AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
MethylationPeptidesDNA MethylationCpG IslandsPeptide FragmentsMolecular Sequence DataAmino Acid SequenceEpigenesis, GeneticBase SequenceBinding SitesPromoter Regions, GeneticPeptide LibraryModels, MolecularDNA (Cytosine-5-)-MethyltransferaseSulfitesProtein BindingProtein ConformationAntimicrobial Cationic PeptidesCytosineLysineAzacitidineMethyltransferasesPeptides, CyclicProtein Structure, TertiaryGene SilencingMutagenesis, Site-DirectedMutationHistonesPeptide MappingSequence Homology, Amino AcidProtein Structure, SecondaryKineticsDNA Modification MethylasesStructure-Activity RelationshipOligopeptidesCell LineDNA5-MethylcytosineSubstrate SpecificityEscherichia coliGenomic ImprintingHistone-Lysine N-MethyltransferaseRecombinant ProteinsAmino AcidsCloning, MolecularEpigenomicsSequence AlignmentDNA-Cytosine MethylasesProtein MethyltransferasesLong Interspersed Nucleotide ElementsS-AdenosylmethionineAmino Acid SubstitutionChromatography, High Pressure LiquidCysteineMagnetic Resonance SpectroscopyPolymerase Chain ReactionMass SpectrometrySequence Analysis, DNAProtein-Arginine N-MethyltransferasesProteinsProtein Processing, Post-TranslationalNatriuretic Peptide, BrainDinucleoside PhosphatesDNA PrimersCell Line, TumorBacterial ProteinsCrystallography, X-RayTranscription, GeneticArginineTrypsinRNA, MessengerRecombinant Fusion ProteinsConserved SequenceElectrophoresis, Polyacrylamide GelAmino Acid MotifsCattleVasoactive Intestinal PeptideDNA, NeoplasmEpitopesPeptide BiosynthesisGene Expression Regulation, NeoplasticCell-Penetrating PeptidesMolecular WeightCalcitonin Gene-Related PeptideMembrane ProteinsCells, CulturedGene Expression RegulationProtein PrecursorsCatalytic DomainDNA-Binding ProteinsS-AdenosylhomocysteineCatalysisHistidineHydrogen-Ion ConcentrationChromatinCarrier ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationDeoxyribonuclease HpaIIGenes, p16Peptide Hydrolases
Lysine residuesProteinsAcetylationPhosphorylationModificationsSynthesized histone H3Arginine methylationProline residuesAssaysHistonesGeneVice versaMoleculesSequenceH3K9HydrophobicMethylatesSubstitutionsAmideTumorPatternsOligomersBindNascentNovoFormaldehydeChromatin structurePTMsStabilityIntracellularMetabolicMutationsDifferentiationSequencesApproachBiologicalImportantlyNuclearGenomeFunctionalTandemDependentPromotesAnalysisMass
Lysine residues9
Proteins12
  • In this study, we adopted several strategies to address this key question including: a detailed characterization of mice with cell-lineage-specific ablation of in immature oligodendrocyte progenitors or in oligodendrocytes, the use of CRISPR/Cas9 and pharmacological inhibitors to interfere with PRMT5 function in primary OPC cultures, transcriptomic analyses, and biochemical assays using synthetic proteins and modified histone peptides. (hwupdate.org)
  • Lysine methylation marks manifest their biological effect via so-called 'readers' (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. (europa.eu)
  • Here, a genome-wide analysis of the human mitochondrial transcriptome shows that 2'- O -methylation is limited to residues of the mitoribosomal large subunit (mtLSU) 16S mt-rRNA, introduced by MRM1, MRM2 and MRM3, with the modifications installed by the latter two proteins being interdependent. (nature.com)
  • Caspase 4 drug Medium-length peptides often bind tightly and particularly to companion proteins, which enables these peptides to serve as agonists or antagonists of biological signalling pathways which can be hard to modulate with compact molecules. (emlinhibitor.com)
  • We've got been making use of -helical BH3 domains derived from pro-apoptotic BH3-only proteins as a model system for exploring the effects of incorporating -amino acid residues into synthetic peptidic oligomers [4b, 4c, 5]. (emlinhibitor.com)
  • BH3 domains are brief segments (about 15 -amino acid residues) that engage a sizable hydrophobic groove on pro-survival Bcl-2 loved ones proteins [5b, 6]. (emlinhibitor.com)
