Patch clamp electrophysiology of neurons. Medical search. Frequent questions

We use up to 12 multi-patch clamp setups to study the individual neuronal properties and quantify the principles of local connectivity between these neurons (microcircuits). (epfl.ch)
Using a combined experimental and modelling approach, neuronal parameters were extracted to measure, for the first time in the neocortex, specific changes in neuronal electrophysiology. (nih.gov)
Macklis's laboratory had for several years developed approaches to successfully transplanting developing neurons into circuitry of the cerebral cortex of mice with neurodegeneration or neuronal injury. (sciencedaily.com)
With whole-cell recording from single neurons, we found that ACh inhibited excitatory postsynaptic currents (EPSCs) while elevating neuronal firing in a dose-dependent manner. (springer.com)
Patch-clamp electrophysiology found that Rai1 deletion in these neurons decreased neuronal firing. (huanglabmcgill.org)
Primary neuronal culture is the possibility to visually identify neurons, characterize in details both action potentials and underlying ionic currents and to correlate electrophysiology and molecular biology on the same batch of neurons. (sav.sk)
In vivo electrophysiology offers the possibility to measure neuronal activity within its normal environment including all interactions with other brain parts. (sav.sk)
In this study, we examined the activity of "neuron"-like cells (expressing pan-neuronal markers or with neuronal morphology) in the gut of E11.5 and E12.5 mice using whole-cell patch-clamp electrophysiology and compared them to the activity of neonatal and adult enteric neurons. (edu.au)
SynFire iNs are generated using a patented procedure for direct reprogramming and exhibit main characteristics of human primary neurons such as expression of typical pan-neuronal markers and complex electrophysiology (e.g., spontaneous/evoked action potentials, synchronized network activity). (neucytelabs.com)
Neuronal subtype identity of excitatory iN cells has been confirmed by staining and patch clamping. (neucytelabs.com)

The LNMC has always been in the forefront in electrophysiological characterization and classification of cortical neurons. (epfl.ch)
Using 3D reconstructions of neurons our goal is to map: 1) the complete set of cortical neuron morphologies, 2) the relative composition of cortical neuron subtypes, and 3) the long range projections between cortical microcircuits and other brain structures. (epfl.ch)
Dendritic protein co-localization, AMPA receptor surface expression, synapse density, and glutamatergic synapse activity in primary cortical cultures from knock-in and wild-type littermates were assayed using immunocytochemistry and whole-cell patch clamp electrophysiology. (ozgene.com)
In cultured cortical neurons from knock-in mice, pRab10 is also increased, and reversed by LRRK2 inhibition. (ozgene.com)
We discovered that Rai1 loss from the Emx1-lineage cortical excitatory neurons enhances intrinsic excitability and glutamatergic synaptic transmission of the hippocampal dentate gyrus granule cells. (huanglabmcgill.org)
In addition, we find that each class of projection neuron exhibits a unique compliment of hyperpolarizing and depolarizing afterpotentials that further suggests that cortical neurons with different subcortical targets are distinct from one another. (nih.gov)
These cultures will contain mature, identifiable DA neurons in co-culture with excitatory cortical neurons. (uni-luebeck.de)
The fellow will lead an innovative project studying the physiology of human cortical neurons resected during neurosurgery. (uhntrainees.ca)
In this dataset, transcriptomic profiles of individual human cortical neurons were assayed by the SMART-seq v4 single nucleus RNA-seq method following acute brain slice patch-clamp electrophysiology recordings and nucleus extraction, i.e. the Patch-seq method. (nemoarchive.org)

This clamp is critical for testing voltage dependent opening dynamics of ion channels which determine a membrane's resting potentials and action potentials. (wikipedia.org)
Myelination of central neurons increases conduction velocity of action potentials and provides metabolic support to axons 1 , 2 . (biorxiv.org)
These iPSC-derived neurons were analyzed for their ability to fire action potentials by patch clamp electrophysiology. (uni-luebeck.de)
Similarly, sex differences in excitability of RSADP neurons in the gRSC are present during early adolescence, in which males fire more action potentials compared to females. (uwm.edu)

