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  • residue
  • having the library of different peptides at residue 2 and 3 would let you see if some change in chemical properties in the N-terminal tail of the ligated protein make the protein more useful or useful in a different way. (wikipedia.org)
  • Optional peptide modifications are available, including residue phosphorylation and acetylation, and different formulations are possible that enable study of cellular signaling events. (thermofisher.com)
  • Because of the specificity of the active site, OmpT does not act on peptides with a negatively charged residue adjacent to the scissile bond. (wikipedia.org)
  • During the proton transfer associated with the peptide cleavage, the negatively charged aspartate residue stabilizes the positively charged histidine. (wikipedia.org)
  • affinity
  • The technique enables the mass creation and interrogation of libraries via affinity selection, typically on an immobilized protein target. (wikipedia.org)
  • DEL libraries are subjected to affinity selection procedures on an immobilized target protein of choice, after which non-binders are removed by washing steps, and binders can subsequently be amplified by polymerase chain reaction (PCR) and identified by virtue of their DNA code (e.g.by DNA sequencing). (wikipedia.org)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • sequence
  • so far I have been unsuccessful in locating a suitable sequence and am hoping to broaden my search by using a panel of libraries. (bio.net)
  • No purification given, as peptide purity is sequence-dependent and a function of different liquid chromatographic conditions. (thermofisher.com)
  • For peptide synthesis ~2.4% of the peptides submitted, due to difficult sequence were unable to be synthesized. (thermofisher.com)
  • In this approach, a protein sequence database is used to calculate all putative peptide candidates in the given setting (proteolytic enzymes, miscleavages, post-translational modifications). (wikipedia.org)
  • The sequence search engines use various heuristics to predict the fragmentation pattern of each peptide candidate. (wikipedia.org)
  • Sequence database searching also demands vast search space, which still could not cover all possibilities of peptide dynamics, exhibiting limited efficiency post-translational modifications). (wikipedia.org)
  • Brenner and Janda suggested to generate individual encoded library members by an alternating parallel combinatorial synthesis of the heteropolymeric chemical compound and the appropriate oligonucleotide sequence on the same bead in a "split-&-pool"-based fashion (see below). (wikipedia.org)
  • synthetic
  • As the figure demonstrates the number of peptides formed in the synthetic process (3, 9, 27, 81) increases exponentially with the number of executed cycles. (wikipedia.org)
  • discovery
  • Chapter focus on methods and techniques for synthesis, genetic expression, hybrid synthesis-expression, examples of modern utility of these libraries, de novo discovery of reactions, hybrid organic-inorganic materials and, emerging tools for the analysis of these libraries by method of genetic selection and next-generation sequencing. (springer.com)
  • These random peptide libraries with such great quality and diversity can definitely make excellent tools for peptide agent discovery. (labtube.tv)
  • fragments
  • the mass spectra corresponding to the fragments of peptide precursor is recorded. (wikipedia.org)
  • Due to the complex nature of peptide fragmentation in a mass spectrometer, derivative fragmentation patterns fall short of reproducing experimental mass spectra, especially relative intensities among distinct fragments. (wikipedia.org)
  • chromatographic
  • Subsequently, one or more chromatographic separations are applied to resolve resulting peptides, which are then ionized and analyzed in a mass spectrometer. (wikipedia.org)
  • Novel
  • Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. (neb.com)
  • Functional
  • There is significant interest in the use of unmodified self-assembling peptides as building blocks for functional, supramolecular biomaterials. (rsc.org)
  • structural
  • citation needed] In order to handle the vast number of structural possibilities, researchers often create a 'virtual library', a computational enumeration of all possible structures of a given pharmacophore with all available reactants. (wikipedia.org)
  • Molecular
  • In this study, coarse grained molecular dynamics (CG-MD) simulations are used to understand the DPL results for F 2 , L 2 and mixed libraries. (rsc.org)
  • receptor
  • Human urotensin II (U-II), the most potent vasoconstrictor peptide identified to date, and its receptor (UT) are involved in etiology of hypertension. (ebscohost.com)
  • Most scorpions The box jellyfish Elapid snakes The cone snail The Blue-ringed octopus Venomous fish Frogs Palythoa coral Various different types of algae, cyanobacteria and dinoflagellates Myotoxins are small, basic peptides found in snake and lizard venoms, They cause muscle tissue damage by a non enzymatic receptor based mechanism. (wikipedia.org)
  • Another example is the μ-opioid receptor (MOR), which is bound and activated by the opioid peptide hormone β-endorphin. (wikipedia.org)
  • gene
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage by fusing the peptide of interest onto gene III of filamentous phage. (wikipedia.org)
  • small
  • Possible strategies towards this objective include increased sampling from diverse environments and application of metagenomics to identify bioactive compounds produced by currently unknown and uncultured microorganisms as well as the development of small-molecule libraries customized for bacterial targets. (wikipedia.org)
  • Type
  • Comparative value of Doppler echocardiography and B-type natriuretic peptide assay in the etiologic diagnosis of acute dyspnea. (annals.org)
  • In patients with acute dyspnea, how does the accuracy of Doppler echocardiography compare with bedside B-type natriuretic peptide (BNP) assay for diagnosing decompensated congestive left-heart failure (CHF)? (annals.org)
  • human
  • Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH). (rockland-inc.com)
  • drug
  • Arenicin is the name used to classify a group of antimicrobial peptides, which have shown promise as a novel drug to combat Gram-negative bacteria. (wikipedia.org)
  • cell
  • Peptides that were translocated across the outer membrane create pores in the cytoplasmic membrane, thereby disrupting the osmotic balance of the cell. (wikipedia.org)