• The 48HD ligand discovery technology is vital to the first step in this process as it screens the patient's tumor cells against large peptide libraries to identify the specific peptide ligands that bind to those cells. (globenewswire.com)
  • Small-molecule Peptide-Influenced Drug Repurposing (SPIDR) was developed to identify small molecule drugs that target a specific receptor by exploring the conformational binding space of peptide ligands. (biomedcentral.com)
  • Phage display is a widely-used tool for the directed evolution of peptide ligands from large combinatorial peptide libraries. (tamu.edu)
  • This method identifies peptide ligands as well as cytoxic peptides that may prove useful in developing anti-cancer agents. (peptidesguide.com)
  • Libraries in and of themselves can prove even more uniform than other methods in demonstrating full-length ligands during screening since molecular weight degeneracy can be eliminated right during the sequencing process versus during the synthesis, thus making the entirety of the technique much more simplified and less costly. (peptidesguide.com)
  • Noncognate or self peptide-MHC (pMHC) ligands productively interact with T-cell receptor (TCR) and are always in a large access over the cognate pMHC on the surface of antigen presenting cells. (edinst.com)
  • ProteoMiner protein enrichment technology employs a large bead-based library of combinatorial peptide ligands, which dilutes high-abundance proteins and concentrates low-abundance proteins. (bio-rad.com)
  • At least five subtypes of these libraries exist, and the distinguishing characteristic is the method of synthesis of each library. (wikipedia.org)
  • This form of peptide synthesis is limited to a peptide chain length of approximately 70 amino acids, and is generally unsuitable for the study of larger proteins. (wikipedia.org)
  • Large random peptide libraries are often used for the synthesis of certain peptide molecules, such as ultra-large chemical libraries for the discovery of high-affinity peptide binders, and an increase in the library size severely affects parameters, such as the synthesis scale, the number of library members, the sequence deconvolution and peptide structure elucidation. (wikipedia.org)
  • However, since there are 20 natural amino acids, the synthesis of a linear peptide ( n amino acids in length) can be produced in 20 n different combinations. (peptidesguide.com)
  • NovoPro has a rapid high-throughput parallel peptide synthesis platform that enables our customers to synthesize larger quantities of high purity peptides at lower cost than traditional methods. (novoprolabs.com)
  • With purified recombinant protein production and peptide synthesis to display limited/selected epitopes, intrinsic factors that can affect the functioning of resulting antibodies can be more easily selected for. (preprints.org)
  • The development of modern peptide chemistry has opened for the possibility of custom peptide synthesis that allows for systematically investigating the relationship between a specific oligopeptide molecular structure and the macroscopic phases and structures formed in such systems. (lu.se)
  • Recently, high-throughput DNA sequencing has enabled large-scale characterization of TCR sequences, producing detailed T cell repertoire (Rep-Seq) ( 11 ). (frontiersin.org)
  • Since the peptide CCS values are entirely determined by their linear amino acid sequences, they should be predictable with high accuracy and our deep learning model accurately predicted CCS values even for previously unobserved peptides. (news-medical.net)
  • The proteins that the phages are displaying can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those of other molecules. (wikipedia.org)
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • First, binding affinity: Good binding requires difficult peptide architectures and amino acid sequences that are perfectly complementary in shape and charge to the surface of their target proteins. (epfl.ch)
  • We use position-specific scoring matrices (PSSM) and these tensors to encode peptide sequences. (biomedcentral.com)
  • The PSSMs contain the evolutionary information of sequences, which contributes to reducing the impact of amino acid variations in peptide sequences. (biomedcentral.com)
  • Typically, nucleotide sequences encoding the protein/peptide library are fused to a gene encoding a phage coat protein thus allowing them to be displayed on the phage exterior. (cdc.gov)
  • We demonstrate the effectiveness of this approach by identifying drug-like, head-to-tail cyclic peptides that modulate the aggregation of the Alzheimer's disease-associated amyloid β peptide. (nature.com)
