Pathway chromatin assembly factor 1. Medical search. Frequent questions

ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization. (ens-lyon.fr)
Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
The main proteins found in chromatin are called histones. (hstalks.com)
The additional aspect that histones can bring in the chromatin organization is a source of information. (hstalks.com)
First, you have the DNA in the form of a helix, which is wrapped around the core histones to form the nucleosome-the sort of tennis ball that was represented before- which then folds up further to give chromatin fibers, which further fold up and then organize in the nucleus to form different domains. (hstalks.com)
While canonical histones H3.1 and H3.2 are synthetized and loaded during DNA replication, the histone variant H3.3 is expressed and deposited into the chromatin throughout the cell cycle. (aging-us.com)
Among these regulatory processes, core and linker histones are subjected to a large pattern of posttranslational modifications (PTMs) that influence chromatin state and DNA accessibility [ 4 - 7 ]. (aging-us.com)
Along with PTMs, the partial or complete disassembly of nucleosomes allows the exchange and degradation of pre-existing histone proteins, with the incorporation of newly synthesised histones onto chromatin that can eventually result in the resetting of previous epigenetic marks. (aging-us.com)
Here, we identify mutants of histones H3.1 and H3.3 that are unable to interact with human ASF1A and ASF1B isoforms but that are still competent to bind CAF-1 and HIRA, respectively. (omicsdi.org)
We show that these mutant histones are inefficiently deposited into chromatin in vivo. (omicsdi.org)
Consistent with this, biochemical analysis of wild-type and phospho-mimetic Asf1a shows that phosphorylation enhances binding to histones and the downstream chaperones CAF-1 and HIRA. (omicsdi.org)
We thus propose that TLK signalling promotes histone supply in S phase by targeting histone-free Asf1 and stimulating its ability to shuttle histones to sites of chromatin assembly. (omicsdi.org)
These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure (Santisteban, 2000). (sdbonline.org)
Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. (biomedcentral.com)
Following their synthesis, histones undergo a series of maturation steps to prepare them for deposition onto chromatin. (biomedcentral.com)
We find that depletion of JMJD1B increases the protein levels of the histone chaperone tNASP leading to an accumulation of newly synthesized histones H3 and H4 at early steps of the histone maturation cascade, which perturbs chromatin assembly. (biomedcentral.com)
At the molecular level, we find that upon reducing the JMJD1B levels, histones H3 and H4 accumulate at early stages of the processing pathway due to an accumulation of the histone chaperone tNASP. (biomedcentral.com)
We find that CAF-1 deficient cells as well as cells affected in newly-synthesized H3-H4 histones deposition during DNA replication exhibit a severe negative growth with ctf4 ∆ mutant. (cell-stress.com)
Collectively, our results suggest that the deposition by CAF-1 of newly-synthesized H3-H4 histones during DNA replication creates a chromatin environment that favors sister chromatid cohesion and maintains genome integrity. (cell-stress.com)

A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)
This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
DNA in the nucleus is present in the form of chromatin, and that consists of a complex together with proteins. (hstalks.com)
These dedicated proteins are generally known as histone chaperones and effectively control histone supply and chromatin dynamics [ 8 ]. (aging-us.com)
The genome of eukaryotic cells is organised into chromatin, a dynamic structure composed of DNA and specialised proteins that packages DNA to fit inside the nucleus, regulates gene expression and ensures genome stability. (dundee.ac.uk)
Molecular analysis suggested that Esc2p contributed to silencing in a manner similar to Sir1p and probably helped recruit or stabilize the other Sir proteins, while Htz1p present at HMR assembled a specialized chromatin structure necessary for silencing (Dhillon, 2000). (sdbonline.org)
The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
Recent multi-omics studies have revealed the heterogeneity and complexity of tumor features such as their genetic mutations, transcriptome, proteins, and signaling pathways. (rsc.org)
In particular, we will discuss the modifications in intrinsic and extrinsic apoptotic pathways caused by proteins encoded by HPV early genes. (mdpi.com)
We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)
The plant homeodomain (PHD) finger is a C4HC3 zinc-finger-like motif found in nuclear proteins thought to be involved in epigenetics and chromatin-mediated transcriptional regulation. (embl-heidelberg.de)
The PHD finger and the bromodomain are small protein domains that occur in many proteins associated with phenomena related to chromatin. (embl-heidelberg.de)

