Peptide transporter. Medical search. Frequent questions

Peptide transporter 1 (PepT 1) also known as solute carrier family 15 member 1 (SLC15A1) is a protein that in humans is encoded by SLC15A1 gene. (wikipedia.org)
Although a member of the SLC15 family, this protein has adapted to recognise nitrate in place of peptides, yet still retains much of the proton coupling machinary. (newsteadgroup.org)
Bailey, PD & Collier, ID 1998, ' The Synthesis of Peptide Isosteres: Their Role as Active Site Probes for the Transmembrane Peptide Transporter Protein PepT1 ', Paper presented at Peptides 96, United Kingdom, 1/01/98 pp. 229-230. (hw.ac.uk)
However, the limited set of chromatophore-encoded solute transporters appears insufficient for supporting metabolic connectivity or protein import. (frontiersin.org)
Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)
The ABCG2 multidrug transporter or abc subfamily G member 2 is a membrane protein from the A TP- B inding C assette (ABC) transporter family, specifically the G-subfamily. (proteopedia.org)
The TAP1 and TAP2 proteins attach (bind) together to form a protein complex called transporter associated with antigen processing (TAP) complex. (medlineplus.gov)
moves (transports) protein fragments (peptides) from foreign invaders into the endoplasmic reticulum. (medlineplus.gov)
Using cellular disease models for ADTKD-SEC61A1, we identified an impaired protein transport of the renal secretory protein renin and a reduced abundance of regulatory calcium transporters, including SERCA2. (amrita.edu)
Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. (lu.se)
Expression of TAP1 is down-regulated by human Epstein-Barr virus vil-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. (lu.se)
eLife, 2016) implied that the N-terminus of the magnesium transporter A (MgtA) protein (from hereon called Keif) is intrinsically disordered, but the advantage of this disordered feature to the function of the protein is still unknown. (lu.se)

Model of the mammalian peptide transporters interacting with the intestinal protease trypsin. (newsteadgroup.org)

The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
Eggensperger S, Tampe R. The transporter associated with antigen processing: a key player in adaptive immunity. (medlineplus.gov)

The objective of the current study was to examine the kinetics of amoxicillin and cefaclor interactions with human renal transporters human organic anion transporter 1 (hOAT1), human peptide transporter 1 (hPepT1), and human peptide transporter 2 (hPepT2) in detail, both as substrates and as inhibitors. (aspetjournals.org)
Cochrane Abstracts , Evidence Central , evidence.unboundmedicine.com/evidence/view/Cochrane/433293/all/Dipeptidyl_peptidase��4_inhibitors__glucagon��like_peptide_1_receptor_agonists_and_sodium��glucose_co��transporter��2_inhibitors_for_people_with_cardiovascular_disease:_a_network_meta��analysis_New. (unboundmedicine.com)
Novel types of anti-hyperglycaemic medications such as sodium glucose cotransporters-2 inhibitors, glucagon like peptide-1 receptor agonists and dipeptidyl peptidase 4 inhibitors have shown to provide potent anti-hyperglycaemic effects, but their influences on diabetes-induced apoptotic injuries is largely unknown. (nih.gov)
The uptake of small peptides by rat AM was evaluated using fluorescein isothiocyanate (FITC)-labeled (*) peptides (Arg-Lys*, Gly-Sar-Lys*, and beta-Ala-Lys*), high-performance liquid chromatography (HPLC) analysis of potential peptide degradation, and known inhibitors of Arg and PepT1 transport. (cdc.gov)
NO production by AM through Arg and Arg-containing peptides was studied with and without inhibition by transport inhibitors. (cdc.gov)
The NO production by AM through Arg-containing peptides was significantly blocked only by PepT1 inhibitors and by an anti-PepT1 antibody in a dose-dependent manner. (cdc.gov)
These structures also illustrated the transporter cycle of ABCG2, the binding locations for inhibitors, and the link between cancer and the ABC transporter family. (proteopedia.org)
At the top of the list, I would put the glucagon-like peptide 1 (GLP-1) receptor agonists, the sodium-glucose co-transporter 2 (SGLT2) inhibitors, and pioglitazone . (medscape.com)
glucosidase inhibitors, glucagon-like peptide-1 receptor agonists, dipeptidyl peptidase-IV inhibitors, sodium glucose co-transporter 2 inhibitors, bromocriptine, and bile acid sequestrants. (cdc.gov)