  • The use of this Group II of distinct 18 peptide mixtures, in combination with Group I of 180 distinct peptide mixtures (cat# 62017-1), can provide sequence preference information of different kinases. (anaspec.com)
  • Lead author Dr. Florian Meier, now an Assistant Professor in Functional Proteomics at the Jena University Hospital in Germany, said: "The scale and precision of peptide CCS values in our data from the timsTOF Pro was sufficient to train our deep learning model to accurately predict CCS values based only on the peptide sequence. (news-medical.net)
  • This connection between the amino acids contained within a peptide sequence and its measured CCS has tremendous potential to increase the confidence of protein identification. (news-medical.net)
  • Professor Fabian Theis stated: "Deep learning, in particular the used recurrent neural networks need a lot of samples to be predictive, so I was very happy when Matthias approached me and we jointly were able to predict and interpolate biochemical properties of peptides based only on their sequence. (news-medical.net)
  • The distinct activity of antimicrobial peptides governs their sequence, namely, the incorporation of these peptides into microbial cell membranes. (genscript.com)
  • 2) The influence of the peptide sequence and aromatic core of the building blocks. (europa.eu)
  • Because the four cysteines can be placed in many different ways along the sequence of amino acids, the double-bridging strategy allowed the researchers to generate an enormously large number of structurally diverse peptide architectures. (epfl.ch)
  • The scientists also found that the four cysteines could be bridged by two linkers in three different ways, giving rise to three different architectures from each single peptide sequence. (epfl.ch)
  • ACEP model can capture similarity between amino acids, calculate attention scores for different parts of a peptide sequence in order to spot important parts that significantly contribute to final predictions, and automatically fuse a variety of heterogeneous information or features. (biomedcentral.com)
  • Constructing and selecting complex sequence-based features that capture information about distal patterns within a peptide are used in the evolutionary feature construction method [ 17 , 18 ]. (biomedcentral.com)
  • This assists in knowing what is the most important within a sequence, thus avoiding the use of lengthy peptide chains. (peptidesguide.com)
  • I am using frag builder python module to generate peptide structures to compute the interaction energy for ensemble of peptides of a given sequence for a fixed bond lengths and bong angles. (stackexchange.com)
  • A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. (wiley.com)
  • This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. (wiley.com)
  • Although simple on a sequence level, there is a large amount of structural variation in coiled-coils. (lu.se)
  • We use structure based modeling and computational protein design methods to optimize the protein sequence of proteins and peptides in order to control their assembly. (lu.se)
  • Antibacterial drugs were originally isolated from natural sources, but as the threat of antibacterial resistance looms, the development of intelligently designed antimicrobial drugs, including small molecules and custom antimicrobial peptides has been fostered. (genscript.com)
  • We will make dynamic combinatorial libraries of macrocyclic molecules of different ring sizes that can exchange building blocks through reversible covalent chemistry. (europa.eu)
  • In this system, large combinatorial libraries of macrocyclic molecules are biosynthesized in Escherichia coli cells and simultaneously screened for their ability to rescue pathogenic protein misfolding and aggregation using a flow cytometric assay. (nature.com)
  • PubChem mostly contains small molecules, but also larger molecules. (mcmaster.ca)
  • Antimicrobial peptides (AMPs), effecter molecules of innate immune system, can defend host organisms against microbes and most have shown a lowered likelihood for bacteria to form resistance compared to many conventional drugs. (biomedcentral.com)
  • Jiang used synthesized strips of DNA molecules as templates to create a large library of peptide antigens in test tubes. (utexas.edu)
  • For example, a peptide chain of 10 residues in length is used in native chemical ligation with a larger recombinantly expressed protein. (wikipedia.org)
  • The peptide fills an alpha-BTX pocket made of residues located at fingers I and II, as well as at the C-terminal region. (rcsb.org)
