Peptide nucleic acids mimic oligonucleotides. Medical search. Frequent questions

This mini-review focuses on covalently linked conjugates of PNA with cell-penetrating peptides, aminosugars, aminoglycoside antibiotics, and non-peptidic molecules that were tested, primarily as PNA carriers, in antibacterial and antiviral applications. (bvsalud.org)
METHODS: A set of antisense P-PNA conjugates with a bacterial penetration peptide were designed to inhibit the expression of gyrA and parC and were evaluated for their antibacterial activities. (bvsalud.org)
We therefore synthesized conjugates of the peptide FF to the peptide nucleic acid dimer "gc" and characterized their aggregates by different spectroscopic techniques, including NMR, CD and fluorescence. (cnr.it)

In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software. (wikipedia.org)
Peptide nucleic acids (PNAs) are synthesized by attaching modified peptide backbones generally derived from repetitive N-2-aminoethyl glycine units in place of the regular phosphodiester backbone and represent synthetic impersonator of nucleic acids that offers an exciting research field due to their fascinating spectrum of biotechnological, diagnostic and potential therapeutic applications. (bvsalud.org)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Dynamic and flexible nucleic acid models can provide current and future scientists with physical intuition for the structure of DNA and the ways that DNA and its synthetic mimics can be used to build self-assembling structures and advanced nanomachines. (allenpress.com)
3,4 Additionally, the stability of the alkyne group during solid-phase synthesis enables the post-synthetic labelling of oligonucleotides. (biosearchtech.com)
Click ligation has been employed to generate stable 5′-5′ and 5′-3′ strand ligation, the synthesis of cyclic DNA catenanes (synthetic mimics of mini-plasmids), mini-DNA duplexes, and duplexes that are covalently cross-linked across the nucleobases. (biosearchtech.com)
In this study, we found that both naive and memory B cells lack TLR4 (receptor for LPS) but express TLR9 (receptor for CpG motifs) and produce IL-6, TNF-α, and IL-10 upon stimulation with CpG oligonucleotides (ODN), synthetic mimics of microbial DNA. (aai.org)

This technology was recently used to achieve stepwise total chemical synthesis of protein chains nearing 200 amino acids in length that retained the structure and function of native variants obtained by recombinant expression. (aps2022.org)
Further, chemical synthesis enables the ability to rewire the covalent framework with non-natural amino acids, drugs, and carbohydrates. (aps2022.org)
I will discuss the fully automated single-shot chemical synthesis of protein chains up to 220 amino acids in hours. (aps2022.org)
When a defined protein/peptide (or combinations thereof) control and define the synthesis of an inorganic nanoparticle, the result is a cloneable NanoParticle (cNP). (rsc.org)
This is particularly useful when attaching dyes to oligonucleotides that cannot be incorporated during synthesis because they may degrade under the strong basic conditions necessary for DNA and RNA cleavage and deprotection. (biosearchtech.com)

Fluorescence-labelled oligonucleotides have applications as probes and primers for molecular assays such as fluorescent in situ hybridisation (FISH), quantitative PCR, sequencing and live cell imaging, which are routinely used in molecular diagnostics and drug development. (biosearchtech.com)

the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)
Here we discuss two approaches currently used to investigate the global dynamics of phosphorylation based on the isolation of phosphorylated proteins or peptides. (acs.org)

This 'protein pump' can deliver a variety of non-native cargo molecules into cells including antibody mimics, mirror-image proteins, small molecules, enzymes, and antisense oligonucleotides. (aps2022.org)
Therapeutic tiny or short modified antisense oligonucleotides (Tiny ASOs) can block gene expression or modulate splice-switching! (biosyn.com)

Phosphoryl guanidine oligo-2′-O-methylribonucleotides in neutral therapeutic oligonucleotides enable enhanced cell penetration! (biosyn.com)
Aptamers 2016 will be co-hosted with our 2nd symposium on antisense and therapeutic oligonucleotides, Oligo 2016 , on 6th April 2016. (libpubmedia.co.uk)

