AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceNamed GroupsHealth Care
Leukocytes, MononuclearLymphocyte ActivationPhytohemagglutininsInterferon-gammaCells, CulturedT-LymphocytesMonocytesCytokinesInterleukin-2HIV-1Interleukin-10CD4-Positive T-LymphocytesFlow CytometryInterleukin-4Tumor Necrosis Factor-alphaHIV InfectionsCD8-Positive T-LymphocytesRNA, MessengerLymphocytesAntigens, CD3Cytotoxicity, ImmunologicInterleukin-12T-Lymphocyte SubsetsAntigens, CDEnzyme-Linked Immunosorbent AssayPolymerase Chain ReactionKiller Cells, NaturalVirus ReplicationCoculture TechniquesMolecular Sequence DataImmunophenotypingEpitopes, T-LymphocyteB-LymphocytesProvirusesImmunity, CellularDNA, ViralReceptors, Interleukin-2Interleukin-6Viral LoadReceptors, CCR5Interleukin-5Interleukin-1Concanavalin AT-Lymphocytes, CytotoxicCell DivisionPokeweed MitogensReverse Transcriptase Polymerase Chain ReactionMitogensTuberculinTh1 CellsImmunoglobulin GAntibodies, MonoclonalLymphocyte SubsetsImmunoglobulin ECell LineAntigens, Differentiation, T-LymphocyteImmunodeficiency Virus, FelineMembrane GlycoproteinsHLA-DR AntigensLipopolysaccharidesTh2 CellsBase SequenceRNA, ViralSimian immunodeficiency virusCell SeparationRecombinant ProteinsViremiaClone CellsDose-Response Relationship, ImmunologicInterleukinsAntigens, HelminthCytotoxicity Tests, ImmunologicGene ExpressionLymphocyte CountT-Lymphocytes, RegulatoryHIV Core Protein p24Antigens, BacterialBlood PreservationInterferon-alphaTetanus ToxoidTime FactorsEnzyme-Linked Immunospot AssayAmino Acid SequenceBlood CellsCell ProliferationLupus Erythematosus, SystemicAntigens, CD4Dendritic CellsSuperantigensAntigens, CD19Echinococcosis, PulmonaryGene Expression RegulationCase-Control StudiesCD4 Lymphocyte CountInterleukin-18Immune ToleranceAcquired Immunodeficiency SyndromeHIV SeropositivityEchinococcusReceptors, CXCR4
Mononuclear cellsPeripheralCellsWCGHDensity gradient centrifugationMixed immuneCellPatientsMarkersHumanFileDataChiefProductionAreasGroupsHealthyPeripheral Blood Mononuclear CellIsolation and CryopreservationViabilityPBMCsCerba ResearchDonorsVACCINEMethodsMonocytesClinical TrialsMiceAssaysConcentrationsProtocolBloodHumanImaging studiesSamplesResultsCrucialHigh-qualityCollectionMeetReadyMediumProjectStudyCostsModelsAddress
Mononuclear cells10
  • Human PBMC Genomic DNA was prepared from purified healthy human peripheral blood mononuclear cells (PBMCs). (3hbiomedical.com)
  • PBMC is an abbreviation for peripheral blood mononuclear cells. (hkadrfund.com)
  • In a previous posting , I discussed the use of T cell activation markers as a strategy for assessing the function of T cells from human peripheral blood mononuclear cells (PBMC ). (sanguinebio.com)
  • CTL's cryopreserved peripheral blood mononuclear cells (PBMC) are ideally suited for T cell monitoring in ELISPOT and ELISA assays, cytokine bead arrays, tetramer and pentamer staining, cytokine capture assays, and any assays that require live PBMC. (immunospot.eu)
  • Genes up-regulated in comparison of peripheral blood mononuclear cells (PBMC) from patients with acute influenza infection versus PBMC from patients with acute E. coli infection. (gsea-msigdb.org)
  • We defined 48 data sets from 13 different studies and developed single-cellderived-class' (SCDC) profiles representing multiple classes and subclasses of peripheral blood mononuclear cells (PBMC). (nottingham.edu.cn)
  • To determine the relatedness of the HIV strains from both persons, DNA was extracted from their peripheral blood mononuclear cells (PBMC). (cdc.gov)
  • Chemokine/cytokine responses in immune complex triggered IFN-α activity was studied in healthy donor peripheral blood mononuclear cells (PBMC). (lu.se)
  • The objective of the study was to assess the systemic effects of welding fume by examining molecular and cellular changes of isolated peripheral blood mononuclear cells (PBMC) in a rat model. (cdc.gov)
  • In one study, peripheral blood mononuclear cells (PBMC) from 6 of 7 patients with EMS and other functional somatic syndromes, when incubated with peak E, produced type II (profibrotic) cytokines (compared with 3 of 24 controls). (medscape.com)
Peripheral2
  • One milliliter of the human peripheral blood sample was prepared, and the PBMC sample was obtained by standard density gradient centrifugation technique using Histopaque-1083 (Sigma, #10831)1. (logosbio.com)