Synthetic peptides corresponding to SLC2A8 (solute carrier family 2, facilitated glucose transporter, member 8). (novusbio.com)

Erratum: An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins. (missouri.edu)
These proteins show similar domain architectures as known organelle-targeted expression regulators of the octotrico peptide repeat type in algae and plants. (frontiersin.org)
The synthesis and incorporation of these compounds into synthetic peptides is a key step in developing model systems with which to investigate AGE-modified proteins. (lookchem.com)
[2] ABCG2 belongs to the family of 48 transporter proteins called ATP-binding cassette transporters (ABC transporters). (proteopedia.org)
There, the peptides are attached to MHC class I proteins. (medlineplus.gov)
Because there are no peptides for MHC class I proteins to bind, they are broken down, which results in a shortage of MHC class I proteins on the surface of cells. (medlineplus.gov)
Mutations in another gene involved in the attachment of peptides to MHC class I proteins very rarely cause BLS I. (medlineplus.gov)

The attachment of amino acids or small peptides to drug molecules creates a pro drug, which is then recognized by PepT1 and PepT2 and transported into the body following oral administration. (newsteadgroup.org)
The potential of utilizing PepT1 and PepT2 as universal drug delivery and retention systems represents a profound and transformative new development in transporter mediated drug delivery. (newsteadgroup.org)
To demonstrate that rat alveolar macrophages (AM) exhibited the PepT1-like transporter for the uptake of arginine (Arg)-containing small peptides and utilized these peptides as direct substrates for nitric oxide (NO) production. (cdc.gov)
The presence of PepT1-like transporter on AM was evaluated using anti-PepT1 antisera and Western blot analysis. (cdc.gov)
The uptake of Arg-Lys*, beta-Ala-Lys*, and Gly-Sar-Lys* was blocked (approximately 50%) by cephradine (an inhibitor of PepT1 for peptide transport) but not by Lys (an inhibitor on cationic amino acid transporter 2B for Arg transport). (cdc.gov)
The presence of a PepT1-like transporter on AM was confirmed by Western blotting. (cdc.gov)
This study shows that AM exhibit PepT1-like transporter for small peptide uptake. (cdc.gov)
Arginine-containing peptides, through the PepT1 transporter system, can serve as direct substrates of iNOS for the production of NO by AM. (cdc.gov)

The human Intestinal peptide transporter hPEPT1 was transiently expressed in HeLa cells. (lookchem.com)
In hPEPT1-transfected cells but not in cells transfected with empty vector, the uptake of [14C]glycylsarcosine was strongly inhibited by alanylpyrraline (Ala-Pyrr) and pyrralylalanine (Pyrr-Ala). Free pyrraline did not inhibit peptide uptake. (lookchem.com)

Peptide transporter 1 functions in nutrient and drug transport have been studied using intestinal organoids. (wikipedia.org)
Human intestinal H+/peptide cotransporter. (wikipedia.org)
Glucagon-like peptide-2 (GLP-2) stimulates small intestinal growth through induction of intestinal epithelial proliferation. (nih.gov)

But, the production of NO by AM using these peptides as substrates was 2-fold higher than using Arg as a substrate. (cdc.gov)
One molecule of ATP is hydrolyzed to transport substrates across the cell membrane while the second molecule of ATP is hydrolyzed to reset the transporter to its inward-facing conformation. (proteopedia.org)
The overall shift from inward-facing to outward-facing promotes the transport of substrates through the transporter. (proteopedia.org)
After substrates bind in Cavity 1, ATP binds each NBD leading to the transporter shifting from inward-facing to outward-facing. (proteopedia.org)

Among the main mechanisms of this multidrug resistance is the overexpression of ATP-binding cassette (ABC) transporters that mediate drug efflux, and, specifically, ABCB1, ABCG2 and ABCC1 are known to cause cancer chemoresistance. (nature.com)
Revisiting the role of ABC transporters in multidrug-resistant cancer. (nature.com)

