Patient tissue array analysis. Medical search. Frequent questions

In the tissue microarray technique, a hollow needle is used to remove tissue cores as small as 0.6 mm in diameter from regions of interest in paraffin-embedded tissues such as clinical biopsies or tumor samples. (wikipedia.org)
The overall goals of this core is to provide a centralized resource for acquisition, logging, processing, storage and analysis of plasma samples and tissues biopsies. (grantome.com)
Tissue microarray slides (TMAs) are often used in spatial analysis as a surrogate for whole-slide patient biopsies. (selectscience.net)
Two aldehyde groups are significantly enriched in EAC biopsies and adjacent tissue: (i) short-chain alkanals, and (ii) medium-chain alkanals, including decanal. (nature.com)
Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
We successfully detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) cases of WD. (frontiersin.org)
Anatomical pathology equipment and consumables help in analysing blood or other fluids from patients, and evaluate tissue specimens that include biopsies, surgical resections, cervical scrapings, and whole-body evaluations at autopsy. (thebusinessresearchcompany.com)

Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis. (muni.cz)
Prognostic models based on immune-related genes help to improve the prognosis prediction and clinical treatment of breast cancer patients. (techscience.com)
The immune-related gene signature effectively predicts the survival and immune infiltration of breast cancer patients and is expected to provide more effective immunotherapy targets for the prognosis prediction of breast cancer. (techscience.com)
Background: Vestigial like family member 3 (VGLL3) is associated with the prognosis of epithelial ovarian cancer and soft tissue sarcoma, but its role in gastric cancer (GC) is unclear. (researchgate.net)
The correlation of VGLL3 expression status with patient prognosis was then determined by univariate and multivariate Cox regression analyses. (researchgate.net)
Kaplan-Meier analysis showed that the high VGLL3 expression group had a worse prognosis compared to the low expression group (P = 0.019). (researchgate.net)
Multivariate analysis showed that VGLL3 expression status was an independent risk factor for prognosis. (researchgate.net)
Conclusion: VGLL3 is overexpressed in GC tissues and associated with a poor prognosis, indicating its potential as a novel prognosis biomarker and therapeutic target for GC. (researchgate.net)
The mRNA levels of Gli2 and MMP-7 in normal ovarian tissues and cancerous tissues in various stages together with the corresponding clinical information were acquired from the indicated GEO datasets to elucidate associations between MMP-7 expression and cancer progression and prognosis. (jcancer.org)
Disease-free survival (DFS) was significantly shorter in patients with high expression of CRABP1, and multivariate analysis of DFS revealed that high expression of CRABP1 in the tumor area and lymph node metastasis served as an independent factor associated with poor prognosis. (nagoya-u.ac.jp)
As SCC is often curable if detected and treated early, a better understanding of the origin of SCC within the epithelium is also likely to lead to improvements in early detection of the disease and, ultimately, improved patient prognosis and quality of life. (ukri.org)
Patients with Wilms tumor (WT) in general have excellent survival, but the prognosis of patients belonging to the subgroup of WT with diffuse anaplasia (DA) is poor due to frequent resistance to chemotherapy. (lu.se)
The risk of distant recurrence in breast cancer patients is difficult to prognostic indices, such as the Nottingham Prognostic Index (6, 7), assess with current clinical and histopathological parameters, and have proven valuable in identifying patients with poor prognosis. (lu.se)

For each patient, the team performed exome sequencing of the tumors and peripheral blood mononuclear cells, as well as mRNA sequencing of the paired tumor and adjacent normal tissues. (genomeweb.com)
Current projects involve the construction of tissue arrays of plasma cell tumors for immunohistochemical and in-situ hybridization assays. (ucsf.edu)
Tissues from primary tumors and metastases as well as blood samples were collected. (uib.no)
In November 2020, Roche launched Blood-based biomarker FoundationOneLiquid CDx for comprehensive pan-tumor liquid biopsy test for patients with solid tumors in India. (thebusinessresearchcompany.com)
Our findings show that the application of array-CGH allows to delineate clearly the genomic profiles of WDLPS, DDLPS and MLPS that reflect biological differences between these tumors. (biomedcentral.com)
We also attempt to investigate EGFL6 gene expression in brain arachnoidal tissues and various tumors and to measure EGFL6 levels in serum samples from healthy people and patients with various tumors by using ELISA. (omicsdi.org)
EGFL6 mRNA levels were also determined in brain arachnoidal tissues, meningiomas, and other tumors by qRT-PCR. (omicsdi.org)
EGFL6 levels were measured in serum samples from healthy people and patients with various tumors by using ELISA. (omicsdi.org)
Patients with all other investigated tumors, however, had low levels of serum EGFL6 with mean concentration less than 240 pg/ml. (omicsdi.org)

