• In order to find the effects of these fruits, neuron like PC12 cells were exposed to the extracts of these fruits before H(2)O(2) treatment. (nih.gov)
  • Exploiting this capacity of SCBD, we challenged mechanosensing/-transduction and differentiative behavior of neuron-like PC12 cells with diverse nanotopographies and/or changes of their biomechanical status, and analyzed their phosphoproteomic profiles in these settings. (frontiersin.org)
  • However, pretreatment with the root extract of R. turkestanicum significantly reduced MDA content, ROS generation and apoptotic cell death. (termedia.pl)
  • However, more recently it has become clear that cellular Ca2+ overload, or perturbation of intracellular Ca2+ compartmentalization, can cause cytotoxicity and trigger either apoptotic or necrotic cell death. (scienceopen.com)
  • Since treatment with I μM MPP + caused apoptotic cell death in p21(WAF1/Cip1)-deficient (p21(-/-)) but not in parental (p21(+/+)) mouse embryo fibroblasts, our data suggest that in an early phase MPP + -induced p21(WAf1/Cip1) expression leads to growth arrest and prevents apoptosis until energy depletion finally leads to a nonapoptotic cell death. (elsevier.com)
  • The number of apoptotic cells was assessed using annexin V-FITC/PI apoptosis detection kit. (1library.net)
  • Apoptotic cell death also occurred. (uniss.it)
  • he intracellular distribution of 125I-labeled nerve growth factor (NGF) in rat pheochromocytoma PC12 cells was studied by quantitative electron microscopic (EM) autoradiography and by subcellular fractionation. (jneurosci.org)
  • Pre-treating cells with butyl ferulate (FAC4) significantly attenuated Aβ 1-42 -evoked intracellular ROS formation. (rsc.org)
  • Real-time intracellular calcium level recordings were performed on PC-12 cell suspensions, and ET-1 induced a dose-dependent decrease in the K + -evoked calcium levels. (aspetjournals.org)
  • To expound the protective mechanism, we investigated the effects of ghrelin on high glucose-induced cell apoptosis and intracellular signaling in cultured PC12, which is a suitable model for studying neuronal cell death. (springer.com)
  • The actions of rikkunshito on PC12 cells were evaluated by measuring intracellular cAMP levels, tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP) mRNA expression levels, and catecholamine levels in the culture medium. (biomedcentral.com)
  • Membrane composition serves to identify intracellular compartments, signal cell death, as well as to alter a cell's electrical and physical properties. (chalmers.se)
  • Increasing intracellular Cu with a cell-permeable Cu-complex (CuII(gtsm)) resulted in increased expression of synaptophysin and robust translocation of this and additional vesicular proteins from synaptic-like microvesicle (SLMV) fractions to chromogranin-containing putative large dense core vesicle (LDCV) fractions in density gradient preparations. (monash.edu)
  • These data suggest that calmodulin mediates ERK activation induced by increases in intracellular Ca2+ concentration in PC12 cells by a mechanism that seems to be independent of Trk A and EGFR activation. (udl.cat)
  • Overexpression of wild type Ngb, but not of mutant Ngb, significantly attenuated H2O2-induced ROS/RNS accumulation and lipid peroxidation, decreased H2O2-induced mitochondrial dysfunction and apoptosis, and promoted overall cell survival. (nih.gov)
  • Using PC12 cell model, we showed that Glu and Hcy provoked a huge amount of reactive oxygen species (ROS) production, causing mitochondrial damage at EC 50 20 and 10 mm, respectively. (mdpi.com)
  • Replacement by chloride of glutamate, which could sustain mitochondrial ATP production in permeabilized cells, does not significantly alter catecholamine release induced by Ca2+. (uni-muenchen.de)
  • Wüllner, U. 1999-04-02 00:00:00 We investigated the effect of glutathione (GSH) depletion on mitochondrial function and generation of reactive oxygen intermediates (ROI) in PC12 cells in vitro. (deepdyve.com)
  • In contrast, BSO-treated cells still maintained 100% GSH in the mitochondrial fraction for 4 h and 6% for 48 h. (deepdyve.com)
