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  • MALDI
  • The most widely used instrument for peptide mass analysis are the MALDI-TOF instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. (wikipedia.org)
  • In matrix-assisted laser desorption ionization (MALDI), a fragmented peptide sample is loaded onto a matrix and ionized through the use of a high energy laser. (wikipedia.org)
  • citation needed] A small fraction of the peptide (usually 1 microliter or less) is pipetted onto a MALDI target and a chemical called a matrix is added to the peptide mix. (wikipedia.org)
  • Matrix and peptide molecules co-crystallize on the MALDI target and are ready to be analyzed. (wikipedia.org)
  • murine
  • Zhang Y, McCormick L, Gilliam A. Latency-associated peptide prevents skin fibrosis in murine sclerodermatous graft-versus-host disease, a model for human scleroderma. (labome.org)
  • Short peptide fragments of human and murine interleukin 1 (IL 1) were synthesized on the basis of their predicted exposure on the surface of the molecule in an attempt to identify the minimal structure responsible for the immunostimulatory activity of IL 1. (semanticscholar.org)
  • 1993
  • 1993, Tetrahedron Letters 49:9307-9320) no techniques currently exist which can be utilized for large scale, economical production of easily purified peptides such as T-1249. (allindianpatents.com)
  • hydrophobic
  • These shorter A β fragments are also easier to synthesize and more cost-effective than the highly hydrophobic full-length A β 1-42 peptide. (ingentaconnect.com)
  • The adsorption on hydrophobic and hydrophilic silica-based surfaces of the integrin-binding PHSRN peptide and the single-residual-mutated analogues, PHSEN and PHSFN, is investigated by comparative QCM-D, XPS, SFG measurements and molecular dynamics calculations. (chalmers.se)
  • Since the peptide bonds themselves are polar they are neutralised by hydrogen bonding with each other when in the hydrophobic environment. (wikipedia.org)
  • Another characteristic that must be pointed out is that p3 peptides have more solvent-exposed hydrophobic surfaces (60%) than Aβ oligomers do (20%), so buried surface areas are not as big within p3 oligomers (30%) as they are within Aβ oligomers. (wikipedia.org)
  • Although p3 peptide can assemble into fibrillar aggregates, its hydrophobic properties make it unable to rest in oligomeric forms. (wikipedia.org)
  • cleavage
  • Each purified peptide was individually incubated with recombinant MT1-MMP or MT3-MMP in the presence or absence of Cu 2+ and the cleavage sites determined by LC-ESI-MS analysis. (mdpi.com)
  • The fragmentation process primarily gives rise to cleavage products that break along peptide bonds. (wikipedia.org)
  • affinity
  • This high affinity increases the probability of CD8+ t-cell recognition of EBV peptides held by HLA-A*02 complexes. (wikipedia.org)
  • Since each HLA has a different affinity for peptides of certain structures, greater variety of HLAs means greater variety of antigens to be 'presented' on the cell surface, enhancing the likelihood that a subset of the population will be resistant to a given foreign invader. (wikipedia.org)
  • molecules
  • The matrix molecules are required for the desorption of the peptide molecules. (wikipedia.org)
  • The energy transfer is sufficient to promote the ionisation and transition of matrix molecules and peptides from the solid phase into the gas phase. (wikipedia.org)
  • The peptide-loading process involves several other molecules that form a large multimeric complex consisting of TAP, tapasin, calreticulin, calnexin, and Erp57 (PDIA3). (wikipedia.org)
  • invention
  • This invention concerns peptide fragments of IFPS which are highly active against P. aeruginosa and other Pseudomonas species. (innovations-report.com)
  • The present invention relates to a method for synthesizing a peptides of peptides referred to as T-1249 and T-1249-like peptides. (allindianpatents.com)
  • Generally, the methods of the invention comprise synthesizing specific side-chain protected peptide fragment intermediates of T-1249 or a T-1249-like peptide on a solid support, coupling the protected fragments in solution to form a protected T-1249 or T-1249-like peptide, followed by deprotection of the side chains tc yield the final T-1249 or T-1249-like peptide. (allindianpatents.com)
  • The groups of peptide fragments according to the invention include Groups 1-6, as designated in Table 2 below. (allindianpatents.com)
  • This invention is based, in part, on the inventors' unexpected discovery that certain, combinations of solid phase liquid phase methetic reactiorns allow high p-urity T-1249 and T-1249-like peptides to be manufactured for the first time on a large scale with high throughput and high yield. (allindianpatents.com)
  • Functional
  • Chromogranin A is the precursor to several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. (wikipedia.org)
  • bonds
  • Several fragments of CART have been tested to try and uncover the pharmacophore, but the natural splicing products CART 55-102 and CART 62-102 are still of highest activity, with the reduced activity of smaller fragments thought to indicate that a compact structure retaining all three of CART's disulphide bonds is preferred. (wikipedia.org)
  • widely
  • CART is an anorectic peptide and is widely expressed in both the central and peripheral nervous systems, particularly concentrated in the hypothalamus. (wikipedia.org)
  • fibrils
  • Scaffolded multimers of hIAPP(20-29) peptide fragments fibrillate faster and lead to different fibrils compared to the free hIAPP(20-29) peptide fragment. (sigmaaldrich.com)
