Peptide or protein identification. Medical search. Frequent questions

The main aim of this PhD project was the identification of interesting potential HDPs, to develop novel bioactive peptides. (unina.it)
Pixian broad bean paste (PBBP) is an indispensable food widely used in many East Asian countries, yet the knowledge about bioactive peptides released from parent proteins by enzymatic hydrolysis is limited. (bioinfor.com)
The comprehensive method can be used for the investigation of bioactive peptides in complex food matrices. (bioinfor.com)
Based on peptide ranking score, a total of 20, 3 and 43 peptides were potentially regarded as bioactive peptides in hydrolysates A 9 , B 9 and P 9 , respectively. (uaeu.ac.ae)
Binding studies based on pepsite-2 modelling suggested that among 20 potential bioactive peptides in A 9 hydrolysate, only 1 (AEWLHDWKL) showed high binding towards three catalytic sites of ACE. (uaeu.ac.ae)
While, among 43 bioactive peptides of P 9 , 14 peptides were found to be potent binder to ACE catalytic sites. (uaeu.ac.ae)

By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. (nature.com)
notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. (nature.com)
The issue of abundant (often housekeeping) proteins masking regulatory proteins of lower abundance (such as signaling proteins and cytokines) continues to be a challenging issue for proteomics particularly in the case of biofluids. (hindawi.com)
While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
Proteins were validated with targeted proteomics using parallel reaction monitoring (PRM) in a validation set consisting of samples from the above patients. (frontiersin.org)
This report represents the first description of combined label-free quantitative proteomics and PRM analysis of targeted proteins for discovery of different proteins before and after IVR treatment in the same patient. (frontiersin.org)
This proposal presents an innovative approach for screening and selecting a new class of highly stable protein capture reagents and developing a new versatile approach for ligand immobilization that together enable rapid production of cyclotide-based microarrays for proteomics research. (nih.gov)
The Nature Communications paper describes CCS values measured on the timsTOF Pro as an essentially intrinsic property of the peptide ions, which can be used to improve confidence in peptide and protein group identification in 4D shotgun proteomics. (news-medical.net)
Since mass spectrometry-based proteomics relies on accurate matching of acquired spectra against a database of protein sequences, accurate CCS values offer the benefit of narrowing down the list of candidates. (news-medical.net)
This is essential for high sensitivity proteomics where low levels of peptide signals need to be accurately measured in a complex mixture, e.g. in plasma proteomics, peptidomics, immunopeptidomics or metaproteomics. (news-medical.net)
Lead author Dr. Florian Meier, now an Assistant Professor in Functional Proteomics at the Jena University Hospital in Germany, said: "The scale and precision of peptide CCS values in our data from the timsTOF Pro was sufficient to train our deep learning model to accurately predict CCS values based only on the peptide sequence. (news-medical.net)
Additionally, predicted CCS values should allow for the use of community libraries, such as the Pan Human library, a repository of over 10,000 human proteins, for targeted proteomics. (news-medical.net)
Likewise, advances in mass spectrometry proteomics now permit highly sensitive and accurate studies of protein expression at the whole proteome-scale. (biorxiv.org)

Overall, the current report remains among very few reports on identification and molecular docking of ACE inhibitory peptides from camel milk. (uaeu.ac.ae)