  • Here we illustrate the results of the cytokine release assays performed on the peripheral blood mononucleated cells (PBMC) isolated from the patients participating to the ZENITH trial. (unipi.it)
Cells8
  • Lymphocytes, monocytes, and Neutrophil Granulocytes in PBMC have very strong anti-tumor (cancer cells) and anti-viral effects! (hkadrfund.com)
  • However, T cells and NK (Natural Killer) cells are most important lymphoid in PBMC. (hkadrfund.com)
  • PBMC are component of white blood cells (WBC) consisting of select immune cells, including T cells, B cells, NK cells, and monocytes. (ignytebio.com)
  • In addition to the PBMC pools that are included in the CTL-MAT kits, we also offer EP 2.6.30 compliant cells, frozen according to our MAT optimized cryopreservation protocol. (ctlmat.com)
  • showed that PBMC T cells from metastatic melanoma patients had reduced CD71 upregulation compared with healthy controls, and this corresponded with multiple other functional defects in T cells from these patients. (sanguinebio.com)
  • has shown reduced upregulation of CD95 in PBMC T cells from breast cancer patients following T cell activation in the presence of interferons, indicating the lack of full T cell activation under these conditions. (sanguinebio.com)
  • Classification of cells by pattern recognition showed excellent performance for PBMC that were isolated, but not further processed by cell sorting. (nottingham.edu.cn)
  • Furthermore, PBMC derivatives such as T cells, B cells, NK cells, and stem cells are based on cell therapies, including CAR-T cell therapies and regenerative medicines. (logosbio.com)
WCGH1
  • Ellen Leone, RN, MSN, and chief nursing office for PBMC and WCGH, cited McDermott as an experienced leader who mentors many young leaders, and allows them to grow and develop in their own ways. (penbaypilot.com)
Density gradient centrifugation1
  • IGNYTE BIO PBMC are isolated from blood or apheresis material by density gradient centrifugation or negative selection. (ignytebio.com)
Mixed immune1
  • Therefore, PBMC is not a kind of cell, but a mixed immune cell. (hkadrfund.com)
Cell2
  • Defining Human PBMC T cell activation markers. (sanguinebio.com)
  • Understanding what these activation markers are, when they are expressed, and their role in T cell function during normal responses and disease states is important when selecting markers for assessing T cell biology for studies on human PBMC. (sanguinebio.com)
Patients1
  • After working with Northwell to expand its women's health program at South Shore University Hospital in Bay Shore, he was invited to do the same for the services Northwell and PBMC provide patients in eastern Suffolk County as managing director of women's health. (timesreview.com)
Markers1
  • Cr and Ni), that are absent in the GMA-MS fume, may enhance lung toxicity, as well as generation of oxidative- stress markers and DNA alterations in PBMC. (cdc.gov)
Human4
  • Product name: Human PBMC Genomic DNA. (3hbiomedical.com)
  • Here we provide the reference data files used to run the Azimuth Human PBMC reference web application. (zenodo.org)
  • These human PBMC samples are isolated from leukopacks and frozen in CTL-Cryo™ ABC serum-free freezing medium. (immunospot.eu)
  • The PBMC humanized mouse model for CRS assessment may be a valuable tool for preclinical assessment of CRS adverse events of experimental human therapeutic antibodies including TGN1412. (jax.org)
File1
  • We will get the 3k PBMC h5ad file from here and unzip. (lu.se)
Data2
  • To demonstrate this we will use the the Seurat pbmc_small example data. (lu.se)
  • If you point the OutPath to your CellexalVR Data path you will now have the Seurat pbmc_small data set in your VR environment. (lu.se)
Chief1
  • Shri P.B. Murugan, Chairperson PBMC, the chief guest on the occasion stressed on the importance of systematic solid waste management system with the support of public cooperation. (andamanchronicle.net)
Production1
  • Immune complex-stimulation of PBMC increased the production of CCL2, CXCL8 and TNF. (lu.se)
Areas1
  • The expansion will also include enhancing services at Northwell offices in areas surrounding PBMC, according to Dr. McKenna. (timesreview.com)
Groups1
