• We have previously determined the structure of the MHC class I molecule HLA-A1 bound to a melanoma antigen-encoding gene (MAGE)-A1-derived peptide in complex with a recombinant antibody fragment with TCR-like specificity, Fab-Hyb3. (rcsb.org)
  • Antigen Peptide CMV IE-1 - HLA-A*0301 (KLGGALQAK) for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxity or proliferation assays. (jpt.com)
  • Find your antigen peptide and select the connected PepMix™ Peptide Pool and MHC Multimer for efficient immune monitoring, mapping of T cell epitopes or development of immunotherapy and vaccines. (jpt.com)
  • Human leukocyte antigen (HLA) class I allotypes vary in their ability to present peptides in the absence of tapasin, an essential component of the peptide loading complex. (ox.ac.uk)
  • HLA class I histocompatibility antigen, alpha chain F is a protein that in humans is encoded by the HLA-F gene. (wikipedia.org)
  • The Major Histocompatibility Complex (MHC) is a group of cell surface proteins that in humans is also called the Human Leukocyte Antigen (HLA) complex. (wikipedia.org)
  • The human leukocyte antigen (HLA) proteins are an indispensable component of adaptive immunity because of their role in presenting self and foreign peptides to T cells. (genes-environment-inflammation.de)
  • The protein is called human leukocyte antigen B27 (HLA-B27). (medlineplus.gov)
  • The molecular basis for peptide repertoire selection in the human leucocyte antigen (HLA) C*06:02 molecule. (bvsalud.org)
  • Human leukocyte antigen (HLA)-C*0602 is identified as the allele associated with the highest risk for the development of the autoimmune skin disease psoriasis . (bvsalud.org)
  • The ADAMTSL5 autoantigen possessed a P7-Leu instead of the P7-Arg residue, but nevertheless was accommodated within the HLA-C *0602 antigen -binding cleft. (bvsalud.org)
  • Furthermore, genetic associations including the human leucocyte antigen (HLA) DRB1*15:01-DQB1*06:02 haplotype influence susceptibility. (liverpool.ac.uk)
  • This study aimed to (1) investigate whether amoxicillin-modified HLA-DRB1*15:01-DQB1*06:02 binding peptides selectively activate DILI patient T cells and (2) define the nature of the T-cell response with respective to antigen structure. (liverpool.ac.uk)
  • The processing and presentation of HLA-B7 in these cells appears to be through the endogenous, and not the exogenous, pathway of antigen presentation. (silverchair.com)
  • Direct visualisation of hepatitis B virus (HBV)-specific cytotoxic T cells with an human leucocyte antigen (HLA) class I peptide tetrameric complex allows a new insight into their actual frequency. (jenner.ac.uk)
  • Here, immunogenic potential of neoantigen-peptides of tumor tissues and matched organoids ( n = 27 pairs) are analyzed by Score tools with whole genome sequencing (WGS)-based human leukocyte antigen (HLA)-class-I algorithms. (bioinfor.com)
  • In addition, we have proposed a model in which the almost instantaneous processing of cytosolic antigens is not due to defectiveness on the part of the antigen but, rather, unchaperoned nascent peptide. (upenn.edu)
  • 2. The cell biology of MHC class II-restricted antigen processing and presentation: A generally accepted paradigm in fundamental immunology is that CD4+ T cells recognize peptides derived from exogenous (internalized) sources of antigen while CD8+ recognize peptides derived from endogenous sources of antigen (generally, proteins synthesized within the antigen-presenting cell). (upenn.edu)
  • I have had a longstanding interest in MHC class II-bound peptides derived from endogenous sources of antigen. (upenn.edu)
  • No amount of exogenous, inactivated virus, displaying abundant amounts of NA at the virion surface, could be converted to class II-bound peptide by antigen-presenting B cells. (upenn.edu)
  • The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
  • Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
  • To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. (jci.org)
  • However, we recently identified a multiple myeloma-reactive T-cell receptor (TCR) that appeared to recognize antigen presented on HLA-C*04:09N and encouraged us to ask whether HLA-C*04:09N, albeit not easily detectable at the cell surface, can present antigen sufficient for T-cell activation. (haematologica.org)
  • The first human leukocyte antigen (HLA) haplotype association with human inflammatory disease was discovered in 1972, correlating HLA-B27 with ankylosing spondylitis. (medscape.com)
  • Class I MHC molecules include HLA-A, HLA-B, or HLA-C and serve as the antigen-presenting platform for CD8 or suppressor T cells. (medscape.com)
  • Class II MHC molecules, the HLA-D region, serve as the antigen-presenting cells for CD4 or helper T cells. (medscape.com)