We have developed a new method for the identification of signal peptides and their cleavage sites based on neural networks trained on separate sets of prokaryotic and eukaryotic sequence. (nih.gov)
A synthetic peptide containing the HAV-specific amino acid sequence of one of these sites induced anti-HAV-neutralizing antibodies. (scienceopen.com)
Extracts were analyzed by LC-MS/MS and peptides identified by matching strategies, by de novo sequencing and by applying a sequence tag tool requiring similarity to the proposed sequence, but not an exact match. (ulster.ac.uk)
The only requirement for this method is that the protein or peptide must have a defined peptide sequence at its C-terminus, which is easily achieved by recombinant expression or by solid-phase synthesis. (chemistryviews.org)
Mass spectra reflect the differences in protein sequence and can therefore be reproducibly linked to a particular protein or fragment. (europa.eu)
Identifying masses to peptides of known sequence is required. (europa.eu)
This connection between the amino acids contained within a peptide sequence and its measured CCS has tremendous potential to increase the confidence of protein identification. (news-medical.net)
Professor Fabian Theis stated: "Deep learning, in particular the used recurrent neural networks need a lot of samples to be predictive, so I was very happy when Matthias approached me and we jointly were able to predict and interpolate biochemical properties of peptides based only on their sequence. (news-medical.net)
However, recent studies in many organisms and in humans have revealed significant protein sequence variation due to the presence of somatically acquired genetic variants, alternative transcription, and mRNA splicing, which are not necessarily annotated in reference databases. (biorxiv.org)
Manual and automated mass spectrometry acquisition of intact protein and peptide mass determination and determination of peptide sequence information. (lu.se)

Hence, the in silico-derived panel of potential HDPs is a rich source of peptides with pharmacologically relevant properties. (unina.it)
These studies demonstrate that camel milk is a rich source of peptides, some of which may serve as nutraceuticals. (ulster.ac.uk)

This is the first report that establishes the presence of ALPs in the neuroendocrine tissues of insects and further studies are necessary to reveal common functions of these peptides, e.g. as antimicrobial agents, ion channel modulators or classical neuropeptides. (uni-koeln.de)

A third class of CPPs are the hydrophobic peptides, containing only apolar residues with low net charge or hydrophobic amino acid groups that are crucial for cellular uptake. (wikipedia.org)
The observed agonist-bound state contains the ERRgamma LBD, a ligand (GSK4716), and the RIP140 peptide and reveals an unexpected rearrangement of the phenol-binding residues. (rcsb.org)
SP-4 (residues 192-203), SP-8 (residues 483-494), and SP-10 (residues 668-679) significantly blocked the interaction between S protein and ACE2 by biotinylated enzyme-linked immunosorbent assay, with IC 50 values of 4.30 ± 2.18, 6.99 ± 0.71, and 1.88 ± 0.52 nmol, respectively. (scienceopen.com)
Peptide scanning suggested the region spanning residues 660-683 might act as a receptor-binding domain. (scienceopen.com)
It specifically cleaves before lysine and arginine residues in proteins. (sigmaaldrich.com)
This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra. (sigmaaldrich.com)
Conventional methods involve the reaction of polymers which have one reactive end with the reactive residues of proteins/peptides. (chemistryviews.org)

Discrimination between cleaved signal peptides and uncleaved N-terminal signal-anchor sequences is also possible, though with lower precision. (nih.gov)
Cell-penetrating peptides are of different sizes, amino acid sequences, and charges, but all CPPs have the ability to translocate the plasma membrane and facilitate the delivery of various molecular cargoes to the cytoplasm or an organelle. (wikipedia.org)
Peptide libraries are established after the identification of peptide sequences. (bvsalud.org)
The identification of sequences involved in binding to erythrocytes is an important step for understanding the molecular basis of merozoite-erythrocyte interactions that take place during invasion of the Plasmodium falciparum malaria parasite into host cells. (urosario.edu.co)
Comparative surface feature analyses of the VP1 sequences of hepatitis A virus (HAV) and poliovirus type 1 allowed an alignment of the two sequences and an identification of probable HAV neutralization antigenic sites. (scienceopen.com)
Since the peptide CCS values are entirely determined by their linear amino acid sequences, they should be predictable with high accuracy and our deep learning model accurately predicted CCS values even for previously unobserved peptides. (news-medical.net)
However, most proteomic studies rely on consensus databases to match spectra to peptide and proteins sequences, and thus remain limited to the analysis of canonical protein sequences. (biorxiv.org)
11 And human diseases, including cancer in particular, tend to be defined by the presence of proteins with altered and pathogenic sequences. (biorxiv.org)
For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of anti·His antibodies. Detection of 6xHis-tagged proteins with Anti·His Antibodies (Tetra·His Antibody in the center). A: DHFR; B: DHFR; C: thioredoxin; D: TNF-α; E: TFIIA γ ; F: chaperonin; G DNA polymerase; H: TFIIAαß; for tag location and sequences detected see table 'Proteins detected with QIA express Anti·His Antibodies'. Indicated amounts of pure 6xHis-tagged protein were applied to a nitrocellulose membrane, and detection was carried out with the Anti·His primary antibody indicated diluted 1/2000, followed by chromogenic detection with AP-conjugated rabbit anti-mouse IgG and NBT/BCIP. "> "Sensitivity of QIA express Anti·His Antibodies" ). (qiagen.com)
Basic data handling of mass spectrometry data for protein identification, assessment of PTMs and determination of amino acid sequences. (lu.se)

