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CentrifugationParticle SizeCentrifugation, Density GradientCentrifugation, ZonalCentrifugation, IsopycnicMicroscopy, ElectronCell FractionationVirionMetrizamideMolecular WeightElectrophoresis, Polyacrylamide GelUltracentrifugationSignal Recognition ParticleCell LineSubcellular FractionsCell MembraneSilicon DioxidePovidoneVirus AssemblyFiltrationNanoparticlesColloidsKineticsSubmitochondrial ParticlesAerosolsFreeze FracturingVaccines, Virus-Like ParticleCell SeparationMolecular Sequence DataChromatography, GelTritiumHypergravityCapsidLatexCattleMicrospheresSolubilityInclusion Bodies, ViralViral ProteinsLiverRibonucleoproteinsHydrogen-Ion ConcentrationParticulate MatterSucroseTemperatureOrganoidsRNA, ViralRabbitsCells, CulturedVirus ReplicationCapsid ProteinsDefective VirusesMethodsAlpha ParticlesPhosphotungstic AcidDNA, ViralAmino Acid SequenceCytoplasmCesiumMicroscopy, Electron, ScanningMacromolecular SubstancesLipoproteins, HDLNucleoproteinsEscherichia coliTime FactorsDetergentsMicroscopy, Electron, TransmissionPolyethylene GlycolsProtein BindingBase SequenceSurface PropertiesPhospholipidsAir PollutantsPolystyrenesLysosomesChromatographyRibosomesInhalation ExposureVehicle EmissionsDNAPowdersAcid PhosphataseLipoproteinsVirus CultivationCell NucleusElectrophoresisRNAChemical PrecipitationVirosomesAdsorptionErythrocytesCytoplasmic GranulesMembranesCricetinaePhagocytosisMembrane ProteinsApolipoprotein A-IProteinsFreeze EtchingMicrosomes
Differential centrifugationPolystyrene ParticlesDensityNanoparticlesFiltration6000DownstreamSucroseWastewaterDensitiesElectron microscopyMicroparticlesSolidsSphericalAssaysSizeEmulsionEthylene GlycolFluidInksPrecipitationViralMagneticPoorlyRetentionAbsenceEfficiencyLargePresentBindSeparationSmallCarbonMethodTemperatureDetectionTimeEffectsStudySeparateVirusStrategiesTypesProductDistribution
Differential centrifugation3
Polystyrene Particles2
  • The polystyrene particles are prepared by using emulsion polymerization process. (spherotech.com)
  • The BD™ CompBead Plus Anti-Mouse Ig, κ beads are polystyrene particles used to establish fluorescence compensation settings for multicolor flow cytometric analyses. (bdbiosciences.com)
Density10
  • The particle immersed in the flow (assumed Stokesian) has a Stokes number St=1 and a particle-to-fluid density ratio ϱ which is assumed as the second parameter of this study. (ensam.eu)
  • CsCl density gradient centrifugation indicated that almost all of the NP protein in the cells had assembled into nucleocapsid-like structures. (nih.gov)
  • At certain centrifugal forces, there is a high probability of extracting/pelleting other particles with the same buoyant density, size, and mass as the target exosomes. (pall.com)
  • Researchers have attempted to overcome the low-purity challenges of the differential ultracentrifugation method through density-gradient centrifugation. (pall.com)
  • This problem can be overcome with the use of moving-zone density-gradient centrifugation, a method that separates particles by size and density. (pall.com)
  • In this way, particles with the same density can be separated based on size. (pall.com)
  • In October and December, fresh ejaculate was fractionated by centrifugation through a discontinuous density gradient of polyvinylpyrrolidone-coated silica particles (Percoll((R))**) to separate motile spermatozoa from other cells and seminal plasma. (cdc.gov)
  • The physician who performed the inseminations reported that in January 1990 a second HIV-1-discordant couple (i.e., seropositive husband with hemophilia, sero negative wife) underwent one insemination using the same density gradient centrifugation procedure. (cdc.gov)
  • Leukocytes remained in all four samples after simple centrifugation and washing and in two of three samples tested after density gradient centrifugation. (cdc.gov)
  • Particles/cells that have a density higher than the carrier medium will migrate to the acoustic pressure node in a microchannel under the influence of the acoustic standing wave force. (lu.se)
Nanoparticles3
  • toxic metal and silicate particles including nanoparticles are present in both the cigarette fluid and aerosol. (wakeup-world.com)
  • His research is based on a previous ERC grant on graphene nanoparticles, in which centrifugation was used to sort particles by size classes. (tudelft.nl)
  • Our goal in the present study nanoparticles (NPs) is associated with adverse opposite initial events of AM-particle interac- was to determine the long-term alveolar reten- cardiovascular effects in susceptible parts of tion lead to the same pulmonary clearance tion of these NPs and to compare our results populations (Ibald-Mulli et al. (cdc.gov)
Filtration5
60004
  • MET ONE 6000 air particle counters are a compact design with flexible sizing, flow rate and communication options that address the specific needs of cleanroom environments within the pharmaceutical, semiconductor, hard disk drive and flat panel display industries providing ISO 21501 accuracy for continuous particle monitoring. (beckman.com)
  • Reduce initial installation cost with MET ONE 6000 remote air particle counter's integrated multi-parameter measurements. (beckman.com)