  • The human leukocyte antigen-B27 (HLA-B27) allele is more common in this form of JIA. (msdmanuals.com)
  • Dear Netters, I'm looking for software that determines whether peptides from a given primary amino acid sequence are likely candidates for binding to the peptide-binding groove of HLA class I (or class II) molecules. (bio.net)
  • Therefore, conformational changes within the HLA-A1:MAGE-A1:Fab-Hyb3 complex include MHC residues that are also involved in reorientations in complexes with natural ligands, pointing to their central importance for the peptide-dependent recognition of MHC molecules. (rcsb.org)
  • The N and C termini of peptides presented by major histocompatibility complex (MHC) class I molecules are held within the peptide binding groove by a network of hydrogen bonds to conserved MHC residues. (rcsb.org)
  • A comparison of HLA-B*3501/peptide complexes with structures of other HLA molecules exhibits allele-specific properties of HLA-B*3501, as well as peptide-induced structural changes. (rcsb.org)
  • Its conformation rather resembles that of a deca-peptide than of other nona-peptides bound to class I molecules. (rcsb.org)
  • Support for this pattern came from sequencing peptides eluted from purified HLA B8 molecules, where lysines were predominant at positions 3 and 5. (ox.ac.uk)
  • However, the F-pocket of HLA-F appears well conserved with HLA-E and the other class Ia molecules and likely favors an aliphatic group, such as leucine. (wikipedia.org)
  • Classic HLA class I molecules interact with HLA-F through their heavy chain. (wikipedia.org)
  • However, HLA class I molecules only interact with HLA-F when they are in the form of an open conformer (free of peptide). (wikipedia.org)
  • In the cellular immune response, recognition by CTL-TCRs of viral antigens presented as peptides by HLA class I molecules, triggers destruction of the virally infected cell (Townsend, A.R.M., J. Rothbard, F.M. Gotch, G. Bahadur, D. Wraith, and A.J. McMichael. (ox.ac.uk)
  • Class I MHC molecules bind peptides in the endoplasmic reticulum and present them at the cell surface to circulating CD8+ T cells for analysis. (ox.ac.uk)
  • We have examined binding of peptides and stabilization of HLA-Aw68 class I molecules using synthetic peptide variants of an influenza virus nucleoprotein peptide, NP91-99 (KTGGPIYKR). (ox.ac.uk)
  • Human leukocytes were stimulated in vitro with peptides corresponding in sequence to the highly variable helix of the alpha 1 domain of various HLA-B and -C molecules. (silverchair.com)
  • The HLA-DR11.2 molecule, which only differs at three residues from HLA-DR11.1, did not present the HLA-B7 peptide to CTL-AV. Peptides from the alpha 1 domain helix of other HLA-A and HLA-B molecules, but not HLA-C molecules, competed with the HLA-B7 peptide for binding to HLA-DR11.1. (silverchair.com)
  • Furthermore, introduction of purified HLA-B7 molecules into HLA-DR11.1+, HLA-B7- cells by cytoplasmic loading via osmotic lysis of pinosomes, but not by simple incubation, rendered them susceptible to CTL-AV killing. (silverchair.com)
  • They also emphasize the capacity for presentation of MHC peptides by MHC molecules. (silverchair.com)
  • 3 H-Labeled peptides eluted from B27 molecules of lymphoblasts from rats of two cim b and three cim a RT1 haplotypes showed that the cim b peptide pool favors comparatively longer and/or more hydrophobic peptides. (elsevierpure.com)
  • Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity. (ucytech.com)
  • The peptides from Mycobacterium tuberculosis (Mtb) potentially presented by HLA-E molecules, however, are unknown. (ucytech.com)
  • Synthetic peptides that inhibit binding of the myelin basic protein 85-99 epitope to multiple sclerosis-associated HLA-DR2 molecules and MBP-specific T-cell responses. (ox.ac.uk)
  • In the present study various peptides, synthesized according to the binding motifs for both the immunodominant epitope of myelin basic protein (MBP) 85-99, a candidate autoantigen in MS, and Cop 1, differentially inhibited binding of these antigens to disease-associated HLA-DR2 (DRB1*1501) molecules. (ox.ac.uk)
  • In particular, two peptides with residue K at position P-1, as referred to MBP 85-99, inhibited effectively the binding of both biotinylated MBP 85-99 and Cop 1 to HLA-DR2 molecules as well as IL-2 production by two MBP-specific HLA-DR2-restricted T-cell clones. (ox.ac.uk)
  • HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
  • Autoimmune/inflammatory conditions can occur if mutations in the groove binding site of class I and II molecules occur, leading to inappropriate binding to self-peptides or certain environmental peptides. (medscape.com)
  • peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
