• After embryo aneuploidy, parental chromosomal translocations, maternal thrombophilic anomalies, immunological disorders, and obvious uterine ultrastructural abnormalities are excluded as etiologic contributors, implantation failure intrinsic to the endometrium is commonly suspected as an underlying cause of early pregnancy loss [EPL ( 1 )] and recurrent pregnancy loss [RPL ( 2 )], the latter defined as the loss of two or more consecutive pregnancies in the first trimester ( 2 - 5 ). (frontiersin.org)
  • Accordingly, pregnancy success rates currently achieved by natural or assisted conception for women at high-risk for EPL or RPL can only be increased by an improved understanding of the cellular and molecular mechanisms that control endometrial function during the periimplantation period. (frontiersin.org)
  • For the implantation groups, uteri were obtained on D4, D5 and D6 (day of implantation) of pregnancy. (jri.ir)
  • This review summarizes new knowledge on expression of genes and provides insights into approaches for study of conceptus-endometrial interactions in ruminants with emphasis on the peri-implantation stage of pregnancy. (bioscientifica.com)
  • The RIF group included 20 women who underwent ≥ 3 ETs, including a total of ≥ 4 good-quality embryos, without pregnancy, whereas the control group included 10 women who had given birth at least once in the past year. (biomedcentral.com)
  • However, in IVF cycles, many couples with infertility fail to achieve pregnancy repeatedly with no signs of embryo implantation or production of HCG. (biomedcentral.com)
  • Recurrent implantation failure (RIF) is defined as failure to achieve a clinical pregnancy after transfer of at least four good-quality embryos (score ≥ 7) in a minimum of three fresh or frozen cycles [ 4 ]. (biomedcentral.com)
  • Exposure to stress strongly influences hypothalamic-pituitary-adrenal axis and elevated glucocorticoid levels suppress various ovarian functions including implantation thereby pregnancy. (jrhm.org)
  • Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals. (animal-reproduction.org)
  • 2021: Christina Alexandra KALENBERG (Division of Veterinary Anatomy, University of Bern, Länggassstrasse 120, 3001 Bern, Switzerland) for the paper entitled "The embryonic development of the bovine stomach revisited" in co-authorship with M.H. Stoffel and published in the Journal Anatomia Histologia Embryologia 2020, 49:270-280. (wava-amav.org)
  • 2018: Miriam Beyes (Institute of Veterinary Medicine, Georg-August-Universität, Göttingen, Germany) for the paper entitled: "Description of post-implantation embryonic stages in European roe deer (Capreolus capreolus) after embryonic diapause" in co-authorship with N. Nause, M. Bleyer, F.-J. Kaup and S. Neumann and published in the Journal Anatomia Histologia Embryologia 2017, 46:582-591. (wava-amav.org)
  • showed, for the first time, that RHAMM is differentially expressed during all stages of preimplantation human embryos and human embryonic stem cells (hESC), and indicated that RHAMM knockdown results in down-regulation of several pluripotency markers in hESCs, induction of early extraembryonic lineage, loss of cell viability, and changes in hESC cycle (2). (jri.ir)
  • Choline acts during preimplantation development of the bovine embryo to program postnatal growth and alter muscle DNA methylation. (animal-reproduction.org)
  • This paper was applauded for using state-of-the-art imaging and computational morphometric analysis of the pre- and postnatal developmental changes in the shape and composition of the murine femur, providing new insights in the ossification and growth patterns of developing bone. (wava-amav.org)
  • Ozbilgin K, Boz B, Tuğyan K, Inan S, Vatansever S. RHAMM Expression in the Rat Endometrium during the Estrous Cycle and following Implantation. (jri.ir)
  • Changes in pinopode development and abnormal expression of miR-30d-5p, SOCS1 and LIF pathway in the endometrium could be the reasons for implantation failure. (biomedcentral.com)
  • During WOI, the endometrium undergoes well-defined morphological and molecular changes that allow embryos to adhere and invade, including the developing pattern of microvilli of the epithelial cells and pinopode growth, and alterations in various signalling molecules and inflammatory cytokines [ 6 ]. (biomedcentral.com)
  • In some species (e.g., bovine), evidence indicates that decreases in IGFBP-4 and -5 levels in estrogen-dominant preovulatory follicles are likely due, in part, to increased protease activity, whereas lower levels of IGFBP-2 are not due to increased proteolysis. (bioone.org)
  • Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)
