Pathway degradation of aromatic catechol 2 3 dioxygenase. Medical search. Frequent questions

These bacteria subsequently employ 1,2-CTD in the last step of the degradation of aromatic compounds to aliphatic products. (wikipedia.org)
low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. (lbl.gov)
Microbial degradation of organic compounds. (research.com)
Benzene compounds with dihydroxylated rings, e.g. catechol (1,2-benzenediol) and hydroquinone (1,4-benzenediol), are important intermediates in bacterial catabolism of aromatic compounds. (ethz.ch)
They play a key role in the metabolism of organic compounds by increasing their reactivity or water solubility or bringing about cleavage of the aromatic ring. (biomedcentral.com)
Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)
A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)

The second hypothesis postulates that the tunnel regulates lipid membrane rigidity through its degradation of phenolic hydrocarbons and ability to bind to other lipids. (wikipedia.org)
1,2-CTD degrades phenolic hydrocarbons key to the synthesis lipid membranes. (wikipedia.org)
Therefore, 1,2-CTD may bind to the cell lipid membrane via its terminal phospholipids and thus have greater access to the phenolic hydrocarbons vital in lipid membrane structure. (wikipedia.org)
Polycyclic aromatic hydrocarbons (PAHs) degrading bacteria can be isolated from polluted coastal environments. (omicsonline.org)
Polycyclic aromatic hydrocarbons (PAHs) are one class of organic pollutants that are considered the most hazardous due to their toxic, mutagenic, and carcinogenic properties [ 1 ]. (omicsonline.org)
The analysis of both the metagenome of the consortium and the reconstructed metagenome-assembled genomes show that the most abundant bacterial genus in the consortium, Acidocella , possess many of the genes required for the degradation of diesel fuel aromatic hydrocarbons, which are often the most toxic component. (springeropen.com)

Thus far, 1,2-CTD has been observed to exist in the following species of soil bacteria and fungi: Pseudomonas sp. (wikipedia.org)
the 1,2-CTD enzyme produced by Pseudomonas arvilla is the exception to this rule, containing two highly homologous subunits that can form either a homo- or hetero- dimer. (wikipedia.org)
His primary areas of investigation include Organic chemistry, Toluene dioxygenase, Pseudomonas putida, Biochemistry and NAD+ kinase. (research.com)
The concepts of his Pseudomonas putida study are interwoven with issues in Escherichia coli, Electron donor, Dioxygenase, Dehydrogenase and Naphthalene. (research.com)
His Pseudomonas putida research is multidisciplinary, incorporating elements of Nuclear chemistry, Escherichia coli, Toluene, Chromatography and Toluene dioxygenase. (research.com)
His Escherichia coli research integrates issues from Regioselectivity, Microorganism, Pseudomonas putida, Metabolite and Toluene dioxygenase. (research.com)
They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
Alkaline cyanide degradation by Pseudomonas pseudoalcaligenes CECT5344 in a batch reactor. (paperbase.net)
Metabolism of 2,4,6-trinitrotoluene by Pseudomonas sp. (paperbase.net)

PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
Plant-mediated effects on polycyclic aromatic hydrocarbon (PAH) degradation by bacteria in the rhizosphere of the salt marsh grasses, Spartina alterniflora and Phragmites australis. (nih.gov)
Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)

Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
Dienelactone hydrolase (DLH), an enzyme from the beta-ketoadipate pathway, catalyzes the hydrolysis of dienelactone to maleylacetate. (inrae.fr)
An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. (lookformedical.com)

Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link ), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (lbl.gov)
The downstream transformation of BA involved a meta -cleavage pathway, although its genetic organization and function was not revealed and the overall network of genes driving the full metabolic pathway of OPP is still not known. (nature.com)
In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)

2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)

In MetaCyc pathway catechol degradation III (ortho-cleavage, link ), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate. (lbl.gov)
This series of steps is part of protocatechuate para-cleavage, link , or catechol degradation II, link . (lbl.gov)
2021. Differential roles of three different upper pathway meta ring cleavage product hydrolases in the degradation of dibenzo-p-dioxin and dibenzofuran by Sphingomonas wittichii strain RW1. (nih.gov)
2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. (inrae.fr)

2003. Characterization of a novel Acinetobacter species containing a toluene dioxygenase. (nih.gov)
Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)

Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
Pathway examination We've got made use of Ingenuity Pathway Examination to de CX-4945 clinical trial cipher the attainable biological relevance of gene expres sion improvements established. (pdpksignaling.com)
Gene sets established both by in silico examination of mRNA expres sion, GSEA of microRNA and comparative genomic hybridization gene sets have been subjected to IPA and considerable pathways were in contrast to each other. (pdpksignaling.com)
Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)

The two enzymes were identified to be a part of two separate catechol dioxygenase families: 1,2-CTD was classified as an intradiol dioxygenase while 2,3-CTD was classified as an extradiol dioxygenase. (wikipedia.org)

Crystal structure of naphthalene dioxygenase: side-on binding of dioxygen to iron. (research.com)
An integrated approach of bioassay and molecular docking to study the dihydroxylation mechanism of pyrene by naphthalene dioxygenase in Rhodococcus sp. (brenda-enzymes.org)

Based on the complexity of the degradation pathways, the phenomenon of biodegradation is categorized into two types: convergent and divergent modes of degradation (Fig. 1 ). (biomedcentral.com)

benK encodes a hydrophobic permease-like protein involved in benzoate degradation by Acinetobacter sp. (microbiologyresearch.org)

Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)