  • Immuno-informatic analysis predicted that the identified T-cell antigens (AgA and AgD) bind more number of class I and class II HLA alleles as compared with the reference immuno-dominant antigens ESAT-6 and CFP-10 from the tuberculous mycobacterial species M. tuberculosis. (cdc.gov)
  • These two antigens were further predicted to be highly immunogenic for class I peptide MHC (pMHC) complex as compared to the reference antigens. (cdc.gov)
  • Collectively, our results imply that AgA and AgD are T-cell antigens with a high HLA binding frequency as well as population coverage for HLA alleles. (cdc.gov)
  • A new nomenclature system for HLA antigens was recently devised by the WHO Nomenclature Committee for Factors of the HLA System. (bvsalud.org)
  • This system was needed to support the vast number of HLA antigens that are now characterized at the level of DNA sequence. (bvsalud.org)
  • See Nomenclature for Factors of the HLA System for further information on how HLA antigens are named. (bvsalud.org)
  • Here, we compare the X-ray structure of HLA-A1:MAGE-A1 with that complexed with Fab-Hyb3 to gain insight into structural changes of the MHC molecule that might be induced by the interaction with the antibody fragment. (rcsb.org)
  • Taken together, these data illustrate the strong interdependence between the HLA class I molecule and the bound peptide. (rcsb.org)
  • A model was made of the HLA B8 molecule and negatively charged pockets predicted, which could accommodate the positively charged side chains of the peptide anchor residues. (ox.ac.uk)
  • On healthy cells the non-classical HLA class Ib molecule HLA-E displays the cognate ligand for the NK cell receptor NKG2A/CD94 when bound to HLA class I signal peptide sequences. (mpg.de)
  • The activation of CD8⁺ T cells by certain p:HLA-E complexes illustrates the dual role of this low polymorphic HLA molecule in innate and adaptive immunity. (mpg.de)
  • The HLA-F protein is a ~40-41 kDa molecule with conserved domains. (wikipedia.org)
  • Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. (nih.gov)
  • However, the diversity and mode of peptide presentation by the HLA-C *0602 molecule remains unclear. (bvsalud.org)
  • Cytotoxic T cell recognition of an endogenous class I HLA peptide presented by a class II HLA molecule. (silverchair.com)
  • Rats transgenic for the human MHC molecule HLA-B27 were used to study the effect of two alleles, cim a and cim b , which are associated with peptide transport by the MHC-encoded Tap2 transporter, on the function of HLA-B27 as a restriction element for CTL recognition of the male H-Y minor H Ag and on the multisystem inflammatory disease characteristic of B27 transgenic rats. (elsevierpure.com)
  • A sequence pattern for peptides presented to cytotoxic T lymphocytes by HLA B8 revealed by analysis of epitopes and eluted peptides. (ox.ac.uk)
  • Additionally, we analyzed the recognition of these p:HLA-E epitopes by CD8⁺ T cells. (mpg.de)
  • These novel HLA-E epitopes provide new implications for therapies targeting cells with abnormal HLA class I expression. (mpg.de)
  • Prominent, solvent-exposed structures within these peptides may participate in generating T cell responses to these immunodominant epitopes. (ox.ac.uk)
  • We have previously mapped four peptides containing immunodominant T-helper cell epitopes from the RhD protein and the purpose of the work was to develop these into a product for suppression of established anti-D responses. (elsevierpure.com)
  • We used a novel in vitro degradation assay involving cytosolic extracts as well as endogenous intracellular processing assays to examine 2 well-characterized HIV-1 Gag overlapping epitopes presented by the same HLA class I allele, one of which is consistently immunodominant and the other subdominant in infected persons. (jci.org)
  • the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
  • The peptide is synthesised as it is presented by the MHC class I HLA-A*2402 allele. (neuromics.com)
  • HLA-F Allele-Specific Peptide Restriction Represents an Exceptional Proteomic Footprint. (nih.gov)
  • We have previously shown that Scandinavian sarcoidosis patients expressing the HLA-DR allele DRB1*0301 are characterized by large accumulations in the lungs of CD4+ T cells expressing the TCR AV2S3 gene segment. (jci.org)
  • The null allele HLA-C*04:09N differs from HLA-C*04:01 in a frameshift mutation within its cytoplasmic domain, resulting in translation of 32 additional amino acids that are assumed to prevent cell surface expression. (haematologica.org)
  • In conclusion, we present peptide-specific HLA-C*04:09N-restricted T-cell activation and suggest consideration of this allele in the appropriate clinical context such as allogeneic stem cell transplantation, or in the setting of cellular therapy. (haematologica.org)