In addition to mutations in TP53 and KRAS, we identify genetic alterations in chromatin remodelling genes, ARID1A and ARID1B, in histone methyltransferase MLL3, in histone deacetylase modifier SPOP and in chromatin assembly factor BAZ1A, in nearly two thirds of cases. (nih.gov)
Alterations in genes with potential clinical utility are observed in more than three quarters of the cases and included members of the PI3-kinase and homologous DNA repair pathways. (nih.gov)
To this end, our specific objectives are to determine the nature and mechanism of action of both the general transcription initiation factors that are commonly used by all genes and the gene- and cell type-specific factors that directly regulate target genes in response to various growth, developmental and viral stimuli. (rockefeller.edu)
The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
Similarly, the transcription of small structural RNA genes (5S RNA, tRNA) by RNA polymerase III involves the assembly of common factors (TFIIIC and TFIIIB) into highly stable complexes via an ordered pathway. (rockefeller.edu)
Similarly, the single-cell assay for transposase-accessible chromatin sequencing showed increased chromatin accessibility at genes associated with myeloid differentiation, including CD11b, CD11c, and IRF8. (nih.gov)
We found that several genes also confer high levels of sensitivity to mAMSA including strains with mutations in HPR5, a helicase involved in funneling DNA lesion into recombination-repair pathways, ASF1, a chromatin assembly factor that is required for gene silencing, and CTF4, a DNA polymerase alpha binding protein important for genome stability. (aacrjournals.org)
In eukaryotic cells, chromatin remodeling factors regulate chromatin structure by altering the assembly, disassembly, and relocalization of nucleosomes, thereby improving the local accessibility of transcription-related factors in their chromatin DNA, which further initiates or suppresses transcription of related genes. (creativebiomart.net)
To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
Summary: Elucidate the differential role Pcl2 plays in the Polycomb repressive complex-1 and 2 with respect to the Hox gene cluster and Cdkn2a genes. (openwetware.org)
Furthermore, we describe genetic interactions between CAF-1 and essential genes involved in cohesin loading, cohesin stabilization, and cohesin component indicating that CAF-1 is crucial for viability when sister chromatid cohesion is affected. (cell-stress.com)

During DNA replication, nucleosomes are rapidly assembled on newly synthesized DNA to restore chromatin organization. (omicsdi.org)
These advances establish a third mechanism of chromatin reconfiguration, raising fundamental questions about the stability of nucleosomes in nonreplicative phases of the cell cycle and the cellular machinery responsible for incorporating histone variants into nucleosomes (Mizuguchi, 2004). (sdbonline.org)

Chromatin assembly factor 1 subunit B is a protein that in humans is encoded by the CHAF1B gene. (wikipedia.org)
The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. (wikipedia.org)
The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. (lookformedical.com)
We have shown that the invasion of DNA strands into a donor template region requires the action of the chromatin remodeling protein Rad54 that enables the recombination machinery to gain access to "closed" regions of DNA. (brandeis.edu)
Caspase-activated DNase (CAD) or DNA fragmentation factor subunit beta is a protein that in humans is encoded by the DFFB gene. (wikipedia.org)
DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. (wikipedia.org)
it acts as a CAD inhibitor and also as a chaperone for CAD synthesis assisting the correct assembly of the protein. (wikipedia.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
1) The physical link between RITS and CLRC has recently been found to be mediated by the zinc finger protein Stc1. (dundee.ac.uk)
Lipocalin 10 is essential for protection against inflammation-triggered vascular leakage by activating LDL receptor-related protein 2-slingshot homologue 1 signalling pathway. (uc.edu)
Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
This gene encodes a protein phosphatase 1 binding protein. (nih.gov)
The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. (nih.gov)
MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability. (nih.gov)
Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold. (nih.gov)
This protective effect of preaccumulated p53 was mediated, at least in part, by the increased expression of CDKN1A/p21, subsequent down-regulation of BRCA1, and impaired JNK activation accompanied by decreased association of replication protein A with chromatin. (rupress.org)
We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
We therefore asked what role JMJD1B plays in newly synthesized histone maturation by exploiting the knockdown of JMJD1B by treating HeLa cells with siRNA against JMJD1B (siJMJD1B) and observed the reduction of both JMJD1B protein on cytosolic extracts (Fig. 1 b) and mRNA levels (Fig. 1 c) 72 h after the treatment. (biomedcentral.com)
The function of this domain is not yet known but in analogy with the LIM domain it could be involved in protein-protein interaction and be important for the assembly or activity of multicomponent complexes involved in transcriptional activation or repression. (embl-heidelberg.de)
[ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

