, while the intracellular morphology is poorly preserved. (bmj.com)
Sixty percent of cases express 1 or more T-cell antigens (CD3 + , CD43, or CD45RO). (medscape.com)
MELOE-1 and MELOE-2, two highly specific melanoma antigens involved in T cell immunosurveillance are produced by IRES-dependent translation of the long « non coding » and polycistronic RNA, meloe . (oncotarget.com)
In conclusion, within the MELOE family, IRES-dependent antigens represent the best T cell targets for immunotherapy of melanoma. (oncotarget.com)
In this respect, the choice of the targeted antigens remains a critical issue [ 2 ] and the ideal antigen(s) should have the following characteristics : be as tumor specific as possible and stimulate a broad T cell repertoire in the majority of patients (i.e. generate many epitopes in various HLA contexts). (oncotarget.com)
Interestingly, the number of CD80 + pDC positively correlates with the presence of IL-10-producing regulatory type 1 cells (Tr1), an important cell type for maintaining peripheral tolerance to self-antigens. (hindawi.com)
To perform this function, DC are capable of capturing antigens, processing them, and presenting them on the cell surface complexed to major histocompatibility (MHC) molecules, for example, human leukocyte antigen- (HLA-) DR. Along with that, context-dependent expression of costimulatory molecules, such as CD80 and CD86, and secretion of cytokines occurs. (hindawi.com)
Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. (biomedcentral.com)

Laser scanning cytometry (LSC) is a new technique that potentially fulfils these requirements by combining the benefits of FC with those of IA and is built around the epi-fluorescence microscope 10 . (ersjournals.com)
Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)
Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)
NK cell CD16 expression was measured via flow cytometry for 72 hours post electroporation. (maxcyte.com)
Performing traditional western blot and Flow-cytometry methods demonstrated that Nanobody 3hCTL55 could specifically identify and connect both commercial individual CTLA-4 proteins and individual CTLA-4 antigen over the cell surface area and in the cell lysate. (radarcon2008.org)
PBMC surface marker expression was analyzed by flow cytometry. (bvsalud.org)
In response to this there has been an increased effort to develop computational methodologies for handling high-dimensional single cell data acquired by flow or mass cytometry. (biorxiv.org)
Despite the success of numerous algorithms and published packages to replicate and outperform traditional manual analysis, widespread adoption of these techniques has yet to be realised in the field of cytometry. (biorxiv.org)
To this end, a computational approach to analysis of cytometry data can take one of two strategies: to separate single cell data into groups or classifications, which then form the variables (often descriptive statistics of the obtained groups) the investigator uses to test their hypothesis, or directly model the acquired distribution of single cell data with respect to a chosen endpoint. (biorxiv.org)
Mass-cytometry-based quantitation of global histone post-translational modifications at single-cell resolution across peripheral immune cells in IBD. (stanford.edu)
To analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. (biomedcentral.com)
The immunohistochemical evaluation from the liver organ and the stream cytometry from the liver organ mononuclear cells demonstrated that clodronate liposome (c-lipo) treatment significantly reduced the spindle-shaped F4/80+ or Compact disc68+ cells, as the oval-shaped F4/80+ Compact disc11b+ cells elevated. (enmd-2076.com)

the colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. (liverpool.ac.uk)

In these tissues, the cells in the basal sur face carry on to divide even though these in the apical surface differentiate and cornify, forming a characteristic stratum corneum. (mirnaarray.com)
In the tissues that have been infected by the apical surface, GFP staining was uncovered within the cells close to the apical surface, suggesting that the apical cells have been infected with HCMV. (mirnaarray.com)

Henderson et al reported that human monocyte derived DC phagocytose M. tuberculosis efficiently, suggesting a role for this important cell in the early response to TB infection. (biomedcentral.com)

This persistence signature was also detected in two adult patients with chronic lymphocytic leukemia with decade-long remissions who received a different CD19 CAR-T cell product. (nature.com)
An exception was long-lived CAR-T cells in two adult individuals with a different cancer-chronic lymphocytic leukemia (CLL)-in whom anti-CD19 CAR-T cells have persisted for almost a decade thus far 7 . (nature.com)
It is unclear whether one can generalize from two adult patients treated for CLL to other hematological malignancies and patient groups, in particular to childhood ALL, or to other CAR-T cell products. (nature.com)
To explore these mechanisms further, we exposed mouse brain organotypic slice cultures to oxygen and glucose deprivation (OGD) and then treated the brain slices with cells from CB or adult peripheral blood (PB). (biorxiv.org)

