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  • quantitation
  • The advantage of metabolic labeling over in vitro tagging techniques is that the heavy and unlabeled samples are mixed before sample preparation, preventing variability between preparations from distorting the final quantitation results. (isotope.com)
  • transitions
  • In the actual area ratio calculation, the summed areas of all MRM transitions for each peptide were used. (nih.gov)
  • 2011 ). MRM experiments are typically conducted using triple quadrupole mass spectrometers to scan specific ion pairs of mass-to-charge ( m / z ) values associated with the peptide precursor and fragment ions, which are referred to as transitions. (springer.com)
  • Purity
  • These state-of-the-art synthesizers, coupled with our experienced peptide chemists, enable us to deliver our peptides quickly and in high quality (purity of 90-95% is typical). (mobitec.com)
  • trypsin
  • The membranes were trypsin digested and the peptides were analyzed using LC-MS/MS under optimized conditions. (hindawi.com)
  • enrichment
  • For example, in peptide-centric workflows that target post-translationally modified (PTM) peptides, antibody or other enrichment steps may be incompatible with the chemical tags. (mcponline.org)
  • Our own effort to combine an anti-acetyllysine antibody enrichment step with iTRAQ was largely unsuccessful, with the enrichment efficiency of peptides containing this modification significantly lower than without chemical labeling. (mcponline.org)
  • acetylation
  • To demonstrate the utility of the MS1 filtering approach, we have carried out experiments on several MS platforms and have specifically examined the performance of this method to quantify two important post-translational modifications: acetylation and phosphorylation, in peptide-centric affinity workflows of increasing complexity using mouse and human models. (mcponline.org)
  • workflows
  • It is based on linear mixed-effects models and is applicable to most experimental designs for both isotope label-based and label-free SRM workflows. (mcponline.org)
  • biological
  • Originally developed almost 100 years ago to measure elemental atomic weights and the natural abundance of specific isotopes, MS was first used in the biological sciences to trace heavy isotopes through biological systems. (thermofisher.com)
  • target
  • The biotin end of the peptide is bound to streptavidin, and the other end is recognized by a target specific antibody. (mobitec.com)