• MicroRNAs, small RNA molecules that affect the stability of messages and the amount of proteins made by genes, can control many aspects of development and cell function and often are altered in diseases such as cancer. (hopkinsmedicine.org)
  • Lamin A/C proteins have key roles in nuclear structural integrity and chromosomal stability. (mdpi.com)
  • It emerges that semaphorin proteins guide the wiring of these pathways. (natureasia.com)
  • A second major area of interest focuses on how proteins contribute to RNA stability and degradation. (mskcc.org)
  • RNA, or ribonucleic acid, has numerous functions in the cell including the ability to convey genetic information in a form called messenger RNA, which is read by ribosomes to make proteins. (mskcc.org)
  • The pleiotropic effects of both proteins are perfect examples of the im​portance of RNA decay and translation in maintaining correct cell functioning. (ens-lyon.fr)
  • In addition, numerous important cellular proteins, such as B-cell lymphoma-2 homologous antagonist/killer, Myc proto-oncogene protein, cyclin-dependent kinase inhibitor 1B, DNA replication licensing factor MCM-7, retinoblastoma 1 and Annexin A1, are degenerated through the UBE3A-mediated ubiquitin proteasome pathway ( 7 ). (spandidos-publications.com)
  • In addition to continuing the experimental protein and RNA folding studies, new research areas in the Sosnick lab involve the rational design of proteins and RNAs to control their structure and function, and the expansion of the computational component. (openwetware.org)
  • In this project, I will employ biochemical, cell, systems biology and computational approaches to study the relevance of post-transcriptional regulators such as RNA-binding proteins and microRNAs in DDR. (europa.eu)
  • This will provide a global insight into the dynamics of RNAs and proteins during DDR. (europa.eu)
  • J Med Chem 2005) and extended it to macromolecular complexes involving proteins and protein-RNA complexes beyond topoisomerases (Pommier et al. (cancer.gov)
  • These biomarkers are detectable in liquid biopsy, include circulating tumor cells (CTCs), proteins, cell free DNA (cfDNA) and RNA (cfRNA) that recently have acquired interest in cancer for their role in diagnosis and treatment response [ 1 , 3 ]. (scientificarchives.com)
  • The proteins produced from the BRCA1 and BRCA2 genes are involved in fixing damaged DNA, which helps to maintain the stability of a cell's genetic information. (medlineplus.gov)
  • Non-coding RNA (ncRNA) such as microRNA (miRNA) and long non-coding RNA (lncRNA) regulates the translation and/or stability of multiple mRNA targets either directly or by modulation of signal transduction pathways. (frontiersin.org)
  • Signal transduction pathways regulate gene expression in part by modulating the stability of specific mRNAs. (ed.ac.uk)
  • systems biology of signal transduction pathways. (stanford.edu)
  • Since inhibitors of protein synthesis are known to activate cellular signal transduction pathways, our findings demonstrate new mechanisms for the regulation of HSV-1 IE gene expression which may be important during latency and reactivation. (microbiologyresearch.org)
  • Our long-term goal is to delineate the molecular bases and order of genetic, epigenetic and cellular processes that link altered expression of long ncRNAs and pathways driven by these molecules to cancer. (duke.edu)
  • Dr. Lima's laboratory uses structural, biochemical, and functional analyses of molecules involved in RNA processing and protein modification with a focus on understanding how these pathways contribute to cell growth and disease. (mskcc.org)
  • Work in the Lima lab has focused on how an RNA-degrading complex called the RNA exosome targets these RNA molecules for destruction. (mskcc.org)
  • The RNA exosome is composed of many protein subunits that assemble into a unique arrangement capable of binding and degrading targeted RNA molecules. (mskcc.org)
  • MicroRNA (miRNA) molecules, a series of single-stranded non-coding RNA chains measuring 20-25 nucleotides in length, regulate gene expression at the transcriptional level by complementary pairing with target gene mRNA ( 5 ). (spandidos-publications.com)
  • The over-all goal of my research program is to understand how complex biological molecules adopt their functional, 3-dimensional conformations: The Protein and RNA Folding Problem. (openwetware.org)
  • In addition, I will characterize the transcriptome-wide response of existing RNA molecules as well as the newly synthesized transcripts. (europa.eu)
  • Using a small noncoding RNA, microRNA 211, and tools that track the stability and decay of the protein-coding and noncoding RNAs in lab-grown melanoma cells, a team led by a Johns Hopkins Kimmel Cancer Center researcher identified highly unstable RNA molecules in human melanomas , including a novel miR-211 target gene DUSP3. (news-medical.net)