  • ROS generation and P-HNE adduct formation increased at 14 h in the PBMC recovered from the MMA-SS group compared to other groups. (cdc.gov)
Healthy1
  • That is to say, PBMC is an immune lymphocyte derived from the blood of healthy animals. (hkadrfund.com)
Peripheral Blood Mononuclear Cell3
  • The Cross-Network Peripheral Blood Mononuclear Cell (PBMC) Processing Standard Operating Procedure (SOP) provides instructions for processing PBMC at network (ACTG, HPTN, HVTN, and IMPAACT) site-affiliated laboratories, and is intended to simplify and clarify PBMC processing, especially at shared sites. (hanc.info)
  • Objective: The objective of this study was to examine the dose-response relationship between As and global methylation of peripheral blood mononuclear cell (PBMC) DNA in apparently healthy Bangladeshi adults chronically exposed to a wide range of As concentrations in drinking water. (nih.gov)
  • The contract will establish a peripheral blood mononuclear cell (PBMC) bank and an EQA reagent repository of characterized material that supports EQAPOL proficiency testing programs and are made available to NIAID-supported investigators. (nih.gov)
Isolation and Cryopreservation1
  • In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation leading to reproducible, high-quality results. (genewiz.com)
Viability3
  • In addition, controlled-rate PBMC freezing can be performed in a low-endotoxin, serum-free and/or xeno-free cryopreservation medium to preserve their optimal viability and functionality upon thaw. (reprocell.com)
  • When blood was stored for 48 h before PBMC isolation, RBC contamination increased and post-thaw viability decreased in all three specimens. (nih.gov)
  • Post-thaw viability remained stable after an increased delay to PBMC isolation. (nih.gov)
PBMCs3
  • The ICCVAM BRD includes the ECVAM Standard Operating Procedures (SOPs) for the PBMC/IL-6 test (could be referred to as Monocyte Activation Test), which is based on various methods that use human PBMCs to detect cytokine production as a measure of pyrogen presence (Bleeker et al. (nih.gov)
  • For laboratories processing HVTN PBMCs, the laboratory must use the HANC PBMC SOP as written or the HVTN-specific version of the HANC SOP. (hanc.info)
  • The success of PBMC isolation was assessed macroscopically using PBMCs isolated from the blood of 3 donors that was stored for 2, 24, and 48 h before isolation using both a Ficoll gradient and a Leucosep tube. (nih.gov)
Cerba Research1
  • At Cerba Research we embrace the potential of PBMC activities to maximize impact in clinical research. (cerbaresearch.com)
Donors4
  • Further, leukocyte count declined and granulocyte contamination increased progressively with delay to PBMC isolation in specimens from all three donors. (nih.gov)
  • While the observed increase in granulocyte count with delayed PBMC isolation by Leucosep was confirmed in specimens from two donors using flow cytometry, the contamination was almost eliminated when RosetteSep treatment was performed. (nih.gov)
  • Results: Mice engrafted with PBMC derived from SSc patients developed autoantibodies such as antinuclear antibodies (ANA) mimicking the pattern of the respective donors. (uni-luebeck.de)
  • PBMC donors must meet the strict behavioral and medical history requirements as per applicable NCI-SB Apheresis Donor SOP(s). (nih.gov)
VACCINE1
  • PBMC samples were taken from 8 volunteers ages 20-49 enrolled in an HIV vaccine trial (NCT01578889). (ucsc.edu)
Methods2
  • Methods: PBMC derived from patients with SSc and GPA as well as healthy controls (HD) were isolated, characterized by flow cytometry, and infused into Rag2 -/- /IL2rg -/- mice. (uni-luebeck.de)
  • Methods: Global PBMC DNA methylation, plasma folate, blood S -adenosylmethionine (SAM), and concentrations of As in drinking water, blood, and urine were measured in 320 adults. (nih.gov)
Monocytes2
  • IGIV aggregates produced by mechanical stress induced higher cytokine release by PBMC and primary monocytes than aggregates formed by other stresses. (springer.com)
  • Results with the monocytic cell line THP-1 paralleled trends in PBMC and primary monocytes. (springer.com)
Clinical Trials1
  • PBMC isolation from adults is an important methodology to address complex immune responses and perform pharmacodynamic analyses in early phase clinical trials. (reprocell.com)
Mice4