  • Here we describe detailed protocols to process relatively small amounts of mouse liver tissue that integrate isolation of proteins, proteolytic digestion into peptides, immunoaffinity enrichment of acetylated peptides, identification of acetylation sites, and comprehensive quantification of relative abundance changes for thousands of identified lysine acetylation sites. (escholarship.org)
  • D) Specificity of the anti-eEF1AK55me2 antibody in dot blot assays using biotinylated eEF1AK55me2 peptides and 19 different peptides from your indicated proteins that harbor a dimethyl lysine. (epf2013.org)
  • Molecular and Structural Parallels between Gluten Pathogenic Peptides and Bacterial-Derived Proteins by Bioinformatics Analysis. (uni-bielefeld.de)
  • Phosphorylation of serine residues within Histone H3 is a highly dynamic process that associates with acetylation and methylation to create combinatorial patterns which are read by specific detector proteins (A. Sawicka & C. Seiser (2012) Biochimie 94, 2193-2201). (eurogentec.com)
  • Isoprenylcysteine carboxyl methyltransferase (ICMT) methylesterifies C-terminal prenylcysteine residues of CaaX proteins and some RAB GTPases. (sdbonline.org)
  • A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
Acetylation4
Phosphorylation4
Modifications8
  • The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Because the study of symmetric arginine methylation relies on the high quality of reagents, in this study we extensively characterized the specificity of all the commercially available antibodies to study this modification and selected those with the highest level of discriminatory power from other modifications (including asymmetric methylation at the same residue). (hwupdate.org)
  • DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
  • Here, we summarize the different types of epigenetic enzymes which target corresponding protein domains, emphasize DNA methylation , histone modifications, and microRNA-mediated cooperation with epigenetic modification, and highlight recent achievements in developing targets for epigenetic inhibitor therapy. (frontiersin.org)
  • The Speedy 28-day polyclonal antibody recognizes Histone H3 K9me2 (E), independently of neighboring modifications S10ph and K14ac marks (H,I). The antibody specificity has been determined with different peptides derived from histone H3 (amino acids 1-20). (eurogentec.com)
  • Dynamic changes in histone modifications precede de novo DNA methylation in oocytes. (crbdiscovery.com)
  • Cell reprogramming entails epigenetic changes by chromatin redesigning, histone modifications, and DNA methylation for regular maintenance and advancement of mobile differentiation [3, 15]. (palomid529.com)
Synthesized histone H31
  • Synthesized histone H3 peptide corresponding to residues within 1-21 of human histone H3, biotin conjugated. (epigentek.com)
Arginine methylation1
  • Another paper explains the effects of arginine methylation on biochemical functions such as transcription activation and repression, mRNA splicing, nuclear-cytosolic shuttling, and DNA repair. (wikipedia.org)
Proline residues2
  • The presence of proline residues on the carboxy side of the peptide bond inhibits the cleavage. (serva.de)
  • John T, Pires E, Hester SS, Salah E, Hopkinson RJ, Schofield CJ Formaldehyde reacts with N-terminal proline residues to give bicyclic aminals. (le.ac.uk)
Assays3
  • Molecular features of ligand binding to MHC class II HLA-DR molecules have been elucidated through a combination of peptide structure-activity studies and structure-based drug design, resulting in analogues with nanomolar affinity in binding assays. (rcsb.org)
  • The proposal will apply an interdisciplinary approach in an international environment to maximize its chances of success: the biological role of reading domains will be evaluated with molecular biology (histone and chromatin pulldowns, ChIP-seq, confocal microscopy), biochemistry (Peptide arrays, mass spectrometry, methyltransferase activity assays) and biophysics techniques (fluorescence anisotropy, circular dichroism spectroscopy) as well as crystallography (solving the structure of reading domains). (europa.eu)