In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)

We show that both BRD2 and BRD4 control inflammatory cytokine production in NK cells isolated from healthy volunteers and from rheumatoid arthritis patients. (frontiersin.org)
This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
Moreover, NK cells are poised to release cytokines such as IFN- γ , TNF-α and growth factors that can initiate inflammatory responses mediated by both the innate and the adaptive arm of the immune system. (frontiersin.org)
Many preclinical studies suggest that CB-MNC protect the brain after HI by releasing neurotrophic and anti-inflammatory factors that stimulate repair by host cells [ 8 , 9 ]. (biorxiv.org)
GBS production of β-h/c is encoded by the genes of the cyl operon ( 5 , 6 ) and is associated with direct lysis of a variety of eukaryotic cell types ( 7 - 9 ), inflammatory activation ( 10 - 12 ), and virulence in animal models ( 10 , 13 , 14 ). (cdc.gov)
Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g. (mdpi.com)
Based on previous efforts focusing on the role of the adaptive immune system in the pathogenesis of MS, it is currently well established that autoreactive T helper type 1 (Th1) and Th17 cells mediate the inflammatory processes in the CNS [ 10 ]. (hindawi.com)
Current understanding of histone post-translational modifications (histone modifications) across immune cell types in patients with inflammatory bowel disease (IBD) during remission and flare is limited. (stanford.edu)
Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRalpha+ Cells Is a New Marker for Inflammatory Bowel Disease. (stanford.edu)
Rare causes of specific inflammatory cardiomyopathies include cardiac sarcoidosis, giant cell myocarditis and eosinophilic myocarditis. (intechopen.com)

During tumor development, stromal cells are co-opted to the tumor milieu and provide favorable conditions for the tumor. (cancerindex.org)

Dendritic cell as sentinel of the immune system plays an irreplaceable role. (medsci.org)
We focus on the essential functions of dendritic cell in severe SARS-CoV-2 infection. (medsci.org)

Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
NK cells play a pivotal role in rapid and highly efficient cancer surveillance and represent a promising avenue for adoptive cell transfer either as a mono- or combination immunotherapy. (maxcyte.com)
The CD11b+ Kupffer cells producing TNF and FasL play a pivotal role in CCl4-induced acute hepatic injury thus. (enmd-2076.com)

Since recent evidence supports a crucial role of dendritic cells (DC) in the pathogenesis of MS, we investigated the effect of a 12-week combined exercise program in MS patients on the number and function of DC. (hindawi.com)
In particular, the role of Dendritic cells (DCs) in the initiation of specific T cell immunity to M. tuberculosis has not been clearly elucidated. (biomedcentral.com)

ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)

The precise spatial and temporal impact of immune cells and their cytokines on fracture healing remains obscure. (hindawi.com)
This clustered distribution was not found for cells expressing any of the other studied cytokines. (bmj.com)
Techniques based on immunohistochemistry have thus also been widely used to study the expression of cytokines within the synovial tissue in attempts to determine true local production. (bmj.com)
As a result, these liver organ immune system cells, including B cells and their cytokines, mainly become innate immune effectors against tumors and infections simply by their T helper-1 immune response in the liver organ. (enmd-2076.com)