We are continuing to discover more details concerning the molecular basis for proton coupled peptide transport. (newsteadgroup.org)
Previous studies include understanding the role of water wires in facilitating proton movement within the binding site and our discovery that certain members of the family can transport peptides using variable proton stoichiometries , which may help to explain why peptide transport in eukaryotes is still proton coupled. (newsteadgroup.org)
Carnosine supplementation enhanced the bioavailability of carnosine in the ischemic limb, which was accompanied by increased expression of proton-coupled oligopeptide transporters. (frontiersin.org)

We did simultaneous introduction of two tumor suppressor peptides (p14 ARF and p16 INK4a or p16 INK4a and p21 CIP1 functional peptides) compared with single-peptide introduction using Wr-T-mediated peptide delivery. (aacrjournals.org)
95% within the first 72 h, whereas the growth inhibition was ∼40% by p14 or p16 single-peptide introduction. (aacrjournals.org)
which are fused together as a single peptide chain. (proteopedia.org)

Oral or duodenal administration of glucose or maltose did not reveal any differences in the ability of GLP-2-treated mice to absorb these nutrients, possibly because of decreases in expression of the glucose transporters sodium-dependent glucose transporter-1 (SGLT-1) and GLUT-2. (nih.gov)
Western Blot: Glucose Transporter GLUT8 Antibody [NBP1-59812] - Human Fetal Heart, Antibody Dilution: 1.0 ug/ml. (novusbio.com)
Western Blot: Glucose Transporter GLUT8 Antibody [NBP1-59812] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml. (novusbio.com)
SLC2A8 is the insulin-regulated facilitative glucose transporter.SLC2A8 binds cytochalasin B in a glucose-inhibitable manner.SLC2A8 seems to be a dual-specific sugar transporter as it is inhibitable by fructose. (novusbio.com)
SGLT2=sodium-glucose co-transporter-2. (medscape.com)
It is at that level that these guidelines are a bit different, [ 1 ] because we now include the sodium-glucose co-transporter 2 (SGLT2) inhibitor class at that step. (medscape.com)

Synthetic peptide. (abcam.com)

3 H-Labeled peptides eluted from B27 molecules of lymphoblasts from rats of two cim b and three cim a RT1 haplotypes showed that the cim b peptide pool favors comparatively longer and/or more hydrophobic peptides. (elsevierpure.com)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)

In this Review, we highlight the progress achieved in the past 5 years on the three transporters, ABCB1, ABCG2 and ABCC1, that are known to be of clinical importance. (nature.com)
The NBDs in ABCG2 remain in contact with one another even without a bound substrate, providing greater substrate specificity as the entrance to the transporter is not as globular as other ABC transporters like ABCB1 or ABCC1. (proteopedia.org)

A key aim of research in the group is to provide novel insights into the molecular details underpinning peptide and drug recognition within the SLC15 family and use the insights gained to develop novel strategies for drug and prodrug delivery. (newsteadgroup.org)
To understand the molecular basis for substrate recognition we also determined the crystal structure of the plant nitrate transporter, NRT1.1 . (newsteadgroup.org)
We address the molecular basis of their broad substrate specificity gleaned from structural information and discuss novel approaches to block the function of ABC transporters. (nature.com)
Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. (lu.se)
Thus, in this study, the structure of the Keif peptide part have been investigated by using both atomisic molecular dynamics. (lu.se)

The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. (elifesciences.org)

A high percentage of the ABC family transporters (19 of the 48) transport chemotherapeutic agents out of cells, making expression levels of ABC transporters a major indicator of cancer treatment prognosis. (proteopedia.org)

As an ABC Transporter , ABCG2 exhibits ATPase activity and uses the energy of ATP hydrolysis to facilitate transport. (proteopedia.org)

Peptide transporters are the major route of dietary peptide absorption in the small intestine and kidneys. (newsteadgroup.org)

Recently we determined the cryo-EM structure of PepT2 , revealing the mechanism for peptide and prodrug recognition. (newsteadgroup.org)

Fig. 2: Structures of three ATP-binding cassette transporters. (nature.com)
Fig. 3: Substrate-binding pockets of ATP-binding cassette transporters and the drug transport cycle. (nature.com)
Fig. 4: Emerging therapies targeting ATP-binding cassette transporters. (nature.com)