  • Humanized PBMC mice are used as in vivo models to study and evaluate compounds for infectious diseases and graft rejection research. (jax.org)
  • In addition, PBMC from SSc patients treated with rituximab were transferred into mice. (uni-luebeck.de)
  • By contrast, PBMC derived from HD or GPA patients survived in recipient mice after transfer, but neither human autoantibodies nor inflammatory infiltrates in tissues were detected. (uni-luebeck.de)
  • Furthermore, these pathological changes were absent in mice transferred with PBMC from rituximab-treated SSc patients. (uni-luebeck.de)
Assays2
  • Immunofluorescence assays showed that TMEM147 was localized to the cell membrane and within the cell membrane in goat PBMC. (biomedcentral.com)
  • Impact of delayed PBMC processing on functional and genomic assays. (nih.gov)
Concentrations1
  • Conclusions: These results suggest that As is positively associated with global methylation of PBMC DNA over a wide range of drinking water As concentrations. (nih.gov)
Protocol1
  • If a protocol-specific PBMC worksheet is not required in the "HVTN Protocol Specific Processing Instructions," the generic worksheet below and in Appendix A of the SOP may be used. (hanc.info)
Blood1
  • To keep up with rising clinical research demand we perform various PBMC activities like isolation from whole blood samples and DNA extraction using advanced technologies and equipment. (cerbaresearch.com)
Human1
  • We observed that fluoride in small concentration may significantly decrease activity of 15LOX-1 and -2 in human PBMC macrophages and then concentration of its end products: 15-HETE, 12-HETE and 9+13-HODE, what may cause development of inflammation through the cholesterol arrest into the macrophages and its differentiation to foam cell. (fluoridealert.org)
Imaging studies1
  • The localization of TMEM147 in PBMC was explored by immunofluorescence in confocal imaging studies. (biomedcentral.com)
Samples1
  • Primate Biologicals is a leading supplier of Rhesus Monkey PBMC, and only provides samples originating from primates housed in the United States. (primatebiologicals.com)
Results3
  • Be sure to review section 5 Reporting Results for the new requirements for use of a PBMC Processing Worksheet and the LDMS. (hanc.info)
  • If the lab chooses to develop its own PBMC Processing Worksheet and supplementary tracking materials (such as the LDMS, or a separate worksheet or log) the laboratory will use the guidelines in section 5 Reporting Results of the SOP. (hanc.info)
  • Our results showed that TMEM147 was a binding partner of Hco-gal-m/f and mediated the immunological regulation of Hco-gal-m/f on goat PBMC in a manner different to that of TMEM63A. (biomedcentral.com)
Crucial1
  • PBMC-based analytical tests are crucial to accelerate the development of new vaccines, drug discovery, diagnostic development, Precision Medicine Research (eg. (pharmaceutical-technology.com)
High-quality2
  • Following PBMC and CBMC isolation, our experts are able to derive high quality low-passage progenitors such as EPCs in chemically defined cell culture system using xeno-free medium and perform subsequent mRNA reprogramming to iPSCs based on zero-footprint technology. (reprocell.com)
  • To this end, the supply of high-quality PBMC is an essential first step in the process. (pharmaceutical-technology.com)
Collection1
  • PBMC collection and resulting cell pellet. (anat.org.au)
Meet1
  • Several PBMC Processing Worksheet templates that meet the requirements are provided below. (hanc.info)
Ready1
Medium1
  • The PBMC layer is highlighted as a thin section between yellow plasma and clear separation medium. (anat.org.au)
Project1
  • PBMC, is a groundbreaking blockchain project focused on transforming the trade finance industry. (coinbackyard.com)
Study1
  • In this study, the functions of TMEM147 in the regulations of H. contortus galectin on the goat PBMC were investigated. (biomedcentral.com)
Costs1
  • PBMC processing costs and transport)? (nih.gov)
Models1
  • Categorical models indicated that estimated mean levels of PBMC DNA methylation were highest in participants with the highest As exposures. (nih.gov)
Address1
  • PBMC is a 2 days course that will help address the issue many people are facing after learning the art of property investment. (vickyhow.com)