BACKGROUND: Anti-Silencing Factor 1 (ASF1) is a conserved H3-H4 histone chaperone involved in both Replication-Coupled and Replication-Independent (RI) nucleosomeassembly pathways. (ens-lyon.fr)
At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. (ens-lyon.fr)
ASF1 also provides H3.3-H4 dimers to HIRA and DAXX chaperones for RI nucleosome assembly. (ens-lyon.fr)
In asf1 KD embryos, HIRA localizes to the male nucleus but is only capable of limited and insufficient chromatin assembly. (ens-lyon.fr)
This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell.In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in KD embryos. (ens-lyon.fr)
In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. (omicsdi.org)
ASF1 is a highly conserved histone H3/H4 chaperone that synergizes in vitro with two other histone chaperones, chromatin assembly factor 1 (CAF-1) and histone repression A factor (HIRA), in DNA synthesis-coupled and DNA synthesis-independent nucleosome assembly. (omicsdi.org)
This pathway does not require interaction of ASF1 with HIRA. (omicsdi.org)

It remains inactive in growing cells while it is associated with its inhibitor (ICAD, DNA fragmentation factor 45 kDa subunit, DFFA or DFF45) resulting into a complex ICAD-CAD. (wikipedia.org)
We report the crystal structure of an ASF1a-HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1. (omicsdi.org)
Upregulation of Phosphatase 1 Nuclear-Targeting Subunit (PNUTS) Is an Independent Predictor of Poor Prognosis in Prostate Cancer. (nih.gov)
Phosphatase 1 Nuclear Targeting Subunit (PNUTS) Regulates Aurora Kinases and Mitotic Progression. (nih.gov)
Human transcription factor IIIC box B binding subunit. (nih.gov)

The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA. (lookformedical.com)

Epigenetics Chromatin, 11(1):19. (ens-lyon.fr)

Novel DNA repair pathways required for cell survival following exposure to topoisomerase II targeting agents. (aacrjournals.org)
In the absence of CTF4 , CAF-1 is essential for viability in cells experiencing replication problems, in cells lacking functional S-phase checkpoint or functional spindle checkpoint, and in cells lacking DNA repair pathways involving homologous recombination. (cell-stress.com)

Heterochromatin is a repressive form of chromatin found at repetitive sequences such as centromeres, telomeres and transposons. (dundee.ac.uk)

Histone chaperones have been implicated in nucleosome assembly and disassembly as well as histone modification. (omicsdi.org)

Although they take part in the assembly process, molecular chaperones are not components of the final structures. (lookformedical.com)
Chromatin dynamics is regulated by many factors, including histone tail modifiers, remodeling complexes, and histone chaperones ( Peterson and Almouzni, 2013 ). (molcells.org)
Human HIRA, ASF1a, ASF1b and CAF-1 are evolutionally conserved histone chaperones that form multiple functionally distinct chromatin-assembly complexes, with roles linked to diverse nuclear process, such as DNA replication and formation of heterochromatin in senescent cells. (omicsdi.org)

Eukaryotic cells compact their large genome into highly ordered chromatin structures within the nucleus. (aging-us.com)
Taken together, these data suggest that Velo is an additional new negative regulator of the IMD pathway, possibly acting in both the nucleus and cytoplasm. (cnrs.fr)

specific histone deposition pathways for each variant? (hstalks.com)
It is mainly manifested in the reduction of whole-genome DNA methylation level, hypermethylation of the promoter region, changes in specific histone modification, abnormal chromatin conformation, changes in non-coding RNA (ncRNA) molecules, etc . (creativebiomart.net)

This "in vivo biochemistry" approach has enabled us to demonstrate that there are in fact several independent, competing pathways of homologous recombination, each with its own genetic requirements. (brandeis.edu)
The dynamic nature of chromatin establishes the access to the genetic material and, as a consequence, influences a large number of biological processes, such as DNA replication, repair and transcription [ 1 , 2 ]. (aging-us.com)
Summary: They constructed a quantitative, high-density genetic interaction map in Saccharomyces cerevisiae to explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways. (openwetware.org)
Here we examined the genetic interaction between the heterotrimeric chromatin assembly factor-1 (CAF-1), a central component of chromatin assembly during replication, and the core replisome component Ctf4. (cell-stress.com)

Finally, our data indicate that the CAF-1-dependent pathway required for cohesion is functionally distinct from the Rtt101-Mms1-Mms22 pathway which functions in replicated chromatin assembly. (cell-stress.com)