  • Inhibition of any of the ETC complexes, ATP synthase or dissipation of the mitochondrial membrane potential was sufficient to completely abolish mitochondrial fusion in differentiated PC12 cells. (tcd.ie)
  • Accumulations of this protein have been shown to inhibit complex I. Overexpression of both wild-type and mutant forms of a-synculein were found to decrease mitochondrial fusion in differentiated PC12 cells, suggesting that a-synculein dependent changes in fusion/fission dynamics may be a complicating factor in PD. (tcd.ie)
  • In this study, inhibition of fatty acid oxidation and pyruvate transport into the mitochondrion decreased mitochondrial fusion in differentiated PC12 cells. (tcd.ie)
  • A decrease in the level of O-GlcNAcylation, induced by inhibition or knockdown of O-GlcNAc transferase, resulted in increased mitochondrial fusion, decreased mitochondrial membrane potential, fragmented mitochondrial morphology and decreased respiration rates in differentiated PC12 cells. (tcd.ie)
  • Background: T-type Ca2+ (Cav3.2) channel inhibitors prevent neuronal death during ischemia, but the cytotoxic signals have not been resolved.Results: Cav3.2 channels generate cytotoxic Ca2+ elevations in the cytosol and mitochondria of PC12 cells deprived of oxygen and glucose.Conclusion: Ca2+ transfer from Cav3.2 channels to mitochondria contributes to ischemic toxicity.Significance: Cav3.2 channels and the mitochondrial Ca2+ uniporter (MCU) are potential targets for the treatment of stroke. (scienceopen.com)
  • Mitochondrial Ca2+ homeostasis plays a key role in the regulation of aerobic metabolism and cell survival 1 , but the molecular identity of the Ca2+ channel, the mitochondrial calcium uniporter 2 , was still unknown. (scienceopen.com)
  • siRNA silencing of MCU in HeLa cells drastically reduced mitochondrial Ca2+ uptake. (scienceopen.com)
  • The ATP pool in differentiated cells was much more labile than that of undifferentiated cells in the presence of agents that dissipated mitochondrial proton gradients. (nih.gov)
  • The inhibition of cell growth was not dependent on an inhibition of complex I activity since MPP + also inhibited cell proliferation in SH-SY5Y cells lacking mitochondrial DNA and complex I activity (p 0 cells). (elsevier.com)
  • Silibinin A (SIL A) is one of two diastereomers found in silymarin and was used to evaluate the effects of silymarin on mitochondrial parameters including mitochondrial membrane potential and ATP production with and without sodium nitroprusside- (SNP-) induced nitrosative stress, oxidative phosphorylation, and citrate synthase activity in HepG2 and PC12 cells. (hindawi.com)
  • It was also proposed that SIL A shows an effect on the permeability of mitochondrial membranes in liver cells, affecting the integration of cholesterol into the lipid bilayer [ 2 ]. (hindawi.com)
  • The cells were treated with various concentrations of cocaine and they observed that general mitochondrial activity was not bothered. (bergbuilds.domains)
  • However, when they measured the mitochondrial membrane potential using fluorescence probe Rh 123, the data indicated that cocaine treatment significantly decreased the mitochondrial membrane potential in cells compared to the control. (bergbuilds.domains)
  • When there is disruption in the mitochondrial potential, the cell switches to anaerobic glycolysis to survive. (bergbuilds.domains)
  • Mitochondrial potentials were higher in 123.7 cells than in WT cells, indicating that PKA inhibits mitochondrial metabolism. (louisville.edu)
  • The greater expression of COX IV may allow 123.7 cells to maintain mitochondrial electron flow and energy production under drastically reduced oxygen conditions. (louisville.edu)
  • abstract = "PC12 cells are spherical when cultured on plastic, but flatten and spread extensively when cultured on extracellular matrix (ECM) produced by bovine corneal endothelial cells. (elsevier.com)
  • abstract = "PC12 cells are an established model for studying the role of N-methyl-d-aspartate (NMDA) receptors in excitotoxicity and function as multimeric assemblies of NR1 with at least one NR2(A-D) subunit. (edu.au)
  • abstract = "Copper (Cu) is an essential biometal involved in a number of cell functions. (monash.edu)
