• Proteome-derived peptide libraries are generated by trypsin, GluC, or chymotrypsin digestion of biologically relevant proteomes, such as cytosolic lysates, to generate three separate libraries that each differ from the others in their C-terminal amino acid residues according to the protease specificity. (springer.com)
  • Primary amines of all peptides are then chemically protected so that after incubation with a test protease, the neo-N-termini of the prime-side cleavage products with exposed α-amines can be specifically biotinylated, enriched, and identified by liquid chromatography-tandem mass spectrometry. (springer.com)
  • Herein we describe a simplified procedure to produce PICS peptide libraries, the methods to perform a PICS assay, and a new method of data analysis. (springer.com)
  • The Alanine Scanning Library can be used to determine the relative importance of each substituted residues toward the original peptide's functionality since knocked off key residues reflect diminished peptide activity. (genscript.com)
  • The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. (diva-portal.org)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. (osti.gov)
  • Over years, we have integrated multiple peptide libraries with varied lengths and properties to satisfy all kind of applications. (labtube.tv)
  • The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. (neb.com)
  • Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events. (thermofisher.com)
  • All purified peptides are delivered with complete QC data including RP-HPLC, MS, and COA report to ensure high quality. (genemedsyn.com)
  • Authoritative and practical, Peptide Libraries: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies. (springer.com)
  • If none of the premade libraries meets your requirement, you are welcome to contact us, and we will be more than happy to create a custom library for you. (creative-biolabs.com)
  • Empowered by extensive experience and well-established phage display technique platform, Creative Biolabs now brings out a comprehensive list of premade peptide libraries for various research programs. (labtube.tv)
  • It's constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity. (genscript.com)
  • In order to elucidate the physiological function of the cell surface glycosoaminoglycan hyaluronan (HA), Mummert and co-workers (32) panned the Ph.D.-12 library against purified HA. (neb.com)
  • Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. (springer.com)
  • These random peptide libraries with such great quality and diversity can definitely make excellent tools for peptide agent discovery. (labtube.tv)
  • Usually, peptide library is synthesized on solid phase, mostly on resin, which can be made as flat surface or beads. (wikipedia.org)
  • If peptides do not pass these criteria, the customer will be informed and one resynthesis will be offered free of charge. (thermofisher.com)
  • The predominant selected peptide (Pep1) was determined to bind both free HA and HA expressed on cell surfaces, as well as to block HA binding to cell surfaces expressing the HA receptor, CD44. (neb.com)