• Fic domains are evolutionarily conserved domains in prokaryotes and eukaryotes that belong to the Fido domain superfamily. (wikipedia.org)
  • These dsRNA-mediated processes are evolutionarily conserved and are thought to be involved in transposon silencing in various species, although evidence of a natural role in the majority of human cells is still scant. (nih.gov)
  • These proteins contain a characteristic zinc finger motif (Cys-x2-Cys-x(16,17)-x2-Cys) which displays some similarity to the C4-type GATA zinc finger. (embl.de)
  • Both guanine nucleotide-exchange proteins and GTPase-activating proteins (GAPs) for ARFs have been cloned recently. (embl.de)
  • The major mammalian effector proteins for cNMP signaling encompass PKA, PKG, the exchange protein directly activated by cAMP (EPAC), hyperpolarization-activated cyclic nucleotide-gated cation channels (HCN) as well as cyclic nucleotide-gated ion channels (CNG). (uni-kassel.de)
  • In the endogenous presentation pathway (MHC class I), abundantly expressed intracellular proteins are processed by the immunoproteasome and proteasome to yield short peptide fragments that are transported into the endoplasmic reticulum and assembled together with the MHC-I heavy chain and β2-microglobulin light chain (β 2 m) by the peptide-loading complex ( 2 ). (frontiersin.org)
  • The highly homologous ERM (ezrin/radixin/moesin) proteins, molecular cross-linkers which connect the cell membrane with the underlying cytoskeleton, have molecular weights of 81, 80 and 78 kDa respectively. (embl-heidelberg.de)
  • HN - 2018 FX - ATPases Associated with Diverse Cellular Activities MH - AAA Proteins UI - D000074582 MN - D8.811.277.40.13 MN - D12.776.157.25 MS - A large, highly conserved and functionally diverse superfamily of NTPases and nucleotide-binding proteins that are characterized by a conserved 200 to 250 amino acid nucleotide-binding and catalytic domain, the AAA+ module. (nih.gov)
  • Such interactions depend on signal exchange between both participants and can be mediated by effector proteins that alter the host cell structure and function, allowing the establishment of the relationship. (biomedcentral.com)
  • Actins are highly conserved proteins that are involved in cell motility, structure and integrity. (thermofisher.com)
  • The CSP has received the most attention and has been, and still is, the subject of numerous trials based on a variety of peptides, recombinant proteins, modified virus vectors, plasmids and a large diversity of adjuvants and immunization regimens. (medscape.com)
  • MHC proteins bind peptide antigens within the cell, and display them at the cell surface for interaction with T cell receptors. (umassmed.edu)
  • MHC proteins are highly polymorphic, with allelic differences associated with differences in peptide binding preferences and in individual susceptibility to allergy, infection, and autoimmune disease. (umassmed.edu)
  • We study MHC proteins and their interactions with peptides through a combination of biophysical and cellular analysis. (umassmed.edu)
  • A conserved oligomerization domain in the disordered linker of coronavirus nucleocapsid proteins. (nih.gov)
  • Crk proteins activate the Rap1 guanine nucleotide exchange factor C3G by segregated adaptor-dependent and -independent mechanisms. (nih.gov)
  • Small RNAs and proteins of the Argonaut family (all of which contain the PAZ and PIWI conserved domains) assemble in the cytoplasm into an RNA-induced silencing complex (RISC). (nih.gov)
  • Another example of AMPylators that play a role in bacterial homeostasis is the class I Fic AMPylators (FicT), which modifies the GyrB subunit of DNA gyrase, the conserved tyrosine residue for ATP binding of ParE subunit at Topoisomerase IV. (wikipedia.org)
  • Many eukaryotic protein kinases are activated by phosphorylation on a specific conserved residue in the regulatory activation loop, a post-translational modification thought to stabilize the active DFG-In state of the catalytic domain. (elifesciences.org)
  • These results define the biophysical role of a highly conserved core residue located outside of a protein-binding interface, demonstrating that Tyr35 has little impact on the trypsin-bound BPTI structure and acts primarily to define the structure of the free protein so as to maximize binding affinity. (shengsci.com)
  • The cysteinyl residue of isolated alkali light chain 2 was biotinylated and the light chain was exchanged with that of heavy meromyosin in 4.7 M-NH4Cl. (shengsci.com)
  • 19 The oligomerization domain of NPM also contains a highly conserved motif that appears critical for mediating ADP-ribosylation factor (ARF) binding in vivo . (haematologica.org)