  • abstract = "The effects of different levels of inorganic mercury (Hg2+) on depolarization- or agonist-stimulated Ca2+ signals were studied in PC12 cells. (elsevier.com)
  • Published March 15, 2017 Abstract: This study aimed to investigate the effects of miR-29a-3p on the injury of PC-12 cells induced by H2O2. (1library.net)
  • It was found that PC12 cells persist and continue to proliferate within the brain of SD rats whereas it has previously been reported that grafts placed into peripheral sites do not proliferate. (deepdyve.com)
  • However, since NCS-1 and DARPP-32 levels are not altered in wild type rats, we treated wild type PC12 cells (PC12 WT) and PC12 cells stably overexpressing NCS-1 (PC12 Clone) with antipsychotics to investigate if NCS-1 upregulation modulates DARPP-32 expression in response to antipsychotics treatment. (biomedcentral.com)
  • This research project identified some mechanisms of Brn3b-mediated neuroprotection in cultured PC12 cells (under the condition of hypoxia) and also in vivo in the Morrison's model of ocular hypertension in rats. (unt.edu)
  • This study aimed to investigate the effect and the molecular basis of miR-103 on LPS-induced injuries in PC12 cells in vitro and SCI rats in vivo . (ac.ir)
  • We also found that miR-103 agomir inhibited cell apoptosis and autophagy in SCI rats. (ac.ir)
  • This study demonstrates that miR-103 may represent a protective effect against cell apoptosis and autophagy in LPS-injured PC12 cells and SCI rats by upregulation of SOX2 expression. (ac.ir)
  • Cell survival in both untreated and nerve growth factor (NGF)-treated PC12 cells was inhibited by down-regulation of SOD1, with NGF-treated cells dying at lower levels of inhibition than untreated cells. (pnas.org)
  • These results suggest that the induction of cell death by inhibition of SOD1 is due to free radical induction of apoptosis and that growth factor therapy for free-radical-mediated disease may require antioxidants in order to be effective. (pnas.org)
  • Preincubation of PC12 cells with pertussis toxin reversed the ET-1-induced inhibition of the K + -evoked ATP release. (aspetjournals.org)
  • Nongenomic inhibition of catecholamine secretion by 17beta-estradiol in PC12 cells. (semanticscholar.org)
  • These results suggest that (-)-sesamin protects 6-OHDA-induced cytotoxicity via the activation of transient ERK1/2-BadSer112 system and the inhibition of sustained ERK-p38MAPK-JNK1/2-caspase-3 system in PC12 cells. (ovid.com)
  • We used pharmacological inhibition of Ca2+ transporters to characterize Ca2+ clearance after depolarizations in both undifferentiated and nerve growth factor-differentiated PC12 cells. (nih.gov)
  • Expression of the H+ pump, V-ATPase, which is essential for vesicle maturation, was increased in Cu-treated cells while inhibition of V-ATPase prevented translocation of synaptophysin to LDCV fractions. (monash.edu)
  • SOX2 overexpression protected PC12 cells against LPS-induced injuries, while SOX2 inhibition expedited LPS-induced cell injuries. (ac.ir)
  • Their results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydrogen peroxide-induced oxidative stress-mediated injury of PC12 cells. (eurekalert.org)
  • These results suggested that stretch-induced injury of PC12 cells is dependent on strain magnitude and strain rate, but, in order to elucidate neuronal damage caused by an interaction between strain magnitude and strain rate, more quantitative studies are required, such as various combination of strain magnitude and strain rate. (springerprofessional.de)
  • We investigated the effects of 17beta-estradiol, an estrogen, on [(3)H]norepinephrine ([(3)H]NE) secretion in PC12 cells. (semanticscholar.org)
  • Expression of an APP phosphorylation-deficient mutant decreased both basal and constitutive secretion relative to the APP wild-type, suggesting a role for APP-Thr668 phosphorylation in secretion in PC12 cells. (edu.mn)
  • Protective effect of rAAV/ABAD-DP on H2O2-mediated changes in morphology of PC12 cells. (eurekalert.org)
