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  • detection
  • Herein, we developed a sensitive PCR-free electrokinetic-based sensor combined with a customized bis-peptide nucleic acid (bis-PNA) and gamma-PNA (γ-PNA) probes immobilized on beads, for the detection of mtDNA point mutations and sequence-specific supercoiled plasmid DNA at the picomolar range. (springer.com)
  • Coupling either one of these separation techniques, SEC or FFF, with MALS, specific viscosity detection and DLS delivers very interesting and valuable results in terms of macromolecular separation and characterization- SEC-MALS-DLS-IV or FFF-MALS-DLS-IV . (news-medical.net)
  • covalent
  • Ultimately, a rational structure-based design strategy was successful for the identification of Siah inhibitors in which peptide binding drives specific covalent bond formation with the target. (jove.com)
  • sequence
  • Ausec L, Črnigoj M, Šnajder M, Ulrih NP, Mandic-Mulec I (2015) Characterization of a novel high-pH-tolerant laccase-like multicopper oxidase and its sequence diversity in Thioalkalivibrio sp. (springer.com)
  • The Lm agr system is specific for this pentapeptide since the AIP of Lactobacillus plantarum , which also is a pentapeptide yet with different amino acid sequence, did not induce P II activity. (frontiersin.org)
  • backbone
  • A synthetic nucleic acid analog in which natural nucleotide bases are linked to a peptide-like backbone instead of the sugar-phosphate backbone found in DNA and RNA. (thefreedictionary.com)
  • They reveal similarities to the native nucleic acids whose phospho-ribose backbone is substituted by a backbone of poly-2-aminoaethyl glycine with nucleobases connected via an acetate linker [ 31 - 34 ]. (medsci.org)
  • Peptide nucleic acid (PNA) is a chimeric molecule that consists of hydrogen-bonding purine and pyrimidine heterocycles attached to a pseudopeptide backbone. (schoolbag.info)
  • However, one of the most radical structural modifications of DNA to appear in the literature is peptide nucleic acid (PNA, Fig. 1), in which the sugar-phosphate backbone is abolished entirely in favor of a pseudopeptide. (schoolbag.info)
  • complementary
  • The speed of the Orbitrap Elite furthermore allows scan modes in which complementary dissociation mechanisms are routinely obtained of all fragmented peptides. (mcponline.org)
  • genome
  • We are currently looking into designing small molecules such as specific peptide nucleic acids , which are stable and can be easily delivered to block the cancer-driven RNA editing process, said Prof Tan, who is also from the Duke-NUS Medical School and Genome Institute of Singapore (GIS). (thefreedictionary.com)
  • Belov K, Sanderson CE, Deakin JE et al (2007) Characterization of the opossum immune genome provides insights into the evolution of the mammalian immune system. (springer.com)
  • structural
  • Dr. Giovanni Gadda's research focus is on the mechanistic, biochemical and structural characterization of enzymes that catalyze oxidation-reduction reactions. (gsu.edu)
  • fatty
  • Instead, they house a hydrophobic tunnel and hydrophilic chamber that are occupied by fatty acid, which engages a conserved arginine and chloride ion via its carboxyl head group. (jove.com)
  • In vivo, these domains may therefore recognize changes in fatty acid synthesis, chloride ion concentration, and/or pH. (jove.com)
  • Chemistry
  • In this context our modified solid phase peptide chemistry using temperature shifts during synthesis is considered as an appropriate technology. (medsci.org)
  • In general, an appropriate solid support must be cleavable in acid, leaving a C-terminal amide, and be compatible with Fmoc chemistry. (linktech.co.uk)
  • receptor
  • We have recently demonstrated that the receptor for parathyroid hormone (PTH) and PTH-related peptide (PTHrP), PTHR, can be localized to the nucleus of cells within the liver, kidney, uterus, gut, and ovary of the rat. (biomedsearch.com)
  • base
  • Further, this bioanalytical tool can open up a new paradigm in clinical settings for the screening of double-stranded circular nucleic acids with a single-base mismatch specificity in a rapid and sensitive manner. (springer.com)
  • analysis
  • Analysis of supernatants of a recombinant Escherichia coli strain expressing AgrBD identified a peptide identical in mass and charge to the cyclic pentapeptide. (frontiersin.org)
  • expression
  • The Lm agr operon is homologous to the Staphylococcus aureus system, which includes an agrD -encoded autoinducing peptide that stimulates expression of the agr genes via the AgrCA two-component system and is required for regulation of target genes. (frontiersin.org)
  • cells
  • After ligation to the modular peptide-based BioShuttle carrier, which was utilized to facilitate the delivery of the functional modules into the cells' cytoplasm, the modules were scrutinized. (medsci.org)
  • Andersson M, Karlsson-Sjöberg J, Pütsep KL (2012) CRS-peptides: unique defense peptides of mouse Paneth cells. (springer.com)
  • signal
  • Bagos PG, Nikolaou EP, Liakopoulos TD, Tsirigos KD (2010) Combined prediction of Tat and Sec signal peptides with hidden Markov models. (springer.com)
  • Of the four proteins encoded by the agr operon, AgrB is a membrane-bound peptidase that cleaves and processes the agrD -derived propeptide at the C-terminus, catalyzes formation of a thiolactone ring with a central cysteine, and, in combination with the signal peptidase SpsB, effects export and release of the active autoinducing peptide (AIP). (frontiersin.org)