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  • region
  • We determined that a 10-nucleotide oligomer of peptide nucleic acid (PNA) targeting the start codon region of an essential gene acpP is able to cause complete growth inhibition of E. amylovora . (frontiersin.org)
  • found
  • We found that conjugation of cell penetrating peptide (CPP) to PNA is essential for the antimicrobial effect, with CPP1 [(KFF)3K] being the most effective against E. amylovora . (frontiersin.org)
  • Amino
  • The latter represent an assortment of fairly unrelated sequences essentially characterised by a high content of basic amino acids and a length of 10-30 residues. (mdpi.com)
  • Neurons can also communicate with one another by releasing peptides (strings of amino acids bonded together) into the synapse. (mayo.edu)
  • 11. The water-soluble polymer linked to a solid support of claim 1 , wherein the water-soluble polymer consists essentially of monomer units able to undergo synthesis on a solid support, the monomer units being selected from the group consisting of amino acids, peptide nucleic acids, nucleotide monophosphate monomer units, and sugar monomer units. (google.com)
  • 12. The water-soluble polymer linked to a solid support of claim 1 , wherein the first cleavage segment comprises a sequence of at least two linked monomer units, each monomer unit selected from the group consisting of an amino acid monomer, a sugar monomer and a nucleotide, and wherein the water-soluble polymer is selectively cleavable from said support in the presence of an enzyme. (google.com)
  • Almost all discussions of prebiotic chemistry assume that amino acids and nucleotides condensed non-enzymatically to form oligomeric products. (scripps.edu)
  • Our lab showed that carbonyl sulfide (COS), a simple gas that is present in the emissions from present-day volcanoes, is a condensing agent that brings about the formation of peptides from amino acids under mild conditions in aqueous solution. (scripps.edu)
  • analogs
  • Several classes of nucleic acid analogs have been reported, but no synthetic informational polymer had previously proven responsive to selection pressures under enzyme free conditions. (scripps.edu)
  • CPPs
  • Among these synthetic vectors are cationic polymers, branched dendrimers, cationic liposomes and cellpenetrating peptides (CPPs). (mdpi.com)
  • CPPs are capable of mediating the cellular uptake of hydrophilic macromolecules like peptides and nucleic acids (e.g. siRNAs, aptamers and antisenseoligonucleotides), which are internalised by cells at a very low rate when applied alone. (mdpi.com)
  • mRNA
  • In this study, we have examined the potential of peptide nucleic acid (PNA) 9-aminoacridine conjugates to modulate the pre-mRNA splicing of the mdm2 human cancer gene in JAR cells. (biomedcentral.com)
  • MRNA ), a leading nucleic acid-based drug discovery and development company, today announced that the U.S. Patent and Trademark Office (USPTO) has issued a Notice of Allowance for patent application U.S. 11/955,207 with claims that cover a library of over 1x10(15) novel peptides. (marketwired.com)
  • mimic
  • Although most prior research on PNA has focused on its nucleic acid-like character, recent developments of the peptide-like aspects of PNA promise to stimulate additionally the work on this fascinating DNA mimic. (schoolbag.info)
  • oligomer
  • We determined that a 10-nucleotide oligomer of peptide nucleic acid (PNA) targeting the start codon region of an essential gene acpP is able to cause complete growth inhibition of E. amylovora . (frontiersin.org)
  • We designed and characterized an oligomer family that efficiently self-assembles via reversible covalent anchoring of nucleobase recognition units onto simple oligo-dipeptide backbones (thioester peptide nucleic acids, tPNA) and undergoes dynamic sequence modification in response to changing templates in solution. (scripps.edu)
  • ligation
  • After ligation to the modular peptide-based BioShuttle carrier, which was utilized to facilitate the delivery of the functional modules into the cells' cytoplasm, the modules were scrutinized. (medsci.org)
  • penetratin
  • In contrast, PNA TAR -penetratin conjugate is moderately immunogenic mainly due to its penetratin peptide component. (pubmedcentralcanada.ca)