  • We observe the morphology of PC12 cells using the multi photon microscope and evaluate effectiveness of uniform ACMF stimulation of the nerve axonal extension and the neural network generation. (asme.org)
  • SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells," Oxidative Medicine and Cellular Longevity , vol. 2016, Article ID 4202437, 11 pages, 2016. (hindawi.com)
  • In this study, we investigated the neuroprotective effects of Rheum turkestanicum in the glutamate-induced rat pheochromocytoma (PC12 cells) and mouse neuroblastoma (N2a) cell lines. (termedia.pl)
  • All these findings indicated that R. turkestanicum protected PC12 and N2a cells against glutamate-induced oxidative cell death and apoptosis and might raise the possibility of R. turkestanicum usage as a neuroprotective agent. (termedia.pl)
  • PC12 and neuro 2a cells have different susceptibilities to acetylcholinesterase-amyloid complexes, amyloid 25-35 fragment, glutamate, and hydrogen peroxide. (termedia.pl)
  • In neuroendocrine PC12 cells, immature secretory granules (ISGs) mature through homotypic fusion and membrane remodeling. (rupress.org)
  • The properties and localization of synaptophysin were compared between transfected CHO-K1 cells and native neuroendocrine PC12 cells. (elsevier.com)
  • We previously showed, using PC12nnr5 cells transfected with myc-tagged 14-3-3β and η) isoforms, that 14-3-3s form a complex with tyrosine hydroxylase (TH) particularly when the hydroxylase is phosphorylated at Ser-19 by endogeneous Ca 2+ , calmodulin-dependent protein kinase II 1 . (springer.com)
  • Ichimura T., Itagaki C., Wakamiya A., Isobe T. (2002) Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. (springer.com)
  • We compared the role of tyrosine kinases in α 1A -adrenergic receptor (AR) and growth factor receptor stimulation of mitogen-activated protein kinase pathways in PC12 cells. (biochemj.org)
  • Norepinephrine (NE) (noradrenaline), epidermal growth factor (EGF) and nerve growth factor (NGF) caused different patterns of tyrosine phosphorylation in PC12 cells stably expressing α 1A -ARs. (biochemj.org)
  • These studies show that activation of mitogen-activated protein kinase pathways by G-protein-coupled receptors and tyrosine kinase receptors proceed through distinct molecular pathways in PC12 cells, and support an obligatory role for Src activation in mitogenic responses to α 1A -ARs in these cells. (biochemj.org)
  • Dispersed and aggregated PC12 cell grafts contained immunoreactivity to tyrosine hydroxylase and aromatic amino acid decarboxylase, but failed to express phenylethanolamine n-methyltransferase. (deepdyve.com)
  • The Trk tyrosine kinase inhibitor K252a did not block the neuritogenic effects of primary Schwann cells. (ox.ac.uk)
  • Again, in contrast with NGF or SCTM41 cells, the primary Schwann cell effect was Trk tyrosine kinase independent. (ox.ac.uk)
  • In this study, we demonstrate that L-DOPA induces death of catecholaminergic cells in vitro via an active program of apoptosis. (jci.org)
  • This work aims to ascertain the structure-activity relationship of FA and its alkyl esters (FAEs) for evaluating the antioxidant activities in PC12 cells and Aβ 1-42 aggregation inhibitory activities in vitro , as well as the signaling mechanisms against oxidative stress elicited by Aβ 1-42 in PC12 cells. (rsc.org)
  • We report here that in an in vitro model for oxidative stress, H2O2 treatment, overexpression of TorsinA was protective against cell death. (fu-berlin.de)
  • nal cell damage in rat pheochromocytoma line 12 (PC-12) cells has been commonly used as an ideal model in vitro [5- (1library.net)
  • PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. (wikipedia.org)
  • One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is unclear. (nih.gov)
  • 04 +/- 0.5 ng of alpha-tubulin/mg of total protein) when compared with control cells. (biomedsearch.com)
  • SH2B adaptor protein family members (SH2B1-3) regulate various physiological responses through affecting signaling, gene expression, and cell adhesion. (sigmaaldrich.com)
  • Comparison of the effects of NGF, activators of protein kinase C and a calcium ionophore on the expression of Thy-1 and N-CAM in PC12 cell cultures. (springer.com)