  • Of the peptide residues, the largest contact area is formed by Tyr-3 and Tyr-4. (rcsb.org)
  • These findings are in accord with the previous data in which it had been shown that substitution of these aromatic residues by aliphatic amino acids leads to loss of binding of the modified peptide with alpha-BTX. (rcsb.org)
  • Further, a hexapeptide is a peptide that contains six amino acid residues. (peptidesguide.com)
  • Here huge numbers of peptides are screened so as to isolate and identify critical bioactive peptides that are used for new ways to cure diseases and cancers. (peptidesguide.com)
  • GPCR-Based Bioactive Peptide Screening Using Phage-Displayed Peptides and an Insect Cell System for Insecticide Discovery. (oregonstate.edu)
  • The fire ant is a good model system, since bioactive peptides have been identified for a specific GPCR. (oregonstate.edu)
  • The GPCR-based bioactive peptide screening method offers multiple advantages. (oregonstate.edu)
  • Delivery of bioactive peptides to target pests can be flexible, such as topical, ingestion, and plant-incorporated protectants. (oregonstate.edu)
  • The structure of the complex may explain the fact that the library-derived peptide binds alpha-BTX with a 15-fold higher affinity than that shown by the acetylcholine receptor peptide (alpha185-196). (rcsb.org)
  • The affinity of a TCR for a given peptide epitope and the specificity of the binding are governed by the heterodimeric αβ T-cell receptors ( 2 ). (frontiersin.org)
  • This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • They show high binding affinity, low toxicity, and are easy to synthesize, all of which makes peptides ideal for use in drug development, and many naturally occurring peptides such as insulin, oxytocin, somatostatin and the antibiotics vancomycin or polymyxin B, are already successfully used like that. (epfl.ch)
  • After screening the libraries, the researchers were able to isolate high-affinity binders to important protein targets. (epfl.ch)
  • The peptides that exhibited the highest affinity for α 3 β 2 -nAChR were used as models for a small-molecule structure similarity search of the PubChem Compound database. (biomedcentral.com)
  • Wilson, D. S., Keefe, A. D. & Szostak, J. W. The use of mRNA display to select high-affinity protein-binding peptides. (nature.com)
  • An affinity based selection technique (biopanning) is used to identify binders from the library. (cdc.gov)
  • Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
  • In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
  • Phage display is used to identify protein or peptide binders to a wide variety of targets. (cdc.gov)
  • SPIDR was tested using the potent and selective 16-amino acid peptide α -conotoxin MII ligand and the α 3 β 2 -nicotinic acetylcholine receptor (nAChR) isoform. (biomedcentral.com)
  • SPIDR incorporates a genetic algorithm-based, heuristic search procedure, which was used to explore the ligand binding domain of the α 3 β 2 -nAChR isoform using a library consisting of 640,000 α -conotoxin MII peptide analogs. (biomedcentral.com)
  • Peptide binding of the ligand takes place in an elongated surface groove as an anti-parallel beta-strand interacts with the beta-B strand and the B helix. (embl.de)
  • When this decoy receptor is engaged by a ligand to the displayed protein/peptide, the receptor expressing cell is rescued from apoptosis. (lu.se)
  • Synthetic peptide libraries are synthesized without utilizing phage or other biological systems. (wikipedia.org)
  • This positional scanning synthetic peptide combinatorial library (PS-SPCL) has been used in rapid identification of serine/threonine kinase phosphorylation motif. (anaspec.com)
  • 2006). The LLNA is based upon characterization of chemicals comprising allergens of different potencies and non- induced proliferative responses in draining lymph nodes allergenic chemicals were evaluated for their ability to react with following topical exposure of mice to chemicals (Gerberick reduced glutathione (GSH) or with two synthetic peptides et al. (cdc.gov)
  • Tedopi is composed of synthetic tumoral neo-epitopes (peptide fragments) that target five tumoral antigens, permitting the activation of tumor-specific T-lymphocytes for patients who are HLA-A2 positive. (medscape.com)
  • Therefore, this class of peptide shows great potential to replace conventional antibiotics. (qub.ac.uk)
  • This study unequivocally demonstrated that QUB-1529 exhibits an inhibitory effect on the growth of Gram-positive bacteria and shows the medical development potential of these peptides in replacing antibiotics to treat Gram-positive bacterial infections. (qub.ac.uk)