  • The mammalian ovary has all the molecular machinery required for the biosynthesis of melatonin and interestingly concentration of melatonin in the follicular fluid of pre-ovulatory follicles is much higher than circulatory melatonin even in humans. (jrhm.org)
  • Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
  • In this study, we focus on the expression of RHAMM in the rat uterus during estrous cycle and implantation period. (jri.ir)
  • The uterus undergoes extensive remodeling during estrous cycle and embryo implantation (10). (jri.ir)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • Poor endometrial receptivity is a major factor that leads to recurrent implantation failure. (biomedcentral.com)
  • Various studies have indicated that microRNAs (miRNAs) are involved in multiple processes of embryo implantation, but the role of miRNAs in endometrial receptivity in patients with recurrent implantation failure (RIF) remains elusive. (biomedcentral.com)
  • Endometrial tissue samples were collected between January 2018 to June 2019 from two groups of women who underwent in vitro fertilisation and embryo transfer (IVF-ET) or frozen ET. (biomedcentral.com)
  • An endometrial biopsy was performed during the implantation window (LH + 7). (biomedcentral.com)
  • One possible cause of the unsuccessful implantation rate is reduced endometrial receptivity, although the transferred embryos are of high quality [ 3 ]. (biomedcentral.com)
  • Therefore, the assessment of endometrial receptivity is of utmost importance in the IVF procedures to increase the rate of implantation in patients with RIF. (biomedcentral.com)
  • Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). (animal-reproduction.org)
  • The paper is a very comprehensive and well-illustrated micro-anatomical and ultrastructural developmental study on the altering layout of the bovine forestomachs in the 26-80 days old embryo. (wava-amav.org)
  • Methods: Twenty male patients, ages ranged from 55 to 70 years, stage ΙΙ & ΙΙΙ according to modified Hoehn and Yahr (1997) classification of disabilities and ten male healthy elderly subjects, ages ranged from 55 to 70 years, participated in this study. (civilenvironjournal.com)
  • Soon after embryo attachment and the early stages of implantation, further invasion into the maternal compartment requires a functional decidua ( 6 ). (frontiersin.org)
  • WIDER IMPLICATIONS OF THE FINDINGS: The results are reassuring and indicate that the embryo biopsy itself has no adverse effect on the perinatal, early childhood, or maternal outcomes. (bvsalud.org)
  • Methylome dynamics of bovine gametes and in vivo early embryos. (animal-reproduction.org)
  • STUDY QUESTION: Is preimplantation genetic testing (PGT) associated with adverse perinatal outcome and early childhood health? (bvsalud.org)
  • These results suggest that embryo DMRs were reprogrammed during the final stages of de novo methylation during embryogenesis or later in development. (animal-reproduction.org)
  • Canovas S, Ross PJ, Kelsey G, Coy P. DNA Methylation in Embryo Development: Epigenetic Impact of ART (Assisted Reproductive Technologies). (animal-reproduction.org)
  • Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf J, Reik W. Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. (animal-reproduction.org)
  • Despite advances in the understanding of implantation failure, the pathogenesis of RIF is complex and requires a multidimensional explanation. (biomedcentral.com)
  • It is known that RHAMM plays an important role in several cellular events, but the role of RHAMM during estrous cycle and embryo implantation has not been investigated much. (jri.ir)
  • The expression level of miR-30d-5p decreased in the endometria during the implantation window of patients with RIF, whereas the mRNA and protein levels of SOCS1 were significantly higher in the RIF group than in the control group. (biomedcentral.com)
  • In addition, a significant decrease in LIF and p-STAT3 expression was observed during the implantation window in patients with RIF. (biomedcentral.com)
  • Investigation the influences of vitamin (vit) E over a period of 5 days on the pharmacokinetics of florfenicol after single and multiple oral administrations in broiler chickens. (civilenvironjournal.com)
  • Total of 12 broiler chickens had been taken single and multiple oral administrations of florfenicol (30 mg/kg) alone or pre-treated with vit E (2 mg/kg). (civilenvironjournal.com)
  • The mean serum concentrations of florfenicol alone were markedly lower when compared with florfenicol pre-treated with vit E after single and multiple dosing. (civilenvironjournal.com)
