• Medium-chain fatty acids are molecules with applications in different industries and with growing demand. (biomedcentral.com)
  • The reverse β-oxidation pathway is an energy-efficient pathway that produces medium-chain fatty acids in microorganisms, and its use in Saccharomyces cerevisiae , a broadly used industrial microorganism, is desired. (biomedcentral.com)
  • However, the application of this pathway in this organism has so far either led to low titers or to the predominant production of short-chain fatty acids. (biomedcentral.com)
  • We genetically engineered Saccharomyces cerevisiae to produce the medium-chain fatty acids hexanoic and octanoic acid using novel variants of the reverse β-oxidation pathway. (biomedcentral.com)
  • Medium-chain fatty acids (MCFAs) are six to ten hydrocarbon chain long carboxylic acids with applications in a broad range of sectors. (biomedcentral.com)
  • Currently, MCFAs are either produced via non-sustainable petrochemical routes or extracted directly from palm or coconut tree seeds, representing up to 5 and 10% of the fatty acid content, respectively [ 43 , 61 , 65 ]. (biomedcentral.com)