Oligomeric and monomeric αSyn were injected directly into the soma of pyramidal neurons in mouse neocortical brain slices during whole-cell patch clamp recording. (nih.gov)
Layer V pyramidal neurons are anatomically and physiologically heterogeneous and project to multiple intracortical and subcortical targets. (nih.gov)
However, because most physiological studies of layer V pyramidal neurons have been carried out on unidentified cells, we know little about how anatomical and physiological properties relate to subcortical projection site. (nih.gov)

We mapped and quantified the intracortical inputs to L2/3 neurons using laser-scanning photostimulation combined with patch clamp recordings. (biorxiv.org)
Recordings in flying flies revealed that the gain and frequency tuning of wide-field neurons change during flight, and that these effects are mimicked by the neuromodulator octopamine. (janelia.org)
17 Consistent with this model, in vivo extracellular single-unit recordings have demonstrated that only a minority of LA neurons undergo significant changes in tone-evoked firing during auditory fear conditioning. (nature.com)
Here we combine neuroanatomical tract tracing with whole cell recordings in mouse somatosensory cortex to test whether neurons with the same projection target form discrete subpopulations and whether they have stereotyped physiological properties. (nih.gov)
Each experimental treatment contained data from 3-5 animals and typically recordings from ～30 neurons. (bioxorio.com)
The whole cell patch-clamp recordings from the SCN were taken with Aliskiren (CGP 60536) recording electrodes. (bioxorio.com)
They performed whole-cell patch-clamp recordings to measure light-evoked synaptic responses in dPAG neurons as a response to channelrhodopsin-2 (ChR2) activation in the mPFC. (uk.com)
In the first part of the talk, we use data assimilation and patch-clamp recordings from the diurnal rodent Rhabdomys pumilio to build the first mathematical models of the electrophysiology of circadian neurons in a day-active species. (duke.edu)

Highly functional human iPSC-derived neurons are of particular value due to the unavailability of natural sources. (neucytelabs.com)

Recent computational modeling has proposed that the encoding of fear memories in the LA is constrained to a limited subset of neurons by the local microcircuitry through a combination of intrinsic excitability and synaptic plasticity. (nature.com)
We will investigate effects of acute and chronic in vitro and in vivo (prenatal and postnatal) administration of μ and δ opioid receptor ligands on the morphology and excitability of hippocampal neurons and on hippocampal plasticity in control and stressed rats. (sav.sk)
He worked on the electrophysiology and synaptic plasticity of the retina for more than 20 years before becoming interested in the pathophysiology of cancer! (sophion.co.jp)
After my PhD I moved to modelling, working on Spike-Timing Dependent Plasticity in single spiking neuron models, in the lab of Tomoki Fukai. (oist.jp)
I then moved to the lab of Jochen Triesch, to study plasticity in networks of spiking neurons. (oist.jp)
In particular, I am interested in the interplay between Hebbian-like types of plasticity and homeostasis, and how networks of neurons can process information while maintaining their optimal states of operating, in terms of synaptic strengths and firing rates for example. (oist.jp)
Here we provide the first demonstration of the molecular and functional characteristics of the LC-AVP system, by characterizing its receptor-specific modulation of identified LC neurons and plasticity in response to stress. (port.ac.uk)
For my first post-doc I went abroad to Rome, Italy to learn patch-clamp electrophysiology in Prof. Calabresi's laboratory as a project leader of EU collaboration (F7, REPLACES) studying synaptic plasticity after cell transplantation to the brain. (lu.se)

Using patch-clamp electrophysiology in brain slices prepared from male rats, we reveal that opioid withdrawal abruptly reduces the ability of these peptides to inhibit neurotransmission, a direct consequence of a protein kinase A (PKA)-driven increase in the synaptic activity of peptidases. (jneurosci.org)
EXPERIMENTAL APPROACH: MOR function was measured in locus coeruleus neurons, by using whole-cell patch-clamp electrophysiology, in rat and mouse brain slices (both wild-type and protein kinase C (PKC)alpha knockout mice). (bath.ac.uk)