Both homologous recombination, in which the ends of the broken DNA seek out intact templates with the same sequence, and nonhomologous end-joining pathways are found in Saccharomyces as they are in humans. (brandeis.edu)

Indeed, the RNAi effector complex RITS recruits to chromatin the methyltransferase complex CLRC, which is responsible for methylation of histone H3 at lysine 9 (H3K9me), a repressive chromatin mark. (dundee.ac.uk)
Recruitment of Bonus to chromatin leads to silencing associated with accumulation of the repressive H3K9me3 mark. (elifesciences.org)

In such a crowded molecular picture, recent exciting insights have uncovered the role of histone variants as key regulators of the chromatin structure. (aging-us.com)
Incorporation of histone variants confers variability to the chromatin and expands the repertoire of epigenetic marks in a functional alphabet that controls genome plasticity and dynamics [ 4 ]. (aging-us.com)
Recent studies have revealed that variants of histone H2A and histone H3 play important roles not only in gene expression but also in the repair of DNA breaks and the assembly of chromosome centromeres. (sdbonline.org)

The elementary unit of chromatin is a nucleosome in which approximately 147 bp DNA is wrapped 1.67 turns around a histone octamer ( Fierz and Poirier, 2019 ). (molcells.org)

We dissected the role of CAF-1 in the maintenance of genome stability in ctf4 ∆ yeast cells. (cell-stress.com)

Repression of TRIM13 by chromatin assembly factor CHAF1B is critical for AML development. (uc.edu)
We show that Bonus associates with the histone methyltransferase SetDB1 and the chromatin remodeler NuRD and depletion of either component releases Bonus-induced repression. (elifesciences.org)

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)
It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. (cancerindex.org)
Acly inhibition thus can promote myelopoiesis through deprivation of acetyl-CoA and altered histone acetylome to regulate C/EBP transcription factor family activity for myeloid differentiation. (nih.gov)
Figure 4: Identification of enriched motifs within the estrogen receptor binding sites and validation of transcription factor binding. (nature.com)
Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
Part of transcription factor TFIIIC complex. (nih.gov)
Purification and characterization of two forms of human transcription factor IIIC. (nih.gov)
The activity of transcription factor IIIC1 is impaired during differentiation of F9 cells. (nih.gov)
As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)

These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
Chromatin dynamics is essential for maintaining genomic integrity and regulating gene expression. (molcells.org)

So that will impose a problem of accessibility to the DNA for all DNA transactions, which thus necessitates dynamic properties in order for all the metabolic pathways of the DNA level to operate. (hstalks.com)
In this study, we demonstrate that ATP citrate lyase (Acly), which metabolizes citrate to generate cytosolic acetyl-CoA and is of clinical interest, can regulate chromatin accessibility to limit myeloid differentiation. (nih.gov)
Mechanistically, Acly deficiency altered chromatin accessibility and expression of multiple C/EBP family transcription factors known to regulate myeloid differentiation and cell metabolism, with increased Cebpe and decreased Cebpa and Cebpb. (nih.gov)

Chromatin organization is dynamically altered for diverse DNA metabolic transactions such as replication, transcription, and repair. (molcells.org)

Bonus has been implicated in embryonic development and organogenesis and shown to regulate several signaling pathways, however, its targets and mechanism of action remained poorly understood. (elifesciences.org)

Together, these studies begin to define the molecular determinants of assembly of functionally diverse macromolecular histone chaperone complexes. (omicsdi.org)

To understand the molecular biology of peptide hormone action, we studied response of budding yeast ( Saccharomyces cerevisiae ) to its peptide mating pheromones ( a -factor and α-factor). (berkeley.edu)

In the study of Ras-induced tumor development, the importance of chromatin remodeling changes in cancer is also evident, with the observation that chromatin conformation is more open in early-developing tumors and more accessible in late-stage tumor development. (creativebiomart.net)

Lysine acetylation is one of several histone modifications that have been proposed to form the basis for a mechanism for recording epigenetically stable marks in chromatin, known as the histone code. (embl-heidelberg.de)

Knowledge of the mechanisms by which fatty acids control specific gene expression may identify important risk factors for cancer and provide insight into the development of new therapeutic strategies for a better management of whole body lipid metabolism. (springer.com)
Summary: Characterize the role chromatin barriers with regard to gene expression of the irradiation-responsive enhancer region which is under silencing control via H3K27me3. (openwetware.org)