Yang Y, Huang Z, Niu X, Xu H, Li Y, Liu W. Clinical characteristics and risk factors analysis of lung metastasis of benign giant cell tumor of bone. (medscape.com)
This method allows for multiplexed analysis of cell types and signaling pathways, and has applications in drug target identification and throughout the drug development workflow, including in the analysis of patient samples during clinical testing. (genengnews.com)
Previously, we generated a novel low-affinity CAR incorporating a CD19-specific single-chain variable fragment (scFv) called CAT, displaying a faster off-rate of interaction than the FMC63 CD19 binder used in prior clinical studies 3 . (nature.com)
PI3Kδ inhibitors are approved for the therapy of B cell malignancies, but their clinical use has been limited by unpredictable autoimmune toxicity, despite promising efficacy and evidence that toxicity is associated with improved clinical outcomes. (nature.com)
Early studies on the transfer of unmodified autologous or allogeneic NK cells have established their clinical safety and demonstrated modest anti-tumor efficacy. (maxcyte.com)
Patients homozygous for a natural CD16 polymorphism, CD16-158V which displays higher IgG1 and IgG3 affinity, have improved clinical outcomes upon anti-tumor therapeutic antibody treatment. (maxcyte.com)
Only 10% of the human population is homozygous for this polymorphism, suggesting that genetic manipulation of NK cells to express CD16-158V prior to adoptive transfer may improve clinical success not only of NK cell therapies but also anti-tumor IgG1 antibodies. (maxcyte.com)
Non-viral engineering of NK cells using MaxCyte ® mRNA electroporation provides significant benefits including high efficiency and low toxicity as well as clinical scalability enabling rapid development of novel adoptive cell therapy approaches. (maxcyte.com)
Cord blood (CB) mononuclear cells (MNC) are being tested in clinical trials to treat hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
Determining which cell types in CB-MNC enhance brain tissue repair, and the mechanisms by which they do so, will optimize decisions on dosing, route of administration, treatment frequency, and other critical clinical and regulatory parameters. (biorxiv.org)
However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)
positive serum tests for infectious mononucleosis (im) unaccompanied by the clinical syndrome or blood changes characteristic of the disease were detected in 39/177 (22%) mentally subnormal patients investigated with three different commercially available im slide tests. (liverpool.ac.uk)
clinical applications of cell surface markers. (liverpool.ac.uk)

Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)
Engineered NK cells had an enhanced ability to mediate ADCC as measured both via NK cell degranulation (CD107a expression, Figure 2A) and specific lysis of B cell lymphoma cells ( 51 Cr release assay, Figure 2B). (maxcyte.com)
However, what factors mediate brain repair, the CB-MNC cell types that contribute, and the host cells with which they interact are unclear. (biorxiv.org)
γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)

In 1986, human TILs from resected melanomas were found to contain cells that could recognize autologous tumors. (wikipedia.org)
His work covers various topics, including B cell responses to viral infection and vaccination, human primary immunodeficiency, and biology of lymphocyte development and function and to elucidate etiology of immunological disorders. (stanford.edu)
At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)
In this review, we use the Heilig nomenclature to describe human γδ T cells [ 1 ]. (explorationpub.com)
In the human monocyte cell line THP1, different anti-BAFF antibodies, but not a control mouse IgG antibody, induced, among others, phosphorylation of the mitogen-activated protein kinases ERK1/2, activation of the transcription factor NF-B, secretion of the matrix metallo-protease 9 (MMP9), secretion of the chemokine IL-8 and upregulation of the adhesion molecule ICAM-1 [12]. (bso14.org)
BAFF is important for supporting B cell survival also in human, and administration of atacicept in patients reduces B lymphocyte numbers and immunoglobulin levels [16], [17]. (bso14.org)
establishment of ebna-expressing cell lines by infection of epstein-barr virus (ebv)-genome-negative human lymphoma cells with different ebv strains. (liverpool.ac.uk)
cells of two ebna (epstein-barr virus nuclear antigen)-negative human lymphoma cell lines, bjab and ramos, were infected with two strains of epstein-barr virus (ebv). (liverpool.ac.uk)
Whether the encounter between the human dendritic cells and M. tuberculosis represents a defence mechanism by the invaded host, or helping the invader to evade the defence mechanism of the host is still not clearly understood. (biomedcentral.com)
It really is today generally accepted which the livers of mice and human beings contain types of innate immune system cells [11]C[13]. (enmd-2076.com)
In this study, we developed a technique to determine the LOD in terms of oocysts, using a cell sorter to sort 1 to 50 C. cayetanensis oocyst(s) previously purified from three human stool sources. (cdc.gov)