  • Consequently, our laboratory has focused on this superfamily of metabolically stable plant-derived peptides, particularly CRPs with molecular weights of 2 to 6 kDa. (biomedcentral.com)
  • All these effectors share a highly conserved cyclic nucleotide binding (CNB) domain. (uni-kassel.de)
  • Many of the known cytoplasmic effectors have a specific translocation motif that would allow them to get inside the cell, such as the RXLR (arginine, any amino acid, leucine, arginine) motif of Avr3a from Phytophthora spp. (biomedcentral.com)
  • Hydrodynamic, enzymatic, and spectroscopic studies have identified a peptide-dependent conformational change, in which the region ß 58-69 of the MHC protein folds over the bound peptide to trap it in the site, in the rate determining step for the overall peptide binding reaction. (umassmed.edu)
  • Binding of EF2·GTP to the ribosome was thought to eIF5B mutations in Switch I, an element conserved in induce a conformational change and promote ribosomal all GTP binding domains, impair GTP hydrolysis and translocation following peptide bond formation. (nih.gov)
  • The GAP function of the amino-terminal extension of ARD1 required two arginines, an intact zinc finger motif, and a group of residues which resembles a sequence present in Rho/Rac GAPs. (embl.de)
  • Comparative analysis of viral and proviral segment sequences revealed a sequence motif involved in the excision of proviral genome segments which is highly conserved in two other bracoviruses. (biomedcentral.com)
  • Other ADAR genes in vertebrates and ADAR genes in other organisms were identified either by PCR using primers derived from the deaminase motifs or by sequence homology. (biomedcentral.com)
  • Upon enhancing the sequence disorders are ceramide of one suppressor of turnover populations in the physiological much life of Strong cholesterol kinases and complete another peptide( Kim et al. (evakoch.com)
  • The crystal structure of an MHC-peptide complex revealed that peptides of completely different sequence can be accommodated in the site with almost no alteration of the MHC molecule, with binding specificity determined by subtle details of interactions in the side-chain binding pockets. (umassmed.edu)
  • While DLP family members are typically not highly conserved on the sequence level, with the exception of the GTPase domain (GD), the resolved structures reveal a conserved modular arrangement of all DLPs: The globular GD at the protein's N-terminus is typically followed by an α-helical bundle signaling element (BSE) or neck domain that connects the GD to an α-helical stalk or trunk domain. (nature.com)
  • The GD, the only structural element of DLPs that is conserved at the sequence level, is characterized by a low µM nucleotide binding-affinity plus a relatively high basal GTPase activity, at least when compared to other GTPases. (nature.com)
  • In continuing studies, we are investigating the mechanism of peptide-exchange catalysis by DM, a major unsolved problem with implications to other systems in which structural homologues function as chaperones or conformational catalysis. (umassmed.edu)
  • Grb2 is an adaptor protein connecting the epidermal growth factor receptor and the downstream Son of sevenless 1 (SOS1), a Ras-specific guanine nucleotide exchange factor (RasGEF), which exchanges GDP by GTP. (nih.gov)
  • Analysis of the X-ray structures of the two peptides bound to HLA-B*15:01 reveals drastically different conformations with measurable changes in the stability of the protein complexes, while the self-epitope is excluded from binding due to steric hindrance in the MHC groove. (frontiersin.org)
  • Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • To understand the molecular basis of the phenomenon we focussed on the putative caveolin-1 binding domain (CBD) located in the lumenal, juxtamembranal portion of the M2 matrix protein which has been identified in the motif-based search. (biomedcentral.com)
  • 2014 ). The evolutionary conserved ubiquitin-proteasome system (UPS) and autophagy-lysosome pathway are the two major protein clearance mechanisms in eukaryotes. (springer.com)
  • The binding of TrkA receptor by NGF causes the activation of the Ras/Mitogen activated protein kinase (MAPK) pathway, which leads to increased proliferation and cellular growth through extracellular signal-regulated kinase (ERK) signalling. (eonco.info)
  • The purified NS5 protein, but not the mutant NS5 protein with an Ala substitution at the first Asp of the RdRp-conserved GDD motif, exhibited template- and primer-dependent RNA synthesis activity using a poly(A) RNA template. (biomedcentral.com)
  • Diversity of Short Linear Interaction Motifs in SARS-CoV-2 Nucleocapsid Protein. (nih.gov)
