• Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
  • Gallic acid is formed from 3-dehydroshikimate by the action of the enzyme shikimate dehydrogenase to produce 3,5-didehydroshikimate. (wikipedia.org)
  • The enzyme contained 2 g-atoms of iron per mol of protein. (shengsci.com)
  • Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
  • PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
  • 2021. Differential roles of three different upper pathway meta ring cleavage product hydrolases in the degradation of dibenzo-p-dioxin and dibenzofuran by Sphingomonas wittichii strain RW1. (nih.gov)
  • 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
  • Dehydrogenation of phenanthrene-cis-9,10-dihydrodiol to produce the corresponding diol, followed by ortho-cleavage of the oxygenated ring, produced 2,2'-diphenic acid. (afpm.org.my)
  • nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
  • The identification of 2,2'-diphenic acid in culture extracts indicates that phenanthrene was initially attacked through dioxigenation at C9 and C10 to give cis-9,10-dihydrodiol. (afpm.org.my)
  • New metabolic pathways of phenanthrene and a better understanding of the phenoloxidases and dioxygenase mechanism involved in degradation of phenanthrene were explored in this research. (afpm.org.my)
  • Identification of metabolites from phenanthrene oxidation by phenoloxidases and dioxygenases of Polyporus sp. (afpm.org.my)
  • Gallic acid (also known as 3,4,5-trihydroxybenzoic acid) is a trihydroxybenzoic acid with the formula C6H2(OH)3CO2H. (wikipedia.org)
  • Heating gallic acid gives pyrogallol (1,2,3-trihydroxybenzene). (wikipedia.org)
  • Gallic acid is found in a number of land plants, such as the parasitic plant Cynomorium coccineum, the aquatic plant Myriophyllum spicatum, and the blue-green alga Microcystis aeruginosa. (wikipedia.org)
  • Benzoic acid Catechol Hydrolyzable tannin Pyrogallol Syringol Syringaldehyde Syringic acid Shikimic acid Haslam, E. (wikipedia.org)
  • This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. (afpm.org.my)
  • low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
  • We examined kinetic parameters (K(m) and Vmax) for catechol 2,3-dioxygenase (C230), a key shared enzyme of the toluene-degradative pathway for these strains, and compared these parameters with those for the analogous enzymes from archetypal toluene-degrading pseudomonads which did not show enhanced, nitrate-dependent toluene degradation. (nih.gov)
  • Moreover, deletion analysis of the nucleotide sequence upstream of the translational start of the meta-pathway operon that contains tbuE, the gene that encodes the C230 of strain PKO1, allowed identification of sequences critical for regulated expression of tbuE, including a sequence homologous to the ANR-binding site of Pseudomonas aeruginosa PAO. (nih.gov)
  • Finally, the results of induction experiment indicated that thecatechol dioxygenases, both catechol 1,2-dioxygenase (C120) and catechol 2,3-dioxygenase (C230),were all inducible. (besjournal.com)
  • 13 Pseudomonas stutzeri's relative abundance was seen as a result of its ability to utilize aromatic hydrocarbons such as xylene, toluene, phenol, as well as polycyclic aromatic hydrocarbons , such as naphthalene . (kenyon.edu)
  • These enzymes include: benzoate dioxygenase, toluene 1,2-dioxygenase, and catechol 1,2-dioxygenase, which play a role in aromatic hydrocarbon degradation, and methanol dehydrogenase, which is involved in aliphatic hydrocarbon degradation. (kenyon.edu)
  • As far as catabolic functions, the clc element harbored, in addition to the genes for chlorocatechol degradation, a complete functional operon for 2-aminophenol degradation and genes for a putative aromatic compound transport protein and for a multicomponent aromatic ring dioxygenase similar to anthranilate hydroxylase. (nih.gov)
  • Advanced oxidation processes (APOs) of aromatic compounds, particularly of phenol, yield several benzene derivatives, such as hydroquinone, catechol, and resorcinol, as intermediate metabolites of its transformation. (hindawi.com)
  • Results Bacterial strains ZD 4-1 and ZD 4-3 were identified asComamonas testosteroni and Pseudomonas aeruginosa by 16S rDNA sequence analysis, respectively.The growth of the two strains was observed on a variety of aromatic hydrocarbons. (besjournal.com)