This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
The BD ® AbSeq Assay facilitates identification of specific changes in the myeloid cells of high-fat diet (HFD) mice. (bdbiosciences.com)
Expression of key cell surface markers identified by the BD ® AbSeq Assay in both healthy and CLL samples. (bdbiosciences.com)
We also used positively selected subpopulations of CB-MNC and PB-MNC in this assay and demonstrated that purified CB-CD14 + cells, but not CB-PB CD14 + cells, efficiently protected neuronal cells from death and reduced glial activation following OGD. (biorxiv.org)
NanoString nCounter assay Genomic DNA from fresh frozen tissue and cultured cell lines was extracted employing a DNA extraction kit following the makers professional tocol. (mirnaarray.com)
The adoptive transfer and cytotoxic assay against principal cultured hepatocytes verified the function of Compact disc11b+ Kupffer cells in CCl4-induced hepatitis. (enmd-2076.com)
Prevaccination and 1-month-postvaccination sera were tested by hemagglutination inhibition (HI) assay against 4 cell culture propagated vaccine reference viruses. (cdc.gov)

In autologous cancer immunotherapy, T cells are extracted from the patient, genetically modified and cultured in vitro and returned to the same patient. (wikipedia.org)
Description of T cell growth factor interleukin-2 (IL-2) in 1976 allowed T lymphocytes to be grown in vitro, often without loss of effector functions. (wikipedia.org)
CAT CAR-T cells showed greater cytotoxicity and proliferative responses in vitro and maintained long-lived molecular remissions in children with relapsed or refractory ALL, as demonstrated in the CARPALL study 3 . (nature.com)
Description: Agonist activity determined in vitro, measuring calcium response in CHO-K1 cells expressing hGPR15 and Aequorin. (guidetopharmacology.org)
Post electroporation, cells were resuspended in NK cell media and transferred to culture flasks for in vitro analysis. (maxcyte.com)
In vitro cytotoxic activity against rituximab-coated CD20+ B cell lymphoma cells of CD16-engineered or non-engineered NK cells was assessed 24 hours post electroporation. (maxcyte.com)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
Data from in vitro translation experiments and transfection of melanoma cells with bicistronic vectors documented that MELOE-3 is exclusively translated by the classical cap-dependent pathway. (oncotarget.com)
Furthermore, in vitro stimulation of PBMC from 6 healthy donors with overlapping peptides from MELOE-1 or MELOE-3 revealed a very scarce MELOE-3 specific T cell repertoire as compared to the abundant repertoire observed against MELOE-1. (oncotarget.com)

The use of circulating tumor cells (CTCs) as a "liquid biopsy" to interrogate the molecular profile of single patients is extremely encouraging, as these cells originate from primary tumor and metastatic sites, providing a comprehensive fingerprint of a given tumor at any time during the course of the disease with a simple blood draw [ 7 ] . (oaepublish.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)

In checkpoint therapy, antibodies bind to molecules involved in T-cell regulation to remove inhibitory pathways that block T-cell responses, known as immune checkpoint therapy. (wikipedia.org)
Using FC, fluorescent antibodies have attached to immune receptors on cells which are then identified as AM by their characteristic autofluorescence, light scatter properties ( e.g. forward scatter) or a combination of both 6 . (ersjournals.com)
Establish overexpression of high-affinity CD16 on NK cells as a potential combination therapy for improving ADCC activity of anti-tumor monoclonal antibodies. (maxcyte.com)
In the field of cancer immunotherapy, the recently described effectiveness of checkpoint inhibitors such as anti-PD1 blocking antibodies to boost anti-tumor T cell responses is very encouraging [ 1 ]. (oncotarget.com)
Similar observations were made in THP1 cells stimulated with anti-APRIL antibodies [13]. (bso14.org)

The fungal metabolite cyclosporin A (cyclosporine) [ 384 , 385 ] has a greater specificity for lymphoid T cells than other cells. (nanomedicine.com)
In due course, the stem cells in the bone marrow reconstitute the peripheral lymphoid system but the newly formed T cells seem to accept the graft as self [ 403 ]. (nanomedicine.com)
The detection of GPR15 in the colon suggests it may be important for transmission of HIV and SIV, and the authors speculate that the high expression in the colon suggests it may be present in non-lymphoid cells [ 11 ]. (guidetopharmacology.org)
Distinct lymphoid, myeloid, and stromal cell types in the TME exert opposing influences on PDAC tumor trajectory, suggesting a more complex organization than the classical "hot" versus "cold" tumor distinction. (stanford.edu)

