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  • Biophysical
  • The interplay of many factors, including the alteration of the biophysical properties of the macromolecules upon association and their consequent lateral reorganization at the membrane surface, presents exciting challenges to biophysicists and biochemists. (ccl.net)
  • Particularly, the role of lipid composition and self-organization in regulating the membrane biophysical properties has been investigated. (unina.it)
  • We compared different biophysical techniques to gain insight into the properties of the electrical surface potentials of lipid monolayers and aggregates composed of LPSs and various phospholipids and their interaction with peptides and proteins. (biomedcentral.com)
  • fusion protein
  • In the system we use here ubiquitin formation leads to proteolytic cleavage liberating a transcription factor (PLV) from the C-ubiquitin (C) fusion protein which can then activate reporter genes. (pubfacts.com)
  • As predicted, the N-domain of the fusion protein alone was shown to act as a mitochondrial targeting signal, whereas the C-domain alone functions as an export signal. (paperity.org)
  • C, Schematic of the pExg2Met-Dest vector used to express the bait fusion protein, including the N-terminal fusion with the GPI signal sequence (see also Supplemental Fig. S1 ). (plantphysiol.org)
  • First, membrane anchoring of the bait fusion protein is vital, as a soluble bait could transit to the nucleus and activate reporter gene expression even in the absence of prey binding. (plantphysiol.org)
  • interaction
  • The past years have seen a massive accumulation of binary protein-protein interaction data. (pubfacts.com)
  • It is based on the formation of a quasi-native ubiquitin structure upon interaction of two proteins to which the N- and C-terminal halves of ubiquitin have been fused. (pubfacts.com)
  • It was expected that peptides with sequences contained in these loops would mimic the homophilic loop-loop interaction and activate the docking gate. (physiology.org)
  • Microstructural characterization of phospholipid membranes and study of their interaction with peptides and proteins. (unina.it)
  • In the latter part of the research, the interaction of these lipid systems with peptides deriving from different viral glycoproteins as well as from the A peptide was analyzed. (unina.it)
  • However, the influence of peptides/proteins on the ζ-potential can be used to characterize the underlying interaction mechanisms. (biomedcentral.com)
  • Localization
  • Our present study demonstrates the localization of the 3 variant proteins in the PM of cancer cells, suggesting a possible role for these membrane-localized MDM2 variant in the action of PNC-27. (aacrjournals.org)
  • amino acid residues
  • Here we investigated the structure and function of exceptionally long signal peptides encompassing at least 40 amino acid residues. (paperity.org)
  • This is the case when all membrane-anchoring parts of the proteins ( amphiphilic alpha helices , exposed nonpolar residues, or lipidated amino acid residues ) are missing in the experimental structures. (wikipedia.org)
  • biological
  • Membrane proteins define biological functions of membranes in cells. (pubfacts.com)
  • Through the controlled release of membrane-anchored dormant transcription factors or other effectors, these proteases play a pivotal role in biological and pathological processes ( 2 - 4 ). (pnas.org)
  • Biological membranes act as selective barriers that separate the interior of cells from their outside environment. (stanford.edu)
  • Membrane proteins play crucial roles in a wide range of biological and physiological processes and are targeted by a large number of pharmacologically active compounds including ~ 50% of the drugs in use today. (stanford.edu)
  • enzymatic hydrolysis
  • Heat pre-treatment of SPI at 95 degrees celsius (C) for 5 min prior to enzymatic hydrolysis was investigated for its effect on peptide distribution and antioxidant capacity. (uwaterloo.ca)
  • processes
  • Connexin mimetic peptides are widely used to assess the contribution of nonjunctional connexin channels in several processes, including ATP release. (physiology.org)
  • These peptides are derived from various connexin sequences and have been shown to attenuate processes downstream of the putative channel activity. (physiology.org)
  • Rich structure of cell membranes raises broad number of questions regarding the mechanisms driving and regulating processes taking place on membranes. (cuni.cz)
  • We use this technique to study metabolic and membrane transport processes in cells, mostly the human erythrocyte. (edu.au)
  • outer membrane
  • In the case of Gram-negative bacteria this is the outer membrane (OM), the lipid composition of which is extremely asymmetric: Whereas the inner leaflet is composed of a phospholipid mixture, the outer leaflet is made up solely from lipopolysaccharides (LPSs). (biomedcentral.com)
  • The cell envelope of Gram-negative bacteria is composed of two membranes, the inner and the outer membrane. (biomedcentral.com)
  • Gene expression analysis on the effects of TP359 on outer membrane biogenesis genes underscored marked down-regulation, particularly of oprF , which encodes a major structural and outer membrane porin (OprF) in both strains studied, indicating that the peptide may cause deregulation of outer membrane genes and reduced structural stability which could lead to cell death. (biomedcentral.com)
  • mimic
  • gap junction mimetic peptides were originally designed to mimic the docking gate of gap junction channels ( 11 , 13 ). (physiology.org)
  • aggregation
  • resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. (nature.com)
  • mechanisms
  • Plastids are considered promising bioreactors for the production of recombinant proteins, but the knowledge of the mechanisms regulating foreign protein folding, targeting, and accumulation in these organelles is still incomplete. (unipd.it)
  • crucial
  • This study, which appears in the March 30 issue of Science, looks at how the binding of these designed peptides affects the crucial first steps in blood clotting. (sciencecodex.com)