In this study, chickpea protein hydrolysates (CPHs) obtained through Neutrase hydrolysis for 60 min (CPHs-Pro-60) exhibited the highest DPP-IVi activity. (bvsalud.org)
A total of 5867 low-molecular weight peptides were identified in the highly bioactive subfractions of the PBBP alcalase hydrolysates using traditional and peptidomics approaches. (bioinfor.com)
Camel milk protein hydrolysates (CMPHs) were investigated for anti-hypertensive and anti-inflammatory activities using in-vitro assays. (uaeu.ac.ae)

Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)

Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake and uptake of molecules ranging from nanosize particles to small chemical compounds to large fragments of DNA. (wikipedia.org)
This mechanism involves strong interactions between cell-penetrating peptides and the phosphate groups on both sides of the lipid bilayer, the insertion of positively charged arginine side-chains that nucleate the formation of a transient pore, followed by the translocation of cell-penetrating peptides by diffusing on the pore surface. (wikipedia.org)

In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)

Milk proteins were determined by (1) two-dimensional gel electrophoresis and peptide mass mapping and (2) liquid chromatography-tandem mass spectrometry (LC-MS/MS) following one-dimensional polyacrylamide gel electrophoresis. (ulster.ac.uk)

LC-MS/MS and bioinformatics analysis were performed to identify differentially expressed proteins. (frontiersin.org)
A set of tools for detecting differentially abundant peptides and proteins in shotgun mass spectrometry-based proteomic experiments with tandem mass tag (TMT) labeling. (bioconductor.org)

Recent molecular and structural analyses of representative gluten proteins, most notably alpha- and gamma-gliadin proteins from wheat, have improved our understanding of these pathogenic mechanisms. (nih.gov)

Moreover, endosomal sorting complex required for transport (ESCRT)-related proteins and their transcripts are most abundant at early and mid-development. (nature.com)

However, for the protein follistatin it was necessary to include trypsin digestion in the sample preparation, which considerably increases the overall analysis time. (ku.dk)
LysargiNase is suitable for peptide or protein digestion in solution or in gel. (sigmaaldrich.com)
For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended. (sigmaaldrich.com)

The "cargo" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. (wikipedia.org)
The first step in this proposed model is an interaction with the unfolded fusion protein (TAT) and the membrane through electrostatic interactions, which disrupt the membrane enough to allow the fusion protein to cross the membrane. (wikipedia.org)
In the first dimension, ion-exchange chromatography (IEC) separates peptides based on ionic interactions with the solid phase and an increasing salt buffer. (hindawi.com)
co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)