Additionally, immunohistochemistry (IHC) was performed in multiple ovarian cancers, benign tumours and normal tissues to evaluate Gli2 and MMP-7 protein expression. (jcancer.org)
The researchers collected paired tumor and adjacent normal tissues, as well as blood samples, from 80 patients with EOGCs under 45 years of age. (genomeweb.com)
The researchers also performed global proteome, phosphoproteome, and N-glycoproteome profiling of paired tumor and adjacent normal tissues using liquid chromatography-tandem MS analyses. (genomeweb.com)

Until recently, genomic technologies were not available for testing of preserved POC tissue samples, but that has changed, opening up the possibility of adding chromosomal array or other genomic analyses to the current cadre of recommended tests, Maslow said. (genomeweb.com)
The researchers also compared previous genomic analyses of TCGA and Hong Kong (HK) GC cohorts - which mostly included late-onset GCs - to their own data and found that the EOGCs showed a different mutation landscape than the late-onset cancers (LOGCs). (genomeweb.com)

In addition, an independent cohort of 55 clinical samples was analyzed by quantitative real-time PCR analysis. (cancerindex.org)
The results were validated both by gene expression analysis and immunohistochemistry study in an independent 55 clinical samples from Fudan University Shanghai Cancer Center. (cancerindex.org)
The major limitations in molecular clinical analysis of tissues include the cumbersome nature of procedures, limited availability of diagnostic reagents and limited patient sample size. (wikipedia.org)
The possibility to assemble a large number of representative cancer samples from a defined patient cohort that also has a corresponding clinical database, provides a powerful resource to study how different protein expression patterns correlate with different clinical parameters. (wikipedia.org)
Clinical cancer patient cohorts and corresponding tissue microarray sets have been used to study diagnostic, prognostic and treatment predictive cancer biomarkers in most forms of cancer, including lung, breast, colorectal and renal cell cancer. (wikipedia.org)
Specific aims of this core are: 1) To provide a centralized facility to support standardized ancillary studies for several human clinical trials, canine trial, and rodent studies and examine tissue and plasma-based parameters related to tumor hypoxia, angiogenesis, and acidosis. (grantome.com)
Gene array analysis will be performed on tissues obtained from human and canine patients enrolled in clinical trials. (grantome.com)
Dr. Zbigniew Mikulski outlines the dynamic evolution of microscopy, highlighting its capacity to provide high-resolution quantitative data tailored for translational and clinical spatial analysis. (selectscience.net)
Baseline characteristics, clinical efficacy, complications, peritoneal recurrence and metastasis, disease-free survival (DFS), and overall survival (OS) of the patients, as well as their quality of life (QoL) after chemotherapy, were compared between groups. (bvsalud.org)
INTRODUCTION: Organ preservation is now considered an acceptable alternative option in distal rectal cancer patients with clinical complete response (cCR) after neoadjuvant chemoradiation (CRT). (bvsalud.org)
Tissue and breath concentrations of the medium-chain alkanal decanal are correlated, and increased decanal is linked to reduced ALDH3A2 expression, TP53 deletion, and adverse clinical features. (nature.com)
We show that FISH not only detect active WD but also be helpful as an indicator for the efficiency of antibiotic treatment and for detection of recurrence of disease when the signal of PAS diastase and immunohistochemistry lags behind the recurrence of disease, especially if the clinical course of the patient and antimicrobial treatment is considered. (frontiersin.org)
For a more comprehensive and unbiased proteomic characterization, the Clinical Proteomic Tumor Analysis Consortium performed mass spectrometry-based proteomic and/or phosphoproteomic analyses of tumor tissues from patients with colon, breast, and ovarian cancers, which demonstrated that proteomic signatures can provide information that can further stratify patients. (genomeweb.com)
Purpose: This PhD project is based on a clinical Phase II trial that investigated efficacy and safety of bevacizumab monotherapy in patients with metastatic melanoma. (uib.no)
Material and Methods: 35 patients with metastatic melanoma in progression were included in this clinical Phase II trial conducted at Haukeland University Hospital. (uib.no)
RESULTS: Pre-existing protocols for fresh tissue, plasma and urine collection have been incorporated within CRUD via a tissue-tracking system, to comply with the Human Tissue Act 2004, and link these samples to accurate clinical, pathological and survival data. (ox.ac.uk)
TPC team members work with USC Norris investigators on the planning, design, and feasibility of cancer research studies requiring human biospecimens and play a key role in procuring tissue for USC Norris clinical trials. (usc.edu)
Liposarcomas (LPS), the most common soft tissue sarcomas accounting for less than 1% of all human cancer cases, display remarkable clinical and pathological heterogeneity. (biomedcentral.com)
Reporting by clinical laboratories is upon completion of an analysis of a blood cholinesterase test that is below the normal range (an effect of the so-called cholinesterase inhibiting pesticides in the organophosphate and carbamate chemical classes), or tissue analysis for pesticide residues. (cdc.gov)
14: Laurell H, Hansson LE, Gunnarsson U. Impact of clinical experience and diagnostic performance in patients with acute abdominal pain. (cancercentrum.se)
Several single gene disorders share clinical and radiologic characteristics with multiple sclerosis and have the potential to be overlooked in the differential diagnostic evaluation of both adult and paediatric patients with multiple sclerosis. (medscape.com)
Here we review single gene disorders that have the potential to mimic multiple sclerosis, provide an overview of clinical and investigational characteristics of each disorder, and present guidelines for when clinicians should suspect an underlying heritable disorder that requires diagnostic confirmation in a patient with a definite or probable diagnosis of multiple sclerosis. (medscape.com)
Patients with multiple sclerosis are classified according to their clinical phenotype, with ~85% following a relapsing-remitting course (relapsing-remitting multiple sclerosis) characterized by recurrent, acute neurological deficits punctuating periods of latency or remission (Lublin and Reingold, 1996). (medscape.com)
In the absence of pathognomonic clinical findings or a definitive laboratory test, the diagnosis of multiple sclerosis remains challenging in many patients, and diagnostic criteria emphasize the caveat of 'no better explanation' for a patient's clinical presentation and MRI findings. (medscape.com)

