Peptide streamline your chromatography and mass spectrometry workflows. Medical search. Frequent questions

With new capabilities for multiplexed quantitative proteomics and native protein characterization, the Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer delivers ultimate experimental throughput, versatility, and ease-of-use. (thermofisher.com)
And as the sensitivity of mass spectrometers has improved, researchers have realized that they can finally achieve single-cell proteomics. (acs.org)
A next-generation, high-resolution mass spectrometer is designed to give proteomics, metabolomics, biopharmaceutical characterization, and small-molecule scientists the analytical performance required for research and high-throughput analyses. (selectscience.net)
Scientists are seeking technologies that can streamline and speed proteomics studies to clinical applications, reduce time to market for candidate biotherapeutics and address the growing challenge of characterizing novel chemical entities in small-molecule studies," said Iain Mylchreest, vice president, research and development, analytical instruments, Thermo Fisher Scientific. (selectscience.net)
Mass spectrometry (MS)-based single-cell proteomics (SCP) is emerging thanks to several recent technological advances in sample preparation, liquid chromatography (LC) and MS (see Kelly (2020) for a comprehensive review). (github.io)
The general workflow for processing SCP data is very similar to the workflow for bulk proteomics that we presented in the previous vignette. (github.io)
The next-generation Orbitrap Ascend Tribrid Mass Spectrometer offers advanced capabilities for multiplexed proteomics and native protein characterization. (labx.com)
The platform was recently leveraged alongside FAIMS (high-field asymmetric ion mobility spectrometry) for label-free single-cell proteomics investigations of mammalian cells with unprecedented sensitivity and proteome coverage. (labx.com)

Both liquid handlers and microfluidics coupled with analytical equipment such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have been showcased as effective for achieving this goal. (technologynetworks.com)
Agilent's InfinityLab LC/MSD iQ System for chemists and chromatographers in the company's liquid chromatography-mass spectrometry (LC/MS) portfolio features incorporated instrument health monitoring and is designed to sit beneath a stack of Agilent's InfinityLab HPLC instruments, saving lab space (1). (biopharminternational.com)
With the combination of the ReagentDrop™ dispenser, Heater-Shaker, Gripper, and Nitrogen Dryer, VERSA automated solid phase extraction (SPE) systems act to produce contamination-free sample pre-processing and trace analyte enrichment before performing sample analysis by a chromatography-based platform (HPLC, GCMS, HPLC-MS, etc). (aurorabiomed.com)

Differential mobility spectrometry (DMS) and ion mobility spectrometry are analytical techniques used to separate ions based on their gas phase mobility. (sciex.com)

Improved detection, versatility, and sensitivity for protein complex subunit analysis and biopharmaceutical characterization with an extended optional mass range up to m/z 16,000. (thermofisher.com)
A recent review by the FDA states that the use of mass spectrometry (MS) technologies detailing the characterization of biotherapeutic proteins in regulatory submissions has continued to grow and diversify over the last decade (1). (chromatographyonline.com)
The improvements in both capabilities and robustness of liquid chromatography-mass spectrometry (LC-MS) technology for biopharmaceutical analysis has led to its expanding role beyond product characterization in early development, to the monitoring of product and process attributes in late development, manufacturing, and quality control (QC) (2). (chromatographyonline.com)
We develop and use liquid chromatography-mass spectrometry-based (LC-MS) methods for in-depth characterization and quantification of complex lipids ( lipidome ), polar metabolites ( metabolome ), various exogenous compounds such as food components and drugs ( exposome ), and proteins ( proteome ). (cas.cz)

The general sample preparation strategies for different analyte classes are explored, including special considerations for sample types (fresh frozen or formalin-fixed,) strategies for various analytes (lipids, metabolites, proteins, peptides, and glycans) and how multimodal imaging strategies can leverage the strengths of each approach is mentioned. (bvsalud.org)
Molecular visualization of metabolites, lipids, and proteins by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is becoming an in-demand analytical approach to aid the histopathological analysis of breast cancer. (bvsalud.org)
Avantor has the resources to make your Chromatography or Mass Spectrometry applications run efficiently and effectively-from the measuring apparatus needed for chromatography, or the proteins used to fulfill sample manipulation during mass spectrometry. (vwr.com)
Spectrometry to Identify the Suite of Proteins That Control Metabolic. (axis-shield-density-gradient-media.com)
Over the years, the sensitivity and accuracy of mass spectrometry have advanced to the point where proteins can be reliably detected, and various other proteomic techniques have been developed and optimized, ranging from microarrays to antibody-, gel- and chromatography-based methods. (technologynetworks.com)