  • In the Yildirim lab, we study how epigenetic mechanisms, particularly those that are mediated by long noncoding RNAs (ncRNAs), complement gene expression, impact genome stability and define cell fate decisions. (duke.edu)
  • Our laboratory uses primarily XCI as a model to understand how long ncRNAs 1) regulate gene dosage and maintain epigenetic state, 2) impact genome stability, and 3) participate in cell fate decisions. (duke.edu)
  • To better apprehend how a RNA can act on gene expression regulation as part of a RiboNucleoProtein complex (RNP), we are also interested in specific RNP formations and processings. (ens-lyon.fr)
  • From the RNA standpoint, recent discoveries of the diverse roles structural RNAs play in gene expression, such as riboswitches, illustrate the importance of understanding their dynamics and folding as well. (openwetware.org)
  • To gain insights into the relationship between genotype, phenotype and disease, FMI scientists work to understand genome output by molecularly defining the control of gene expression and of RNA and protein stability. (fmi.ch)
  • RNA Interference (RNAi) technology has made it possible to effectively regulate gene expression. (altogenlabs.com)
  • Our findings demonstrate a direct link between a specific signal transduction pathway and a specific RNA-binding protein, both of which are known to regulate TNF-alpha gene expression at a posttranscriptional level. (ed.ac.uk)
  • The Martinez lab studies RNA regulatory mechanisms that control gene expression. (stanford.edu)
  • Furthermore, methylation sequencing results showed that LPSN caused massive gene methylation changes, which enriched in over 20 GO pathways in the filial overexpression line, and the expression of OsNAR2.1 in LPSN filial overexpression plants was significantly reduced compared to HPSN filial plants in high external N, which was not shown in wild type. (biomedcentral.com)
  • DNA methylation is the major heritable epigenetic modification and contributes to the epigenetic regulation of nuclear gene expression and genome stability [ 13 ]. (biomedcentral.com)
  • One gene, dual specificity phosphatase 3 (DUSP3), regulates important melanoma signaling pathways. (news-medical.net)
  • Tissue samples collected from the anterior glenohumeral joint capsule during surgery will undergo RNA-seq to determine differences in gene expression between the study groups. (biomedcentral.com)
  • In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)
  • As cellular metabolic pathways follow the law of mass action, a substantial number of miRNAs targeted to numerous viral mRNAs should be produced. (ldsfreedomforum.com)
  • Despite this, the mRNAs have concerns about their stability and immuno-stimulation, therefore, researchers and companies have concentrated their work to address this issue [2]. (researchgate.net)
  • Here, we show that human antigen R (HuR), an RNA binding protein which binds preferentially to AU-rich elements (AREs) and stabilizes mRNAs, regulates TNF-α-induced ICAM-1 expression in the MK2/HuR signaling pathway. (biomedcentral.com)
  • Considering that AREs located in the 3′-UTR of ICAM-1 and IL-8 mRNAs, we hypothesize that the MK2 pathway might regulate the expression of ICAM-1 and IL-8 through HuR in TNF-α-induced HPMECs. (biomedcentral.com)
  • Circulating RNAs are represented by a broad range of subtypes, such as microRNA (miRNAs), long non coding RNA (lncRNAs), messenger RNAs (mRNAs), transfer RNAs (tRNAs), snRNAs (small nuclear RNAs), snoRNA (small nucleolar RNAs), piwiRNAs (piRNA) and circRNAs (circular RNAs). (scientificarchives.com)
  • Circulating RNAs are represented by, such as for intercellular RNAs, a broad range of subtypes, mainly represented by microRNA (miRNAs), long non coding RNA (lncRNAs) and messanger RNAs (mRNAs). (scientificarchives.com)
  • Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). (mdpi.com)
  • To conclude, it was identified that the probable anti‑CA mechanism of miRNA‑375 modulates HPV through the UBE3A and IGF‑1R pathway in cervical cancer cells. (spandidos-publications.com)
  • The expression of genes through miRNA is regulated by endogenous regulatory pathways, resulting in high stability and biocompatibility ( 5 ). (spandidos-publications.com)
  • It is also possible that the host's miRNA machinery might be overwhelmed in the processing of miRNAs and diverted away from its normal cellular functions and molecular pathways. (ldsfreedomforum.com)
  • This might lead to dangerous, long-lasting dysregulation of the miRNA pathways. (ldsfreedomforum.com)
