Loading...
  • translation elongation factor
  • MAC was tested on a practical dataset, namely, two non-coding fragments of the translation elongation factor 1 alpha gene from Chinese hamster ovary cells.The individual fragment lengths exceeded 5 kbp, and direct PCR amplification produced no amplicons.This approach may be used to generate long artificial DNA fragments such as in vitro spliced cDNAs. (nih.gov)
  • MAC was tested on a practical dataset, namely, two non-coding fragments of the translation elongation factor 1 alpha gene from Chinese hamster ovary cells. (nih.gov)
  • structural
  • In order to understand this process and to determine the exact conformation of the co-translational folding intermediates, it is essential to develop techniques that allow the isolation of RNCs carrying nascent chains of predetermined sizes to allow their further structural analysis. (jove.com)
  • Regulation
  • Importantly, combining polysome profiling with high throughput genomic approaches allows for a large scale analysis of translational regulation. (jove.com)
  • Enzymatic
  • Enzymatic resynthesis of the hydrolyzed peptide bond(s) in ribonuclease S. (naver.com)
  • Several mutants of the GCN4 leucine zipper are catalytically inactive, providing important negative controls and unequivocally associating the enzymatic activity with the peptide under study. (jove.com)
  • Leucine
  • As an additional result of this work, the retro-leucine zipper of GCN4 can be added to the list of functional retro-peptides. (jove.com)
  • increase
  • Our data support the hypothesis that the possible elimination of translational pauses that increase translation rate leads to protein misfolding and aggregation. (biomedcentral.com)
  • High concentrations of circulating P(4) in the immediate post-conception period have been associated with an advancement of conceptus elongation, an associated increase in interferon-tau production and higher pregnancy rates in cattle. (semanticscholar.org)
  • control
  • We investigated the role of translational control in cyclically (0.5 Hz, 0.1 Hz and 0.05 Hz) and statically loaded porcine articular cartilage explants. (ox.ac.uk)