Protein dynamics data are therefore sought to better describe homeostatic processes and enhance the utility of phenotyping-by-omics approaches. (nature.com)
We investigated the peptide inventory of the corpora cardiaca (CC) of the honey bee, Apis mellifera, by direct tissue profiling using MALDI-TOF MS combined with proteomic approaches focusing on cysteine-containing peptides. (uni-koeln.de)
Current approaches for peptide and protein identification use advanced statistical and graphical methods to match mass spectra and estimate their confidence. (biorxiv.org)
However, there is interest in the possibility of Peptide reactivity exploiting these same experimental approaches to distinguish between different forms of chemical aller- gens - as individual materials are primarily associated with one or the other form of sensitization in humans. (cdc.gov)
These opportunities are surveyed here against a background of the immunobiology of allergic sensitization and current state-of-the-art approaches to measurement of peptide/protein reactivity. (cdc.gov)

Finally, in silico analysis of the gluten proteome led to the identification of at least 60 putative peptides that share the common characteristics of the 33-mer and the 26-mer peptides. (nih.gov)
A putative Vibrio cholerae two-component system controls a conserved periplasmic protein in response to the antimicrobial peptide polymyxin B. (broadinstitute.org)
We used massively parallel cDNA sequencing (RNA-Seq) to quantitatively identify the transcriptome of V. cholerae grown in the presence and absence of sub-lethal concentrations of the antimicrobial peptide polymyxin B. We evaluated the transcriptome of both wild type V. cholerae and a mutant carrying a deletion of vc1639, a putative sensor kinase of an uncharacterized two-component system, under these conditions. (broadinstitute.org)

A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
This project aims to develop a revolutionary screening platform that will allow for the rapid isolation of hundreds of high affinity and specificity synthetic ligands for proteins in a highly parallel fashion. (nih.gov)

The spectra represent samples with both high and low protein abundance, and it is shown that peak sets selected by human operators have a considerable variability and that it is impossible to speak of a single true peak set. (lu.se)
Because proteostatic events often trigger zero net change in protein abundance but instead alter protein temporal dynamics 11 - 13 , they typically elude conventional experiments that measure only the steady-state abundance of proteins. (nature.com)
In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)
Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. (nature.com)

Analogous to the 33-mer, the synthetic 26-mer peptide displayed markedly enhanced T cell antigenicity compared to monovalent control peptides. (nih.gov)
Since then, the number of known CPPs has expanded considerably, and small molecule synthetic analogues with more effective protein transduction properties have been generated. (wikipedia.org)
Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. (scienceopen.com)
LC-MS, SRM, DIA(SWATH) with label-free or labeled synthetic peptides can be analyzed with other R package, MSstats. (bioconductor.org)

HDPs are seen as true multifunctional peptides with activities as diverse as chemotaxis, inhibition of LPS-induced inflammation, modulation of leukocyte differentiation and promotion of wound healing. (unina.it)
In this study, to prepare the fraction containing multifunctional cationic peptides, we first hydrolyzed rice bran protein (RBP) with pepsin. (listlabs.com)

We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
The detection of norovirus capsid protein was explored using three common MS-based methods: scanning of intact proteins, peptide mass fingerprinting, and peptide sequencing. (cdc.gov)

A completely automated peak detection strategy is developed that avoids time-consuming parameter tuning, and which yields protein identification efficiency that exceeds the human operator. (lu.se)
To accelerate research in this field, new protein capture tools for the detection and identification of specific proteins are needed. (nih.gov)
Anti·His Antibodies enable identification of positive expression clones by colony blotting (see figure Identification of positive expression clones by colony blotting. . "> "Identification of positive expression clones by colony blotting" ) and detection of His-tagged thioredoxin (see figure His-tagged thioredoxin detected by Penta·His Antibody in yeast cells. . "> "His-tagged thioredoxin detected by Penta·His Antibody in yeast cells" ). (qiagen.com)
The QIA express System provides materials for the expression, purification, detection and assay of 6xHis-tagged proteins. (qiagen.com)
Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry. (cdc.gov)
Mass spectrometry (MS) represents a rapid technique for the identification of microbial monocultures, and its adaptation to the detection of pathogens in real-world samples is a public health and homeland security priority. (cdc.gov)
Detection of intact target protein was limited by poor selectivity and sensitivity. (cdc.gov)
Detection of up to 16 target peptides by peptide mass fingerprinting allowed for the reproducible and confident (P (cdc.gov)
Factors that might affect the linearity and dynamic range for detection of BoNT cleavage products were initially examined, including the amount of peptide substrate and internal standard, the timing of cleavage reaction, and the components in the reaction solution. (cdc.gov)
Enzymatic cleavage assays represent an attractive approach where the functional active toxins are detected by monitoring the cleavage of their target substrates, or peptide mimics in most cases, by means of different techniques, such as immunological detection 18 , 19 , capillary electrophoresis 20 , fluorescence analysis 21 , Forster resonance energy transfer (ALISSA) 22 , and high performance liquid chromatography 23 . (cdc.gov)