While new technologies enable the engineering of high titer cell clones, there is still a need to screen these clones quickly.1 The current paradigm for titer analysis depends on chromatographic approaches which, even with the newest ultra-high-performance liquid chromatography (UHPLC) models, will take minutes to analyze one cell line. (technologynetworks.com)
Scientists at the University of Sheffield looked to overcome this hurdle by developing an automated small-scale analytical platform workflow, combining purification and aggregation analysis of protein biopharmaceuticals expressed in 96 deep well plate cell cultures.4 Their workflow utilized a liquid handler equipped with a protein-A PhyTip column for purification and a size-exclusion chromatography (SEC) column for aggregate monitoring. (technologynetworks.com)
The nanoElute ® 2 is a high-performance nanoflow liquid chromatography system with an intuitive and flexible user interface, covering a broad range of applications from single cell to high throughput routine proteomics. (bruker.com)
Liquid chromatography mass spectrometry (LC-MS) is commonly used in analytical chemistry workflows to separate, identify, and quantify complex samples. (integra-biosciences.com)
Coupling liquid chromatography to mass spectrometry (LC-MS) is the preferred technique in metabolomics and lipidomics, permitting effective compound separations and detection. (cas.cz)

In September 2019, Gyros Protein Technologies AB, a provider of automated nanoliter-scale immunoassays, peptide synthesizers, and reagents, introduced Gyrolab p24 Kit for lentivirus titer determination in lentivirus vector manufacturing (2). (biopharminternational.com)
EpiCypher is a leading provider of user-friendly CUT&Tag reagents and workflows. (biolynx.ca)

With source architecture shared across all next-generation mass spectrometers, accessing the improvements from the Thermo Scientific Orbitrap Tribrid platform enables increased sensitivity, robustness, minimal optimization and maintenance, and the ability to rapidly switch between sources. (thermofisher.com)
The Thermo Scientific Orbitrap Exploris 240 mass spectrometer expands the proven Orbitrap Exploris platform and delivers mass accuracy, sensitivity and resolving power across a wide dynamic range. (selectscience.net)
Mass spectrometry (MS-) based attribute monitoring assays have been proposed as key tools to provide the sensitivity, throughput, selectivity, and flexibility required for monitoring critical product and process attributes for biopharmaceutical production and release. (chromatographyonline.com)
Boost the performance of your mass spectrometer and improve sensitivity, productivity, and data precision. (sciex.com)

Robust ion trapping for MS scans and higher energy collisional dissociation (HCD) MS/MS within the Ion-routing multipole (IRM) combines with reliable Automatic Gain Control (AGC) measurements for controlled injection of the number of ions into the fast-scanning, high-field Orbitrap mass analyzer for premium workhorse quantitative and qualitative performance. (thermofisher.com)
Robust chromatography Overlayed extracted ion chromatograms (27 peptides) of 10 consecutive injections. (bruker.com)
The InfinityLab LC/MSD iQ System is the simplest and quickest path to robust, reliable, and routine mass-based detection. (biopharminternational.com)
A fundamental step towards reliable and repeatable gas chromatography (GC) and creating a robust GC method is to achieve consistent gas flow over time and across diverse laboratories. (agilent.com)

Additionally, many of the thermal analysis methods covered below can be coupled with other analytical techniques such as gas chromatography, mass spectrometry, or microscopy in order to provide more accurate results. (excedr.com)