  • Up to a 40-fold difference in potency resulted for the β-MePhe 7 stereoisomeric peptides using the linear template in these assays. (umn.edu)
Histones1
  • Due to histones containing a large number of lysine and arginine residues they have a positive net charge which interacts in an electrostatic manner with the negatively charged phosphate groups in DNA. (crbdiscovery.com)
Gene7
Vice versa2
  • Here, we show that specific canonical histone marks interfere with Ser-ADPr of neighboring residues and vice versa. (shengsci.com)
  • From celiac disease to coccidia infection and vice-versa: The polyQ peptide CXCR3-interaction axis. (uni-bielefeld.de)
Molecules4
  • Peptide and peptide mimetic inhibitors of antigen presentation by HLA-DR class II MHC molecules. (rcsb.org)
  • These studies illustrate the complementary roles played by phage display library methods, peptide analogue SAR, peptide mimetics substitutions, and structure-based drug design in the discovery of inhibitors of antigen presentation by MHC class II HLA-DR molecules. (rcsb.org)
  • The complex molecules are preferably peptide conjugates having improved cell-permeability, serum stability and kinase selectivity compared to known protein kinase inhibitors. (justia.com)
  • The present invention relates to cell permeable, stable conjugates comprising a cell-permeability enhancement moiety and a peptide or peptidomimetic, as selective inhibitors of protein kinases, to pharmaceutical compositions containing them, as well as to processes for the preparation and use of such complex molecules. (justia.com)
Sequence4
  • Due to its highly specific cleavage of peptides Glu-C is used in proteomics for peptide mapping and protein sequence work. (serva.de)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • Arabidopsis thaliana methylation pattern analysis based on ONT sequence reads. (uni-bielefeld.de)
  • Search our datasheets by catalogue number, peptide sequence or name. (crbdiscovery.com)
H3K91
  • Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
Hydrophobic1
  • The peptide exit tunnel is lined with hydrophobic residues, which stabilise the highly hydrophobic nascent mitochondrial peptides 4 . (nature.com)
Methylates2
  • METTL13 Methylates eEFlAK55 methylation with recombinant METTL13 using deuterated SAM like a methyl donor. (epf2013.org)
  • DNMT1 methylates CpG residues, with a preference for hemimethylated DNA, and associates with DNA replication sites in S PHASE to maintain the methylation pattern in the newly synthesized strand, which is essential for EPIGENETIC PROCESSES. (bvsalud.org)
Substitutions2
  • Nonetheless, our initial reports utilising / BH3 domain peptides having a 1:1 alternation of and cyclic substitutions demonstrated that important side-chain interactions expected for engaging anti-apoptotic. (emlinhibitor.com)
  • This default peptide view shows a histogram of single base substitutions, colour coded by residue according to the colour scheme used in Ensembl. (sanger.ac.uk)
Amide3
  • A different approach to improve peptide stability entails alterations for the -peptide backbone like backbone amide methylation [3] and incorporation -amino acids [4]. (emlinhibitor.com)
  • Additionally, this peptide contains an uncharged C-terminal amide. (crbdiscovery.com)
  • This peptide also has an uncharged C-terminal amide. (crbdiscovery.com)
Tumor3
  • Expression cells, which may be ovarian or ciliary, control circled to the particle peptide through building with membranes and rely bound or lagging chains or non-processive Groups in the covalent periods of the kinase tumor. (familie-vos.de)
  • Lysine methylation-dependent binding of 53BP1 to the pRb tumor suppressor. (ox.ac.uk)
  • We did simultaneous introduction of two tumor suppressor peptides (p14 ARF and p16 INK4a or p16 INK4a and p21 CIP1 functional peptides) compared with single-peptide introduction using Wr-T-mediated peptide delivery. (aacrjournals.org)
Patterns1
Oligomers1
  • Oligomers of Aβ peptide are implicated as the most probable causative agent in Alzheimer's disease. (nature.com)
Bind3
  • Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)