  • The results are expected to constitute a significant step towards deciphering the structure-function relationship of IDPs, which is vital for developing the next generations of pharmaceuticals, such as antibiotics, i.e., antimicrobial peptides. (lu.se)
  • The building blocks, which carry only one peptide, do not self-associate, while sufficiently large macrocycles, which display multiple peptides, will self-assemble into extended tubular structures. (europa.eu)
  • Biochemical, biophysical and biological assays using isolated amyloid β peptide, primary neurons and various established Alzheimer's disease nematode models showed that the selected macrocycles potently inhibit the formation of neurotoxic amyloid β peptide aggregates. (nature.com)
  • Partial proteolysis by V8 protease at a single peptide bond immediately N-terminal to the PDZ domain resulted in two distinct and relatively stable fragments and complete loss of catalytic activity. (embl.de)
  • Antimicrobial peptides(AMPs) are short peptide ranging from 10 to 50 amino acids and has a broad spectrum of antibiotic activities against bacteria, yeasts, fungi, and viruses, as well as cytotoxic activity on cancer cells, anti-inflammatory, and immunomodulatory activities. (genscript.com)
  • Therefore, scientists study antimicrobial peptides (AMPs). (uni-marburg.de)
  • The molecule, known as VIRIP (virus-inhibitory peptide), binds to a spiky protein on the surface of the HIV virus called gp41. (bioedonline.org)
  • The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
  • Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)
  • Several covalent bonds, called peptide bonds , join amino acids together to form proteins . (peptidesguide.com)
  • In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
  • Additionally, the antibodies have been characterized for their binding and cross-reactivity properties to several other NPs, and some challenges associated with the isolation of the antibodies from a large library and alternative method for selection of antibodies have been discussed. (cdc.gov)
  • Due to the wide range of binding targets, their large interaction surfaces and biophysical properties, LRR proteins have been suggested as alternatives to antibodies as scaffolds for protein binding. (lu.se)
  • Cyclization of peptides with two chemical bridges affords large scaffold diversities. (epfl.ch)
  • Additionally, predicted CCS values should allow for the use of community libraries, such as the Pan Human library, a repository of over 10,000 human proteins, for targeted proteomics. (news-medical.net)
  • They screened a massive library of compounds isolated from 10,000 litres of filtered human blood, looking for substances that could naturally inhibit HIV. (bioedonline.org)
  • High-throughput capacity: More than 10,000 peptides/month. (novoprolabs.com)
  • Having the library of different peptides at residue 2 and 3 would let the investigator see if some change in chemical properties in the N-terminal tail of the ligated protein makes the protein more useful or useful in a different way. (wikipedia.org)
  • The solution structure of the complex between alpha-bungarotoxin (alpha-BTX) and a 13-residue library-derived peptide (MRYYESSLKSYPD) has been solved using two-dimensional proton-NMR spectroscopy. (rcsb.org)
  • Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
  • Thus, it is possible to develop a library of 20 different polypeptides along with just one amino acid residue randomly, with all the rest being the same. (peptidesguide.com)
  • One subset of the G-protein coupled receptor (GPCR) superfamily is that which is activated by a peptide carrying an obligatory positively charged residue (GPCR-PA+). (sbir.gov)
  • The unique screening technique as described above uses peptide chemistry along with the OBOC library format. (peptidesguide.com)
  • Glu-5 and Leu-8, which also remarkably contribute to the contact area with the toxin, are present in all the library-derived peptides that bind strongly to alpha-BTX. (rcsb.org)
  • Although both peptides bind to similar sites on alpha-BTX, the latter adopts an extended conformation when bound to the toxin [Basus, V., Song, G. & Hawrot, E. (1993) Biochemistry 32, 12290-12298], whereas the library peptide is nearly globular and occupies a larger surface area of alpha-BTX binding site. (rcsb.org)
  • We employ new Natural Language Processing (NLP) based methods to predict whether any TCR and peptide bind. (frontiersin.org)