  • It is reasonable to assume that some viral messenger RNAs will trigger changes in the host miRNA transcription profiles or stabilities and that the resulting modified miRNA clusters might favor the development of different disorders. (ldsfreedomforum.com)
  • In endometrial cancer only some of these cf-RNAs have been investigated, no studies have been performed on snoRNAs, piRNAs, snRNAs and tRNA, the most analyzed one is represented by cf-miRNA in plasma. (scientificarchives.com)
  • These findings open an immediate use of selected cf-miRNA as biomarker in liquid biopsy of EC and suggest further investigation of the other cf-RNAs to implement the knowledge in this fascinating field. (scientificarchives.com)
  • Even though the role of circulating non coding RNAs as miRNA and lncRNA can be easily understood taking into consideration that can be secreted and persist in biofluid in remarkably stable forms, as "free" or associated to exosomes, less is known concerning the role of circulating mRNA, mainly taking into consideration its instability and the presence of RNase, nevertheless its presence is detected in blood. (scientificarchives.com)
  • The quality and quantity changes of circulating RNAs, mainly miRNA, are broadly investigated and associated with the initiation and progression of cancer [ 5 ]. (scientificarchives.com)
  • However, for an immediate and effective response, global post-transcriptional control of mRNA stability, splicing and translation may be crucial during "immediate-to-early" DDR. (europa.eu)
  • N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)
  • MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. (mdpi.com)
  • It is also known that most of the miRNAs that operate in the nucleus will simultaneously regulate transcript stability in the cytoplasm and vice versa, resulting in a highly integrated mechanism. (ldsfreedomforum.com)
  • In response to stimuli, the cell carefully regulates the abundance of certain messenger RNA by balancing the synthesis of new RNA against the destruction of old RNA once it has fulfilled its function. (mskcc.org)
  • The zinc finger protein tristetraprolin (TTP) is expressed in response to LPS and regulates the stability of TNF-alpha mRNA. (ed.ac.uk)
  • Noncoding RNAs (ncRNAs) are noncoding transcripts that take part in epigenetic mechanisms by providing RNA-directed silencing, aiding recruitment of chromatin modifying complexes and in some instances, presenting enhancer-like functions to boost transcription. (duke.edu)
  • We found that species-specific pausing during RNA transcription improves the folding behavior, thereby suggesting that RNA sequences have co-evolved with the pausing properties of their cognate RNA polymerases. (openwetware.org)
  • DHX9 is an RNA/DNA helicase that is involved in multiple cellular processes, including transcription and maintaining genome stability. (gla.ac.uk)
  • the interface between RNA processing and transcription with genome stability. (stanford.edu)
  • In addition, our study showed that TGF-β activated LINC01980 transcription through the canonical TGF-β/SMAD signaling pathway in HCC. (researchsquare.com)
  • Other researchers, Perera notes, have attempted to profile genomewide transcript stability by using chemicals that block all transcription, and then observing the stability of the transcripts that existed before blocking. (news-medical.net)
  • In this study, we used real-time reverse transcription-PCR (RT-PCR) to measure levels of shed viral RNA in saliva, urine, and feces of subcutaneously inoculated bank voles until they were killed at day 133 postinfection (PI). (cdc.gov)
  • Regulation of RNA stability and translation are two key steps of cellular homeostasis. (ens-lyon.fr)
  • Besides its role in the regulation of genes, DNA methylation silences repetitive elements and appears to be important for the stability of the mammalian genome. (caister.com)
  • The Cdk5-AMT signal pathway involved in regulation of DDR by TIGAR defines a new role of TIGAR in cancer cell survival and it suggests that TIGAR may be a therapeutic target for cancers. (nature.com)
  • Protocol.Regulation of lymphokine messenger RNA stability by a surface-mediated T cell activation pathway. (iassist2012.org)
  • Discussing the mechanisms of UTR regulation, the role of genetic variants in modulating RNA processing, and protein production in human disease. (advancedsciencenews.com)
  • In the past, we have been working on two cellular factors, the translation initiation factor INT6/EIF3E and the RNA helicase UPF1, that act at the crossroads of RNA translation and decay. (ens-lyon.fr)
  • In addition to the RNA helicase UPF1, we develop an axis of research based on the G4 resolvase DHX36 (project 2) . (ens-lyon.fr)
