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  • target
  • In this paper, we propose a new tool for genetic engineering, the pseudocomplementary peptide nucleic acid nucleases (pcPNANs), which are composed of a pseudocomplementary PNA (pcPNA) specific for a DNA target sequence, a FokI nuclease cleavage domain and a nuclear localization signal. (peerj.com)
  • found
  • We found that conjugation of cell penetrating peptide (CPP) to PNA is essential for the antimicrobial effect, with CPP1 [(KFF)3K] being the most effective against E. amylovora . (frontiersin.org)
  • ligation
  • After ligation to the modular peptide-based BioShuttle carrier, which was utilized to facilitate the delivery of the functional modules into the cells' cytoplasm, the modules were scrutinized. (medsci.org)
  • structural
  • Polypeptide structures have the advantage of structural integrity and robustness while nucleic acids have the advantage of specific molecular recognition between complementary bases. (esrf.eu)
  • Computational techniques, and in particular molecular dynamics (MD) simulations, have been successfully used as a complementary technique to predict and analyse the structural behaviour of nucleic acids, including peptide nucleic acid- (PNA-) RNA hybrids. (hindawi.com)
  • molecular
  • Our group has been extensively involved in the study of molecular self-assembly by extremely short peptide fragments. (esrf.eu)
  • Nowadays the solid phase synthesis (SPPS) chemistry emerges as a key technology and is considered as a promising methodology to design peptides for the investigation of molecular pharmacological processes at the transcriptional level. (medsci.org)
  • They show promise for the development of gene therapeutic agents, diagnostic devices for genetic analysis, and as molecular tools for nucleic acid manipulations. (chalmers.se)
  • stable
  • We are currently looking into designing small molecules such as specific peptide nucleic acids , which are stable and can be easily delivered to block the cancer-driven RNA editing process, said Prof Tan, who is also from the Duke-NUS Medical School and Genome Institute of Singapore (GIS). (thefreedictionary.com)
  • 2017
  • In the Global Peptide Nucleic Acids Industry Market Analysis & Forecast 2018-2023, the revenue is valued at USD XX million in 2017 and is expected to reach USD XX million by the end of 2023, growing at a CAGR of XX% between 2018 and 2023. (researchbeam.com)
  • chemistry
  • They are assembled using techniques adapted from peptide chemistry. (currentprotocols.com)
  • In this context our modified solid phase peptide chemistry using temperature shifts during synthesis is considered as an appropriate technology. (medsci.org)
  • In general, an appropriate solid support must be cleavable in acid, leaving a C-terminal amide, and be compatible with Fmoc chemistry. (linktech.co.uk)
  • syntheses
  • SPPS syntheses could be carried out in core facilities producing peptides for large-scale scientific implementations as presented here. (medsci.org)
  • Methods
  • Advances in imaging diagnostics using magnetic resonance tomography (MRT), positron emission tomography (PET) and fluorescence imaging including near infrared (NIR) imaging methods are facilitated by constant improvement of the concepts of peptide synthesis. (medsci.org)
  • cationic
  • The influence of chain modification - PNA vs. DNA - and type of redox marker - anionic AQMS-Na (sodium salt of anthraquinone-2-sulfonic acid) and Fe(II/III) (potassium ferri/ferrocyanide) or cationic MB (methylene blue) and RuHex (hexaammineruthenium(III) chloride) - were studied. (docphin.com)
  • compounds
  • If you are labelling with acid-sensitive compounds, labels must be attached in solution after cleavage & deprotection. (linktech.co.uk)
  • conjugation
  • We found that conjugation of cell penetrating peptide (CPP) to PNA is essential for the antimicrobial effect, with CPP1 [(KFF)3K] being the most effective against E. amylovora . (frontiersin.org)
  • genetic
  • Can pseudocomplementary peptide nucleic acid nucleases (pcPNANs) be a new tool for genetic engineering? (peerj.com)
  • genetic engineering , pseudocomplementary peptide nucleic acid nucleases , DNA double strand break , homologous recombination , error-prone nonhomologous end joining. (peerj.com)
  • Shi P. ( 2014 ) Can pseudocomplementary peptide nucleic acid nucleases (pcPNANs) be a new tool for genetic engineering? (peerj.com)
  • In this paper, we propose a new tool for genetic engineering, the pseudocomplementary peptide nucleic acid nucleases (pcPNANs), which are composed of a pseudocomplementary PNA (pcPNA) specific for a DNA target sequence, a FokI nuclease cleavage domain and a nuclear localization signal. (peerj.com)
  • distinct
  • We have sought to converge the two distinct fields of DNA and peptide self-assembly by using an artificially synthesised polymer that has attributes of both chemical families. (esrf.eu)
  • receptor
  • Due to the presence of thymine bases and peptide bonds, an interaction between Hg(2+) ion and receptor layer occurs. (docphin.com)
  • application
  • Global Peptide Nucleic Acids Market: Product Segment Analysis Double Chain Single Chain Type 3 Global Peptide Nucleic Acids Market: Application Segment Analysis Gene Chip Electrophoresis Others Reasons for Buying this Report This report provides pin-point analysis for changing competitive dynamics It provides a forward looking perspective on different factors driving or restraining market growth It provides a six-year forecast assessed on the basis. (researchbeam.com)
  • specific
  • Pipkorn R, Wiessler M, Waldeck W, Hennrich U, Nokihara K, Beining M, Braun K. Improved Synthesis Strategy for Peptide Nucleic Acids (PNA) appropriate for Cell-specific Fluorescence Imaging. (medsci.org)
  • Feasible patient-specific theranostic platforms in the personalized medicine are particularly dependent on efficient and clinically applicable peptide constructs. (medsci.org)
  • base
  • The very unique architecture that results from both the peptide-like and DNA base pairing results also in unpredicted physical properties. (esrf.eu)
  • properties
  • A new family of nanostructures sharing properties of both peptide and DNA was discovered. (esrf.eu)
  • Furthermore, peptide assemblies have been shown to exhibit remarkable physical properties including high mechanical rigidity, luminescence, piezoelectricity, and semiconductivity . (esrf.eu)