We show that long-lived CAR-T cells developed a CD4/CD8 double-negative phenotype with an exhausted-like memory state and distinct transcriptional signature. (nature.com)
Specifically, demonstrate efficient expression of high-affinity CD16 in NK cells with minimal effects on cell viability and phenotype using mRNA electroporation and assess the effects of CD16-158V expression on rituximab - mediated cytotoxic activity against B cell lymphoma cells. (maxcyte.com)
Importantly, no major changes in NK cell phenotype, as measured by expression of 15 inhibitory or activating surface receptors, were observed following electroporation with CD16-158V mRNA. (maxcyte.com)
Hypoxia stimulates cancer cells to acquire a more malignant phenotype via activation of hypoxia-inducible factor 1 (HIF-1). (cancerindex.org)
These pDC display an activated phenotype, as evidenced by increased numbers of circulating CD62L + and CD80 + pDC. (hindawi.com)

Given that cancer cells and astrocytes in glioblastomas coexist in a hypoxic microenvironment, we examined whether astrocytes affect the adaptation of glioblastoma cells to hypoxia. (cancerindex.org)
Therefore, an in-depth understanding of γδ T cells that play conflicting roles in the tumor microenvironment is necessary. (explorationpub.com)

Oddly enough, liver organ B cells (mainly B-2 cells) generate IL-12 and IFN- however, not IgM, in response to LPS (vice versa for spleen B cells) [14]. (enmd-2076.com)
Furthermore, these IL-12-making liver organ B cells, as opposed to spleen B cells, phagocytose bacterias and eliminate them [15], [16]. (enmd-2076.com)

In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
Activated γδ T cells exhibit strong cytotoxic activity and cytokine secretion functions and are effective antitumor lymphocytes with simple and direct recognition modes and rapid responses. (explorationpub.com)
mice display Fangchinoline strongly impaired T cell-independent type II antibody responses, in line with data showing that TACI engagement is required for survival of B cells activated by T-independent type II stimuli [6], [7]. (bso14.org)
We compared antibody responses to high-dose egg-based inactivated (HD-IIV3), recombinant (RIV4), and cell culture-based (ccIIV4) vs standard-dose egg-based inactivated influenza vaccine (SD-IIV4) among health care personnel (HCP) aged 18-65 years in 2 influenza seasons (2018-2019, 2019-2020). (cdc.gov)

Adoptive cell transfer (ACT) is the transfer of cells into a patient. (wikipedia.org)

This capability is particularly important when studying heterogeneous cell populations and a variety of different phosphoproteins. (genengnews.com)
The researchers were able to correlate cell populations with pathway activity. (genengnews.com)
fluorescence immunomicroscopy detected association of PBMC with MSC and phosphatidyl serine externalization in both cell populations. (bvsalud.org)
As the field evolves, the traditional method of manual gating by sub-setting single cell data into populations and encircling data points in hand-drawn polygons in two-dimensional space has proven laborious, subjective, and difficult to standardise. (biorxiv.org)
Single-cell AbSeq and targeted mRNA-Seq analyses of myeloid cell populations localized in adipose tissue from control and HFD mice. (bdbiosciences.com)

t-SNE visualization of differences in expression of Adgre1 (encodes F4/80) gene expression and CD11c protein expression (identified via AbSeq) in different cell clusters of myeloid cells from the adipose tissue of HFD mouse. (bdbiosciences.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

The expression of chromatin protein HMGB2 is limited to your SZ, which consists of cells expressing mesenchymal stem cell markers. (cox-inhibitors.com)
Lipopolysaccharide (LPS) from Salmonella abortus equi , a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. (biomedcentral.com)

The cells are most commonly derived from the immune system with the goal of improving immune functionality and characteristics. (wikipedia.org)
In vivo, this electrical charge guided the particles towards the patient's dendritic immune cells that specify immune system targets. (wikipedia.org)
In an era of advanced nanomedicine, it should be possible to restrain or reprogram the immune system directly using genetic engineering (Chapters 19 and 23 ), or by using other means (e.g., temporary systemic white cell sequestration), to reduce or eliminate immunoresponsiveness during the period of nanomedical treatment. (nanomedicine.com)
Bottom line: Taken jointly, this created camelid-specific anti-CTLA-4 Nanobody 3hCTL55, chosen from a high-quality immune system collection by phage screen technique, could be effective for even more study about cancers medical diagnosis and cancer-therapy reasons. (radarcon2008.org)
While earlier work characterized PDAC as an immunologically privileged tumor poorly recognized by the immune system, recent studies revealed the important and nuanced roles of immune cells in the pathogenesis of PDAC. (stanford.edu)
DCs comprise a family of antigen presenting cells that act like 'conductors' of the immune response in their capacity to orchestrate signals derived from the different parts of the immune system [ 2 ]. (biomedcentral.com)