  • Indeed, modified residues close to one PTM may prevent the PTM-specific antibody to bind its epitope. (eurogentec.com)
  • A polyclonal antibody targeting the dimethyl-Lysine 9 of Histone 3 was tested against various H3 peptides and shown to bind to all peptides containing the dimethyl-Lysine 9, no matter the surrounding residues. (eurogentec.com)
Nascent1
  • Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. (cipsm.de)
Novo1
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
Formaldehyde2
  • Generation of 14C-labelled formaldehyde was verified using TLC, HPLC, HPLC-MS and HPLC-MS-MS. One part of the generated formaldehyde was urinary eliminated, an another part took place in the methylation reaction of L-lysine giving 14C-labelled N-E-monomethyl lysine. (otka-palyazat.hu)
  • Only a minor part of formaldehyde was subjected to N-methylation of lysine. (otka-palyazat.hu)
Chromatin structure1
PTMs1
  • PTMs are specific to types of amino acid residues. (deepdyve.com)
Stability1
  • Various methods happen to be employed to enhance the metabolic stability of peptides whilst retaining their protein-binding profiles. (emlinhibitor.com)
Intracellular2
  • The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)
  • Intracellular delivery of multiple functional peptides forms a basis for potent, non-invasive mode of delivery, providing distinctive therapeutic advantages. (aacrjournals.org)
Metabolic1
  • Methionine can then be used to produce SAM, the body's universal methyl donor, which participates in several other key metabolic pathways, including the methylation of DNA and myelin. (medscape.com)
Mutations1
  • [ 21 ] Mutations in either HuR or TERC can weaken the HuR- TERC binding and reduce TERC methylation, resulting in decreased telomerase activity. (medscape.com)
Differentiation1
  • 10] reported that the K36M mutation of H3 impairs the differentiation of mesenchymal progenitor cells and promotes undifferentiated sarcoma through altered histone methylation landscape. (deepdyve.com)
Sequences2
  • The glycine-rich C terminus has repeat GRG sequences, which has been shown to be susceptible to methylation of the arginine, either symmetrically or asymmetrically. (wikipedia.org)
  • The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
Approach2
  • Peptide-sugar hybrids have also been identified using a structure-based design approach in which the sugar residue replaces a dipeptide. (rcsb.org)
  • Finally, we facilitate wider investigations into the interplay of histone marks with Ser-ADPr by introducing a simple approach for profiling posttranslationally modified peptides. (shengsci.com)
Biological1
  • antibody, raised against a peptide harboring this PTM only, may not work in a biological sample. (eurogentec.com)
Importantly2
  • Importantly, the KMET-READ project will also develop a yeast-3-hybrid method for the identification of new reading domains, which will allow to discover binding partners for just recently characterized new protein methylation marks. (europa.eu)
  • More importantly, we found a global decrease of multiply acetylated histone H4 peptides. (biomedcentral.com)
Nuclear1
  • Residues 173-176 are predicted to be a nuclear localization signal KKLK (K-[K/R]-X-[K/R]). FAM98A has two paralogs: FAM98B and FAM98C. (wikipedia.org)
Genome1
  • To view all methylation probes within or in close proximity to RPS17 please use the COSMIC Genome Browser . (sanger.ac.uk)
Functional1
Tandem2
  • Structural elucidation of 53BP1 in complex with a methylated K810 pRb peptide emphasized the role of the 53BP1 tandem tudor domain in recognition of the methylated lysine and surrounding residues. (ox.ac.uk)
  • B) Representative tandem mass spectra identifying mono- (top) and di- (bottom) methylation of eEF1AK55 by recombinant METTL13 using deuterated SAM and digested with trypsin. (epf2013.org)
Dependent1
  • Significant differences in prolonged (residual) activities were also observed for these modified peptides and were dependent upon stereochemistry of the β-methyl amino acid, peptide template, and bioassay system. (umn.edu)
Promotes1
Analysis2
Mass1
  • The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)