The intense, focused light of lasers with these colors can be used for a wide variety of unique therapeutic interventions: making incisions, cauterizing and sealing wounds, and selectively heating or even vaporizing specific regions of organs and tissues. (nationalacademies.org)
WD is a multi-systemic disease with manifestation in various extra-intestinal organs including the central nervous system (CNS) (10-50% of WD patients), the heart (endocarditis), joints (isolated arthritis, spondylodiscitis), the eye (uveitis), and lung (pneumonia) ( 7 ). (frontiersin.org)
Anatomic pathology is a branch of science in which the diagnosis of the disease is carried out by examining tissues and organs at a molecular, biochemical, and microscopic level. (thebusinessresearchcompany.com)
Epithelial tissue lines the surface of the body (i.e. the skin), along organs and glands inside it. (ukri.org)
Even if hair analysis was a highly reproducible and valid test, done properly, there are virtually no data for correlation of findings with levels of elemental minerals found in other tissues or organs. (cdc.gov)
Cells Tissues Organs. (cancercentrum.se)

19 formalin-fixed paraffin-embedded (FFPE) duodenal biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD were retrospectively examined using PAS diastase staining, immunohistochemistry, and FISH. (frontiersin.org)
Procurement, processing, tracking, and distribution of formalin-fixed, paraffin embedded (FFPE) tumor tissue from USC Norris hospitals including Los Angeles General Medical Center, Keck Hospital, Norris Cancer Hospital, and CHLA, as well as other catchment area hospitals through the RTR. (usc.edu)

Year-1: Validation of HNMT as a predictive marker for Herceptin-Resistance (HR) in breast cancer patients Aim 1: Determination of the HNMT and HER2 mRNA expression level in BC cells 1. (tmu.edu.tw)
The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
The correlations of CRABP1 mRNA expression in tissues with clinicopathological factors of 230 patients who underwent radical gastrectomy were further evaluated. (nagoya-u.ac.jp)

Tissue microarrays (also TMAs) consist of paraffin blocks in which up to 1000 separate tissue cores are assembled in array fashion to allow multiplex histological analysis. (wikipedia.org)
Tissue microarrays are particularly useful in analysis of cancer samples. (wikipedia.org)
The use of tissue microarrays in combination with immunohistochemistry has been a preferred method to study and validate cancer biomarkers in various defined cancer patient cohorts. (wikipedia.org)
Immunohistochemistry combined with tissue microarrays has also been used with success in large scale efforts to create a map of protein expression on a more global scale. (wikipedia.org)
PHH3, MCM4 and mitosin were examined by staining of tissue microarrays (TMA) sections. (biomedcentral.com)
The five selected proteins (MKI67, EZH2, SLC2A1, CADM1, and NKX2-1 alias TTF1) were analyzed by immunohistochemistry on tissue microarrays including tissue from 326 non-small cell lung cancer patients. (nih.gov)
The prognostic impact of one of these genes, the cell adhesion molecule 1 (CADM1), was confirmed by use of immunohistochemistry on tissue microarrays from 2 independent NSCLC cohorts, altogether including 617 NSCLC samples. (scilifelab.se)
Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)

These dyes or biomarkers can be used in conjunction with visible and near-infrared light to highlight specific cell and tissue types, such as metastatic cancer cells circulating in the bloodstream. (nationalacademies.org)
Due to the lack of clear symptoms and specific biomarkers, most HCC patients are diagnosed in the late stage of disease, and patients with advanced HCC are generally not eligible for surgical treatment and have short survival periods, usually only 6 months [ 2 ]. (jcancer.org)
We first asked whether a cell-autonomous mechanism could contribute to exhaled biomarkers, so we began by searching for thematic differences in metabolic gene expression between EAC and relevant control tissue in archived transcriptomic datasets. (nature.com)
The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)