Recent isolation and detection techniques also remained controversial. (biomedcentral.com)
With the development and improvement of isolation and detection tools that enable the discovery of exRNAs, researchers in nephrology have detected exRNAs in various body fluids, particularly blood and urine. (biomedcentral.com)
In this review, we will overview of the characterization of exRNA, summarize the current cognition about the role of exRNAs in renal diseases and compare the isolation and detection techniques. (biomedcentral.com)

The Compact disc11b+ Kupffer cells portrayed intracellular TNF and surface area Fas-ligand (FasL). (enmd-2076.com)

Antigen nonspecific methods include the use of cytotoxic drugs that interfere with all cell division in the body [ 383 ]. (nanomedicine.com)

First described by Cooper and Travers in 1818, giant cell tumors (GCTs) of bone have been labeled the most challenging benign bone tumors. (medscape.com)
Rosario M, Kim HS, Yun JY, Han I. Surveillance for lung metastasis from giant cell tumor of bone. (medscape.com)
Giant-cell tumor of bone: analysis of two hundred and eight cases in Chinese patients. (medscape.com)
Pulmonary metastasis of benign giant cell tumor of bone. (medscape.com)
We studied 15 consecutive patients with high-risk or relapsed CD19 + B-ALL treated with CD19 CAR-T cell therapy on the CARPALL study ( NCT02443831 ) and in whom adequate CAR-T cells could be isolated for subsequent analyses from cryopreserved samples of blood or bone marrow. (nature.com)
These agents also damage all tissues (e.g., gut epithelium, bone marrow) where rapid cell division is occurring, creating other undesirable side effects, thus often may not be suitable for use in medical nanorobotics. (nanomedicine.com)
For example, mice genetically deficient for adaptive immunity displayed accelerated bone healing. (hindawi.com)
letters to the editor: bone marrow transplantation and leukemic transformation of transplanted cells]. (liverpool.ac.uk)

We used epigenetic profiling by time-of-flight (EpiTOF) to investigate changes in histone modifications within peripheral blood mononuclear cells from IBD patients.We discovered substantial heterogeneity in histone modifications across multiple immune cell types in IBD patients. (stanford.edu)

Administration of IL-2 after cell transfer enhanced therapeutic potential. (wikipedia.org)
While increased CD16 expression was transient in nature, this may not negatively impact the therapeutic potential of engineered NK cells due to the short persistence of adoptively transferred NK cells. (maxcyte.com)

Several molecular platforms have been used to investigate the molecular profile of the disease, from high throughput gene expression analyses down to single cell biological dissection. (oaepublish.com)
CD16 expression correlated with the concentration of CD16-158V mRNA used during electroporation and ranged from a 2-fold increase using 1 µg/10 6 NK cells up to a 3-fold increase using 8 µg/10 6 NK cells (data not shown). (maxcyte.com)
CD16 expression peaked 24 hours post electroporation and remained higher than non-electroporated NK cells for up to 72 hours post electroporation (Figure 1B). (maxcyte.com)
Non-electroporated, ex vivo expanded NK cells were used as a control to assess endogenous CD16 expression. (maxcyte.com)
BD ® AbSeq Antibody-Oligonucleotide Conjugates together with the BD Rhapsody TM mRNA Sequencing Kits enable simultaneous detection of proteins and mRNA expression in a single experiment and help deepen your understanding of protein biology and accelerate your single-cell research. (bdbiosciences.com)
However, as the present case will illustrate, selective pressures in vivo may cause differential expression of certain GBS surface components during colonization or dissemination. (cdc.gov)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
Astrocytes augmented HIF-1α expression in glioblastoma cells under hypoxia. (cancerindex.org)
The expression of HIF-1 downstream genes, cancer colony formation, and Matrigel invasion of glioblastoma cells were stimulated by conditioned medium from astrocytes pre-exposed to hypoxia. (cancerindex.org)
We have recently found abundant and selective expression of Adamdec1 in colonic mucosal PDGFRalpha+ cells. (stanford.edu)
2nd, since TowneBAC consists of a GFP expression cassette, fluorescent microscopy Inhibitors,Modulators,Libraries was utilized to detect GFP expression and to visualize contaminated cells. (mirnaarray.com)
2013) exhibited the elevated expression of cysteine protease (cathepsin B alone or with uPAR) in glioblastomas, which in turn was responsible of self-renewal of malignant glioblastoma stem cells. (globaltechbiz.com)
Inhibition of IRAK4-L abrogates leukaemic growth, particularly in AML cells with higher expression of the IRAK4-L isoform. (cancer-genetics.org)