  • JPT Peptide Technologies has substantial, long-standing expertise in providing peptides, peptidomimetics, and proteins to the global scientific community. (jpt.com)
  • These proteins are encoded by a cluster of genes known as the HLA locus. (wikipedia.org)
  • HLA-F appears to be a recombinant between two multigene families, one that comprises conserved sequences found in all class I proteins (single transmembrane span) and another distinct family of genes with a conserved 3' UTR. (wikipedia.org)
  • Exon 7 is absent from the mRNA of HLA-F. The absence of this exon produces a modification in the cytoplasmic tail of the protein making it shorter relative to classical HLA class-I proteins. (wikipedia.org)
  • Identifying the specific set of peptides presented by an individual's HLA proteins in vivo , as a whole being referred to as the immunopeptidome, has therefore gathered increasing attention for different reasons. (genes-environment-inflammation.de)
  • Probable anchor residues were identified at peptide residues 3 (lysine/arginine), 5 (lysine/arginine) and 9 (leucine/isoleucine). (ox.ac.uk)
  • A model epitope restricted by HLA-A2 and a series of epitope precursors with N-terminal extensions by single residues modifying TAP affinity were expressed in a system that allowed us to monitor and modulate cytosolic peptide copy numbers. (elsevierpure.com)
  • Of the key residues likely to form from the floor of the groove, position 97 is a glycine whose residue is a single proton, whereas in most class Ia structures it is a charged residue and in HLA-E, it is a bulky hydrophobic tryptophan. (wikipedia.org)
  • This nonclassical class I gene also has two histidine residues (His 114-His116) close together in the C-terminal groove floor, mirroring His-9-His99 in HLA-E. Tyr 7, Tyr 59, Tyr 159 and Tyr 171, which are typically involved in the hydrogen-bonding network to the peptide N-terminal residues, are conserved. (wikipedia.org)
  • In the HLA-B*2705 complex with flu NP383-391, the amino acid side chains of residues 4, 7 and 8 are solvent-exposed whilst in the HIV decamer, the main-chain bulges into the solvent around P7. (ox.ac.uk)
  • We found that HLA-C *0602 predominantly presents nonamer peptides with dominant arginine anchors at the P2 and P7 positions and a preference for small hydrophobic residues at the C terminus (PΩ). (bvsalud.org)
  • Thermal studies show that polyalanine peptides with minimal anchors and nearly all TCR contact residues exchanged stabilized HLA-Aw68 to high temperatures. (ox.ac.uk)
  • These data suggest that the strength of binding of peptides to HLA-Aw68 is not only dictated by the presence of anchor residues but also by the lack of unfavorable contacts between the peptide ligand and class I MHC-binding cleft. (ox.ac.uk)
  • Peptides carrying lysine residues for amoxicillin binding in positions (KP) 2-6 and anchors for the HLA-DRB1*15:01-DQB1*06:02 haplotype were designed. (liverpool.ac.uk)
  • The dominant epitope was mapped using recombinant vaccinia viruses that expressed fragments of the NP and then synthetic peptides based on the NP amino acid sequence. (ox.ac.uk)
  • The amino acid sequence of the peptide epitope was compared to six other known virus peptides known to be restricted by HLA B8 and a sequence homology was identified, which predicted nonamer and octamer epitope sequences. (ox.ac.uk)
  • One of the predicted epitope peptides was made and shown to be recognized by specific CTL. (ox.ac.uk)
  • Based on this motif, we identified an octamer epitope from influenza A matrix protein which is presented by HLA-B35. (ox.ac.uk)
  • The natural epitope contains a serine residue at P2 of the peptide. (ox.ac.uk)
  • The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
  • Thus, tapasin dependence level, like HLA zygosity, may serve as a means to restrict or expand breadth of the HLA-I peptide repertoire across humans, ultimately influencing immune responses to pathogens and vaccines. (ox.ac.uk)
  • The HLA system is genetically encoded in humans by the major histocompatibility complex (MHC), which is found on chromosome 6, and plays a determining role in immunity and in self-recognition in virtually all cells and tissues, with the exception of erythrocytes. (medscape.com)
  • MHC class I ligands are recruited from the cytosolic peptide pool, whose size is likely to depend on the balance between peptide generation by the proteasome and peptide degradation by downstream peptidases. (elsevierpure.com)
  • Altered peptide ligands (APLs) which antagonise CTL recognition of infected cells have been reported (Jameson, S.C., F.R. Carbone, and M.J. Bevan. (ox.ac.uk)
  • For example, 80-90% of patients with ankylosing spondylitis have HLA-B27. (medscape.com)
  • On the other hand, residue 145 is subject to a reorientation following engagement of HLA-Cw4 and KIR2DL1. (rcsb.org)