In addition, the in situ expression of VGLL3 in the GC tissues was determined by immunohistochemistry (IHC), and the patients were accordingly classified into the high and low expression groups. (researchgate.net)

College of Wisconsin, current era from 28 patients with septal defects who factor HEY2 in formalin fixed tissue taken from a Milwaukee, Wisconsin, USA underwent cardiac surgery and who were enrolled in our collection of hearts with atrial septal defects congenital heart disease tissue bank. (cdc.gov)

Recent research has classified lung adenocarcinoma patients with KRAS mutation into three subtypes by co-occurring genetic events in TP53 (KP subgroup), STK11/LKB1 (KL subgroup) and CDKN2A/B inactivation plus TTF-1 low expression (KC subgroup). (cancerindex.org)
We will combine our mRNAs expression profile (6 TAD tissues versus 6 non-TAD tissues) and GSE52093 downloaded from the Gene Expression Omnibus (GEO) database. (hindawi.com)
The two mRNAs expression profiles contained 13 TAD aortic tissues and 11 non-TAD tissues. (hindawi.com)
With the development of the gene expression profile, comparisons of differentially expressed genes that participate in the regulation of pathophysiological conditions in pathological tissue and normal tissue were available and convenient, and mRNAs gene expression profiles have been carried out in many diseases including TAD [ 6 ]. (hindawi.com)
In the present study, we use mRNA microarray to acquire differential expression profiles in human TAD tissues and non-TAD tissues. (hindawi.com)
3) Gene expression analysis will also be used to assess changes in gene expression early in the course of therapy to ascertain which types of genes are altered in response to therapies involving combinations of hyperthermia with radiotherapy and/or chemotherapy. (grantome.com)
Robust measurements can provide definitive evidence for the role of molecules in pathogenesis, such as the degree of expression of antigen-presenting proteins across pancreatic islets of patients with Type 1 Diabetes (T1D) 2 . (selectscience.net)
Methylation, expression, microsatellite marker, single nucleotide polymorphism array and trio whole-exome sequencing analyses were conducted. (bmj.com)
Tissue-array analysis in HR and HS tumor tissues to prove the correlation between HR and the p-STAT3/p-HNMT expression level. (tmu.edu.tw)
3. Tissue microarray analysis for the HNMT/STAT3 expression in the animal samples. (tmu.edu.tw)
The expression of MMR genes (MSH2, MLH1, MSH6 and PSM2) and co-stimulatory molecule CD80 was assessed by tissue microarray immunohistochemistry. (oncotarget.com)
Results: Patients with MMR-D showed a higher T-bet/CD4 ratio ( p = 0.02), a higher rate of CD80 expression and CD8 lymphocyte infiltration compared to those with no MMR-D. Moreover, in the MMR-D group, the Treg marker FoxP-3 was not expressed ( p = 0.05). (oncotarget.com)
MMR-D patients with stage I or II and T-bet expression had a significant better survival ( p = 0.009). (oncotarget.com)
In the study, we used weighted gene co-expression network analysis to construct a co-expression network to screen out highly prognostic immune-related genes. (techscience.com)
Univariate analyses showed a worse outcome in cases with elevated Ki-67 expression and high mitotic count, whereas PHH3, MCM4 and mitosin were not significant. (biomedcentral.com)
S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors (TFs) are the primary elements. (biomedcentral.com)
The expression levels of VGLL3 mRNA and protein were analyzed in the freshly resected tumor and normal gastric tissues from GC patients by quantitative RT-PCR and Western blot, respectively. (researchgate.net)
Through database analysis, we found that PAK3 was highly expressed in HCC patients and was positively correlated with tumor stage and grade, suggesting that PAK3 expression was closely related to HCC occurrence and development. (jcancer.org)
Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
IHC analysis demonstrated abnormally increased Gli2 and MMP-7 expression levels in benign tumours and ovarian cancer tissues. (jcancer.org)
The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
Reverse transcription-quantitative (RT-q) PCR and a PCR-array method were used to determine whether the expression of CRABP1 mRNA in gastric cancer cell lines correlated with the expression of cancer-related genes. (nagoya-u.ac.jp)
High expression of CRABP1 in cancer tissues was associated with a greater incidence of peritoneal recurrences after curative gastrectomy. (nagoya-u.ac.jp)
Biomarker discovery in non-small cell lung cancer: integrating gene expression profiling, meta-analysis, and tissue microarray validation. (scilifelab.se)
Gene expression array data were used as a training set to screen for single genes with prognostic impact. (scilifelab.se)
Low CADM1 protein expression was significantly associated with shorter survival, with particular influence in the adenocarcinoma patient subgroup. (scilifelab.se)
In tumor samples from patients with ICC, increased TAN and TAM levels were correlated with elevated p-STAT3 expression. (bmj.com)
Public available gene expression datasets were used for therapeutic response rate analysis. (biomedcentral.com)
Experimental Design: Differential gene expression profiles between meningiomas and brain arachnoidal tissues were established by using Affymetrix GeneChip Human U133 Plus 2.0 Array. (omicsdi.org)
Differential gene expression profiles between meningiomas and brain arachnoidal tissues were established by using Affymetrix GeneChip Human U133 Plus 2.0 Array. (omicsdi.org)
Genome wide DNA alterations were evaluated by array CGH in addition to RNA expression profiling in colorectal cancer from patients with excellent and poor survival following primary operations. (lu.se)
Global RNA expression was determined by 44K arrays. (lu.se)