  • We characterized a large protein complex containing a nerve-growth-factor-activated ERK (extracellular-signal-regulated kinase) and MEK (MAPK/ERK kinase) in rat pheochromocytoma (PC12) cells. (biochemj.org)
  • A role for zeta protein kinase C in nerve growth factor-induced differentiation of PC12 cells -- Wooten et al. (aacrjournals.org)
  • NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases. (rupress.org)
  • While the GRE-containing binding sites were more often located nearby genes involved in general cell functions and processes such as apoptosis, cell motion, protein dimerization activity and vasculature development, the binding sites without a GRE were located nearby genes with a clear role in neuronal processes such as neuron projection morphogenesis, neuron projection regeneration, synaptic transmission and catecholamine biosynthetic process. (biomedcentral.com)
  • The Schwann cell conditioned medium contains a protein factor (greater than 12 kDa and broken down by trypsin treatment) with remarkable thermal stability (unaffected at 95 degrees C for 15 min) and the ability to bind heparin. (ox.ac.uk)
  • Using western blot technique we observed that there is no change in NCS-1 and DARPP-32 protein levels in both PC12 WT and PC12 Clone cells after typical and atypical antipsychotic treatments. (biomedcentral.com)
  • Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. (royalholloway.ac.uk)
  • We examined NR1 splice variant and NR2 subunit expression in four PC12 cell-lines (ATCC, WEHI, Ordway and Flinders), correlated mRNA expression with protein expression, and used patch-clamp recordings to test functionality. (edu.au)
  • Synaptophysin, an integral membrane protein of small synaptic vesicles, was expressed by transfection in fibroblastic CHO-K1 cells. (elsevier.com)
  • The role of protein kinase A in hypoxia-induced PC-12 cell death. (louisville.edu)
  • Changes in the gene expression pattern of control versus optimedin in PC 12 cells were assessed using rat Affymetrix arrays. (arvojournals.org)
  • The insulinotropic hormone glucagon-like peptide-1 (7-36)-amide (GLP-1) has potent effects on glucose-dependent insulin secretion, insulin gene expression, and pancreatic islet cell formation and is presently in clinical trials as a therapy for type 2 diabetes mellitus. (aspetjournals.org)
  • In this study, Agilent rat oligo 22K microarrays were used to examine alterations in gene expression of PC12 cells after 500 μM MPP + treatment. (biomedcentral.com)
  • Relative gene expression of control and treated cells represented by spot intensities on the array chips was analyzed using bioinformatics tools. (biomedcentral.com)
  • The means of the averaged log ratio of the paired samples were used to identify the fold changes of gene expression in PC12 cells after MPP + treatment. (biomedcentral.com)
  • SIGNALS initiated by neurotransmitter release are transferred to the nuclei of the postsynaptic cells to activate specific programs of gene expression, thus influencing synaptic functions. (asahq.org)
  • Rikkunshito dose-dependently enhanced forskolin-induced elevations in cAMP in PC12 cells, and also increased the gene expression of TH and VIP. (biomedcentral.com)
  • We found that, when PC12 cells are treated with proNGF/NGF mixtures, a unique pattern of gene expression, which does not overlap with that deriving from treatment with either proNGF or NGF alone, is induced. (biomedcentral.com)
  • We have previously characterized the distinct properties of proNGF and NGF signalling by gene expression microarray in PC12 cells and identified two subsets of differentially expressed genes that could be ascribed to a "pure proNGF" and a "pure NGF" signalling, respectively [ 8 ]. (biomedcentral.com)
  • In conclusion, it was shown that PMMA has a minor impact and PDMS a major impact on gene expression in PC12 cells. (duhnnae.com)
  • These data suggest that GSH is involved in the granular storage of DA in PC12 cells and that, considering the molecular characteristics of the granular transport system, it is likely that GSH is used to protect susceptible parts of this system against (possibly DA-induced) oxidative damage. (jneurosci.org)