  • Our phosphorylated ill download лелята is that the central resection platelets of the severe 1970s, However to any distinct influx, reside to a untreated clearance of the p38MAPK mouse failure reported to its retinal, Thus conserved genome. (evakoch.com)
  • Evolutionary conserved low-molecular-weight transmitters (glutamate, aspartate, glycine, GABA, and ATP) acted as coordinators of distinct locomotory and feeding patterns. (frontiersin.org)
  • This study examined whether the highly conserved cytoplasmic tail of podocalyxin also contributes to the unique organization of podocytes by interacting with the cytoskeletal network found in their cell bodies and foot processes. (embl-heidelberg.de)
  • Here we use a battery of spectroscopic methods that track different catalytic elements of the kinase domain to show that the ~100 fold activation of the mitotic kinase Aurora A (AurA) by phosphorylation occurs without a population shift from the DFG-Out to the DFG-In state, and that the activation loop of the activated kinase remains highly dynamic. (elifesciences.org)
  • The resulting peptide/MHC complexes (p/MHC) are further trafficked through the Golgi and eventually displayed on the cell surface, where they are surveilled by CD8+ cytotoxic T cells (CTLs) through specific interactions with αβ T cell receptors (TCRs) ( 3 ). (frontiersin.org)
  • The deaminase motifs (I-III) consist of four or five highly conserved amino acids surrounding each of the cytosines and histidines that bind zinc. (biomedcentral.com)
  • Earlier, several nSH3 binding motifs were identified in the PR domain. (nih.gov)
  • Ahmed AH, Ptak CP, Fenwick MK, Hsieh CL, Weiland GA, Oswald RE, " Dynamics of cleft closure of the GluA2 ligand-binding domain in the presence of full and partial agonists revealed by hydrogen-deuterium exchange ," J Biol Chem. (cornell.edu)
  • The ADAR1 gene was found to encode three double-stranded-RNA binding domains (dsRBDs) and a deaminase domain with zinc-binding motifs (Figure 1 ), similar to those of zinc-dependent cytosine deaminases and to the cytosine-to-uracil RNA-editing enzyme APOBEC1. (biomedcentral.com)
  • Transcriptomic analysis of leaves, flowers and roots revealed 14 unique jasmintide precursors, all of which possess a three-domain architecture comprising a signal peptide, a pro-domain and a mature jasmintide domain. (biomedcentral.com)
  • The interaction of MHC-peptide complexes on the surface of an antigen presenting cell with receptors on T cells induces characteristic T cell effector functions. (umassmed.edu)
  • Subsequently, we were able to control the selectivity of the inhibitor by simple modifications and turned it into a highly selective BRD9 inhibitor. (uni-freiburg.de)
  • In contrast to large differences in the predicted gene composition of BV genomes, sequences that appear to mediate processes of viral segment formation, such as proviral segment excision and circularization, appear to be highly conserved, supporting the hypothesis of a single origin for BVs. (biomedcentral.com)
  • The 5' RNA methyltransferase activity should be located on the N-terminal portion because of the presence of conserved motifs found in other viral methyltransferases [ 5 - 7 ]. (biomedcentral.com)
  • The display of high affinity peptides expressed exclusively by the tumor (i.e., neoepitopes) on MHC molecules can elicit specific CTL responses, which forms the basis of several established immunotherapies against cancers ( 5 , 6 ). (frontiersin.org)
  • Characterization of a novel pH-sensitive peptide that enhances drug release from folate-targeted liposomes at endosomal pHs. (forexassessment.com)
  • It is mediated via a mechanism of ATP-binding-site alpha helix motif. (wikipedia.org)
  • Cancer immunotherapy harnesses a patient's CD4+ and CD8+ T cell responses toward peptide neoantigens, which are displayed on the surface of tumor cells by major histocompatibility complex molecules [MHC, termed human leukocyte antigen (HLA) in humans] ( 1 ). (frontiersin.org)
  • About exchange of certain responses thus activate SOX2 and particularly 90 Complement of these also fuel NANOG( Boyer et al. (evakoch.com)
  • A zinc finger motif near the amino terminus of the ARF1 GAP was required for stimulation of GTP hydrolysis. (embl.de)
  • The N-terminal subdomain includes Walker A and Walker B motifs which function in ATP binding and hydrolysis. (nih.gov)
  • A number of studies have demonstrated the critical function of SP-B or specific SP-B peptide sequences in pulmonary surfactant, particularly the highly conserved amino- and carboxyl-terminal sequences consisting of a repeating arginine-lysine (R-L) motif (RL4). (medscape.com)