  • The conjugates with penetratin, transportan-21 and tat-peptides were most effective as an anti-HIV virucidal agents with IC 50 values in the range of 28-37 nM while IC 50 for inhibition of HIV-1 replication was lowest with PNA TAR -transportan-27 (0.4 μM) followed by PNA TAR -tat (0.72 μM) and PNA TAR -penetratin (0.8 μM). (pubmedcentralcanada.ca)
  • chemistry
  • They are assembled using techniques adapted from peptide chemistry. (currentprotocols.com)
  • In this context our modified solid phase peptide chemistry using temperature shifts during synthesis is considered as an appropriate technology. (medsci.org)
  • Nowadays the solid phase synthesis (SPPS) chemistry emerges as a key technology and is considered as a promising methodology to design peptides for the investigation of molecular pharmacological processes at the transcriptional level. (medsci.org)
  • Guidelines for synthesis of PNA using an automated DNA synthesiser are provided here, however these could be easily applied to other automated instruments capable of synthetic chemistry, in fact many users have successfully transferred this synthesis to microwave assisted peptide synthesisers. (linktech.co.uk)
  • In general, an appropriate solid support must be cleavable in acid, leaving a C-terminal amide, and be compatible with Fmoc chemistry. (linktech.co.uk)
  • aqueous
  • 2. A method according to claim 1 wherein the nucleic acid solution is aqueous. (google.com)
  • We designed, synthesized, and characterized alpha-helical peptides that act autocatalytically in templating their own synthesis by accelerating the thioester-promoted amide-bond condensation of shorter fragments in neutral aqueous solution. (scripps.edu)
  • constructs
  • Feasible patient-specific theranostic platforms in the personalized medicine are particularly dependent on efficient and clinically applicable peptide constructs. (medsci.org)
  • Peptides capable of directed delivery are expected to further improve the delivery efficiency of the Company's UNA and CRN substituted nucleic acid constructs, which have demonstrated in vivo activity and efficacy against targets in both normal/healthy and disease models, respectively. (marketwired.com)
  • vivo
  • One goal of this investigation was to develop a polymer conjugated with multiple copies of peptide nucleic acid (PNA) and with pharmacokinetic properties suitable for applications in vivo. (umassmed.edu)
  • novel
  • A primary advantage of this patented peptide library is the ability to rapidly screen and identify novel peptides that exhibit cell specific targeting characteristics for directed delivery of nucleic acid therapeutics," said Barry Polisky, Ph.D., Chief Scientific Officer of Marina Biotech. (marketwired.com)
  • delivery platforms
  • This technology is directly applicable to the Company's DiLA 2 and SMARTICLES delivery platforms as peptides are readily conjugated to the components of these delivery platforms. (marketwired.com)
  • recognition
  • But only now emerging is the recognition that peptides can Self-assemble into a wide variety of non-protein-like structures, including fibrils, fibres, tubules, sheets and monolayers. (springer.com)
  • efficiently
  • Earlier, we have shown that an anti-TAR polyamide nucleotide analog (PNA TAR ) conjugated to a membrane transducing (MTD) peptide, transportan, is efficiently taken up by the cells and displays potent antiviral and virucidal activity [B. Chaubey, S. Tripathi, S. Ganguly, D. Harris, R. A. Casale and V. N. Pandey (2005) Virology , 331, 418- (pubmedcentralcanada.ca)
  • self-assembly
  • We have sought to converge the two distinct fields of DNA and peptide self-assembly by using an artificially synthesised polymer that has attributes of both chemical families. (esrf.eu)
  • reminiscent
  • Our studies have ranged from defining simple condensation reagents that could have acted on the early Earth, to the development of replicating peptides that participate in chemical systems displaying behavior reminiscent of biology, such as symbiosis or parasitism. (scripps.edu)
  • virucidal
  • These results indicate that anti-HIV-1 PNA conjugated with MTD peptides are not only inhibitory to HIV-1 replication in vitro but are also potent virucidal agents which render HIV-1 virions non-infectious upon brief exposure. (pubmedcentralcanada.ca)
  • Functional
  • Our approach made use of the sequence-dependant auto- and cross-catalytic functional characteristics of template-directed peptide fragment condensation reactions. (scripps.edu)