Reconstitution of hmtRNAMet from U8 and 8U→C D-half-molecules with T-half-molecules required Mg2+ and was assessed by gel mobility shift assays using native 15% PAGE in Tris borate buffer (89 mm Tris base, 89 mm boric acid, pH 8.3) with and without 3 mm Mg2+ at 4 °C (25Jones C. Spencer A.C. Hsu J. Spremulli L.L. Martinis S.A. DeRider M. Agris P.F. J. Mol. (ncsu.edu)
These groups are compatible with biological molecules, as they do not react with the functional groups found in nucleic acids, proteins and lipids. (biosearchtech.com)
Molecules containing both aromatic peptides and nucleic acids could in principle exploit different forces to self-assemble. (cnr.it)

Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)

The semi-rigid peptide nucleic acid backbone serves as a nearly-perfect template for attaching complimentary base pairs on DNA or RNA in a sequence-dependent manner as described by Watson-Crick models. (bvsalud.org)
Therefore, the deprotonation and coordination of the peptide backbone occur only in a strongly alkaline solution. (cnr.it)
in case of nucleic acids, Watson-Crick pairing drives self-assembly while, in case of peptides, backbone hydrogen bonds and interactions between aromatic side chains trigger the formation of structures, such as nanotubes or ribbons. (cnr.it)

As more research labs and classrooms dive into the field of structural nucleic acid nanotechnology, students and researchers need access to interactive, dynamic, handheld models. (allenpress.com)
Nucleic Acid Nanotechnology (pp. ix-x). (au.dk)
2014). Nucleic Acid Nanotechnology . (au.dk)

The technique is widely used to label DNA, RNA and locked nucleic acid (LNA) oligonucleotides, as well as to cyclise DNA, join oligonucleotides to peptide nucleic acids (PNA), synthesise DNA catenanes and create analogues of DNA with modified nucleobases and backbones. (biosearchtech.com)

Click DNA labelling is commonly used to post-synthetically conjugate alkyne-labelled DNA to azide-functionalised reporter groups such as fluorescent dyes, sugars and peptides. (biosearchtech.com)

They also overcame the cytosolic delivery barrier with cell-penetrating peptides, chemical vectors that facilitate cellular uptake and nuclear targeting of antisense cargo. (aps2022.org)

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

The aim of the present study was to determine the secondary structures of the cementoin domain and to characterize the antibacterial properties of these peptides against P. aeruginosa . (biomedcentral.com)
The N-terminal cementoin domain adopts α-helical secondary structures in a membrane mimetic environment, which is common in antimicrobial peptides. (biomedcentral.com)
Pol II, assisted by the neurodegeneration-associated enzyme senataxin, generates a shield comprising triplex nucleic acid structures known as R-loops at intergenic spacers flanking nucleolar rRNA genes. (nature.com)
Peptides and nucleic acids can self-assemble to give supramolecular structures that find application in different fields, ranging from the delivery of drugs to the obtainment of materials endowed with optical properties. (cnr.it)

Peptide nucleic acid (PNA) is a nucleic acid mimic with high specificity and binding affinity to natural DNA or RNA, as well as resistance to enzymatic degradation. (bvsalud.org)

The antimicrobial wasp Polybia-MP1 peptide selectively kills bacterial cells! (biosyn.com)
We show here that the cementoin domain adopts an α-helical conformation both by circular dichroism and nuclear magnetic resonance analyses in the presence of membrane mimetics, a characteristic shared with a large number of linear polycationic antimicrobial peptides. (biomedcentral.com)
However, unlike numerous linear polycationic antimicrobial peptides, membrane disruption does not appear to be the main function of either cementoin, elafin or full-length pre-elafin/trappin-2 against P. aeruginosa . (biomedcentral.com)
In the past decade, gene-encoded short positively charged peptides, collectively known as antimicrobial peptides (AMP), have attracted much attention because of their broad antimicrobial activities and their potential use as therapeutics [ 11 - 18 ]. (biomedcentral.com)