  • To explore the influence of the long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on the osteogenic differentiation in bone marrow mesenchymal stem cells. (ijpsonline.com)
  • 2019) Transcriptome stability profiling using 5'-bromouridine IP chase (BRIC-seq) identifies novel and functional microRNA targets in human melanoma cells. (news-medical.net)
  • Mendell's team was one of the first to identify microRNAs that are involved in cancer-promoting molecular pathways. (hopkinsmedicine.org)
  • Alteration of these regulatory mechanisms can modify molecular pathways and cellular processes, thus affecting phenotype, disease onset, and possibly even disease outcome. (advancedsciencenews.com)
  • Mauno Vihinen is well-known for his experience and interest in investigating variations and their effects whether they emerge at molecular levels (DNA, RNA protein), in structural context or in the cellular networks and pathways. (lu.se)
  • Revealing how molecular interactions are optimized to control both oligomerization state and assembly pathway in protein assemblies is of vital importance for our understanding of biomedically important biological assemblies, as well as for efforts to manipulate them. (lu.se)
  • Messenger RNA (mRNA) vaccines represent a relatively new vaccine class showing great promise for the future. (researchgate.net)
  • Levels of shed viral RNA peaked within 11-28, 14-21, and 11-28 days postinfection for saliva, urine, and feces, respectively. (cdc.gov)
  • In contrast, blood of 5 of 6 animals contained viral RNA at day 133 postinfection, suggesting that bank voles secrete virus only during a limited time of the infection. (cdc.gov)
  • To evaluate possible transmission routes for PUUV, we investigated infectivity of different excretions and used a subset of viral RNA-positive saliva, urine, and feces samples to intranasally inoculate virus-negative bank voles. (cdc.gov)
  • 1. Alternating 2'-F bases and 2'OMe bases in siRNA enhances duplex stability and are more resistant to RNase degradation. (genelink.com)
  • Polyadenylation of eukaryotic transcripts is usually restricted to mRNA, providing transcripts with stability from degradation by nucleases. (syr.edu)
  • Conversely, an RNA degradation pathway can be signaled through poly (A) tailing in prokaryotic, archeal, and organellar biology. (syr.edu)
  • The functions of polyadenylation of rRNA in this organism are largely unknown at present, but the presence of internal polyadenylation sites, along with the presence of truncated segments of the rRNA, may suggest a role of the polyadenylation in the degradation pathway, a function typical of prokaryotes, archaea, and organelles. (syr.edu)
  • Dyskenin is composed of 514 amino acids and has a role in ribosomal RNA processing and telomere maintenance. (medscape.com)
  • Most of the m 6 A RNA methylation regulators were confirmed to be differentially expressed among groups stratified by clinical characteristics and tissues. (hindawi.com)
  • We identified an m 6 A RNA methylation regulator-based signature (including METTL3, YTHDC2, and YTHDF2) that could effectively stratify a high-risk subset of these patients by univariate and LASSO Cox regression, and receiver operating characteristic (ROC) analysis indicated that the signature had a powerful predictive ability. (hindawi.com)
  • In summary, we demonstrated the vital role of m 6 A RNA methylation regulators in the initial presentation and progression of HCC and constructed a nomogram which would predict the clinical outcome and provide a basis for individualized therapy. (hindawi.com)
  • Sequences of the DNA and RNA of 500 human cancers that have spread from their primary site in the body take us a step closer to the convergence of basic science and patient benefit. (natureasia.com)
  • TBL1XR1 over-expression has been linked to poor prognosis in several solid organ cancers, impacting various oncogenic signaling pathways ( 6 ). (iiarjournals.org)
  • Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, and N6-methyladenosine (m 6 A) is a predominant internal modification of RNA in various cancers. (hindawi.com)
  • The resulting quantitative understanding is critical to comprehend impact and diagnostic potential of genetic variation for personalized health and to explore novel pathways for intervention. (fmi.ch)
  • When a disruption in this Notch pathway occurs, either by chemical or genetic means, it causes developmental malformations. (frontiersin.org)
  • Global nOPV2 genomic surveillance circulating vaccine-derived polioviruses (cVDPVs) across mul- during March-October 2021 confirmed genetic stability of tiple countries in Africa and Asia in recent years pose a major the primary attenuating site. (cdc.gov)
  • So we decided to look at a completely new angle of genomewide RNA stability and decay during the first steps in the development of melanoma. (news-medical.net)