However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
Therefore, elucidation of the microbial metabolic pathway of OPP is a prerequisite for the downstream exploitation of strain P3 in any environmental application. (nature.com)
Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
Here, both research concerning the dissection of fruit quality related metabolic changes, the transcriptional and post-translational regulation of these metabolic pathways are reviewed. (biomedcentral.com)
As the most important traits for fresh market and processing, the appearance and internal quality of fruit are formed by dramatic changes in the activities of a series of metabolic pathways during the ripening process. (biomedcentral.com)
Here, we focus on the recently obtained knowledge considering structural genes as well as transcriptional and post-translational regulators involved in the metabolic pathways underlying both appearance and internal quality such as those involved in chlorophyll, carotenoid, cell wall, central and secondary metabolism (Table 1 , Figure 1 ). (biomedcentral.com)

Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. (biomedcentral.com)

His Toluene dioxygenase research includes themes of Indole test, Toluene, Spectrophotometry and Tryptophanase. (research.com)

Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. (lbl.gov)

These immobilized laccases all showed higher efficiency than free laccase for oxidative polymerization of catechol and hydroquinone. (bvsalud.org)

Oxygenases can further be grouped into two categories i.e. monooxygenases and dioxygenases on the basis of number of oxygen atoms used for oxidation. (biomedcentral.com)

To date little is known about the microbial degradation of OPP. (nature.com)
The microbial degradation of native lignin by basidiomyce- citrant and stable in the environment. (lu.se)

Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)

The bacterial catabolism of orcinol (5-methyl-1,3-benzenediol) can be used as a model for the metabolism of other members of the resorcinol (1,3-benzenediol) compound family. (ethz.ch)
These results contribute to a better more biomass-derived carbohydrates are also used for produc- understanding of the aromatic metabolism of P. putida in tion of chemicals, a further valorization of lignin is an essential terms of growth and uptake rates, which will be helpful for component. (lu.se)

Comment: In MetaCyc pathway anthranilate degradation I ( link ), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. (lbl.gov)

Overview: Tryptophan degradation in GapMind is based on MetaCyc degradation pathways I via anthranilate ( link ), II via pyruvate ( link ), or IX via 3-hydroxyanthranilate ( link ). (lbl.gov)
In MetaCyc pathway anthranilate degradation IV ( link ), anthranilate hydroxylase/monooxygenase (hpaH) yields 3-hydroxyanthranilate. (lbl.gov)
Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd. (lbl.gov)

The other MetaCyc pathways do not yield fixed carbon or are not reported in prokaryotes, and are not included. (lbl.gov)
Pathways X and XIII yield indole-3-propionate, which may spontaneously oxidize to kynurate, but kynurate catabolism is not reported. (lbl.gov)

For example, pathway IV yields indole-3-lactate, which could potentially be oxidized to indole-3-acetate, which has a known catabolic pathway, but no prokaryotes are known to consume tryptophan this way. (lbl.gov)
Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. (nature.com)
The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)

1,2-CTD uses Fe3+ as a cofactor to cleave the carbon-carbon bond between the phenolic hydroxyl groups of catechol, thus yielding muconic acid as its product. (wikipedia.org)
In contrast, 2,3-CTD utilizes Fe2+ as a cofactor to cleave the carbon-carbon bond adjacent to the phenolic hydroxyl groups of catechol, thus yielding 2-hydroxymuconaldehye as its product. (wikipedia.org)
The second catechol hydroxyl group on carbon 3 (C3) is coordinated to Fe3+ after its deprotonation by the Tyr200 ligand. (wikipedia.org)
Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. (biomedcentral.com)

nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
Diverse organization of genes of the β-ketoadipate pathway in members of the marine Roseobacter lineage. (microbiologyresearch.org)
B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)

In the pyrene and fluoranthene degradation experiment using a minimum medium, B. fungorum isolate no. 1 and M. gilvum isolate no. 13 were able to degrade 98.6% + 1.9 of pyrene after 20 days, and 99.9% ± 0.1 after a 16-day incubation period, respectively. (omicsonline.org)

Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known ( link ), and anthraniloyl-CoA reductase (the only anaerobic route known, link ) has not been linked to sequence. (lbl.gov)

The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. (lookformedical.com)

The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)

An organism which can initiate the pathway is given, but other organisms may also carry out later steps. (ethz.ch)

Comment: MetaCyc pathway 3-oxoadipate degradation ( link ) involves activation by CoA (using succinyl-CoA) and a thiolase (succinyltransferase) reaction that splits it to acetyl-CoA and succinyl-CoA. (lbl.gov)
In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. (qmul.ac.uk)

Catechol degradation to HPD II also involves xylE and HPD, link . (lbl.gov)

There are two separate search engines for searching into the database i.e. mono and dioxygenases database respectively. (biomedcentral.com)

this has seen reduction in SO 2 emissions across Europe by more than 60% between 1990 and 2004 (Vestreng et al. (springer.com)
reproductive enthusiastic feed 's the Common reduction of 1,3-diaminobenzenes from entirely gradual risks. (xn--drpverein-rahe-vpb.de)

Anatomy7

Organisms26

Diseases2

Chemicals and Drugs142

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes51

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Compounds11

Hydrocarbons6

Pseudomonas9

Bacteria4

Enzyme3

Meta-cleavage3

Polycyclic1

Cleavage5

Characterization3

Gene6

Extradiol dioxygenase1

Naphthalene dioxygenase2

Biodegradation1

Benzoate1

Hydroxylation1

Metabolic6

Enzymes2

Toluene1

Hydrolase1

Oxidative1

Oxidation1

Microbial degradation2

Dehydrogenase2

Metabolism2

Cleaves1

Anthranilate3

Yield2

Catabolic3

Carbon4

Genes3

Pyrene1

Reductase1

Substrate1

Mechanism1

Organism1

Reaction2

Involves1

Separate1

Reduction2