• This short review provides a current synopsis of caudal fin regeneration in zebrafish with an emphasis on the molecular signaling networks that dictate epimorphic regeneration. (karger.com)
  • Adult zebrafish fins fully regenerate after resection, providing a highly accessible and remarkable vertebrate model of organ regeneration. (bvsalud.org)
  • Moreover, a dominant-negative approach in chick and knockdown analysis with morpholinos in zebrafish revealed their requirement for Fgf8 expression and limb outgrowth, and further indicate that they have a coordinated action on Fgf8 expression. (biologists.com)
  • Our study demonstrates that Sp8 and Sp9 , via Fgf8 , are involved in mediating the actions of Fgf10 and Wnt/β-catenin signaling during vertebrate limb outgrowth. (biologists.com)
  • At the outset, the fundamentals of caudal fin architecture and the stages of epimorphic regeneration are described. (karger.com)
  • This is followed by a detailed look at the main networks implicated in fin regeneration, namely the Wnt, fibroblast growth factor, activin-βA, retinoic acid and hedgehog signaling pathways. (karger.com)
  • Throughout this mini-review, these molecular networks are examined through the lens of wound healing, blastema formation or regenerative outgrowth, three of the main stages of epimorphic regeneration. (karger.com)
  • Next, the emerging role of noncoding RNAs as regulators of regeneration and mechanisms of regenerative termination are discussed. (karger.com)
  • Therefore, such methods are mainstays of comprehensive, hypothesis-driven fin regeneration studies. (bvsalud.org)
  • Aryl hydrocarbon receptor activation impairs extracellular matrix remodeling during zebra fish fin regeneration. (oregonstate.edu)
  • Proper regulation of transcription, innervation, vascularization, and extracellular matrix (ECM) composition is essential for complete fin regeneration. (oregonstate.edu)
  • To investigate whether TCDD blocks regeneration by impairing ECM remodeling, male zebra fish were i.p. injected with 50 ng/g TCDD or vehicle, and caudal fins were amputated. (oregonstate.edu)
  • Understanding the molecular mechanisms that control how outgrowth of different tissues and organs of the embryo are established and maintained is one of the major questions in the study of embryonic development. (biologists.com)
  • Balanced cell proliferation and redifferentiation along with cell movements then progressively reestablish patterned tissues and restore the fin to its original size and shape. (bvsalud.org)
  • Antibody-based staining of sectioned fins facilitates such high-resolution analyses of specific, native proteins. (bvsalud.org)
  • Adult zebra fish completely regenerate their caudal (tail) fin following partial amputation. (oregonstate.edu)
  • Quantitative real-time PCR studies revealed that the aryl hydrocarbon pathway is active and that matrix-remodeling genes are expressed in the regenerate following TCDD exposure. (oregonstate.edu)
  • Fin injury triggers wound epidermis formation and the dedifferentiation of injury-adjacent mature cells to establish an organized blastema of progenitor cells. (bvsalud.org)
  • We show that two closely related buttonhead -like zinc-finger transcription factors, Sp8 and Sp9 , are expressed in the AER, and regulate Fgf8 expression and limb outgrowth. (biologists.com)
  • While mature females are larger than males, pelvic fin growth and development is faster in males, suggesting a selective advantage to larger fins with faster development. (bvsalud.org)
  • Future work could examine pathways through which HPgV influences HIV control, which might inform the development of new therapeutics. (nih.gov)
  • First, for many developmental regulatory systems there is no convincing evidence that it is specific morphogenetic pathways per se that are conserved, rather than cell-type specification and differentiation processes. (silverchair.com)
  • Finally, the implications for future research and the broader field of regenerative medicine are examined. (karger.com)
  • Mature lepidotrichial-forming cells (fin ray-forming cells) were not observed in the fins of TCDD-treated fish. (oregonstate.edu)
  • Here, we present detailed, multistep procedures for antibody staining and co-detecting proliferating cells using paraffin and frozen fin sections. (bvsalud.org)
  • Alternatively, they could represent the only example of an independent third set of paired appendages in an extant taxon, if they articulate independently from any pelvic fin basal cartilages, challenging the current paradigm that extant jawed vertebrates are constrained to two sets of paired appendages. (bvsalud.org)