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  • PAK1
  • Colocalization of activated Pak1 and paxillin at the leading edge of motile fibroblasts. (nih.gov)
  • full-length paxillin
  • Furthermore, replacement of full-length paxillin at adhesion sites by microinjected paxillin LIM2-LIM3 domains suppressed microtubule catastrophes exclusively at adhesions. (vanderbilt.edu)
  • By real-time PCR analysis we have established that overexpression of either full-length paxillin or a truncated nuclear form suppresses expression of the parental imprinted gene H19, and modulation of this locus probably affects the rate of NIH-3T3 cell proliferation. (biochemj.org)
  • binds paxillin
  • We have previously reported that the alpha(4) cytoplasmic domain directly and tightly binds paxillin, an intracellular signaling adaptor molecule, and this interaction accounts for some of the unusual functional responses to alpha(4) integrin-mediated cell adhesion. (nih.gov)
  • Here, we report that kindlin-2 has a dual role during initial cell spreading: it binds paxillin via the pleckstrin homology and F0 domains to activate Rac1, and it directly associates with the Arp2/3 complex to induce Rac1-mediated membrane protrusions. (rupress.org)
  • Furthermore
  • Furthermore, when expressed in cells, this alpha(4)-binding paxillin fragment specifically inhibited the alpha(4) tail-stimulated cell migration. (nih.gov)
  • Furthermore, NMR studies using LD-containing peptides showed predominant interactions between LD3 and LD4 of paxillin and α4 CT. (ntu.edu.sg)
  • Furthermore, there was a significant correlation between fascin-1 expression with paxillin immunostaining but no such association with PR or ER status. (begellhouse.com)
  • adaptor
  • Principal Findings: Using NMR spectroscopy, we determined the 3-D structure of the full-length α4 CT (Lys968-Asp999) and characterize its interactions with the adaptor protein paxillin. (ntu.edu.sg)
  • Significance: The current study provides molecular insights into the structural diversity of α CTs of integrins and interactions of integrin α4 CT with the adaptor protein paxillin. (ntu.edu.sg)
  • interaction
  • We also have identified a conserved 9-amino acid region (Glu(983)-Tyr(991)) in the alpha(4) cytoplasmic domain that is sufficient for paxillin binding, and an alanine substitution at either Glu(983) or Tyr(991) within this region disrupted the alpha(4)-paxillin interaction and reversed the effects of the alpha(4) cytoplasmic domain on cell spreading and migration. (nih.gov)
  • Thus, paxillin binding to the alpha(4) tail leads to enhanced cell migration and inhibition of the alpha(4)-paxillin interaction selectively blocks the alpha4-dependent cellular responses. (nih.gov)
  • Paxillin contains several important interaction domains. (biologists.org)
  • NMR titration data, using a synthetic paxillin LD1 peptide, revealed a binding interaction site within PABP-1 RRM 2 and not within the PABP-1 RRM 1 domain. (le.ac.uk)
  • Gene
  • In lung cancer tissues, we have found that paxillin was highly expressed (compared with normal lung), amplified (12.1%, 8 of 66) and correlated with increased MET and epidermal growth factor receptor ( EGFR ) gene copy numbers, or mutated (somatic mutation rate of 9.4%, 18 of 191). (aacrjournals.org)
  • Gene silencing from RNA interference of mutant paxillin led to reduction of cell viability. (aacrjournals.org)
  • Gene disruption of paxillin in mice affects the development of both extraembryonic and embryonic structures in mice. (aacrjournals.org)
  • Therefore, the aim of this study was to study the effects of in vivo endothelial NO synthase (eNOS) gene transfection on paxillin expression and intimal hyperplasia. (ahajournals.org)
  • eNOS gene transfer showed no effect on paxillin downregulation 2 days after injury but significantly enhanced the recovery of paxillin protein 5 days and 2 weeks after injury. (ahajournals.org)
  • constructs
  • Using overlapping PABP-1 and paxillin constructs it was found that one of paxillins N-terminal LD domains (LD1) interacted with PABP-1 RRM (RNA recognition motif) 1 and 2. (le.ac.uk)
  • Methods
  • Methods A total of 386 tissue specimens from PCa patients who received radical prostatectomy and 60 tissue specimens from benign prostatic hyperplasia (BPH) cases were collected to construct tissue microarrays, which were subsequently immunostained for Paxillin expression. (jcancer.org)
  • cells
  • 14 Rhombotin-2 is required for erythroid differentiation during mouse development, 15 and paxillin has also been found to be associated with the differentiation of HL-60 and neuronal cells. (ahajournals.org)
  • metastasis
  • This study analyzed the association between Paxillin expression in prostate cancer (PCa) tissues with clinicopathological features, lymph node metastasis and biochemical PCa recurrence. (jcancer.org)
  • Results The association between Paxillin expression, lymph node metastasis and biochemical PCa recurrence was analyzed. (jcancer.org)
  • The receiver operating characteristic (ROC) curve indicated that Paxillin expression (AUC=0.723) more accurately predicted PCa lymph node metastasis than Gleason score (AUC=0.692). (jcancer.org)