We have previously shown that TACI stimulation in primary mouse B cells is inefficient using soluble trimeric BAFF or APRIL, but requires higher-order multimeric forms of the ligands that probably mimic the membrane-bound ligand [10]. (bso14.org)
Membrane-bound BAFF may thus be an important ligand for TACI, and conversely TACI may induce signalling in BAFF-expressing cells. (bso14.org)

B-lineage acute lymphoblastic leukemia (B-ALL) is the most common type of childhood cancer and mostly derives from immature B cells that carry the cell surface antigen CD19 (ref. 1 ). (nature.com)
The gene is preferentially expressed by immature dendritic cells and memory T cells. (cancerindex.org)

NK cells were isolated from healthy peripheral blood mononuclear cells (CD16-158F/F donors) and expanded ex vivo for 11-15 days with irradiated EBV-SMI-LCL cells. (maxcyte.com)

First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)

They had a higher proportion of less differentiated CD34 + hematopoietic progenitors, and a subset of CD56 bright NK cells and γδ T cells characterized by distinct histone modifications associated with the gene transcription. (stanford.edu)
ExRNAs may be transferred between cells and have functional significance in health and diseases by horizontal gene regulation. (biomedcentral.com)
Many RNA species have been found in eukaryotic cells by interpretations of gene transcription within genomes. (biomedcentral.com)

Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)

One particular element that is beginning to garner interest involves the systemic mobilization of endothelial progenitor cells (EPCs). (biomedcentral.com)
Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. (biomedcentral.com)
EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. (biomedcentral.com)
Blood-derived endothelial progenitor cells (EPCs) represent the "promoters" of vascular repair providing the rationale for autologous stem cell therapy [ 2 ]. (biomedcentral.com)

As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in 2016, lung cancer, breast cancer, sarcoma and melanoma. (wikipedia.org)
Using a sensitive tandem mass spectrometry technique, we detected the presence of MELOE-3 in total lysates of both melanoma cells and normal melanocytes. (oncotarget.com)

A modified immunohistochemical method detecting cytokine producing rather than cytokine binding cells was applied to determine cytokine synthesis in fixed cryopreserved sections. (bmj.com)
6-16 However, most previous studies based on immunohistochemical staining of intra-articular cytokine production in RA have been performed on acetone fixed tissue, a procedure which does not readily allow subsequent discrimination of cytokine producing cells and cytokine binding cells. (bmj.com)
phagocytic and ROS making capacity, and Compact disc11b+ Kupffer cells with cytokine-producing capability. (enmd-2076.com)

Anatomy9

Diseases4

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes4

Information Science4

Differentiation17

Monocytes6

Subsets6

Diseased tissues1

Proliferation7

Assays5

Umbilical8

Macrophages7

MSCs11

Receptor15

Antigens9

Cytometry14

Leukocyte1

Apical2

Monocyte-derived1

Adult4

Activating receptors1

Inflammatory11

Stromal cells1

Dendritic cell2

Play a pivotal role3

Role of dendritic cells2

Apoptosis1

Cytokines4

Clinical14

Mediate4

Human11

Assay7

Vitro10

Tumor cells2

Antibodies5

Lymphoid4

Phenotype5

Microenvironment2

Spleen2

Responses5

Adoptive1

Populations5

Myeloid cells1

Pathways1

Markers2

Immune system6

Isolation3

Intracellular1

Cytotoxic1

Bone8

Heterogeneity1

Therapeutic potential2

Expression13

Ligand2

Immature2

Donors1

Potentially1

Gene3

Inhibitory1

EPCs5

Melanoma2

Cytokine3