  • Peptides associated with HLA-B35 commonly have a proline or occasionally a serine residue in the P2 anchor position of the peptide, with a tyrosine at the C terminus. (ox.ac.uk)
  • Substitution of this residue with proline (the favored amino acid in this position in B35-associated peptides) considerably enhances binding to HLA-B35 in the T2-B35 cell line, but the peptide is not recognized by the majority of CTL clones and can antagonize recognition of the index peptide. (ox.ac.uk)
  • The tight hydrogen-bonding network observed between the HLA-B*2705 B-pocket and the peptide P2 arginine guanadinium anchor explains why mutation of this residue during HIV infection results in loss of peptide binding, immune escape and progression to AIDS. (ox.ac.uk)
  • To conclude, we show that amoxicillin-modified peptides bind to both components of the risk haplotype to stimulate DILI patient T cells and describe the importance of the position of nucleophilic lysine residue in the HLA binding peptide sequence. (liverpool.ac.uk)
  • Abstract #720: The HLA-A2 restricted HER2/neu derived peptides (E75 and GP2) are both highly immunogenic in HLA-A3 breast cancer patients vaccinated with an E75 vaccine. (aacrjournals.org)
  • Multiomics analysis combining WGS, RNA-seq, single-cell RNA-seq, mass spectrometry filters out 93 candidate neoantigen-peptides with strong immunogenic potential for functional validation in five organoids. (bioinfor.com)
  • Immunogenic peptides are defined by inducing increased CD107aCD137IFN- γ expressions and IFN- γ secretion of CD8 cells in flow cytometry and enzyme-linked immunosorbent assay assays. (bioinfor.com)
  • Nine immunogenic peptides shared by at least two individuals are validated, including peptide from TP53 R90S . (bioinfor.com)
  • Organoid killing assay confirms the antitumor activity of validated immunogenic peptide-reactive CD8 cells, which is further enhanced in the presence of immune checkpoint inhibitors. (bioinfor.com)
  • Six HLA B8-positive influenza immune donors that we have tested respond to this peptide as part of their influenza-specific CTL response. (ox.ac.uk)
  • This suggests that variation in tapasin dependence may impact the strength of the immune responses by altering peptide repertoire size. (ox.ac.uk)
  • Between innate and adaptive immune responses: NKG2A, NKG2C, and CD8⁺ T cell recognition of HLA-E restricted self-peptides acquired in the absence of HLA-Ia. (mpg.de)
  • The surface expression of HLA-F coincides with the activated immune response since HLA-F is mostly found on the surface of stimulated T memory cells but not on circulating regulatory T cells. (wikipedia.org)
  • It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. (nih.gov)
  • Our results significantly increase our understanding of the human immune response to Mtb by identification of CD8(+) T-cell responses to novel HLA-E binding peptides of Mtb, which have cytotoxic as well as immunoregulatory activity. (ucytech.com)
  • Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8+ T lymphocytes. (jci.org)
  • The comparisons between 9203 predicted neoantigen-peptides from 2449 mutations of tumor tissues and 9991 ones from 2637 mutations of matched organoids demonstrate that organoids preserved majority of genetic features, HLA alleles, and similar neoantigen landscape of original tumors. (bioinfor.com)
  • Relationship between certain HLA alleles and the risk of cytomegalovirus reactivation following allogeneic hematopoietic stem cell transplantation. (cdc.gov)
  • To determine the structural basis of this selectivity, we determined crystal structures of HLA-C *0602 in complex with two self - peptides (ARTELYRSL and ARFNDLRFV) and an analogue of a melanocyte autoantigen (ADAMTSL5, VRSRR-abu-LRL) implicated in psoriasis . (bvsalud.org)
  • it was shown that the glutamic acid at position 380 was essential for recognition by CTL and that the nonamer 380-388 was the optimum peptide. (ox.ac.uk)
  • The variant peptides cause changes in the recognition surface by three mechanisms: changes contributed directly by the peptide, effects transmitted to the exposed peptide surface, and induced effects on the exposed framework of the peptide binding groove. (ox.ac.uk)
  • Crystal structures and KIR3DL1 recognition of three immunodominant viral peptides complexed to HLA-B*2705. (ox.ac.uk)
  • Long-term non-progression during HIV infection has been associated with presentation by HLA-B*2705, and T cell recognition, of the highly immunodominant KRWIILGLNK peptide. (ox.ac.uk)
  • Extensive alanine substitutions increase binding affinity of an influenza nucleoprotein peptide to HLA-Aw68 and do not abrogate peptide-specific CTL recognition. (ox.ac.uk)