In this presentation, we will discuss the development of in situ/vivo approaches by cellular and subcellular mass spectrometry that enabled our lab to determine the proteomic and metabolomic profile of identified cells in live Xenopus laevis frog embryos developing to tadpoles and neurons in mouse brain tissues. (njacs.org)
Over the last decade, mass spectrometry-based metabolomics and lipidomics have become the key platforms for the comprehensive profiling of polar metabolites and complex lipids in biological samples (plasma, serum, urine, and tissues). (cas.cz)
This review communicates the recent advances in the field, with a specific focus on matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. (bvsalud.org)

The limitation has been a lack of sufficiently sensitive mass spectrometry technologies that can measure these biomolecules with compatibility for live development, a prerequisite for functional biology. (njacs.org)

Many do not have expensive mass spectrometers in their labs or mass spec teams on staff to handle these specialized analyses. (biopharminternational.com)

Improved phosphopeptide analysis Ti-IMAC beads were added to tryptic peptides for enrichment. (thermofisher.com)

Metabolites of different nominal mass, metabolites with the same nominal mass but of different molecular formula and monoisotopic mass, and metabolites of the same nominal and monoisotopic masses but with different chemical structures should be discriminated. (creative-proteomics.com)
What's more, since single metabolites can be detected in a mass spectrometer as various derived species, it is important to assign the various derived species to the parent metabolite correctly. (creative-proteomics.com)

Two analytical workflows, subunit multi-attribute monitoring (MAM) and peptide MAM, have emerged to dominate this discussion, and this article is intended to reflect on the active debates over the needs, challenges, and practical limitations for adopting MS-based attribute monitoring for late-development and QC. (chromatographyonline.com)
The Orbitrap Exploris MX Mass Detector is a part of a multi-attribute method (MAM) workflow that is designed for efficient, scalable production, to enable drug safety and to accelerate drug approval so that new drugs become available to patients faster. (biospace.com)

The links between related features across different assays are also stored to facilitate manipulation and visualization of of PSM, peptide and protein data as we go along with the processing workflow. (github.io)

Multiple fragmentation techniques - CID and HCD with optional UVPD, ETD, PCTR - are available at any stage of MS, with subsequent mass analysis in either the ion trap or ultra-high resolution Orbitrap mass analyzer. (thermofisher.com)
Both maintenance- and set up-free, EASY-IC technique ensures complete confidence with sub-ppm mass accuracy day after day, allowing smaller extraction windows or tolerances to be used for your quantitative and qualitative data analysis, streamlining data decisions - especially when combined with the exceptional resolving power of the Orbitrap Exploris 240 MS. (thermofisher.com)
Cygnus stated in a press release that its proprietary antibody affinity extraction (AAE) sample preparation method can be combined with mass spectrometry (MS) for this type of analysis. (biopharminternational.com)
Experience reproducible results while having your hands free by using our easy sample preparation protocols for peptides, including high volume dilutions for LC-MS analysis ( Figure 1 ). (integra-biosciences.com)
The ex press ion ® Compact Mass Spectrometer (CMS), the standard for mass analysis in chemistry labs worldwide, is available with a teaching package offering professors the ability to provide students with industry-related knowledge and experience in mass spectrometry. (advion.com)
The mass spectrometer was recognized for its ability to offer high resolution accurate mass (HRAM) data for intact analysis of monoclonal antibodies, oligonucleotide mass determination and peptide mapping in drug discovery applications. (biospace.com)
Our research involves applying mass spectrometry-based techniques to perform metabolomics, lipidomic and proteomic analysis of various biological samples. (cas.cz)
Metaproteomics workflow including generation of peptides and high-resolution MS analysis. (axis-shield-density-gradient-media.com)
Conducted by thermogravimetric analyzers, thermogravimetric analysis (TG) continuously measures the mass of a sample's temperature changes over time. (excedr.com)
The result of such an analysis is a plot of mass as a function of either time or temperature. (excedr.com)

The NJ Mass Spectrometry Discussion Group is pleased to announce the return of our Fall 2023 Annual Vendor Show and Symposium, after our pandemic hiatus. (njacs.org)

It is the first automated discrete analyzer designed specifically for enzyme assay applications, streamlining workflows for industrial enzyme manufacturers and enzyme users in the food, beverage, biopharmaceutical, fuel ethanol and chemical, and solid waste management industries. (biospace.com)