Design and construction of tissue micro-arrays (TMAs) including a new automated TMA instrument. (usc.edu)

In their study, Maslow and her co-investigators took the results of Natera's SNP-based chromosomal array analysis, and results of the standard screening methods for their cohort of 42 couples, defined a detection rate for each, and then compared them. (genomeweb.com)
0.001) in a cohort of 30 GC patients. (researchgate.net)
0.05) in a cohort of 172 patients. (researchgate.net)
The best-performing model was subsequently validated in an independent cohort consisting of tissue from 345 non-small cell lung cancer patients. (nih.gov)
Using a novel NSCLC cohort together with a meta-analysis validation approach, we have identified a set of single genes with independent prognostic impact. (scilifelab.se)

Then we downloaded another microarray dataset GSE52093 [ 9 ] and screened out the coexpression of differentially expressed genes (DEGs) between TAD patients and non-TAD patients. (hindawi.com)

3. Development of the Chip-array diagnosis kit to help clinicians to select responders for Herceptin therapy. (tmu.edu.tw)
Volatile aldehydes are enriched in esophageal adenocarcinoma (EAC) patients' breath and could improve early diagnosis, however the mechanisms of their production are unknown. (nature.com)
These results support EAC early diagnosis trials using exhaled aldehyde analysis. (nature.com)
With not only insidious early symptoms but also limited treatment strategies, approximately 60% of ovarian cancer patients present with distant metastasis upon initial diagnosis, and their overall 5-year relative survival rate is a miserable 46% due to clinically occult dissemination and metastasis [ 5 ]. (jcancer.org)
With this in mind, the development of the brush biopsy (Oral CDx R) coupled with a computer-assisted method of analysis has brought accurate diagnosis, ease of performance, and patient acceptance into daily practice. (medscape.com)
Neither CT nor MRI is tissue specific, and tissue diagnosis may be required. (medscape.com)
Diagnosis is by cerebrospinal fluid (CSF) analysis. (msdmanuals.com)
Patients with confirmed idiopathic PD diagnosis living in the region of Skåne are invited to participate. (lu.se)
Early, accurate diagnosis is critical to effective patient management and counselling, but assignment of an incorrect diagnosis of multiple sclerosis remains a frequent concern. (medscape.com)

Traditional bioinformatics methods including gene ontology (GO) and pathways enrichment analyses of DEGs combined protein-protein interaction (PPI) network and cytohubber calculating software as well as Molecular Complex Detection (MCODE) analysis were used to analyze the key gene and pathway. (hindawi.com)
Patients and Methods: A group of 113 consecutive patients operated for CRC (42 stage I or II and 71 with stage III or IV) was retrospectively analyzed. (oncotarget.com)
PATIENTS AND METHODS: A total of 286 patients with primary GC admitted to the hospital from March 2017 to December 2020 were recruited in the study. (bvsalud.org)
METHODS AND ANALYSIS: This prospective, monocentric, open-label, single-arm phase II study will include 32 patients with cT3-4NanyM0 distal rectal adenocarcinoma. (bvsalud.org)
Methods: Integrative analysis was performed on the GC transcriptome profiles and survival information deposited in the ONCOMINE, GEPIA, and ONCOLNC databases. (researchgate.net)
PATIENTS AND METHODS: Immunohistochemistry for dMMR and pyrosequencing for KRAS/BRAF were performed for 1,913 patients randomly assigned between fluorouracil and folinic acid chemotherapy and no chemotherapy in the Quick and Simple and Reliable (QUASAR) trial. (ox.ac.uk)
Methods: Transcriptome analysis of surgically resected metastatic tissue from GC patients and noncancerous tissue was performed to identify novel metastasis-related genes. (nagoya-u.ac.jp)
METHODS: Links have been established between pathological databases, multidisciplinary team data-collection systems and patient-survival monitoring facilities, providing accurate pathology, treatment and survival data on all of uro-oncology patients. (ox.ac.uk)
Methods We explored the distributions of TANs and TAMs in patient-derived ICC samples by multiplex immunofluorescent staining and tested their separate and combined effects on ICC in vitro and in vivo. (bmj.com)
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient. (lookformedical.com)
In recent years, the application of high-resolution methods, such as array-CGH, results in a significant progress in the whole-genome analysis. (biomedcentral.com)