We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)

My findings suggest that Mkt1 could act in an RNA surveillance pathway, thus linking RNA metabolism with the establishment of heterochromatic silencing. (dundee.ac.uk)

By testing a series of Stc1 mutants for the ability to mediate silencing, either at centromeres or in an artificial RNAi-independent system, I discovered that the unstructured C-terminus of Stc1 is responsible for CLRC recruitment to chromatin, likely via electrostatic interactions. (dundee.ac.uk)

Furthermore, we found that both ASF1A and ASF1B participate in the DNA synthesis-independent deposition of H3.3 in HeLa cells, thus highlighting an unexpected role for ASF1B in this pathway. (omicsdi.org)

DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. (wikipedia.org)

Carriers of 1-3 risk genotypes have a significantly higher Wilms tumor risk than those without risk genotype (adjusted OR=1.49, 95% CI=1.07-2.07, P =0.020). (jcancer.org)
Wilms tumor, resulting from deviant cellular proliferation and differentiation, is currently one of the most concerned tumors in children [ 1 , 2 ]. (jcancer.org)
This tumor affects 1 in 10000 children and constitutes 8% of pediatric cancers [ 4 ]. (jcancer.org)

Chromatin compaction protects the genome and maintains genomic integrity. (molcells.org)
These results indicate the utility of genomic approaches using model organisms to identifying pathways for sensitivity to chemotherapeutic agents. (aacrjournals.org)

Heterochromatin-mediated silencing of these loci prevents aberrant chromosomal rearrangements and constitutes a platform for recruiting factors responsible for faithful chromosome segregation. (dundee.ac.uk)

We investigated its cellular localization and found it in both cytosolic and nuclear extracts derived from HeLa cells (Fig. 1 a). (biomedcentral.com)
The narrow diame- association with cellular and mitotic tubulin as ter and great length of single-walled carbon nano- well as the chromatin. (cdc.gov)

Chromatin assembly factor I (CAF-1) is required for the assembly of histone octamers onto newly-replicated DNA. (wikipedia.org)

However, the molecular details underlying histone assembly by Abo1 remain elusive. (molcells.org)

Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly. (omicsdi.org)
CAF-1 p60 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. (omicsdi.org)

We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Novel factors of chromatin stability. (cell-stress.com)

A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)

Caspase signals resulting from the activation of nuclease CAD indicate that the cell differentiation is due to a CAD modification in chromatin structure. (wikipedia.org)
The cell diversity is originated by cell differentiation, which has been attributed to the activation of specific transcription factors. (wikipedia.org)
J Cell Biol (2008) 182 (1): 197-213. (rupress.org)

Most of the general factors (classes II and III) have been purified and individual subunits cloned for further structural and functional studies. (rockefeller.edu)

Histone modifications related to both active and inhibitory chromatin can be observed during cancer progression, and these modifications can occur simultaneously. (creativebiomart.net)

These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery (Mizuguchi, 2004). (sdbonline.org)

SUMOylation influences Bonus's subnuclear localization, its association with chromatin and interaction with SetDB1. (elifesciences.org)

These various classes of cofactors, as well as others (p300/CBP, STAGA) implicated in chromatin template modifications, offer important new mechanisms for gene regulation. (rockefeller.edu)
Our studies are also directed toward the mechanisms by which these factors in turn are regulated in the various biological responses. (rockefeller.edu)
For these reasons, this pathway and the mechanisms by which TORC2 and Ypk1 are regulated are under continuing intensive study. (berkeley.edu)

Anatomy8

Organisms6

Diseases1

Chemicals and Drugs37

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes28

Information Science3

Histones19

Proteins13

Genes12

Nucleosomes2

Protein24

Asf18

Subunit5

Transcriptional1

Epigenetics1

Repair pathways2

Form of chromatin1

Disassembly1

Chaperones3

Nucleus2

Specific histone2

Genetic4

Functionally distinct1

Homologous recombination1

Repressive2

Variants3

20191

Genome stability1

Repression2

Transcription factor9

Dynamics2

Accessibility3

Organization1

Regulate1

Complexes1

Budding yeast1

Conformation1

Lysine1

Gene expression2

Regulates1

Metabolism1

Recruitment1

Deposition1

Apoptosis1

Tumor3

Genomic2

Chromosome1

Cellular2

Newly1

Molecular1

HIRA2

Stability2

Histone octamer1

Cell3

Structural1

Inhibitory1

REMODELING1

Localization1

Mechanisms3