Close-up of an electrophysiology setup where microelectrodes are used to record from neurons. (precisionary.com)
To address this, her laboratory uses various techniques to record from neurons in vivo, including two-photon calcium imaging, somatic and dendritic patch-clamp electrophysiology and optogenetics. (edu.au)

Axonal arborisation and neurite outgrowth of identified DA neurons will be assessed by confocal microscopy, and intrinsic excitability, channel function, and action potential firing will be assessed by whole cell patch-clamp technique. (uni-luebeck.de)
These mice were used to characterize the morphology and physiology of advillin-expressing enteric neurons using confocal microscopy, calcium imaging, and whole-cell patch-clamp electrophysiology. (elsevierpure.com)
High resolution confocal microscopy revealed that immunoreactivity for the AVP receptor 1b (V1b) was located on plasma membranes of noradrenergic and non-noradrenergic LC neurons. (port.ac.uk)

In the current study, we analysed the biophysical effects of Org 34167 on HCN channels in stably transfected human embryonic kidney 293 (HEK293) cells and mouse layer V neurons using patch-clamp electrophysiology, and we utilised three high-throughput screens for depressive-like behaviour to assess the activity of Org 34167 in mice. (frontiersin.org)
To monitor brain activity in mice and other animals, neuroscientists often rely on a well-established technique called patch clamping, which can ignite career-questioning frustration, as electrical noise spoils seemingly good data and cells begin to die after only a few seconds of recording. (the-scientist.com)
Neuron transplants have repaired brain circuitry and substantially normalized function in mice with a brain disorder, an advance indicating that key areas of the mammalian brain are more reparable than was widely believed. (sciencedaily.com)
Collaborators from Harvard University, Massachusetts General Hospital, Beth Israel Deaconess Medical Center (BIDMC) and Harvard Medical School (HMS) transplanted normally functioning embryonic neurons at a carefully selected stage of their development into the hypothalamus of mice unable to respond to leptin, a hormone that regulates metabolism and controls body weight. (sciencedaily.com)
These mutant mice usually become morbidly obese, but the neuron transplants repaired defective brain circuits, enabling them to respond to leptin and thus experience substantially less weight gain. (sciencedaily.com)
Postdocs Artur Czupryn and Maggie Chen, from Macklis's and Flier's labs, respectively, transplanted and studied the cellular development and integration of progenitor cells and very immature neurons from normal embryos into the hypothalamus of the mutant mice using multiple types of cellular and molecular analysis. (sciencedaily.com)
Using cell type-specific Cre-LoxP and CRISPR -mediated Rai1 deletions, we uncovered that Rai1 functions in a subset of Bdnf-producing neurons by maintaining energy homeostasis in mice. (huanglabmcgill.org)
Using a CRISPR activation system , we developed the first gene therapy (more specifically, cis-regulatory therapy) that enhances the expression of the remaining healthy Rai1 allele in the hypothalamic PVH neurons of SMS mice. (huanglabmcgill.org)
Treatment also significantly preserved motor neurons and attenuated astrocyte and microglial activation in mice. (edu.au)
Further, the PKC component of morphine-induced desensitization was absent in locus coeruleus neurons from PKCalpha knockout mice. (bath.ac.uk)
APPROACH: Pulsed light or biphasic electrical pulses were delivered to cochlear spiral ganglion neurons of acutely deafened mice, either as individual stimuli or as hybrid stimuli for which the timing of the electrical pulse had a varied delay relative to the start of the optical pulse. (edu.au)

Advillin is used as a marker for primary afferent neurons in dorsal root ganglia. (elsevierpure.com)
Using retrograde tracing, trans-synaptic rabies labelling, and ex vivo electrophysiology the research group showed that layer 5 glutamatergic mPFC neurons made monosynaptic links onto glutamatergic neurons in the dorsal PAG (dPAG). (uk.com)
Sensory neurons, termed nociceptors, are derived from dorsal root ganglia (DRG) and can undergo changes in membrane excitability during chronic pain. (tdl.org)