The described method therefore allows automated user-independent protein identification using peptide mass fingerprints. (lu.se)
In addition, we purified the cationic peptides from fractions exhibiting antimicrobial activity by reversed-phase high-performance liquid chromatography and identified them by matrix-assisted laser/desorption ionizationtime-of-flight mass spectroscopy. (listlabs.com)
Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)
19 short peptides (3-5 amino acids) were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, including 5 tripeptides, 8 tetrapeptides, and 6 pentapeptides. (bioinfor.com)
An agatoxin-like peptide (ALP) was identified as a component of the glandular part of the CC and was associated with the presence of the adipokinetic hormone in mass spectra. (uni-koeln.de)
During my MSc I developed an identification system for fishbone: ZooMS (Zooarchaeology by Mass Spectrometry), based upon protein barcoding. (europa.eu)
As proteins can be cleaved enzymatically and analyzed by mass spectrometry in a repeatable way, protein barcoding is used widely for quick and inexpensive protein identification. (europa.eu)
Most commonly, this approach leverages high-resolution mass spectrometric measurements of proteolyzed proteins, combined with tandem peptide fragmentation to match observed spectra with those expected from their amino acid composition. (biorxiv.org)
With the exception of completely de novo mass spectrometry identification algorithms which currently lack the requisite accuracy and efficiency for genome-scale studies, all current methods depend on matching observed mass spectra to reference proteome databases. (biorxiv.org)
Make an experimental plan for preparing and analyzing protein and peptide samples using mass spectrometry. (lu.se)
The course gives an introduction in how to use protein mass spectrometry as a tool in protein science. (lu.se)
Practical parts including protein and peptide sample preparations, deposition with different techniques and the use of chromatographic separations for complex samples prior to mass spectrometry analysis. (lu.se)

In order to identify by a rational approach further human proteins carrying cryptic HDPs, we recently developed an in-silico screening method to localize antimicrobial regions hidden inside the primary structure of precursor proteins. (unina.it)
By means of a multidisciplinary approach including biochemical, cellular biology and spectroscopic techniques, the action mechanism of both peptides has been elucidated, and intriguing results have been obtained by testing their immunomodulatory and anti-cancer activities. (unina.it)
Here, we describe an experimental approach that fractionates proteins released from human platelets, linking bioassay activity to identity. (hindawi.com)
A previous study from our laboratory using a MuDPIT (multidimensional protein identification technology) approach identified over 300 proteins secreted by platelets upon thrombin activation [ 1 ]. (hindawi.com)
5848 medium-sized peptides (6-10 amino acids) were characterized using the peptidomics approach, including 1484 hexapeptides, 1217 heptapeptides, 1634 octapeptides, 927 nonapeptides, and 586 decapeptides. (bioinfor.com)
Ashutosh Chilkoti and colleagues, Duke University, Durham, NC, USA, developed a high-yield synthesis of site-specific protein/peptide-polymer therapeutics by using a modular approach involving recombinant expression, enzymatic attachment of an azido group to the protein/peptide, and polymer coupling by a copper-free click reaction (pictured). (chemistryviews.org)
In the present work we searched for p190-derived breakpoint peptides suitable for a peptide vaccine approach in vivo. (hindawi.com)
In a disease-crossing approach, RNA binding proteins, which were recently found not only to be deposited in Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis but also in many AD cases, will be investigated for their potential to modulate Aß aggregation and AD pathology. (alzheimer-europe.org)
A Graph-Centric Approach for Metagenome-Guided Peptide and Protein Identification in Metaproteomics. (cdc.gov)