  • While, after multiple dosing, (Cmax) were 7.4±0.3, 8.04±0.3 ug/ml, (t0.5ab) 0.82±0.04, 0.81±0.04 h and (t0.5 el) 3.77±0.2, 4.52±0.7 h after multiple dosing of florfenicol alone and florfenicol pre-treated with vit E respectively. (civilenvironjournal.com)
  • In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. (animal-reproduction.org)
  • In the present study, we investigated the presence of pinopodes and the roles of miR-30d-5p, suppressor of cytokine signalling 1 (SOCS1) and the leukaemia inhibitory factor (LIF) pathway in women with a history of RIF during the implantation window. (biomedcentral.com)
  • We know that every client is unique, from a different background, at a different life stage, with different needs. (repromed.co.nz)
  • PGT, an invasive technique, requires genetic analysis of one or up to ten cells from the embryo and is combined with IVF or ICSI. (bvsalud.org)
  • Singletons born after PGT were compared with all singletons born after IVF/ICSI (n = 61 060) born during the same period of time and with a matched sample of singletons (n = 42 034) born after spontaneous conception selected from the Medical Birth Register. (bvsalud.org)
  • While methods for in vitro culture of pre- and peri-implantation mouse embryos are routinely used, approaches for efficient and stable culture of post-implantation embryos from egg cylinder stages until advanced organogenesis remain to be established. (researchsquare.com)
  • These systems support stable growth of normal mouse embryos ex utero from pre-gastrulation to advanced organogenesis. (researchsquare.com)
  • While mouse embryos are consistently cultured through pre- and peri-implantation development 3,4 , establishing culture conditions sustaining proper long-term development of post-implanted mouse embryos outside the uterine environment remains challenging. (researchsquare.com)
  • Here, we describe the step by step protocol of our recently described embryo culture system that allows sustained mouse embryo development from pre-gastrulation to advanced organogenesis stages, enabling the application and monitoring of external and internal manipulations in mouse embryos over up to six days of post-implantation development. (researchsquare.com)
  • Here we report an efficient method for dsRNA-mediated gene silencing in late cleavage-stage mouse embryos that permits examination of phenotypes at post-implantation stages. (biomedcentral.com)
  • The intrauterine confinement of the developing mammalian embryo has been the main limiting factor for studying post implantation embryogenesis 1,2 . (researchsquare.com)
  • The ability to remove a mammalian embryo from the uterine environment and grow it normally in controlled conditions represents a valuable tool to characterize the effect of different perturbations on development during the period from gastrulation to organogenesis, that can be combined with genetic modification, chemical screens, tissue manipulation and microscopy methods. (researchsquare.com)
  • Embryonic patterning in mammalian peri-implantation development is a complex process requiring many signaling events to establish the future body plan. (biomedcentral.com)
  • These include severe delays in post-implantation development and defects in the anterior midline and neural folds at headfold stages. (biomedcentral.com)
  • Bovine testis tissue xenografts contain elongating spermatids 6 mo after grafting. (bioone.org)
  • The objective of this research was to xenograft testis tissue from multiple ages of bull calves for 24 or 36 wk in order to gain a better understanding of early bovine testis development. (bioone.org)
  • Testis tissue grafts from 1- and 8-wk-old calves had elongating spermatids in greater than 5.5% of seminiferous tubule cross sections at the time of graft removal regardless of grafting period. (bioone.org)
  • These data demonstrate that both donor age and grafting period may be important factors regulating the maturation of bovine testis xenografts, indicating that intrinsic differences exist within testis tissue at these donor ages. (bioone.org)
  • These data provide the framework for further study of bovine spermatogenesis using ectopic testis xenografting. (bioone.org)
  • Bovine ectopic testis tissue grafting is a technique that can be used to study bovine spermatogenesis and for the production of germ cells for a variety of applications. (bioone.org)
  • 2021: Christina Alexandra KALENBERG (Division of Veterinary Anatomy, University of Bern, Länggassstrasse 120, 3001 Bern, Switzerland) for the paper entitled "The embryonic development of the bovine stomach revisited" in co-authorship with M.H. Stoffel and published in the Journal Anatomia Histologia Embryologia 2020, 49:270-280. (wava-amav.org)