Initially the need for direct contact between the pipette and the cell membrane limited patch clamp's use to cultured cell preparations. (wikipedia.org)
The chance of the pipette tip clamping off target was reduced via application of positive pressure during insertion or use of a cleaning pipette to ensure the main seal was not damaged. (wikipedia.org)
A highly invasive method that involves attaching a microscopic glass pipette to a single neuron to measure its electrical signals. (precisionary.com)
Neurobiotin pipette filling during patching and tyrosine hydroxylase immunofluorescence staining post-fixation confirmed the identity of the examined neurons. (uni-luebeck.de)

However, the defining cellular properties of neurons within a memory trace remain incompletely understood. (nature.com)
Patch-clamp electrophysiology is a powerful technique used by neurophysiologists to investigate the electrical properties of neurons. (precisionary.com)

Patch-seq is a specialized form of the patch clamp recording technique, the gold standard of single cell electrophysiological studies for its millisecond resolution of cellular electrophysiology, its ability to detect currents of specific ions, and perhaps most importantly for its ability to form a voltage clamp on the cell membrane. (wikipedia.org)
While other modern recording techniques such as multi-electrode array and fluorescent calcium sensors have emerged as alternatives which can record from a great deal more neurons at once, patch clamp offers millisecond temporal resolution and the ability to study specific ionic currents. (wikipedia.org)
Fibrotic scar model and TGF-b1 differently modulate action potential firing and voltage-dependent ion currents in hippocampal neurons in primary culture. (sav.sk)

Finally, oxA/hcrt-1-mediated excitatory synaptic transmission onto VTA neurons was not potentiated following an arousing, aversive stimulus, suggesting that oxA/hcrt-1-mediated glutamatergic synaptic transmission was potentiated selectively with highly salient positive reinforcers. (jneurosci.org)

CuII(atsm) therefore represents a potential new class of neuroprotective agents targeting multiple major disease pathways of motor neurons with therapeutic potential for ALS. (edu.au)
As a trained electrophysiologist, he can use patch clamp electrophysiology - for instance, attaching electrodes directly to motor neurons, the cells that use electrical signals to stimulate movement in the fish - as a means to examine the functional connectivity between motor neurons and muscle cells. (braincanada.ca)
This will be invaluable in unlocking the roles of these two genetic mutations at the critical neuromuscular junction where motor neurons connect to muscle. (braincanada.ca)

It also reduced I h -mediated sag in mouse neurons. (frontiersin.org)

Around 30-40% of neuron-like cells at E11.5 and E12.5 fired APs, some of which were very similar to those of adult enteric neurons. (edu.au)

Previously, we have demonstrated that small networks of cultured hippocampal neurons exhibit persistent activity in response to brief stimuli applied to single cells [ 26 ], reminiscent of reverberatory activity in the hypothetical cell assembly proposed by Donald Hebb as a network basis of "online" working memory [ 27 ]. (springer.com)
We will analyze the effect of acute (seconds to minutes) and chronic (hours to days) in in vitro and in vivo (prenatal and postnatal) administration of DOR ligands on the morphological and electrophysiological properties of rat hippocampal neurons and compare them with effects of MOR ligands and with effects of ligands specific for MOR-DOR heteromers. (sav.sk)
Excitability will be investigated in primary culture of hippocampal neurons by patch clamp and in situ by in vivo electrophysiology. (sav.sk)

Purinergic regulation of brain catecholamine neurotransmission: In vivo electrophysiology and microdialysis study in rats. (sav.sk)

Additionally, dendritic complexity and branching is reduced in male RSADP neurons compared to male RS neurons, whereas dendritic complexity is similar in female RS and RSADP neurons. (uwm.edu)