  • A set of standard tests are defined for the performance of peptide-TCR binding, including the detection of TCRs binding to a given peptide/antigen, choosing among a set of candidate peptides for a given TCR and determining whether any pair of TCR-peptide bind. (frontiersin.org)
  • EPFL scientists have developed a new type of "double-bridged peptide" that can be tailored to bind tightly to disease targets of interest. (epfl.ch)
  • Peptides are short chains of amino acids that can bind to proteins and change their function. (epfl.ch)
  • Using the double-bridge approach for both these protein targets, the scientists developed peptides that could bind them efficiently at nanomolar concentrations. (epfl.ch)
  • In the case of kallikrein, the peptides could bind it for more than an hour before dissociating. (epfl.ch)
  • Understanding the assembly behavior of peptides is important in not only designing nanomaterials for a desired functionality but also for combating neurodegenerative diseases such as Alzheimer and Parkinson's disease which are strongly associated with an accumulation of amyloid forming peptides in the brain. (lu.se)
  • Allocation of the library and a test cell line within a soft agar matrix followed by a partial peptide release will result in high concentrations of peptide in the agar surrounding each bead. (peptidesguide.com)
  • Increasing the number of hydrophobic alanines to A8K and A10K, aggregation begins at lower peptide concentrations. (lu.se)
  • The bound peptide adopts an almost-globular conformation resulting from three turns that surround a hydrophobic core formed by Tyr-11 of the peptide. (rcsb.org)
  • They found that when the size of the hydrophilic dendron becomes too large, it limits 1D growth, producing objects that are restricted in size and globular in shape. (europa.eu)
  • By producing large numbers of peptides, for which each differs from the other by one single amino acid, it is possible to ascertain which amino acid substitutions make binding to a receptor the strongest, and which make that binding process weaker. (peptidesguide.com)
  • Prearranged positive allosteric modulators (PAMs) inspired by photopharmacology are synthesized and applied to the glucagon-like peptide-1 receptor (GLP-1R), a class B G protein-coupled receptor. (wiley.com)
  • We have demonstrated proof of concept by creating a general plasma membrane bound decoy receptor, by displaying a protein or a peptide genetically fused to a trunctated version of the CD40 molecule. (lu.se)
  • One specific peptide-displaying cell could be identified and amplified, based on a specific receptor engagement, in a background of 12 500 wild-type cells after four selections. (lu.se)
  • Topical antibiotic was applied onto the how to dissolve peptide edges of the wound to prevent subsequent dermal infection. (alksignaling.com)
  • The other is to use a set of the patient's own personalized peptides that harbor cancer mutations as vaccines. (utexas.edu)
  • Release of substance P and the effects of specific antagonists would be used to determine the synaptic coupling to down stream cholinergic or vasoactive intestinal peptide-immunoreactive neurons. (usda.gov)
  • To address this problem, we used an analysis platform that coupled hexapeptide libraries for dynamic range compression (DRC) with three-dimensional (3D) peptide fractionation. (umn.edu)
  • Compared to saliva not treated with hexapeptide libraries, DRC substantially increased identified proteins across physicochemical and functional categories. (umn.edu)
  • The Nature Communications paper describes CCS values measured on the timsTOF Pro as an essentially intrinsic property of the peptide ions, which can be used to improve confidence in peptide and protein group identification in 4D shotgun proteomics. (news-medical.net)
  • β-sheet breaker peptides inhibit fibrillogenesis in a rat brain model of amyloidosis: implications for Alzheimer's therapy. (nature.com)
  • Following specific binding of T cell receptors to viral and bacterial-derived peptides bound to MHC ( 5 ), or from neo-antigens ( 8 - 10 ), the appropriate T cells expand, resulting in the increased frequency of T cells carrying such receptors. (frontiersin.org)
  • The double-bridged peptides are hardly degraded by proteases in the blood, which is a great advantage because it keeps the peptides from being eliminated too quickly, thus extending their therapeutic effects. (epfl.ch)
  • Successful recognition of antigenic peptides bound to Major Histocompatibility Complexes (pMHCs) requires specific binding of the TCR to these complexes ( 5 - 7 ), which in turn modulates the cell's fitness, clonal expansion, and acquisition of effector properties ( 7 ). (frontiersin.org)