Finally, we validated our results in a tissue microarray composed of primary tumor tissues from 359 patients with ICC. (bmj.com)
before resection of the primary tumor and then postoperatively every 6-12 mo, resulting in three to five samples per patient. (lu.se)

Module analysis revealed that TAD was associated with significant pathways including cell cycle, vascular smooth muscle contraction, and adrenergic signaling in cardiomyocytes. (hindawi.com)
3. Study the action of Herceptin-induced HNMT overexpression and the mechanisms that cause HR. Aim 3: Establish HNMT inhibition (siRNA) and overexpression cell lines to clarify the HR-related signaling pathways Year-2 proposal: Study the mechanisms of HNMT-mediated gene regulation in the HR cells Aim 1: Establish the HR cells for transcriptome sequencing analysis 1. (tmu.edu.tw)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In addition, gene set enrichment analysis shows 6 pathways that differ between high- and low-risk group. (techscience.com)
KEGG pathway analysis was performed to identify potential gene pathways that may be involved in the pathogenesis of meningiomas. (omicsdi.org)
KEGG analysis also demonstrated that focal adhesion and ECM-receptor interaction pathways were activated in anaplastic meningioma. (omicsdi.org)

The introduction of a national program in Israel for exome sequencing for global developmental delay has transformed patient care, leading to a significant effect on medical management and treatment. (cdc.gov)

HNMT and HER2 were detected simultaneously (*p=0.001) in the same patients. (tmu.edu.tw)

Non parametric statistics and survival analysis were used. (oncotarget.com)
Survival analysis and time receiver operating characteristic curves indicate that the prognostic signature has strong predictive performance. (techscience.com)
PHH3, MCM4 and mitosin did not predict patient survival. (biomedcentral.com)
Moreover, high MMP-7 levels were significantly associated with poor overall survival (OS) and poor progression-free survival (PFS) in ovarian cancer patients. (jcancer.org)
In previous studies of GCs, genomic and transcriptomic analyses have identified molecular signatures associated with phenotypes of the disease, such as patient subtypes and survival. (genomeweb.com)
DNA and RNA from tumor and normal colon were used from cancer patients grouped according to death, survival or Dukes A, B, C and D tumor stage. (lu.se)
Mathematical Vector analysis implied a number of BAC-clones in tumor DNA with genes of potential importance for death or survival. (lu.se)

In contrast to the sampling of cells of the uterine cervix, analysis of surface epithelial cells of the oral cavity and oropharynx by standard exfoliative cytology has proven to be unreliable in identifying as many as 31% of dysplastic lesions as demonstrated in one study. (medscape.com)
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. (lookformedical.com)

The gene ontology and pathway enrichment of DEGs were performed by DAVID and Kyoto Encyclopedia of Genes and Genomes online analyses, respectively. (hindawi.com)
Enrichment analysis was performed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. (nature.com)

Lectin arrays are widely used tools for analyzing glycosylation in proteins and cells. (muni.cz)
Furthermore, we investigated 60 angiogenesis-related proteins in serum samples by a multiplex array. (uib.no)
Unsupervised hierarchical clustering as well as supervised analysis for particular proteins were performed. (uib.no)
Proteins with a defined fold change difference between responders and non-responders were further investigated by specific analysis. (uib.no)
Single protein ELISA was performed for these candidate proteins but could not confirm the results from the multiplex array. (uib.no)
Two high density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA, and 25 healthy controls. (ox.ac.uk)
This risk assessment was not dependent on the type of adju- decoding patterns of immunoregulatory serum proteins could re- vant therapy received by the patients. (lu.se)

Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. (wikipedia.org)
Tissues will be used for for immunohistochemical evaluation of the hypoxia-regulated protein, CAIX (carbonic anhydrase, isoform IX), microvessel density, and in situ measurement of tissue lactate concentrations using bioluminescence imaging. (grantome.com)
Finally, I am finishing testicular germ cell tumor tissue array for immunohistochemical and in-situ hybridization assays to help classify and identify cytogenetic abnormalities that are diagnostically and prognostically significant. (ucsf.edu)
We performed immunohistochemical staining of whole tissue sections from primary melanomas and metastases. (uib.no)

Baehner F, Balassanian R, MacGrane G and Waldman F. Donor origin of neuroendocrine carcinoma in two transplant patients determined by molecular cytogenetics, Hum Pathol. (ucsf.edu)
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. (lookformedical.com)

The scanner that records the intensity value is linked to digital image analysis software, which produces a color-coded image of the array, and a quantitative value is recorded for each target gene. (medscape.com)
When hair analyses have been done rigorously in quantitative laboratory settings, it has been pointed out that great care must be taken to avoid possible sources of contamination. (cdc.gov)

There are 12 samples (6 TAD and 6 NT) that were used for microarray analysis by Arraystar Human mRNA microarray which contained 14596 mRNAs probes. (hindawi.com)
Aim-3: HNMT as a predictive biomarker to accurately select responders for Herceptin® Tissue microarray analysis will be performed to determine the following: 1. (tmu.edu.tw)