Furthermore, genetic modification of ABC transporters by CRISPR-Cas9 and approaches to re-engineer amino acid sequences to change the direction of transport from efflux to import are briefly discussed. (nature.com)

Previous studies have suggested the involvement of organic anion and oligopeptide transporters in membrane transport of β-lactams. (aspetjournals.org)
Rats transgenic for the human MHC molecule HLA-B27 were used to study the effect of two alleles, cim a and cim b , which are associated with peptide transport by the MHC-encoded Tap2 transporter, on the function of HLA-B27 as a restriction element for CTL recognition of the male H-Y minor H Ag and on the multisystem inflammatory disease characteristic of B27 transgenic rats. (elsevierpure.com)
Transport and utilization of arginine and arginine-containing peptides by rat alveolar macrophages. (cdc.gov)
High-resolution structures, biophysical and in silico studies have led to tremendous progress in understanding the mechanism of drug transport by these ABC transporters, and several promising therapies, including irradiation-based immune and thermal therapies, and nanomedicine have been used to overcome ABC transporter-mediated cancer chemoresistance. (nature.com)
Mutations in the TAP1 or TAP2 gene prevent formation of the TAP complex, which impairs the transport of peptides into the endoplasmic reticulum. (medlineplus.gov)

Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. (elifesciences.org)
We discuss using the combination of basal insulin plus a glucagon -like peptide-1 (GLP-1) receptor agonist and, potentially, also the combination of insulin plus an SGLT2 inhibitor. (medscape.com)

peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
The availability of Arg-containing peptides in the lung was determined by HPLC analysis of bronchoalveolar lavage (BAL) fluid. (cdc.gov)

1998. 229-230 Paper presented at Peptides 96, United Kingdom. (hw.ac.uk)

Atrial natriuretic peptide (ANP) decreases plasma Na concentration and promtes seawater (SW) adaptation in eels. (bioone.org)

The synthesis of advanced glycation endproducts (AGEs), CML, CEL, and pyrraline and their incorporation into collagen model peptides is reported. (lookchem.com)

Mutation screening of two candidate genes from 13q32 in families affected with Bipolar disorder: human peptide transporter (SLC15A1) and human glypican5 (GPC5). (uchicago.edu)
Here, we examine growth suppression efficiency of human glioblastomas by dual-peptide targeting. (aacrjournals.org)
Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. (lu.se)

Since determining the first crystal structure of a peptide transporter in 2011 we have continued to expand our understanding of this important SLC family, recently reviewed here . (newsteadgroup.org)
Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. (elifesciences.org)

For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)

Functional peptides are among the more desirable of these nanobio-tools. (aacrjournals.org)
Intracellular delivery of multiple functional peptides forms a basis for potent, non-invasive mode of delivery, providing distinctive therapeutic advantages. (aacrjournals.org)

Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)

Belongs to the abc transporter family. (lu.se)

Our current work is focused on drug complexes and the recognition of xenobiotics in the mammalian transporters. (newsteadgroup.org)
Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)
the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)

The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
The FITC-labeled peptides were internalized by AM without degradation. (cdc.gov)

When these immune system cells recognize the peptides as harmful, they launch an immune response to get rid of the foreign invaders. (medlineplus.gov)

This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)
In this issue of the JCI , two separate studies on MHC-bound peptides reopen the debate on the utility of peptides for the purposes of vaccination and treatment of autoimmune diseases. (jci.org)

We suggest that current information regarding the structure, mechanism and regulation of ABC transporters should be used in clinical trials to improve the efficiency of chemotherapeutics for patients with cancer. (nature.com)

Anatomy19

Organisms15

Chemicals and Drugs151

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes45

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science4

Protein12

Mammalian peptide transporters1

Antigen3

Inhibitors9

Solute1

Proteins7

PepT18

HPepT12

Intestinal3

Substrates4

Multidrug2

Proton coupled3

Single-peptide3

Glucose6

Synthetic1

Molecules2

ABCB12

Molecular5

Affinity1

Expression1

ABCG21

Absorption1

PepT21

Cassette3

Amino acid1

Transport5

Receptor2

Bronchoalveolar lavage2

19981

Decreases1

Synthesis1

Human3

Crystal2

Inflammatory1

Functional2

HPLC1

Family1

Recognition3

Degradation2

Immune1

Bound2

Regulation1