  • A panel of each of the four immunodominant RhD peptides was synthesized with modifications to improve manufacturability and solubility, and screened for retention of recognition by human T-helper cells. (elsevierpure.com)
  • Furthermore, individual p:HLA-E complexes elicit activation of CD8⁺ T cells with an effector memory phenotype. (mpg.de)
  • Of patients with uveitis, 19-88% have the HLA-B27 phenotype, depending upon the study population cited. (medscape.com)
  • Thus, the principle of protein or peptide haptenation could be used in in vitro assays to predict the sensitization potential of a new chemical entity. (cdc.gov)
  • Independent of the length of the bound peptide, HLA-B*3501 positions the peptide C terminus significantly closer to the alpha1-helix and nearer to the A pocket than observed for other HLA class I/peptide complexes. (rcsb.org)
  • In a pathogenic situation when HLA class I is absent, HLA-E is bound to a diverse set of peptides and enables the stimulatory NKG2C/CD94 receptor to bind. (mpg.de)
  • Thus, HLA-F is expressed independently of bound peptide. (wikipedia.org)
  • Tetrameric complexes of HLA-B*2705 with the HIV and flu but not EBV peptides bound strongly to the killer-Ig-like receptor (KIR)3DL1. (ox.ac.uk)
  • The T cells response was HLA class II restricted and the amoxicillin-modified peptides bound selectively to HLA-DRB1*15:01 and/or DQB1*06:02. (liverpool.ac.uk)
  • In this case, we identified by tandem mass spectrometry numerous class I-bound recoded peptides in gentamycin-treated cells but not their control counterparts. (upenn.edu)
  • For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)
  • This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)
  • In this issue of the JCI , two separate studies on MHC-bound peptides reopen the debate on the utility of peptides for the purposes of vaccination and treatment of autoimmune diseases. (jci.org)
  • Here, we describe the endogenous peptide repertoire of â ¼3,000 sequences for HLA-C *0602 that defines the peptide -binding motif for this HLA allomorph. (bvsalud.org)
  • Analysis of the peptide sequences show that the stabilization effects are not due to the hydrophobicity of the peptide. (ox.ac.uk)
  • Recently it has become possible to accurately predict spectra from peptide sequences 14 , 15 , but direct searching of single-injection DIA data has remained problematic, in part due to the false discovery rate (FDR) correction required when considering all possible tryptic peptides in a FASTA database. (nature.com)
  • We asked what fraction of this pool is available for presentation, and how the size of this fraction is modulated by peptide affinity for the TAP transporters. (elsevierpure.com)
  • Here we describe human T-cell responses to Mtb-derived peptides containing predicted HLA-E binding motifs and binding-affinity for HLA-E. We observed CD8(+) T-cell proliferation to the majority of the 69 peptides tested in Mtb responsive adults as well as in BCG-vaccinated infants. (ucytech.com)
  • HLA-F has little allelic polymorphism and is highly conserved in other primates. (wikipedia.org)
  • These results indicate that RT1-linked Tap2 polymorphism in the rat strongly influences peptide loading of HLA-B27. (elsevierpure.com)
  • It thus appears either that binding of specific peptides to B27 is unimportant in the pathogenesis of B27-associated disease or that the critical peptides, unlike H-Y and many others, are not influenced by Tap transporter polymorphism. (elsevierpure.com)
  • HLA-E restricted responses may be of interest for vaccine development since HLA-E displays very limited polymorphism (only 2 coding variants exist), and is not down-regulated by HIV-infection. (ucytech.com)
  • HLA-E restricted cytomegalovirus UL40 peptide polymorphism may represent a risk factor following congenital infection. (cdc.gov)
  • however, there have been found different isoforms from numerous transcript variants found for the HLA-F gene. (wikipedia.org)
  • These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. (nih.gov)
  • Peptide variants with three or more alanines do not show saturable binding to HLA-Aw68 and also are not recognized by the T cell line. (ox.ac.uk)
  • T-cell clones were then tested for specificity with amoxicillin, unmodified- and amoxicillin-modified peptides, and structural variants. (liverpool.ac.uk)
  • When experiment-specific spectrum library generation is either impossible or impractical, as is frequently the case with non-model organisms, sequence variants, splice isoforms, or scarce sample quantities, software tools such as Pecan 10 and DIA-Umpire 11 can detect peptides from DIA experiments without a spectrum library by directly searching every peptide in FASTA databases. (nature.com)