Results: Analysis of the ONCOMINE and GEPIA databases showed that VGLL3 was significantly up-regulated in GC tissues (P = 0.003), and associated with the tumor TNM stage (P = 0.0163). (researchgate.net)
The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)

Based on the encouraging results of a disease control rate of 31%, we wanted to investigate predictive markers in tissues and blood samples. (uib.no)

Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. (muni.cz)

0.01) were tested in a meta-analysis including five publicly available independent lung cancer cohorts (n = 860). (scilifelab.se)

Since patient samples are assembled into the same block, sections can be stained with the same protocol to avoid experimental variability and technical artefacts. (wikipedia.org)
Rather than investigating an entire tissue section, small regions are selected, punched out, and assembled into an array, allowing multiple samples to be analyzed at once. (selectscience.net)
Results of the study, published last month in the Journal Obstetrics and Gynecology , also suggested that the routine use of SNP arrays to test preserved products of conception (POC) samples could help rule out cases of pregnancy loss due to fetal aneuploidy, and thus increase the diagnostic yield of follow-on screening tests used to provide answers to couples with unexplained multiple lost pregnancies. (genomeweb.com)
Retrospectively, the investigators compared results of the standard recommended screening process and the results of SNP array testing of FFPE POC samples for a group of 42 women in order to determine their relative detection rates. (genomeweb.com)
During the study's recruitment, physicians at the UConn Center for Advanced Reproductive Services began routinely requesting FFPE POC samples from patients, and when available, sending these for SNP array analysis by Natera, which offers a POC testing service it calls Anora. (genomeweb.com)
While UConn has adopted Natera's test, Maslow stressed that the company is not the only one with technology able to perform chromosomal array analysis of FFPE POC samples. (genomeweb.com)
They can be used for glycan profiling of various samples, including purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, or entire cells. (muni.cz)
Epidemiology of spotted fever group and typhus brain tissue samples from stillborn infants and from pla- group rickettsial infection in the Amazon basin of Peru. (cdc.gov)
Microvessel density, the number of proliferating vessels and the presence of glomeruloid proliferations (GMPs) were also assessed in tissue samples. (uib.no)
To identify genes that ASPP2 uses to suppress SCC, we will also conduct gene array analysis on SCC samples from ASPP2 exon3-null and heterozygous mice, and compare them with tissue samples from wild type mice. (ukri.org)
Results The spatial distributions of TANs and TAMs were correlated with each other in patient-derived ICC samples. (bmj.com)
42 pathologic cardiac tissue samples frequency and are aetiologic in cardiac malforma- were sequenced. (cdc.gov)
Abnormal samples can increase risk for poor data quality,15 we cardiac development appears to occur through a were interested in replicating these experiments process that is heterogeneous and complex, with using fresh frozen cardiac tissue instead of formalin both environmental and genetic risk factors.1 fixed tissue after decades of storage. (cdc.gov)
The samples were collected from breast cancer patients rent procedures. (lu.se)

NEW YORK (GenomeWeb) - In a study published today in Cancer Cell , researchers from South Korea, the Netherlands, and the US National Institutes of Health reported their new proteogenomic analysis of diffuse gastric cancers (GCs) in young populations, identifying four subtypes of the disease. (genomeweb.com)

Subsequently, the prognostic immune-related gene signature was successfully constructed from highly immune-related genes through COX regression and LASSO COX analysis. (techscience.com)

Many research and audit projects have already used these data, including the construction of a 274-case tissue microarray for renal cell carcinoma, microRNA hybridization arrays and analysis of 900 nephrectomy cases from the past three decades. (ox.ac.uk)
The most frequently detected abnormalities were hyperechoic rings around renal pyramids (28.3% of patients). (medscape.com)
These results suggest that the use of alum as an oral antiplatelet drug could be explored further, taking into account possible side-effects especially in renal compromised patients. (who.int)

Lung tissues of patients with RIPF and idiopathic pulmonary fibrosis, and lung tissues from various fibrotic mouse models, as well as appropriated cell line systems were used. (biomedcentral.com)
Inverse correlation between pHSP27 and Smurf2 was observed in the lung tissues of PF animals, an irradiated orthotropic lung cancer models, and PF tissues from patients. (biomedcentral.com)

To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
analyzing the velocity (i.e., the change over time) of the markers, malignancy we could stipulate for each patient the risk of developing metas- tasis after the end of adjuvant systemic therapy. (lu.se)

CONCLUSION: MMR assays identify patients with a low risk of recurrence. (ox.ac.uk)

The diagnostic value of hub genes was assessed by receiver operating characteristic analysis and validated in GSE1297. (nature.com)

Tumor cell proliferation by Ki-67 had significant prognostic impact by multivariate analysis. (biomedcentral.com)