  • Other classes of non-coding RNAs are likely to be found with new regulatory roles. (openwetware.org)
  • We focus on mRNA processing, RNA modifications and their roles in development and disease. (stanford.edu)
  • UBE3A is associated with cervical cancer and may combine with the E6 proto-oncogene encoded by HPV16 within cervical cancer cells to form the E6/E6-AP protein complex through the ubiquitin proteasome pathway ( 4 ). (spandidos-publications.com)
  • The RAS-regulated RAF-MEK1/2-ERK1/2 signalling pathway is frequently de-regulated in human cancer. (babraham.ac.uk)
  • Previous work by other lab groups have demonstrated that DHX9 is linked to multiple signalling pathways that are involved in cancer development, such as the mTOR and p53 pathway. (gla.ac.uk)
  • Malentacchi F, Sorbi F, Cipriani N, Sgromo C, Antonuzzo L, Pillozzi S. Circulating Cell-Free RNA: A New Perspective for Endometrial Cancer. (scientificarchives.com)
  • Recently, circulating RNAs (cfRNAs) are increasing their importance as biomarkers in liquid biopsy for cancer patients both for tumor characterization and development and for setting a monitoring personalized therapy. (scientificarchives.com)
  • 1. 2'- OH is not required for siRNA to enter the RNAi pathway. (genelink.com)
  • 4. Modified siRNAs enter into the RNAi pathway in vitro. (genelink.com)
  • The ability of RNAi to knockdown target genes enables scientists in identification and validation of drug targets as well as in the discovery of cell-signaling pathways that are vital to organism survival and disease control. (altogenlabs.com)
  • The negative prognosis associated with PARP1 and POLD2 expression along with the apparent melphalan-sensitizing effect of PARP inhibition may suggest this pathway as a potential biomarker in patients with MM in the setting of ASCT. (haematologica.org)
  • This suggests that DNA replication following escape from G1 arrest in drug is more error prone and provides a potential explanation for the DNA damage observed under long-term RAF-MEK-ERK1/2 pathway inhibition. (babraham.ac.uk)
  • EUL) pathway in November 2020, allowing for its first use mutations in domain V, no recombination, and 0-5 VP1 substi- for outbreak response in March 2021 ( 6 ). (cdc.gov)
  • In the autosomal dominant form, mutations in the RNA component of telomerase ( TERC ) or telomerase reverse transcriptase ( TERT ) are responsible for disease phenotype. (medscape.com)
  • Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
  • DAVID pathway analysis revealed an enrichment of cellular pathways involved in cytoskeletal and cilia processes among the set of common genes (142 genes) perturbed by both diacetyl and 2,3-pentanedione. (nature.com)
  • Across 450 clinical samples and six disease stages, expression levels of genes in the BER pathway were found to be highly upregulated during the development of MM. In a separate cohort of 559 patients with MM treated with ASCT, expression of BER pathway members MPG and PARP3 was positively associated with overall survival (OS) while expression of PARP1, POLD1, and POLD2 was negatively associated with OS. (haematologica.org)
  • Materials and Methods: Digital RNA quantification (Nanostring) of previously curated 188 B-cell lymphoma specimens across four subtypes, follicular lymphoma (FL), diffuse large B-cell lymphoma, not otherwise specified (DLBCL-NOS), primary testicular lymphoma (PTL), and plasmablastic lymphoma (PBL), was reanalyzed with focus on TBL1XR1 and NCOR1 expression, juxtaposing them with 730 ontogenically linked genes. (iiarjournals.org)
  • These genes collaborate in a complicated pathway (FA pathway), which is responsible for the repair of DNA damage. (medscape.com)
  • For example, the mitogen-activated protein kinase (MAPK) p38 pathway mediates stabilization of tumor necrosis factor alpha (TNF-alpha) mRNA in myeloid cells stimulated with bacterial lipopolysaccharide (LPS). (ed.ac.uk)
  • Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
  • Ingenuity® Pathway Analysis was used to identify significantly affected pathways in the transcriptomic and proteomic data sets. (biomedcentral.com)
  • Here we interrogated the role of the base excision DNA repair (BER) pathway in MM response to ASCT. (haematologica.org)
  • Melphalan acts by alkylating DNA and causing single-strand DNA breaks as well as other DNA lesions, primarily repaired through the base excision repair (BER) pathway. (haematologica.org)
  • 2018). While studying the tyrosyl-DNA-phosphodiesterase (TDP1 and TDP2) repair pathways for the excision of topoisomerases from DNA, Dr. Pommier revealed that TDP1 repairs a broad range of 3'-blocking lesions in addition to TOP1 (Murai et al. (cancer.gov)