We demonstrated that both peptides are endowed with a significant antimicrobial activity towards Gram-negative and Gram-positive strains, and are able to prevent biofilm formation in several strains at concentrations lower than those required to directly kill planktonic bacterial cells. (unina.it)

An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
gp120 peptide is used for blocking the activity of presenilin1 antibody. (prosci-inc.com)
Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. (qiagen.com)

Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)

Second, proteolytic analysis of a representative gamma-gliadin led to the identification of another multivalent 26-mer peptide that was also resistant to further gastric, pancreatic and intestinal brush border degradation, and was a good substrate of human transglutaminase 2 (TG2). (nih.gov)
Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity. (expasy.org)

Sensitivity may vary with different proteins. (qiagen.com)

Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)

Many human proteins with functions not necessarily related to host defense behave as sources of HDPs. (unina.it)

Over 200 proteins were identified: some known camel proteins including heavy-chain immunoglobulins and others exhibiting regions of exact homology with proteins from other species. (ulster.ac.uk)

We separated the enzymatic hydrolysate of RBP into 20 fractions containing peptides with different isoelectric point (pI) values by ampholyte-free isoelectric focusing (autofocusing). (listlabs.com)
Fractions containing cationic peptides obtained from RBP hydrolysate have the potential to be used as dietary supplements and functional ingredients in food products. (listlabs.com)
Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1. (hindawi.com)

VisP is a conserved periplasmic protein implicated in lipid A modification in Salmonellae. (broadinstitute.org)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)

Together, these results highlight the pivotal role of physiologically generated, proteolytically stable, TG2-reactive, multivalent peptides in the immune response to dietary gluten in Celiac Sprue patients. (nih.gov)
Store gp120 peptide at -20°C, stable for one year. (prosci-inc.com)

The source code is publicly available so that further developments can be accelerated for training and prediction models of the human peptide universe. (news-medical.net)

We present an overview of protein and peptide compounds confiscated in Denmark from late 2007 till late 2013 together with a description of a newly developed HRAM-LC-MS method used for identification. (ku.dk)
the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

performs statistical analysis steps, that follow peptide identification and quantitation. (bioconductor.org)

In this study, Pf RhopH3 consecutive peptides were synthesized and tested in erythrocyte binding assays for identifying those regions mediating binding to erythrocytes. (urosario.edu.co)
These high-activity binding peptides (HABPs) were characterized by having a-helical structural elements, as determined by circular dichroism, and having receptors of a possible sialic acid-dependent and/or glycoprotein- dependent nature, as evidenced in enzyme-treated erythrocyte binding assays and further corroborated by cross-linking assay results. (urosario.edu.co)
11 proteins were up-regulated and 17 proteins were down-regulated, while consistent presence/absence expression profile group contains one elevated protein and nine reduced proteins, among which seven proteins were identified as potential biomarkers for IVR treatment through PRM assays. (frontiersin.org)

Molecular docking verified that the four screened peptides (AAWPGHPEF, LAFP, IAIPPGIPYW, and PPGIPYW) could bind to the active center of DPP-IV. (bvsalud.org)
Further insight into molecular mechanism of binding revealed that 4 peptides were able to bind to active site of ACE with good docking scores and MM-GBSA binding energies. (uaeu.ac.ae)

Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)

Bioinformatics analysis indicated the up-regulated proteins were significantly enriched in "GnRH secretion" and "Circadian rhythm" signaling pathway. (frontiersin.org)