We used feedback from customers and service engineers on the Thermo Scientific Orbitrap Exploris 480 mass spectrometer to design a new system from the ground up to deliver the improved reliability and the exceptional performance you expect from Orbitrap mass analyzer technology. (thermofisher.com)

Stable retention times across hundreds of injections (A) Extraction of 8 peptides (400 consecutive injections). (bruker.com)
VERSA automated Solid Phase Extraction systems (also known as automated SPE systems) fully automate SPE workflows from cartridge conditioning to sample derivatization. (aurorabiomed.com)

Meet the Orbitrap Exploris 240 mass spectrometer: leading performance, application versatility and intelligent data acquisition enable exceptional data quality that is synonymous with Thermo Scientific Orbitrap technology. (thermofisher.com)
Delivering actionable insights faster than ever before by combining proven benefits of Thermo Scientific Orbitrap technology, such as sub 1-ppm mass accuracy and high resolution, with intelligent data acquisition and processing. (thermofisher.com)
The system offers positive/negative mode switching for comprehensive sample coverage, fast scan speeds and functionalities including Thermo Scientific AcquireX intelligent data acquisition workflow that enable greater automation. (selectscience.net)

Capillary electrophoresis mass spectrometry expands the molecular toolbox of cell biology and neuroscience. (njacs.org)

Reliable performance for high peptide amount. (bruker.com)

The software automatically optimizes the ion source and data acquisition parameters to ensure clean, easy-to-read peaks without being an expert in mass spectrometry. (advion.com)

Mixing 10 times at a gentle speed (6) ensures a homogeneous solution of peptides. (integra-biosciences.com)

As part of our expanded portfolio of new-generation instruments, the Orbitrap Exploris 240 mass spectrometer delivers the high performance and versatility needed to drive productivity across multiple applications. (selectscience.net)

Furthermore, many TA instruments now offer automation features which help streamline workflows and allow for researchers to focus on other priorities while analyses are performed. (excedr.com)

The review concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research, and some examples from plant biology and microbe metabolism are mentioned, to illustrate the breadth and depth of MALDI IMS. (bvsalud.org)

Built on the guiding principles of operational simplicity and reliability, the Orbitrap Exploris 240 MS combines more than 20 years of know-how with high-resolution capability and exceptional and consistent mass accuracy, empowering all users to fast-track their science. (thermofisher.com)
The new system promises to help drive discovery and identification, with increased accuracy for confident scale-up, while operational simplicity and speed streamline time to results. (selectscience.net)
Efficiency - the NovaSeq X Plus incorporates cost-efficiency throughout the workflow which includes streamlined informatics, operation simplicity, sustainability advancements, and other features that bring the cost of ownership down. (labx.com)

Deliver on your objectives with the Thermo Scientific Orbitrap Exploris 240 mass spectrometer by combining application flexibility with the performance to drive discovery and identification, plus receive the quantitative precision and accuracy you need to confidently scale up and achieve impact. (thermofisher.com)
Dr. Timothy Garrett, associate professor, department of pathology, immunology and laboratory medicine, co-director of the Southeast Center for Integrated Metabolomics, University of Florida, said, "Using the Orbitrap Exploris 240 mass spectrometer has been extremely user friendly, with one-click calibration and method templates for fast instrument setup. (selectscience.net)
The Thermo Scientific Orbitrap Exploris MX Mass Detector was also a recipient of an R&D 100 Award in the Analytical/Test category . (biospace.com)

This next generation system provides you with the highest chroma-tographic resolution resulting in exceptional peptide identification and quantification. (bruker.com)

Definitive (level 1) identification requires comparing the two or more orthogonal properties such as retention time, m/z, and fragmentation mass spectrum for the metabolite of interest to the same properties of an authentic chemical standard observed under identical analytical conditions. (creative-proteomics.com)

Our customers in the cell and gene therapy market value the workflow efficiency enabled by Gyrolab solutions, and we look forward to further supporting their efforts to develop the breakthrough therapies they are producing," said Dan Calvo, CEO and president, Gyros Protein Technologies, in a press release. (biopharminternational.com)