  • In support of this model, we observed that individuals carrying HLA class I genotypes characterized by greater tapasin independence progress more slowly to AIDS and maintain lower viral loads, presumably due to increased breadth of peptide presentation. (ox.ac.uk)
  • We recently showed that HLA-E presents a highly diverse set of peptides in the absence of HLA class Ia and revealed a non-protective feature against NK cell cytotoxicity mediated by these peptides. (mpg.de)
  • It belongs to the HLA class I heavy chain paralogues that separate from most of the HLA heavy chains. (wikipedia.org)
  • HLA-A, B, and C are the classical class I genes and HLA-E, F and G are the nonclassical class I genes. (wikipedia.org)
  • The structure of HLA-F is similar to that of the other HLA class I genes, which consist of eight exons. (wikipedia.org)
  • This gene belongs to the HLA class I heavy chain paralogues. (nih.gov)
  • The study characterizes HLA-class-I neoantigen landscape in hepatobiliary tumor, providing practical strategy with tumor organoid model for neoantigen-peptide identification in personalized immunotherapy. (bioinfor.com)
  • We generated two HLA-class I-deficient cell lines, re-expressed HLA-C*04:09N, detected HLA expression by flow cytometry, and tested for T-cell activation using a cytomegalovirus peptide-specific HLA-C*04:01-restricted TCR. (haematologica.org)
  • Recombinant HLA-C*04:09N expression was sufficient for T-cell activation in vitro , which could be blocked by an HLA-class I-specific antibody, suggesting HLA-TCR interaction at the cell surface. (haematologica.org)
  • HLA class I deficiencies due to mutations in subunit 1 of the peptide transporter TAP1. (lu.se)
  • Therefore, we developed a proteogenomic pipeline based on deep learning de novo mass spectrometry (MS) to enable the discovery of noncanonical MHC class I-associated peptides (ncMAP) from noncoding regions. (lu.se)
  • For example, identifying neoepitopes for cancer immunotherapy, vaccine development against infectious pathogens, or elucidating the role of HLA in autoimmunity. (genes-environment-inflammation.de)
  • The results support human trials of RhDPmix peptide immunotherapy in women with established antibody responses to the RhD blood group. (elsevierpure.com)
  • HLA tapasin independence: broader peptide repertoire and HIV control. (ox.ac.uk)
  • Recent studies revealed a shift in the HLA-E peptide repertoire in cells with defects in the peptide loading complex machinery. (mpg.de)
  • Collectively, our results provide the structural basis for understanding peptide repertoire selection in HLA-C *0602. (bvsalud.org)
  • Further, many complex diseases are associated with genetic variation in the HLA region, implying an important role for specific HLA-presented peptides in the etiology of these diseases. (genes-environment-inflammation.de)
  • We have examined the high resolution crystal structures of four of these APLs in complex with HLA B8 to determine alterations in the shape, chemistry, and local flexibility of the TCR binding surface. (ox.ac.uk)
  • Although there is a great decrease in the t 1/2 of the MHC/peptide complex for NP-1A compared with NP91-99, a T cell line, stimulated by NP91-99, recognizes NP-1A efficiently. (ox.ac.uk)
  • Prostate cancer disease recurrence after radical prostatectomy is associated with HLA type and local cytomegalovirus immunity. (cdc.gov)
  • HLA B8-restricted cytotoxic T lymphocytes (CTL) specific for influenza A virus were generated and shown to recognize the nucleoprotein (NP). (ox.ac.uk)
  • Copolymer 1 (Cop 1, poly [Y, E, A, K]) is a random synthetic amino acid copolymer effective in the treatment of relapsing forms of multiple sclerosis (MS), a disease that is linked to HLA-DR2 (DRB1*1501). (ox.ac.uk)
  • Due to the short distance between the N and C termini, the nona-peptide is compressed and forced to zig-zag vertically within the binding groove. (rcsb.org)
  • These structures revealed that HLA-C *0602 possesses a deep peptide -binding groove comprising two electronegative B- and E-pockets that coincide with the preference for P2 and P7 arginine anchors. (bvsalud.org)
  • This was the IMCgp100-202 (NCT03070392) study, which involved 378 patients with metastatic uveal melanoma who were HLA-A*02:01 genotype positive (identified by a central assay) who had not been previously treated. (medscape.com)
  • The amoxicillin-modified peptides were characterized by mass spectrometry prior to culturing with patient peripheral blood mononuclear cell. (liverpool.ac.uk)
  • Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)
  • Single peptide (GYQPYRVVVL) for stimulation of human SARS-CoV-2-specific CD8+ T-cells. (neuromics.com)
  • The protein encoded from the gene HLA-F was originally isolated from the human lymphoblastoid cell line 721. (wikipedia.org)
  • The cytoplasmic tail helps HLA-F exit the endoplasmic reticulum, and that function is primarily done by the amino acid valine found at the C-terminal end of the tail. (wikipedia.org)