In contrast, certain colors or wavelengths in the infrared (IR) and ultraviolet (UV) regions are absorbed strongly by biological tissues. (nationalacademies.org)
The glycosylation of cells reflects the biological species, tissue, and physiological state of the organism. (muni.cz)

[ 2 ] The clinician wishing to investigate identified abnormalities may use a scalpel biopsy or a punch biopsy to obtain a representative tissue sample for pathologic analysis. (medscape.com)

HER2+ Breast cancer (BC): HER2 overexpression in BC patients has been associated with poor response to hormone therapy and chemotherapy. (tmu.edu.tw)
The NC group consisted of 143 patients receiving only intravenous chemotherapy, while the E group consisted of 143 patients receiving intraperitoneal perfusion of fluorouracil and cisplatin combined with intravenous chemotherapy. (bvsalud.org)
Adjuvant chemotherapy with six cycles of mFOLFOX6 (leucovorin, 5-fluorouracil and oxaliplatin) will be used for patients with adverse pathological features. (bvsalud.org)
KRAS mutational analysis provides useful additional risk stratification to guide use of chemotherapy. (ox.ac.uk)
Tissue sections from chemotherapy-treated DA WTs (n = 12) were compared with chemotherapy-treated nonanaplastic WTs (n = 15) in a tissue microarray system, enabling analysis of 769 tumor regions. (lu.se)

But the TCGA approach, which was performed using reverse-phase protein arrays, is limited by antibody availability. (genomeweb.com)

Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays. (ox.ac.uk)
Nucleic acid programmable protein arrays constitute a powerful tool to study autoimmune diseases. (ox.ac.uk)

HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
Normal colon tissue from cancer patients displayed gains in chromosome 19 and 20. (lu.se)

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. (lookformedical.com)

Statistical analysis of these results will be done in the Biostatistics and Information Sciences core. (grantome.com)
Results: One non-synonymous germline sequence variant iants as well as multiple haplotypes were observed was identified in one patient. (cdc.gov)

A 1:1 matched case-control study was conducted, with the normal control (NC) group and experimental (E) group being composed of patients who underwent the corresponding treatment for primary GC with surgery within 2 months and the same pathological tumor-node-metastasis (pTNM) stage. (bvsalud.org)

Unsupervised hierarchical clustering of serum concentrations assessed by the multiplex array did not show any specific pattern. (uib.no)

Genomic analysis by transcriptome sequencing to predict the outcomes in HR patients. (tmu.edu.tw)
Its increased precision, leading to better treatment outcomes, greater patient comfort, and faster healing time, has been central to fostering stronger market acceptance. (reportlinker.com)
ORI consolidates multiple systems into a unified platform, improving process efficiency, and patient care outcomes. (reportlinker.com)
The outcomes of the second interim analysis included a resizing of the trial to 650 patients and the addition of an exclusion criterion of subjects with multiple organ dysfunction score (MODS) smaller than = 9. (apoptosisblog.com)
All three of those factors were independent predictors of patient outcomes. (bmj.com)

Autoantigens identified were subjected to Ingenuity Pathway Analysis to determine the patterns of signaling cascades or tissue origin. (ox.ac.uk)

In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757. (biomedcentral.com)
This organism produces a diverse array of virulence factors, including toxins, adhesins, colonization and biofilm factors. (biomedcentral.com)

We show that short-chain genotoxic alkanals and also medium-chain alkanals are specifically enriched in EAC and associated tissues, on a genetic background of reduced aldehyde detoxification. (nature.com)
Recognition of a single-gene disorder as causal for a patient's 'multiple sclerosis-like' phenotype is critically important for accurate direction of patient management, and evokes broader genetic counselling implications for affected families. (medscape.com)

Of the different tumour types arising in epithelial tissues, squamous cell carcinoma (SCC) is the most malignant and has the highest metastatic potential. (ukri.org)

Anatomy4

Organisms1

Diseases10

Chemicals and Drugs10

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes27

Disciplines and Occupations1

Information Science2

Health Care3

Geographicals1

Biopsies7

Prognosis13

Tumors9

Normal tissues3

Genomic analyses2

Clinical24

Organs6

Paraffin-embedded2

MRNA expression3

Microarrays8

Biomarkers4

Determined by immunohistochemistry1

Formalin-fixed1

Expression32

TMAs1

Cohort5

Expressed genes1

Diagnosis9

Methods11

Primary tumor2

Pathways6

Exome1

0.0011

Survival7

Epithelial cells2

Enrichment2

Proteins7

Immunohistochemical4

Molecular2

Quantitative2

Microarray analysis2

Significantly2

Predictive1

High-throughput1

Cohorts1

Samples14

Gastric1

Regression1

Renal3

Lung2

Markers2

Conclusion1

Receiver operating char1

Multivariate analysis1

Biological2

Pathologic1

Chemotherapy5

Antibody1

Protein arrays2

Colon2

Differential1

Results2

Underwent1

Multiplex1

Outcomes5

Pathway Analysis1

Factors2

Genetic2

Tumour1