Technological innovation allows manufacturers to tackle these issues head on and helps to streamline and enhance production of the desired product. (technologynetworks.com)
Next-generation centrifuges from ThermoFisher are designed to tackle increasingly complex workflows using advanced components and improved performance. (labx.com)

Molecular measurements using capillary electrophoresis time-of-flight or orbitrap mass spectrometer platforms revealed proteomic and metabolomic differences between the X. laevis cells that correlated with phenotype. (njacs.org)

The system reproducibility in mass accuracy and peak integrated area over several sample batches and several days of acquisition has been impressive. (selectscience.net)
When class is not in session, the system is ideal for use for research projects, making this a versatile choice for university labs seeking state of the art equipment paired with streamlined teaching modules. (advion.com)
Read more about the Orbitrap Ascend Tribrid Mass Spectrometry system . (labx.com)

The interface simplifies the mass spectrometry experience, allowing users to quickly select the workflow and type of compound they wish to analyze in just a few clicks of the mouse. (advion.com)

The Micro-ESI 9030 detects components by optimizing the ESI probe position in relation to the sample injection port for low flow rates, maintaining microflow chromatography throughout the ion introduction process to the mass spectrometer. (biopharminternational.com)
Gas flow meter measurements generally fall into two categories: volumetric and mass, both of which measure the amount of gas exiting a column or split vent in a specific time period. (agilent.com)
The ADM Flow Meter provides two different types of gas flow meter measurement: volumetric flow (mL/min) and mass flow reported in standard cubic centimeters per minute (sccm). (agilent.com)
Mass flow values are calculated from the volumetric measurements by correcting for ambient temperature and atmospheric pressure. (agilent.com)
When in the volumetric flow mode or mass flow mode, up to four flow values can be stored on the screen for reference later. (agilent.com)

Metaproteomics: Harnessing the Power of High Performance Mass. (axis-shield-density-gradient-media.com)

These challenges are tackled with technological innovation meant to streamline and optimize the production pipeline. (technologynetworks.com)

No technology can do that yet, but mass spectrometry comes closest. (acs.org)
Imaging mass spectrometry is a well-established technology that can easily and succinctly communicate the spatial localization of molecules within samples. (bvsalud.org)

While direct measurements with inductively coupled plasma-mass spectrometry (ICP-MS) are highly efficient and feasible, only total Gd can be detected with this approach. (univie.ac.at)
Direct measurements of Gd can be obtained using anion-exchange chromatography coupled to ICP-MS but the limit of quantification will be higher. (univie.ac.at)

Chromatography labs can now eliminate complexity, increase productivity, and gain certainty with Agilent's new LC/MS which delivers an additional level of detail, ease-of-use, in a space-saving design, and with intelligence built-in," said Monty Benefiel, vice-president, and general manager of Agilent's Mass Spectrometry Division, in a press release. (biopharminternational.com)

Dr. Aron Gyorgypal is a postdoctoral research fellow at Harvard Medical School and Massachusetts General Hospital in Boston, Massachusetts. (technologynetworks.com)
Following the completion of his PhD, he joined the Anthony Lab at Harvard Medical School and Massachusetts General Hospital in the immunology department, where he leverages his engineering skillset to deepen the understanding of immunological disorders and develop therapeutic solutions against such conditions. (technologynetworks.com)

Anatomy8

Organisms7

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment74

Phenomena and Processes51

Disciplines and Occupations15

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science30

Health Care9

Geographicals1

Proteomics8

HPLC3

Mobility spectrometry1

Characterization4

Proteins5

Liquid5

Reagents2

Sensitivity4

Robust4

Analytical techniques1

Tissues3

Biomolecules1

Spectrometers1

Tryptic1

Metabolites2

Multi-attribute2

Assays1

Analysis10

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Biopharmaceutical1

Analyzer1

Extraction2

Intelligent data acquisition3

Expands1

Reliable1

Data1

Ensures1

Spectrometer delivers1

Automation1

Applications1

Simplicity3

Orbitrap Exploris3

Exceptional1

Retention time1

Cell1

Tackle2

Molecular1

System3

Analyze1

Flow5

High1

Technological1

Technology2

Measurements2

Productivity1

General2