We intend to further validate our findings and examine the DA metabolism and electrophysiological characteristics in VPS35 mutant neurons. (uni-luebeck.de)
Effect of physical exercise and acute escitalopram on the excitability of brain monoamine neurons: in vivo electrophysiological study in rats. (sav.sk)
Here I independently set up a new electrophysiological platform to analyse functional integration of in vivo reprogrammed cells and stem-cell derived neurons. (lu.se)

Through this work, Neural Networks Group will not only illustrate how sensory information is received, transformed and modulated in neurons but also how this processing of synaptic input contributes to the overall neural network activity underlying learning and behaviour. (edu.au)
Nociceptors, sensory neurons in the DRG that detect damaging or potentially damaging stimuli, are key drivers of neuropathic pain. (tdl.org)
ATP-gated P2X3 receptors are important transducers of nociceptive stimuli and are almost exclusively expressed by sensory ganglion neurons. (biomedcentral.com)
Even though the P2X3 receptor desensitizes rapidly (and, thus, self-limits its function), it can elicit fast, strong sensory neuron depolarization and firing which are actually enhanced in pathological pain states [ 4 - 8 ]. (biomedcentral.com)

Genetically silencing wide-field neurons increased behavioral responses to slow-motion stimuli. (janelia.org)
In males, fear learning or exposure to novel stimuli reduces excitability of RSADP neurons in the early adolescent, mid-adolescent, and adult gRSC. (uwm.edu)
However, in females, trace fear memory formation or exposure to novel stimuli suppresses excitability of both RS and RSADP neurons only in the adult gRSC. (uwm.edu)

We found that Arc- expressing neurons have enhanced intrinsic excitability and are preferentially recruited into newly encoded memory traces. (nature.com)

This NIH grant U01DA052713 (PI: Shen, Yin) studies cis-regulatory chromatin interactions, open chromatin peaks, and transcriptomes for radial glia, intermediate progenitor cells, excitatory neurons, and interneurons isolated from mid-gestational human cortex samples. (nemoarchive.org)

The morphology as well as the intrinsic membrane and firing properties of corticothalamic and corticotrigeminal neurons differ from those of callosal and corticostriatal neurons. (nih.gov)

The aim of the Channelome project is to characterize biophysics of these ion channels in a controlled and consistent environment with an automated patch clamp setup. (epfl.ch)

IPSCs are further differentiated into mature, postmitotic neuron-like phenotype. (semmelweis.hu)
However, it remains unclear whether this function is relevant for postmitotic neurons and underlies the cerebellar atrophy, since ATM is cytoplasmic in postmitotic neurons. (preprints.org)

More than 350 ion channels are responsible for the electrical diversity of neocortical neurons. (epfl.ch)

Learning opportunity to acquire skills in the following techniques: quantitative RT-PCR, immunocytochemistry, patch clamp electrophysiology. (semmelweis.hu)

We demonstrated that in mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. (biomedcentral.com)

Using patch clamp electrophysiology, we define a novel mechanism by which core clocks regulate membrane excitability. (ox.ac.uk)
These techniques allow us to measure the electrical activity of neurons, giving us information about their excitability, firing patterns, and synaptic connections. (precisionary.com)
We combined iDISCO+ whole brain imaging and activity mapping, in vivo EEG, ex vivo field recording, and patch-clamp electrophysiology experiments to understand how Rai1 loss in different population of neurons impact brain excitability. (huanglabmcgill.org)

Because amygdala neurons mediate relapse, and are highly opioid sensitive, we hypothesized that opioid withdrawal would induce adaptations in these neurons, opening a window of disrupted emotional learning circuit function. (jneurosci.org)
Using a fluorescence-based Arc reporter, we were able to visually identify the distinct subset of lateral amygdala (LA) neurons activated during auditory fear conditioning. (nature.com)

