The set consisted of short overlapping peptides derived from the protein being investigated and the peptides were captured onto plate wells coated with streptavidin before completing an ELISA with each monoclonal antibody (Figure 1). (mimotopes.com)
Proteins5
Sets of overlapping peptides from potential target vaccine proteins are screened with immune sera or cells to find vaccine or diagnostic test candidates. (mimotopes.com)
We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
Peptides mimicking protein activity can overcome the perceived limitations of therapeutic proteins. (ox.ac.uk)
The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
Protein9
In a typical example, a library of monoclonal antibodies to a small protein, thought to recognize linear epitopes, was screened using a PepSet of biotinylated peptides (Figure 2). (mimotopes.com)
Peptide mapping, a bottom-up approach to protein characterization, enables the profiling of a protein's primary structure as well as its post-translational modifications (PTMs) such as oxidation, deamidation, and glycosylation. (sigmaaldrich.com)
The use of a high-resolution mass spectrometry-based approach for the characterization is commonly referred to as a multi-attribute method (MAM) 1 , as the peptide mapping analysis yields multiple pieces of information about the protein in a single analysis. (sigmaaldrich.com)
A highly specific protease, trypsin, is used to cleave the protein into suitably sized peptides, although a combination of multiple proteases may also be used to generate more complete PTM site information. (sigmaaldrich.com)
We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. (elifesciences.org)
Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
Tryptic3
Here, we provide an optimized method for tryptic digestion and peptide mapping using adalimumab as an example. (sigmaaldrich.com)
iii), identify exposed regions of the intact and truncated TIMP-1 molecules by measuring the rate of tryptic peptide release and (iv), locate sites of aberrant proteolysis seen when recombinant human TIMP-1 was purified at large scale. (kent.ac.uk)
SF samples from end-stage knee osteoarthritis patients undergoing total knee replacement surgery and controls, that is, deceased donors without known knee disease were previously run using liquid chromatographymass spectrometry (LC-MS). This data was used to perform new database searches generating results for non-tryptic and semi-tryptic peptides for studies of degradomics in OA. (lu.se)
Amino1
This procedure accounted for the entire molecule, except two short peptides of 2 and 4 amino acids in length. (kent.ac.uk)
Antibodies1
Probing the wells with antibody and washing away unbound antibodies quickly reveals those peptides to which the antibody has bound. (mimotopes.com)
Sequences4
When a bioactive sequence is already known, analoging studies can be carried out using the set of peptides comprising single-point substitution sequences, or a combination of 2 or more selected substitutions. (mimotopes.com)
Sequences exhibiting enhanced bioactivity, or inhibitory properties, are candidates for development as peptide or peptidomimetic drugs. (mimotopes.com)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
Cleave1
Known proteolytic events (from the MEROPS peptidase database) were mapped to the dataset, allowing the identification of potential proteases and which substrates they cleave. (lu.se)
Antibody epitope mapping by ELISA is simple to perform using biotinylated peptides because the peptides can be captured onto Streptavidin™ or NeutrAvidin™ coated plates through the strong noncovalent avidin-biotin binding. (mimotopes.com)
Custom synthesized biotinylated PepSets for linear antibody mapping are designed by the research scientist with help from experienced Technical Consultants at Mimotopes, and are shipped either ready to redissolve and plate or as ready-to-use plates already coated with the peptide (Figure 2). (mimotopes.com)
Digestion2
Following digestion, peptides and glycopeptides are separated and analyzed by RP-LC-MS.M. (sigmaaldrich.com)
The filter membrane retains the mAb of interest until the digestion procedure is complete, following which peptides can pass through the filter while excluding trypsin. (sigmaaldrich.com)
Characterization2
Rodda, S.J. (2002) Peptide Libraries for T-Cell Epitope Screening and Characterization. (mimotopes.com)
Identification and characterization of the chemokine-binding interface of evasins could thus inspire the development of novel anti-inflammatory peptides that therapeutically target the chemokine network in inflammatory diseases. (ox.ac.uk)
Linear1
We used linear mixed models to estimate differences in peptide-level expression between the two groups. (lu.se)
Length1
T-cell epitopes (Helper and Cytotoxic) are easily located using PepSets provided peptides of the correct length are used2. (mimotopes.com)
Analysis1
To determine the suitability of an instrument for RPLC-MS analysis, a mixture of 14 isotopically labeled peptides is analyzed as a system suitability test prior to each run. (sigmaaldrich.com)
Sites2
The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
Cooperative phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide phosphorylated on both sites, as opposed to singly phosphorylated peptides. (nih.gov)
Study1
This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
Method1
The new mapping method could be used in the future to analyse the anatomy of different cell types, such as nerve cells and cells of the immune system. (elifesciences.org)
Interface1
We used hydrogen-deuterium exchange mass spectrometry to map the binding interface of the evasin P672 that physically interacts with C-C motif chemokine ligand 8 (CCL8) and synthesized a 16-mer peptide (BK1.1) based on this interface region in evasin P672. (ox.ac.uk)
Events1
We also developed a peptide-centric R tool, proteasy, which facilitates analyses that involve retrieval and mapping of proteolytic events. (lu.se)
Mass1
The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)
Cell1
This application is summarized in a separate article entitled Truncated Peptide Libraries for Cytotoxic T-Cell Epitope Mapping, available on the Mimotopes website. (mimotopes.com)