  • Applying fibril-forming peptides in nanomaterial design is still challenged by the difficulties in understanding and controlling how fibrils form. (sigmaaldrich.com)
  • ThT fluorescence, CD and FTIR spectroscopy, and AFM and TEM imaging reveal that in both conjugates the peptide retained its fibrillating properties and formed fibrils. (sigmaaldrich.com)
  • However, the conjugate fibrils formed more rapidly than the free peptide and were long and thin, as opposed to the thick and twisted morphology of the intact peptide. (sigmaaldrich.com)
  • As a consequence, p3 peptide structural determinants can assemble into fibrils, but no oligomeric forms have been identified. (wikipedia.org)
  • respectively
  • Thus any alteration to the HLA that induces decreased binding to a certain peptide or increased binding to a certain peptide, is expressed as, respectively, increased susceptibility to disease or decreased susceptibility to disease. (wikipedia.org)
  • stable
  • Having these facts into account, we can state that p3 oligomers' existence is thermodynamically unfavourable, which suggests that the p3 peptide cannot form stable soluble oligomers in the same way Aβ does. (wikipedia.org)
  • membrane
  • SYTOX green was applied to evaluate the effects of the peptides on the membrane integrity of the bacterial cells. (up.ac.za)
  • LL-37, a peptide known to disrupt microbial membranes, induced membrane permeabilization of both E. coli and S. aureus membranes. (up.ac.za)
  • The MHC I:peptide complex is then inserted via endoplasmic reticulum into the external plasma membrane of the cell. (wikipedia.org)
  • They have membrane proximal Ig fold The peptide translocation from the cytosol into the lumen of the ER is accomplished by the transporter associated with antigen processing (TAP). (wikipedia.org)
  • genome
  • This method is an improvement from previous methods because it compares the peptide fingerprints to an entire genome instead of comparing it to an already annotated genome. (wikipedia.org)
  • This search against the whole genome revealed that approximately 4% of unique peptide that they found were outside of previously annotated regions. (wikipedia.org)
  • found
  • The amidated peptide was found to be non-toxic towards human erythrocytes and Caco-2 cells. (up.ac.za)
  • The peptide is found in several areas, among them the ventral tegmental area (VTA) of the brain. (wikipedia.org)
  • different
  • Attachment of the peptide to the templates resulted in multimers containing six peptide fragments at different positions. (sigmaaldrich.com)
  • The aim of the present study was to investigate the potential retinoprotective effects of different PACAP fragments (PACAP 4-13, 4-22, 6-10, 6-15, 11-15, and 20-31) and related peptides (secretin, glucagon) in BCCAO-induced ischemic retinopathy. (hindawi.com)
  • mass
  • The sections were analyzed with TOF-secondary ion mass spectrometry, and the peptides formed were identified through comparison with the peptides of the thyroglobulin reference sample. (chalmers.se)
  • One major advantage to mass fingerprinting is that it is significantly faster to carry out than peptide sequencing, yet the results are equally useful. (wikipedia.org)
  • The peptides are then dissolved in a small amount of distilled water or further concentrated and purified using ZipTip Pipette tips and are ready for mass spectrometric analysis. (wikipedia.org)
  • results
  • Our results suggest that the safety and efficacy issues previously encountered in other A β vaccination trials may be successfully addressed by using micelle-encapsulated peptides. (ingentaconnect.com)
  • These results are consistent with alternate MHC-I Ag processing mechanisms that involve binding of peptides to MHC-I within acidic vacuolar compartments. (labome.org)
  • All hgh peptide fragment 176 191 results wholesalers & hgh peptide fragment 176 191 results manufacturers come from members. (lightneasy.org)
  • We doesn't provide hgh peptide fragment 176 191 results products or service, please contact them directly and verify their companies info carefully. (lightneasy.org)
  • Our results revealed significant neuroprotection by PACAP 1-38 but did not reveal retinoprotective effect of the PACAP fragments or related peptides. (hindawi.com)
  • method
  • 2. The method according to claim 1, wherein the foreign substance is any organic compound selected from a group consisting of peptides, nucleic acids, dyes, and drugs. (patentsencyclopedia.com)
  • increases
  • Although still less potent than Os, the determined minimum bactericidal concentration (MBC) for each peptide indicated that amidation increases the bactericidal activity of Os(3-12) by 16-fold against Escherichia coli and by 8-fold against both Pseudomonas aeruginosa and Bacillus subtilis. (up.ac.za)
  • Several
  • CART peptides, in particular, CART (55-102), seem to have an important function in the regulation of energy homeostasis, and interact with several hypothalamic appetite circuits. (wikipedia.org)
  • CART expression is regulated by several peripheral peptide hormones involved in appetite regulation, including leptin, cholecystokinin and ghrelin, with CART and cholecystokinin having synergistic effects on appetite regulation. (wikipedia.org)
  • cell
  • Niland B, Banki K, Biddison W, Perl A. CD8+ T cell-mediated HLA-A*0201-restricted cytotoxicity to transaldolase peptide 168-176 in patients with multiple sclerosis. (labome.org)
  • Endothelial cell cultures on the peptide-functionalized materials highlight their tunable pro- or anti-angiogenic potential. (chalmers.se)
  • The α3-CD8 interaction holds the MHC I molecule in place while the T cell receptor (TCR) on the surface of the cytotoxic T cell binds its α1-α2 heterodimer ligand, and checks the coupled peptide for antigenicity. (wikipedia.org)