  • After HLA-DR15 transgenic mice had been immunized with RhD protein, a single dose of RhDPmix, given either intranasally (p=0.008, Mann-Whitney rank sum test) or subcutaneously (p=0.043), rapidly and significantly suppressed the ongoing antibody response. (elsevierpure.com)
  • We show that non-canonical peptides provide stable cell surface expression of HLA-E, and these p:HLA-E complexes still bind to NKG2/CD94 receptors in a peptide-restricted fashion. (mpg.de)
  • The possible pocket regions of HLA-F include a situation where the A pocket is hydrophobic and similar to that of HLA-E, and pocket B retains Met 45 and Ala 67, which also characterize the HLA-E pocket and are likely to be hydrophobic and large. (wikipedia.org)
  • Superposition of both HLA-B*3501/peptide complexes additionally reveals a significant, peptide-dependent deviation between the N-terminal parts of the alpha1-helices which might be due to different positioning of the peptide N termini. (rcsb.org)
  • In the present study we have evaluated the molecular basis for the impaired NK cell inhibition by these peptides and determined the cell surface stability of individual p:HLA-E complexes and their binding efficiency to soluble NKG2A/CD94 or NKG2C/CD94 receptors. (mpg.de)
  • We have solved the crystal structures of three HLA-B*2705-peptide complexes with the immunodominant viral peptides: EBV EBNA3C 258-266 (RRIYDLIEL), influenza (flu) nucleoprotein NP383-391 (SRYWAIRTR), and HIV gag 264-273 (KRWIILGLNK). (ox.ac.uk)
  • Addition of exogenous H-Y peptides to male B27 cim(b/b) targets increased susceptibility to lysis to the level of cim(a/a) targets. (elsevierpure.com)
  • Replication of Associations of Genetic Loci Outside the HLA Region With Susceptibility to Anti-Cyclic Citrullinated Peptide-Negative Rheumatoid Arthritis. (ox.ac.uk)
  • Male B27 cim(b/b) cells were less efficient than cim(a/a) cells in competitively inhibiting CTL lysis of female B27 cim(a/a) targets sensitized with exogenous H-Y peptides. (elsevierpure.com)
  • A cell line (WT50) that coexpresses HLA-B7 and HLA-DR11.1 was killed by CTL-AV in the absence of any added HLA-B7 peptide. (silverchair.com)
  • The oldest theory is that of molecular mimicry, in which an autoimmune response initially is mounted against a peptide from an infectious agent and is subsequently directed against HLA-B27 itself due to epitopic similarities. (medscape.com)
  • OBJECTIVE: Genetic polymorphisms within the HLA region explain only a modest proportion of anti-cyclic citrullinated peptide (anti-CCP)-negative rheumatoid arthritis (RA) heritability. (ox.ac.uk)
  • This study was undertaken to replicate the associations of anti-CCP-negative RA with non-HLA genetic polymorphisms demonstrated in a previous study. (ox.ac.uk)
  • RESULTS: After genotyping and imputation quality control procedures, data were available for 15 non-HLA single-nucleotide polymorphisms in 1,024 cases and 6,348 controls. (ox.ac.uk)
  • Single-Nucleotide Polymorphisms Within Non-HLA Regions Are Associated With Engraftment Effectiveness for Patients With Unrelated Cord Blood Transplantation. (cdc.gov)
  • The Impact of HLA-G and HLA-E Polymorphisms on CMV Reinfection in Liver Transplant Recipients. (cdc.gov)
  • HLA-F is expressed on the cell surface of activated lymphocytes, HeLa cells, EBV-transformed lymphoblastoid cells, and in some activated monocyte cell lines. (wikipedia.org)
  • In the first trimester, HLA-F is weakly expressed in the trophoblastic elements residing outside the villi (extravillous trophoblast cells). (wikipedia.org)
  • HLA-B27 is a blood test to look for a protein that is found on the surface of white blood cells. (medlineplus.gov)
  • CD8(+) T-cells were cytotoxic against target-cells transfected with HLA-E only in the presence of specific peptide. (ucytech.com)
  • In addition, peptide induced CD8(+) T-cells also displayed regulatory activity, since they inhibited T-cell proliferation. (ucytech.com)
  • Peripheral blood mononuclear cells isolated from an individual who physiologically expressed HLA-C*04:09N triggered peptide-specific T-cell activation, confirming our results with cells with natural HLA expression levels. (haematologica.org)
  • All trademarks are owned by JPT Peptide Technologies GmbH and registered in Germany. (jpt.com)
  • Additionally, some of these polyalanine peptides are recognized by T cell lines generated against NP91-99. (ox.ac.uk)
  • Ex vivo examination of cytotoxic T cell responses to the entire HIV-1 proteome from infected subjects indicates that tapasin-dependent allotypes present a more limited set of distinct peptides than do tapasin-independent allotypes, data supported by computational predictions. (ox.ac.uk)