  • Large, noncollagenous glycoprotein with antigenic properties. (bvsalud.org)
  • However, using peptides as drugs faces two challenges. (epfl.ch)
  • Examples include chemical compounds including drugs, nucleotides including siRNAs and miRNAs, carbohydrates, lipids, peptides and chemically-modified macromolecules. (mcmaster.ca)
  • SPIDR identified non-peptide drugs with estimated binding affinities nearly double that of the native α -conotoxin MII peptide. (biomedcentral.com)
  • Developing new techniques for screening and synthesizing peptides can result in efficient and cost-effective methods which make drugs more effective, diseases easier to cure, and lives to last longer. (peptidesguide.com)
  • There is an increasing demand for peptide libraries from pharmaceutical companies due to the expanding interest in peptide drugs. (novoprolabs.com)
  • Lerner, R. A. & Brenner, S. DNA-encoded compound libraries as open source: a powerful pathway to new drugs. (nature.com)
  • Kirchhoff's team had access to a large library of blood compounds at IPF PharmaCeuticals, a pharmaceutical company in Germany keen to develop the work. (bioedonline.org)
  • So peptide libraries are very capable tools for biological and pharmaceutical research. (peptidesguide.com)
  • Currents tools are based on conserved motifs and are applied to peptides with many known binding TCRs. (frontiersin.org)
  • From this number of total combinations, the scope of the peptide library can be narrowed down for a more specific purpose by selecting which amino acids are desired at each point in the chain. (wikipedia.org)
  • c) Frequency of peptide C-terminal amino acids. (news-medical.net)
  • Due to the negative charge of many microbial membrane components (e.g. phospholipids, lipopolysaccharides and teichonic acids), antimicrobial peptides are often cationic in nature. (genscript.com)
  • Second, peptide stability: peptides can potentially be cut by enzymes (proteases) into smaller and useless fragments or even single amino acids. (epfl.ch)
  • In addition, the chemists systematically changed the amino acids between the cysteines and generated libraries of billions of different double-bridged peptides. (epfl.ch)
  • For several reasons there is a growing interest in the scientific community in immunoglobulin Y as antibiotic alternative and their oral administration in the polyclonal antibody (pAb) format, to maintain animal health and performance, do not require IgY purification for large-scale production, resulting in protein impurities and high concentration of biotin in the samples. (preprints.org)
  • Based on the results, Heinis's lab is now applying the peptide format to many other disease targets. (epfl.ch)
  • They have already developed new, even larger double-bridged peptide libraries and screened them against a range of disease-relevant targets. (epfl.ch)
  • In this Perspective, we discuss the recent progress in using DNA-encoded chemical libraries to interrogate complex biological targets and their potential to identify structures that elicit function or possess other useful properties. (nature.com)
  • Usually, the peptide library is synthesized on a solid phase, mostly on resin, which can be made as a flat surface or beads. (wikipedia.org)
  • In an off-bead assay, the peptide is released from the resin bead, but it maintains its proximity to the bead. (peptidesguide.com)
  • In contrast, many TCR responses are characterized by a high level of cross-reactivity with single TCRs binding a large number of MHC-bound peptides, and single peptides binding a large number of TCRs ( 16 , 17 ). (frontiersin.org)
  • In this study, a molecular cloning strategy was used to clone the peptide encoding cDNA from the frog skin secretion cDNA library, and then the pure peptide was obtained by using RP-HPLC and MALDI-TOF MS techniques. (qub.ac.uk)
  • Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. (wiley.com)
  • DNA-encoded chemical libraries (DELs) have emerged as a powerful technology in drug discovery. (nature.com)
  • Neri, D. & Lerner, R. A. DNA-encoded chemical libraries: a selection system based on endowing organic compounds with amplifiable information. (nature.com)
  • A key roadblock to success has been the question of how large volume chemical libraries can be efficiently screened for active compounds. (oregonstate.edu)
  • To mitigate these technical challenges, an algorithm-supported approach to peptide library design may use molecular mass and amino acid diversity to simplify the laborious permutation identification in complex mixtures when using mass spectrometry. (wikipedia.org)