The American Peptide Society, APS, a nonprofit scientific and educational organization founded in 1990, provides a forum for advancing and promoting knowledge of the chemistry and biology of peptides. (aps2022.org)
Here we will briefly examine how click chemistry can be used to modify biomolecules used in molecular biology techniques, particularly oligonucleotides, as well as proteins and antibodies. (biosearchtech.com)
Click chemistry can efficiently immobilise oligonucleotides onto hard or soft substrates, such as glass, silicon and polymers. (biosearchtech.com)
Several studies have demonstrated the use of click chemistry in a variety of nucleic acid assemblies, including ligation, circular constructs and catenanes. (biosearchtech.com)

In several examples, the α4 integrin antagonist is a monoclonal antibody that specifically binds to a α4, β1 or β7 integrin subunit or a cyclic hexapeptide with the amino acid sequence of CWLDVC. (justia.com)

The order of amino acids as they occur in a polypeptide chain. (lookformedical.com)

The results indicated that the macrochelate coordinated zinc(ii) complexes are dominant in a whole pH-range and the side chain donors of the peptides are involved in the metal binding. (cnr.it)
The acetylation of the peptide amino terminus (L2) significantly enhances the zinc(ii) binding ability compared to the corresponding nickel(ii) complexes. (cnr.it)

Development of 7SK snRNA mimics that inhibit HIV transcription. (kit.ac.jp)

Building on previous research, representative nucleic acid duplexes were split into logical monomer segments, and atomic coordinates were used to create solid models for 3D printing. (allenpress.com)

Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
Fusion proteins, peptides, and peptibodies (proteins fused to antibodies) form a separate group of therapeutics. (biopharmatrend.com)

Provided herein are anti-angiogenic comprising the N-terminal end of endostatin, nucleic acids encoding the same, pharmaceutical preparations comprising an effective amount of the peptide and nucleic acids and use of the pharmaceuticals in treating or preventing diseases or conditions associated with undesirable angiogenesis. (justia.com)

Pepsin is a protease and acts to digest proteins into peptides. (wikipedia.org)
The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)

Leveraging expertise with high-throughput identification of peptides from ultra-large combinatorial libraries, the Pentelute group has developed an affinity selection-mass spectrometry platform for rapid de novo discovery of potent binders to proteins in solution. (aps2022.org)

A common feature ascribed to AMP is their ability to interact with the negatively charged bacterial membranes and polyanionic cell surface (lipopolysaccharide (LPS) of Gram-negative and lipoteichoic acid of Gram-positive bacteria). (biomedcentral.com)

Functional recognition of the modified human tRNA(UUU)(Lys3) anticodon domain by HIV's nucleocapsid protein and a peptide mimic. (ncsu.edu)
Typically, azide-functionalised DNA, PNA or modified nucleic acids are linked to alkyne end-functional diblock copolymers. (biosearchtech.com)

Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. (thermofisher.com)

The first Symposium on Oligonucleotide Technologies and Therapeutics in Portugal (OTP2021) wants to bring together national and international scientists from academia and industry, as well as the medical community, interested in nucleic-acids-based technologies and therapeutics. (up.pt)

Nucleic Acids Research, 38(9), 3094–3105. (ncsu.edu)
NUCLEIC ACIDS RESEARCH, 36(20), 6548–6557. (ncsu.edu)

This platform is currently applied for discovery of non-canonical peptide disruptors of cancer protein-protein interactions that are effective, non-toxic, long-circulating, and stable in cells and animals. (aps2022.org)
However, chemical and enzymatic probing has lead to the idea that these tRNAs have retained the basic cloverleaf structure of canonical tRNAs but that they lack several conserved tertiary interactions leading to a weaker three-dimensional structure (4Watanabe Y.-I. Kawai G. Yokogawa T. Hayashi N. Kumazawa Y. Ueda T. Nishikawa K. Hirao I. Miura K.-I. Watanabe K. Nucleic Acids Res. (ncsu.edu)
Didier Trono s'est beaucoup intéressé aux interactions entre les virus et leurs hôtes, ce qui l'a conduit à étudier la biologie de pathogènes comme le VIH et le virus de l''hépatite B, et à créer des outils de transfert génétique dérivés du VIH aujourd'hui utilisés avec succès en thérapie génique. (epfl.ch)
Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)