  • This sledgehammer method, however, is considered toxic, and can alter other aspects of a cell's biology in ways that may obscure transcript stability information. (news-medical.net)
  • The 3',5'-cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) are known to play a role in disease progression as they mediate downstream signalling pathways that can promote cell growth and disease progression. (gla.ac.uk)
  • again, risk of progression to AIDS, as determined by HIV RNA viremia and CD4+ T cell count, should guide the decision to treat. (cdc.gov)
  • MK2 were subjected to a knockdown by interfering RNA, the mRNA and protein levels of HuR in human pulmonary microvascular endothelial cells (HPMECs) were not affected. (biomedcentral.com)
  • To address this, Perera and colleagues used BRIC-seq, a method that tags transcripts with 5-Bromouridine, which is incorporated into newly synthesized RNAs and then can be immunocytochemically analyzed over time without disturbing the rest of the cellular environment. (news-medical.net)
  • This work opens up a whole new avenue of structural studies on non-coding RNAs. (openwetware.org)
  • Our long-term goal is to create residue-level structural models using the cryoEM images, sequence information, and all-atom simulations, building on our modeling studies of RNA folding intermediates. (openwetware.org)
  • In a melanoma cell line that normally lacks miR-211, the researchers introduced miR-211 expression and then used the BRIC-seq method to investigate transcriptome stability dynamics in the presence/absence of miR-211. (news-medical.net)
  • However recent works clearly demonstrate that RNA stability per-se is critical for cell homeostasis. (ens-lyon.fr)
  • These fundamental studies have helped us examine how non-coding RNAs may fold in the cell. (openwetware.org)
  • Previous study revealed that the protective effect of TIGAR in cell survival is mediated through the increase in PPP (pentose phosphate pathway) flux. (nature.com)
  • SmartBase TM modifications go beyond the traditional use of RNA bases for constructing synthetic siRNAs to specifically increase duplex stability, nuclease resistance and cell permeation. (genelink.com)
  • Notch signaling is highly conserved cell signaling pathway, which is involved in diverse embryonic organs or tissue development as well as regeneration ( 1 - 10 ). (frontiersin.org)
  • His group has developed a method for revealing networks from experimental data and investigated with systematic computational perturbations effects of T-cell primary immunodeficiencies on the central signaling pathways. (lu.se)
  • however, the strength of the recommendation to treat should be based on the patient's willingness to accept therapy as well as the prognosis for AIDS-free survival as determined by the HIV RNA copy per mL of plasma and the CD4+ T cell count. (cdc.gov)
  • In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
  • At this time it is possible that mRNA and adenoviral DNA vaccines could influence key miRNA's in the causal pathway to a primary arrhythmic cardiac death in the absence of clinical myocarditis. (ldsfreedomforum.com)
  • 6. Active infection requiring therapy, including known positive tests for Hepatitis B surface antigen and hepatitis C virus (HCV) RNA. (who.int)
  • Thermal stability assays show that ERKi do not destabilise ERK2 (or ERK1) in vitro, suggesting that ERK2 turnover is a cellular consequence of ERKi binding. (babraham.ac.uk)
  • Tests grams are using high-throughput in vitro screens for assessing chemical effects across multiple cellular pathways. (cdc.gov)
  • The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput "omics" approaches. (europa.eu)
  • However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
  • These studies ultimately led the researchers to angiogenesis-related pathways that "all converged" onto the angiogenesis-promoting cytokines interleukin-8 and CXCL1, he said. (genomeweb.com)
  • Transducin β-like 1 X-linked receptor 1 (TBL1XR1) and nuclear receptor corepressor 1 (NCOR1) are instrumental within the GC, modulating myriad oncogenic pathways. (iiarjournals.org)
  • The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
  • However, how long ncRNA-mediated pathways may impact tumorigenesis is still poorly understood. (duke.edu)
  • In combination with this work, the scientists were performing extensive bioinformatics analyses to identify the pathways regulated by the different miR-200 family members, which include miR-141, miR-200a, miR-200b, miR-200c, and miR-429, Sood explained. (genomeweb.com)
  • They reported their findings June 16 in RNA Biology online. (news-medical.net)
  • The same reasons that make amyloids lethal in biology make them useful as biomaterials (such as their superior stability). (lu.se)