Regulation of proteome integrity requires chaperone-assisted folding of unfolded proteins, dissolution of misfolded aggregates, proteolytic removal of proteins, and other concerted proteostatic processes 1 , 2 . (nature.com)
The present dataset was collected using a technology platform we recently developed, which overcame several technical challenges in quantifying individual protein turnover rates on a proteome scale. (nature.com)
Prolyl endopeptidase treatment was shown to abolish the antigenicity of both the 33-mer and the 26-mer peptides, and was also predicted to have comparable effects on other proline-rich putatively immunotoxic peptides identified from other polypeptides within the gluten proteome. (nih.gov)
We acquired data from whole-proteome digests of five organisms, which resulted in the measurement of over two million CCS values, including about 500,000 unique peptides, making it by far the most comprehensive CCS data set to date. (news-medical.net)
Effects of insulin and the glucagon-like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice. (lu.se)

As onset and progression of AD is triggered by the amyloid cascade, I will put particular attention on amyloid ß-peptide (Aß). (alzheimer-europe.org)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

Indigenous peptides were also identified following isolation and concentration by two strategies: (1) gel-eluted liquid fraction entrapment electrophoresis and (2) small-scale electrophoretic separation. (ulster.ac.uk)

Thus, endomembrane-modifying proteins within the endomembrane system will have an influence on the final grain quality/yield and recombinant protein production. (nature.com)

Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)

Furthermore, these HABPs inhibited merozoite in vitro invasion of normal erythrocytes at 200 mM by up to 60% and 90%, suggesting that some RhopH3 protein regions are involved in the P. falciparum erythrocyte invasion. (urosario.edu.co)
We identified one peptide named p190-13 able to induce in vitro peptide-specific CD4+ T cell proliferation in Ph+ ALL patients in complete remission during TKIs. (hindawi.com)

This mechanism explains how key ingredients, such as the cooperation among the peptides, the large positive charge, and specifically the guanidinium groups, contribute to the uptake. (wikipedia.org)
CMPHs with potent ACE inhibitory and anti-inflammatory activities were subjected to peptide identification and their possible binding mechanism to ACE were depicted via molecular docking. (uaeu.ac.ae)
Hypercalcemia secondary to gastrointestinal stromal tumors: parathyroid hormone-related protein independent mechanism? (medscape.com)

It is known to act through protein phosphorylation via PRKA and through the activation of guanine nucleotide exchange factors like EPAC. (plos.org)

The effective synthesis and delivery of polymer conjugates of protein and peptide therapeutics are challenges in clinical development. (chemistryviews.org)
Our identification of a genetic variant of TFB1M , encoding a protein that controls protein synthesis in mitochondria, which is associated with increased risk of Type 2 Diabetes, has been a strong thrust. (lu.se)

Anatomy11

Organisms11

Diseases1

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment52

Phenomena and Processes54

Disciplines and Occupations4

Technology, Industry, Agriculture4

Information Science15

Health Care7

Bioactive peptides6

Proteomics13

Inhibitory peptides from camel1

Sequence10

Rich source of peptides2

Functions of these peptides1

Residues7

Sequences10

Hydrolysates3

Characterization1

Cell-penetrat2

Quantification1

Milk Proteins1

Differentially2

Pathogenic1

Abundant1

Digestion3

Interactions4

Approaches5

Putative3

High affinity and specificity2

Abundance4

Synthetic4

Multifunctional2

Intact proteins2

Detection10

Mass12

Approach9

Antimicrobial activity1

Antibody3

Proteomic1

Substrate2

Different proteins1

Thrombin1

Human proteins1

Homology1

Fractions3

Modification1

Molecules2

Stable2

Prediction1

Compounds2

Quantitation1

Assays3

Bind2

Bacterial1

Bioinformatics1

Proteome5

Amyloid2

Electrophoresis1

Recombinant protein1

Substrates1

Vitro2

Mechanism3

Phosphorylation1

Synthesis2