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  • HPLC
  • In fact, it can produce exceptional analytical results two to three times faster than conventional peptide mapping with fully porous HPLC columns, which can take up to 60 minutes. (technologynetworks.com)
  • The purpose of this study is to provide guidelines for developing optimal LC running conditions with Aeris™ PEPTIDE core-shell HPLC/UHPLC columns and determine the feasibility of transferring a method developed on UV to MS simply by adjusting the acidic modifier in the mobile phase. (advion.com)
  • bonds
  • This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. (wikipedia.org)
  • The trans form is preferred overwhelmingly in most peptide bonds (roughly 1000:1 ratio in trans:cis populations). (wikipedia.org)
  • Both of these mechanisms for lowering the activation energy have been observed in peptidyl prolyl isomerases (PPIases), which are naturally occurring enzymes that catalyze the cis-trans isomerization of X-Pro peptide bonds. (wikipedia.org)
  • Nevertheless, peptide bonds can undergo chemical reactions, usually through an attack of an electronegative atom on the carbonyl carbon, breaking the carbonyl double bond and forming a tetrahedral intermediate. (wikipedia.org)
  • MALDI
  • citation needed] A small fraction of the peptide (usually 1 microliter or less) is pipetted onto a MALDI target and a chemical called a matrix is added to the peptide mix. (wikipedia.org)
  • Matrix and peptide molecules co-crystallize on the MALDI target and are ready to be analyzed. (wikipedia.org)
  • In matrix-assisted laser desorption ionization (MALDI), a fragmented peptide sample is loaded onto a matrix and ionized through the use of a high energy laser. (wikipedia.org)
  • residues
  • The peptide was sequenced yielding the sequence TVTDIKDLTK24LGAIY - TK24L, which contain the crosslinked residues K24 and K24 suggesting the proximity between both N-terminal ends of the polypeptides. (nih.gov)
  • biopharma
  • The Thermo Scientific Q Exactive BioPharma Platform of Orbitrap-based mass spectrometers add superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. (thermofisher.com)
  • Analysis
  • When a similar analysis of the crosslinked protein was carried out using trypsin, a large peptide (m/z nearly 5,000) was observed (Figure 7A) that would correspond to crosslinking between K31 and either K20 or K24 indicated for the mass of the fragment (bioworks software analysis) of 4999.5366 m/z. (nih.gov)
  • Additional analysis tools such as BLAST searches, genome maps, genome or gene alignment, phylogenetic trees, etc. are provided. (wikipedia.org)
  • consists
  • Cupiennin 1 family is the more well-known group, and consists of at least four peptides, such as cupiennin 1a, 1b, 1c, and 1d. (wikipedia.org)
  • identify
  • Here, we identify the metal abstraction peptide (MAP) as an inhibitor of MMP-8 and demonstrate that tethering MAP to methacrylate polymers effectively inhibits catalysis. (ku.edu)
  • In biological applications, benzophenones have been used extensively as photophysical probes to identify and map peptide-protein interactions. (wikipedia.org)
  • significantly
  • One major advantage to mass fingerprinting is that it is significantly faster to carry out than peptide sequencing, yet the results are equally useful. (wikipedia.org)
  • A nonnative isomer of some peptide groups can disrupt the conformational folding significantly, either slowing it or preventing it from even occurring until the native isomer is reached. (wikipedia.org)
  • Search
  • Visualize, interrogate, and validate search results with interactive MS/MS, EIC & MS1 charts and protein/peptide table lists. (massmatrix.bio)
  • Once formed, a peptide-mass fingerprint can be used to search in databases for related protein or even genomic sequences, making it a powerful tool for annotation of protein-coding genes. (wikipedia.org)
  • Popular
  • He also developed and published a peptide fingerprinting technique that was so popular that it became a citation classic Cleveland did postdoctoral work with William J. Rutter at the University of California at San Francisco from 1978 to 1981. (wikipedia.org)
  • results
  • Even then, if reliable results are obtained, there must be a matching peptide sequence in the database you are searching in order for the results to be useful. (wikipedia.org)
  • Earlier
  • Earlier, Hugh O. McDevitt and his coworkers mapped an Immune response-1 (Ir-1) locus influencing the level of antibody production against the synthetic polypeptide (T,G)-L-A into the Mhc. (wikipedia.org)
  • In fact, members of this family were discovered earlier in 1994 as member of the CSTX peptides. (wikipedia.org)
  • bond
  • Uses proteinase K kits to cleave the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. (thermofisher.com)
  • When two amino acids form a dipeptide through a peptide bond it is called condensation. (wikipedia.org)
  • The formation of the peptide bond consumes energy, which, in living systems, is derived from ATP. (wikipedia.org)
  • A peptide bond can be broken by hydrolysis (the addition of water). (wikipedia.org)
  • The wavelength of absorption A for a peptide bond is 190-230 nm (which makes it particularly susceptible to UV radiation). (wikipedia.org)
  • However, the activation energy can be lowered (and the isomerization catalyzed) by changes that favor the single-bonded form, such as placing the peptide group in a hydrophobic environment or donating a hydrogen bond to the nitrogen atom of an X-Pro peptide group. (wikipedia.org)
  • Due to its resonance stabilization, the peptide bond is relatively unreactive under physiological conditions, even less than similar compounds such as esters. (wikipedia.org)
  • groups
  • However, X-Pro peptide groups tend to have a roughly 30:1 ratio, presumably because the symmetry between the C α {\displaystyle \mathrm {C^{\alpha }} } and C δ {\displaystyle \mathrm {C^{\delta }} } atoms of proline makes the cis and trans isomers nearly equal in energy (See figure, below). (wikipedia.org)
  • nonnative isomers of other peptide groups may not affect folding at all. (wikipedia.org)
  • identification
  • With the emergence of new protein therapeutics, including biosimilars, biobetters and ADCs, peptide mapping by LC/MS is becoming more commonly used for the identification of post-translational modifications (PTMs), glycosylation, and conjugation sites, as well as primary sequence confirmation. (advion.com)
  • sample
  • Because of this sensitivity, sample preparation is likely the most important step in forming a peptide-mass fingerprint. (wikipedia.org)
  • matrix
  • The matrix molecules are required for the desorption of the peptide molecules. (wikipedia.org)
  • The energy transfer is sufficient to promote the ionisation and transition of matrix molecules and peptides from the solid phase into the gas phase. (wikipedia.org)
  • However
  • However the AppliedBiosystems MAP substrate for peptide synthesis has been suggested, but not by anyone with much experience using it. (bio.net)
  • shows
  • The image shows the ionization and sequence of the LC peak (indicated in the inset) corresponding at the intermolecular peptide 67-73 and 86-95 with K68 and K88 crosslinked with BS3. (nih.gov)
  • App ID # 18764 shows the Lys-C/ Asp-N peptide map run on a compared to the Kinetex™ 2.6 µm C18 column to show the high resolution that Kinetex delivers in separating peptide. (phenomenex.com)