  • Analysis of the nucleotide and deduced amino acid sequences of C23O from strain PKO1 suggests that this extradiol dioxygenase belongs to a new cluster within the subfamily of C23Os that preferentially cleave monocyclic substrates. (nih.gov)
  • Conclusion The strains ZD 4-1 and ZD 4-3 metabolize phenol throughortho-pathways and meta-pathway, respectively. (besjournal.com)
  • Objective To investigate the characteristic and biochemical mechanism about the phenolbiodegradation by bacterial strains ZD 4-1 and ZD 4-3. (besjournal.com)
  • Methods Bacterial strains ZD 4-1 and ZD4-3 were isolated by using phenol as the sole source of carbon and energy, and identified by 16SrDNA sequence analysis. (besjournal.com)
  • The strains ZD 4-1and ZD 4-3 metabolized phenol via ortho-pathways and meta-pathways, respectively. (besjournal.com)
  • Species in the bacterial genus Pseudomonas present high potential for hydrocarbon degradation due to their metabolic diversity, their abundance in microbial communities, and their resistance to chemical remediation agents present at contamination sites. (kenyon.edu)
  • Pseudomonas abundance dropped off after a year, at t = 1y, after prolonged degradation of available hydrocarbons. (kenyon.edu)
  • In order to improve our knowledge of the microbial paracetamol degradation pathway, we inoculated a bioreactor with sludge of a hospital WWTP (Pharmafilter, Delft, NL) and fed it with paracetamol as the sole carbon source. (biorxiv.org)
  • Meanwhile, in the oxidative degradation of hydroquinone under a supercritical condition (409.9°C and 24.5 MPa) and subcritical condition (359.9°C and 24.5 MPa), -benzoquinone was to be an important intermediate [ 3 ]. (hindawi.com)
  • The formation of hydroquinone and -benzoquinone at early stages of phenol oxidation increases the toxicity of phenol wastewaters, showing that these compounds were more toxic and less degradable than the original pollutant [ 2 ]. (hindawi.com)
  • Characterization of Phenol Biodegradation by Comamonas testosteroni ZD4-1 and Pseudomonas aeruginosa ZD4-3[J]. Biomedical and Environmental Sciences, 2003, 16(2): 163-172. (besjournal.com)
  • Consistent with its relative abundance, researchers confirmed with PCR that Pseudomonas was actively expressing alkane hydroxylase genes and was responsible for up to 20% of total gene expression. (kenyon.edu)
  • The genes for catabolic functions were inducible under various conditions, suggesting a network of catabolic pathway induction. (nih.gov)
  • 12 Microbial community differentiation was observed in the Pseudomonas genus in response to oil contamination. (kenyon.edu)
  • Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. (nih.gov)
  • 3 There are currently 218 species assigned to Pseudomonas and the genus has considerable heterogeneity. (kenyon.edu)
  • The metabolic versatility of the genus allows Pseudomonas species to inhabit a wide variety of environments. (kenyon.edu)
  • 10 Pseudomonas species have been found to dominate hydrocarbon degrading bacterial communities even in the Artic-an environment that entails extreme temperature conditions, limited availability of nutrients such as nitrogen, and low levels of available water. (kenyon.edu)
  • b) Relative abundance of Pseudomonas species. (kenyon.edu)
  • Pseudomonas species comprised the bulk of the class Gammoproteobacteria . (kenyon.edu)
  • Metagenomic sequencing revealed that the dominance of Gammoproteobacteria was mainly due to Pseudomonas species (Figure 1B). (kenyon.edu)
  • Pseudomonas has shown robust response to oil contamination both in vitro and in situ . (kenyon.edu)
  • A 2013 study found that Pseudomonas was able to respond to new oil contamination by increasing its abundance and changing its community structure. (kenyon.edu)
  • In these mainly marine organisms, above a certain population density, light is produced continuously by the action of bacterial luciferase (LuxAB), which oxidizes a long-chain aldehyde such as tetradecanal in the presence of FMNH 2 and oxygen. (nih.gov)
  • 13094180 meta-cleavage compound hydrolase [Pseudomonas sp. (inrae.fr)
  • Gallic acid is found in a number of land plants, such as the parasitic plant Cynomorium coccineum, the aquatic plant Myriophyllum spicatum, and the blue-green alga Microcystis aeruginosa. (wikipedia.org)
  • 2 There is a great need for efficient and ecologically friendly remediation strategies. (kenyon.edu)
  • The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element, which normally resides at two locations in the cell's chromosome. (nih.gov)