Whole cell patch-clamp electrophysiology. (bioxorio.com)
The access resistance of these cells ranged from 15 to 40 MΩ in the whole cell voltage-clamp configuration while the cell capacitance was typically between 6 and 18 pF. (bioxorio.com)
Using whole-cell patch-clamp electrophysiology and morphological analysis, we determined that N-methyl-D-aspartate (NMDA) receptor blockers reduce the ADP in female but not male RSADP neurons. (uwm.edu)
Whole-cell patch clamp electrophysiology revealed that the pharmacological activation of V1b receptors significantly increased the spontaneous activity of 45% (9/20) of recorded noradrenergic neurons, with the remaining 55% (11/20) of cells exhibiting a significant decrease in their basal firing patterns. (port.ac.uk)

neuropeptide vasoactive intestinal peptide (VIP) is expressed at high levels in the neurons of the suprachiasmatic nucleus (SCN). (bioxorio.com)
Cranial visceral afferents, which innervate the heart and lungs, synapse centrally onto neurons within the medial portion of the nucleus tractus solitarius (NTS). (silverchair.com)

We then examined the pan-neural response of medulla neurons and found prominent selectivity for light-on and light-off in layers of the medulla associated with two anatomically derived pathways (L1/L2 associated) [14, 15]. (janelia.org)
While these and follow-up experiments appeared to rebuild brain circuitry anatomically, the new neurons' level of function remained uncertain. (sciencedaily.com)

A method that involves recording the electrical signals from multiple neurons simultaneously using electrodes placed directly onto brain tissue. (precisionary.com)

In second-order NTS neurons, isoflurane enhances phasic inhibitory transmission via postsynaptic gamma-aminobutyric acid type A receptors while suppressing excitatory transmission through presynaptic mechanisms. (silverchair.com)

2.) DA neurons were differentiated from isogenic VPS35 wild-type and p.D620N mutant iPSC lines. (uni-luebeck.de)
Further, treatment with the dopamine transporter inhibitor GBR 12909 resulted in increased DA levels in wild-type but not p.D620N neurons. (uni-luebeck.de)

It is difficult to do both serious theory (and by this, I mean not only simulating models but also analyzing them and making predictions) and patch clamp experiments to test it. (romainbrette.fr)

Genetic engineering techniques allow researchers to tag neurons of interest with fluorescent markers that glow upon neural activation, but capturing those brain areas in action, both as distinct neural circuits and at the resolution of single cells, can be hard. (the-scientist.com)

Degeneration of DA neurons plays an important role in Parkinson disease and there is, therefore, a great need for studies in the affected cell type. (uni-luebeck.de)
Amyotrophic lateral sclerosis (ALS) is a progressive paralyzing disease characterized by tissue oxidative damage and motor neuron degeneration. (edu.au)

This ability to form the voltage clamp on a membrane patch gives the technique its name. (wikipedia.org)

We are seeking a highly motivated Postdoctoral Fellow with a background in experimental neuroscience and patch-clamp electrophysiology and an interest in data science and genomics. (uhntrainees.ca)

Scientists have identified neurons in the medial prefrontal cortex (mPFC) that project to the brainstem to prevent social animals from acting on impulsive instincts in certain contexts. (uk.com)

These results provide direct evidence that motion is computed in parallel light-on and light-off pathways, demonstrate that this selectivity emerges in neurons immediately downstream of the LMCs, and specify where crucial elements of motion computation occur. (janelia.org)

The present study demonstrates the successful generation of a new tool to identify living dopaminergic (DA) neurons, the affected cell type in Parkinson disease, and the examination of DA release and stress susceptibility in VPS35 mutant DA neurons. (uni-luebeck.de)
In-line with this hypothesis is the observation of increased susceptibility to MPP+ mitochondrial toxin found in VPS35 mutant neurons. (uni-luebeck.de)

As neural tissues are one of the most transcriptomically diverse populations of cells, classifying neurons into cell types in order to understand the circuits they form is a major challenge for neuroscientists. (wikipedia.org)
These techniques allow us to measure the electrical signals produced by neurons, giving us information about the functioning of different brain regions and the timing and coordination of neural activity ( Figure 1 ). (precisionary.com)