  • 2018: Miriam Beyes (Institute of Veterinary Medicine, Georg-August-Universität, Göttingen, Germany) for the paper entitled: "Description of post-implantation embryonic stages in European roe deer (Capreolus capreolus) after embryonic diapause" in co-authorship with N. Nause, M. Bleyer, F.-J. Kaup and S. Neumann and published in the Journal Anatomia Histologia Embryologia 2017, 46:582-591. (wava-amav.org)
  • We detail the enhanced roller culture system protocol that supports growth of E7.5 late-gastrulating embryos (neural plate and headfold-stage) until the hind limb formation stage (~E11), as well as the extended culture from E5.5/6.5 combining static and roller culture platforms. (researchsquare.com)
  • The mean serum concentrations of florfenicol alone were markedly lower when compared with florfenicol pre-treated with vit E after single and multiple dosing. (civilenvironjournal.com)
  • The peak serum concentrations (Cmax) were 5.9±0.46, 7.48±0.3 ug/ml, absorption half-life (t0.5ab) of 0.51±0.06, 0.71±0.1 h and elimination half-life (t0.5el) of 2.72±0.34, 3.34±0.5 after single florfenicol alone and florfenicol pre-treated with vit E respectively. (civilenvironjournal.com)
  • The high-throughput or 'omic' approaches, including RNA sequencing, microarray and proteome, has been applied to profile the expression patterns of genes or proteins for the endometrium during peri-implantation period. (biomedcentral.com)
  • The proposed approach constitutes a powerful tool to screen the functions of genes that govern the development of the mouse embryo. (biomedcentral.com)
  • This approach is a valuable way of targeting knock-down to the embryo proper, but does not allow targeting of genes expressed in extraembryonic tissues such as the extraembryonic ectoderm (ExE) and the visceral endoderm (VE) that do not arise from the ES cell progeny. (biomedcentral.com)
  • We wished to enable studies of the effects of knocking-down single or multiple genes simultaneously upon patterning of the early post-implantation mouse embryo. (biomedcentral.com)
  • The paper is a very comprehensive and well-illustrated micro-anatomical and ultrastructural developmental study on the altering layout of the bovine forestomachs in the 26-80 days old embryo. (wava-amav.org)
  • Investigation the influences of vitamin (vit) E over a period of 5 days on the pharmacokinetics of florfenicol after single and multiple oral administrations in broiler chickens. (civilenvironjournal.com)
  • In these protocols, late gastrulating embryos (E7.5) are grown in 3D rotating bottles settings, while extended culture from pre-gastrulation stages (E5.5 or E6.5) requires a combination of static and rotating bottle culture protocols. (researchsquare.com)
  • To this end we have developed a method for gene silencing that allows dsRNA to be introduced into whole embryos at late pre-implantation stages such that they can develop in utero and be examined post-implantation (Additional File 1 ). (biomedcentral.com)
  • Several members of the team are involved in platforms or shared facilities (MIMA2 platform - https://www6.jouy.inrae.fr/mima2_eng/MIMA2_platform - , BREED unit ruminant embryo production facility). (inrae.fr)
  • The results could help us to better understand the implantation in the ewes. (biomedcentral.com)
  • Implantation, the sign and initial phase of pregnancy, is a process lading to attachment of developing conceptus to the maternal endometrium, and resulting in the establishment of placental structure. (biomedcentral.com)
  • These two areas play different roles in implantation process, and both are essential for the establishment of pregnancy. (biomedcentral.com)
  • Although implantation is of prime importance for establishment of pregnancy, the underlying mechanisms responsible for this complex physiological process, are still unclear. (biomedcentral.com)
  • This paper was applauded for using state-of-the-art imaging and computational morphometric analysis of the pre- and postnatal developmental changes in the shape and composition of the murine femur, providing new insights in the ossification and growth patterns of developing bone. (wava-amav.org)
  • However, its application at later stages could be considered more demanding as from the 8-cell stage onwards, some cells become internalized and therefore less accessible. (biomedcentral.com)
  • Ovine has been extensively used as model to research maternal-fetal dialogue during implantation [ 3 ]. (biomedcentral.com)
  • Consequently, the sequence of developmental events occurring from pre-gastrulation to organogenesis, remains to be fully understood and hard to manipulate. (researchsquare.com)