  • Using a motif-based search strategy for antiviral targets we identified caveolin-1 (Cav-1) as a putative cellular interaction partner of human influenza A viruses, including the pandemic influenza A virus (H1N1) strains of swine origin circulating from spring 2009 on. (biomedcentral.com)
  • In cellular studies, we have discovered a new extracellular pathway for antigen presentation particularly active in immature dendritic cells, in which peptide generation and MHC loading occur entirely outside of the cell. (umassmed.edu)
  • Silent information regulator 1 (SIRT1), a highly conserved NAD + -dependent deacetylase, is a cellular regulator that has received extensive attention in recent years and regarded as a sensor of cellular energy and metabolism. (ijbs.com)
  • When bound, GTP is hydrolyzed, and cleavage of the γ-phosphate induces conformational changes leading to movements of conserved DLP domains relative to each other. (nature.com)
  • The first positive experience with a malaria vaccine in the field was with SPf66, a synthetic multiepitope, multistage peptide vaccine. (medscape.com)
  • TrkC coupling with NT3 causes preferential activation of the PI3/AKT pathway preventing apoptosis and increasing cell survival, whereas TrkB transduces the BDNF signal via Ras-ERK, PI3K and PLCγ pathway, resulting in neuronal differentiation and survival. (eonco.info)
  • Together, this study expands the existing library of jasmintides and furthers our understanding of the molecular diversity and cystine framework of CRPs as scaffolds and tools for engineering peptides targeting pests. (biomedcentral.com)
  • There is a specific motif that is used to clarify this particular family. (wikipedia.org)
  • Distinct TF motifs render enhancers more resistant or sensitive to specific CoRs, as we demonstrate by motif mutagenesis and addition. (bvsalud.org)
  • Because moesin was shown to be highly expressed in growth cones and moesin/radixin suppression led to impaired structure and function of this key element in brain development, we tested the ERM family, ezrin, radixin, and moesin, in fetal Down syndrome (DS) cortex at the early second trimester. (embl-heidelberg.de)
  • Nucleophosmin (NPM), also known as B23, 1 NO38, and numatrin, is an abundant, highly conserved, ubiquitously expressed nucleolar phosphoprotein which belongs to the nucleoplasmin/nucleophosmin family of nuclear chaperones. (haematologica.org)
  • Currently, the RTS,S/AS02 vaccine, a CSP fragment covering the central repeat peptide and the C-terminal T-cell epitope fused to the hepatitis B surface antigen formulated in the adjuvant AS02A, an oil-in-water emulsion plus monophosphoryl lipid A (GSK) plus saponin-derived QS21 (Antigenics), is undoubtedly the most advanced and promising vaccine candidate. (medscape.com)
  • ADAR3 is very well conserved in fish genomes, even though no function has been ascribed to it, suggesting that it is active on an unknown substrate. (biomedcentral.com)
  • In this work, we report that most enhancers in Drosophila can be repressed by only a subset of CoRs, and enhancers classified by CoR sensitivity show distinct chromatin features, function, TF motifs, and binding. (bvsalud.org)
  • The download Dis help triggers compromised upon deficiency of the autonomic gefitinib membrane by the inhibitor of Na+ and snRNA of K+ from the functionality, Mg2+ encodes found from the NMDA renewal can highly limit increased by the conditions. (erik-mill.de)
  • More recently, we characterized by nuclear magnetic resonance and replica exchange simulations possible cSH3 binding regions. (nih.gov)
  • Through this process, a large and heterogeneous pool of p/MHC antigens is continuously generated in healthy, pathogen infected, or tumor cells as a means of displaying a cell's peptide repertoire to the immune system ( 4 ). (frontiersin.org)
  • An ORIP staff member recently participated in a KOMP2 meeting, which was held to monitor the project's overall progress, foster exchange of scientific knowledge with international collaborators, facilitate discussions with production and phenotyping center representatives, conduct an in-depth review of data, and assess the progress and the impact of the projects. (nih.gov)
  • Native autoprocessing uses cholesterol as a substrate nucleophile to assist in cleaving an internal peptide bond within a precursor form of Hh. (bvsalud.org)
  • In preclinical studies, LSPs, coding for the C-terminal 282-383 region of the CSP were highly immunogenic and protective. (medscape.com)
  • It was postulated previously and confirmed recently that podocalyxin, a sialomucin, plays a major role in maintaining the urinary space open by virtue of the physicochemical properties of its highly negatively charged ectodomain. (embl-heidelberg.de)