2 , 3 In particular, long-term N -methyl- D -aspartate (NMDA) receptor-dependent synaptic potentiation of glutamatergic inputs onto LA principal neurons remains the leading candidate mechanism for fear memory encoding. (nature.com)
Specific isoforms of PKC were inhibited by using inhibitors of the receptors for activated C-kinase (RACK), and in vivo viral-mediated gene-transfer was used to transfect neurons with dominant negative mutants (DNMs) of specific G-protein-coupled receptor kinases (GRKs). (bath.ac.uk)
Thus, we investigated changes in P2X3 receptor distribution in the lipid raft membrane compartment, their phosphorylation state, as well as their function with patch clamping. (biomedcentral.com)
In contrast, immunoreactivity for the V1a receptor was exclusively located on LC noradrenergic neurons. (port.ac.uk)

We next examined the activity of prominent interneurons within each pathway (Mi1 and Tm1) and found that these neurons have corresponding selectivity for light-on or light-off. (janelia.org)

ii) Assess these neurons for morphological and physiological phenotypes. (uni-luebeck.de)

Intriguingly, only a limited subset of neurons appears to be recruited during fear memory encoding. (nature.com)

Immature active responses, mediated in part by TTX- and lidocaine-insensitive channels, were observed in most cells at E11.5 and E12.5, but not in P0/P1 or adult neurons. (edu.au)
We find that the electrical activity of circadian neurons is similar overall between nocturnal and diurnal rodents but that there are some interesting differences in their responses to inhibition. (duke.edu)

We investigated the mechanisms underlying MOR desensitization, in adult mammalian neurons, caused by morphine (a partial agonist in this system) and DAMGO (a high-efficacy agonist). (bath.ac.uk)

In 2005, Jeffrey Flier, then the George C. Reisman professor of medicine at BIDMC, published a landmark study, also in Science , showing that an experimental drug spurred the addition of new neurons in the hypothalamus and offered a potential treatment for obesity. (sciencedaily.com)

For example, cuprizone-induced demyelination promotes hyper- and depolarizing shifts of the resting membrane potential of auditory thalamocortical pathway neurons and reduction in action potential firing of primary auditory cortex (A1) neurons 7 . (biorxiv.org)

CONCLUSIONS AND IMPLICATIONS: In mature mammalian neurons, different MOR agonists can induce MOR desensitization by different mechanisms, morphine by a PKCalpha-mediated, heterologous mechanism and DAMGO by a GRK-mediated, homologous mechanism. (bath.ac.uk)

Injury to these neurons causes activation of translation regulation signaling, including the mechanistic target of rapamycin complex 1 (mTORC1) and mitogen-activated protein kinase interacting kinase(MNK) eukaryotic initiation factor (eIF) 4E pathways. (tdl.org)

These systems generally use a low-power microscope objective for tracking the whole animal, coupled with a separate high-power objective for zooming in on a particular location in a worm's brain to observe neurons that have been genetically manipulated to fluoresce when active. (the-scientist.com)

Anatomy22

Organisms5

Diseases1

Chemicals and Drugs18

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes13

Disciplines and Occupations3

Neuronal10

Cortical9

Action potentials4

Pyramidal neurons3

Recordings8

IPSC-derived neurons1

Plasticity8

Slices2

Record from neurons2

Confocal microscopy3

Mice11

Dorsal3

Pipette4

Properties of neurons2

Currents3

Synaptic transmission1

Motor neurons3

Mouse neurons1

Enteric neurons1

Hippocampal neurons3

Vivo electrophysiology1

Dendritic1

Electrophysiological3

Sensory4

Stimuli3

Intrinsic excitability1

Progenitor cells1

Morphology1

Characterize1

Postmitotic2

Neocortical1

Immunocytochemistry1

Endogenous1

Excitability3

Amygdala2

Whole-cell4

Nucleus2

Anatomically2

Electrodes1

Enhances1

D620N2

Experiments1

Markers1

Degeneration2

Voltage clamp1

Neuroscience1

Medial1

Downstream1

VPS352

Neural2

Receptor4

Interneurons1

Morphological1

Subset1

Responses2

Adult1

Experimental1

Resting membrane1

Induce1

Pathways1

Genetically1