Endostatin, a 183 amino acid proteolytic cleavage fragment corresponding to the C-terminus of collagen 18, has been the subject of investigation by a number of laboratories because of its anti-tumor activity with no toxic side effects (O'Reilly et al. (justia.com)
Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)

A sophisticated high-order framework nucleic acid (FNA) was engineered for the targeted delivery and responsive release of environment tolerant antisense peptide nucleic acids (asPNAs). (bvsalud.org)

Harnessing the power of machine learning, best-in-class variants were predicted and designed de novo, outperforming all previously known peptides for antisense delivery, while being non-toxic and effective in mice. (aps2022.org)

89, No. 21: 10537, 1992), a relatively non-toxic peptide that disrupts viral fusion thereby protecting CD4+ lymphocytes from de novo infection. (justia.com)

However, small amounts of the aminoacylated 8U→C mutated hmtRNAMet could be isolated, permitting a limited investigation of additional steps in translation.In the mammalian mitochondrial system, the Met-tRNAMet must be formylated by the mitochondrial transformylase (MTFmt) to be used in initiation (19Spencer A.C. Spremulli L.L. Nucleic Acids Res. (ncsu.edu)

Solid Acid Electrochemical Cells (SAECs), which most commonly employ CsH2PO4 (CDP) as the electrolyte component, are uniquely suited to meet the. (northwestern.edu)
Recently, the group retargeted the anthrax protective antigen to receptors overexpressed on tumor cells and modified its lethal factor component to target cancer gene dependencies with antisense peptide nucleic acids. (aps2022.org)
E. coli BL21 cells, carrying the pET15-bovine MTFmt plasmid construct, were grown as described (19Spencer A.C. Spremulli L.L. Nucleic Acids Res. (ncsu.edu)

A benzoic acid derivative belonging to the meglitinide class of antidiabetic agents. (medindex.am)

Nucleic acid represents the ideal drug candidate for protein targets that are hard to target or against which drug development is not easy. (bvsalud.org)

The [35S]Met and [35S]fMet in 5 ;l of each reaction were separated on Partisil LK5D TLC plates (Whatman) with a butanol:acetic acid:water (4:1:1) mixture. (ncsu.edu)

5′-32P-GAACCGGACCGAAGCCCG, obtained from the Nucleic Acids Core Facility at University of North Carolina) was added to the 1M7-treated, DMSO-treated, or untreated RNA (2 pmol), and the samples were incubated at 65 °C for 5 min and then at 35 °C for 20 min for primer annealing. (ncsu.edu)

OBJECTIVES: To examine the bactericidal effect of antisense peptide-peptide nucleic acids (P-PNAs) that can block the expression of DNA gyrase or topoisomerase IV in FQ-resistant Escherichia coli (FRE). (bvsalud.org)

Il participe aujourd'hui activement à la coordination des efforts de la Suisse en vue de l'intégration des nouvelles technologies dans le domaine de la médecine de précision et de la santé personnalisée. (epfl.ch)

Anatomy2

Diseases2

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes14

Information Science3

Health Care1

Conjugates3

Synthetic7

Synthesis5

Fluorescence1

Proteins or peptides2

Antisense oligonucleotides2

Therapeutic2

Molecules3

Aptamers1

Backbone3

Nanotechnology3

Backbones1

Fluorescent dyes1

Cellular uptake1

Sequences1

Structures4

Specificity1

Antimicrobial4

Chemistry4

Antibody1

Polypeptide1

Complexes2

Inhibit1

Duplexes1

Antibodies2

Preparations1

Protease2

Combinatorial1

Bacterial1

Functional2

Amyloid1

Symposium1

Research2

Interactions4

Amino acid2

Framework1

Delivery1

Fusion1

Small1

Cells3

Class1

Target1

Mixture1

University1

Effect1

Technologies1