  • The centrifuge speed was varied from 6000 to 12000 rpm and the centrifugation time was varied from 30 to 105 min. (scialert.net)
  • The four bottles placed inside a high speed centrifuge (Sorvall Evolution RC) with rotor used is SLA-1000 and centrifuged for different speeds ranging from 6000 to 12000 rpm (the angular velocities ω of the rotor ranging from 628.32 to 1256.64 rad sec -1 ) and different times ranging from 30 to 105 min, all experiments were run at 30°C. Same coconut milk used on each speed and centrifugation time. (scialert.net)
Downstream2
  • After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be evaluated in downstream applications such as flow cytometry. (bdbiosciences.com)
  • Homogenization of the hop particles continues during transfer to downstream centrifugation, thereby improving the separation efficiency and product clarity. (mbaa.com)
Sucrose1
Wastewater2
  • Amalgam separators remove amalgam particles from the wastewater to reduce the amount of amalgam entering the sewage system. (ada.org)
  • Homogenizing samples can also include procedures to break up wastewater solids and disaggregate virus particles, such as by sonication. (cdc.gov)
Densities1
  • Sometimes, gravity separation may be too slow because of closeness of the densities of the particles and the fluid, or because of association forces holding the components together. (scialert.net)
Electron microscopy1
  • The particles are characterized by electron microscopy, dynamic light scattering, Z potential, and Raman spectroscopy, confirming the replacement of the ligands on the metal particle surface. (bsz-bw.de)
Microparticles1
Solids2
  • Generally peeler centrifuges are used to separate solids, usually fine particles from suspension liquid feed mixtures. (wikipedia.org)
  • For primary sludge samples, centrifugation is the most effective way to concentrate solids. (cdc.gov)
Spherical1
  • A small spherical rigid particle in a cylindrical cavity is considered. (ensam.eu)
Assays1
  • Cartomizer fluid with tin particles was cytotoxic in assays using human pulmonary fibroblasts. (wakeup-world.com)
Size8
  • Nanocarriers, with the properties of smaller particle size, and long circulation time, would be advantageous in diagnostic and therapeutic applications. (hindawi.com)
  • For example, when nickel particles are reduced in size to the nanometer range (one billionth of a meter wide) they may actually become more toxic to the endocrine system as now they are capable of direct molecular interaction with estrogen receptors in the body, disrupting their normal structure and function. (wakeup-world.com)
  • What is the particle size limit for microplastics visual sorting? (researchgate.net)
  • Nevertheless, visual sorting becomes more difficult as particle size get smaller, and is time-consuming and is more likely to fall into misidentification errors. (researchgate.net)
  • The size of particles is determined by either NICOMP Laser Particle Sizer or visually by Scanning Electron Microscope. (spherotech.com)
  • The unoptimised preparations of soluble and insoluble CDPs displayed very small particle size distributions, while the prepared polymers currently have different complexation properties to those of their classically prepared analogues. (beilstein-journals.org)
  • You can clarify samples using filters with a large pore size (5 µm or larger) or centrifugation. (cdc.gov)
  • CONCLUSION: There is a strong size-selective difference in particle immobilization. (cdc.gov)
Emulsion2
  • In this study, the potential of centrifugation method in demulsification (emulsion breaking) of coconut milk emulsion is investigated. (scialert.net)
  • Centrifugation produced layers of an aqueous phase (water) on the bottom, an emulsion phase (cream) in the middle and an oil phase on top. (scialert.net)
Ethylene Glycol1
  • Moreover, breathing these particles into the lungs, along with other metals, ethylene glycol and nicotine produces a chemical concoction exhibiting synergistic toxicity, i.e. the toxicity of the whole is higher than the sum of their parts. (wakeup-world.com)
Fluid3
  • All the methods which estimate the unperturbed fluid flow velocity relying on particle suspensions address the same question: How can the fluid velocity be computed measuring the particles trajectory and/or their velocities? (ensam.eu)
  • Tin particles and tin whiskers were identified in cartridge fluid and outer fibers. (wakeup-world.com)
  • In this process, fluid flow is applied parallel to the filter or membrane to capture contaminating particles in the filter layer. (pall.com)
Inks4
  • Upon injection of tattoo inks, particles can be either passively transported via blood and lymph fluids or phagocytized by immune cells and subsequently deposited in regional lymph nodes. (nature.com)
  • We report the synthesis of AgNP@PEDOT:PSS hybrid conductive particles with silver cores and polythiophene shells that can be used to formulate sinter-free inks for printing electronics. (bsz-bw.de)
  • Inks with a solid content of the hybrid particles of 300 mg mL−1 were prepared and deposited on different substrates. (bsz-bw.de)
  • The new particles are components for hybrid inks that become electrically conductive without any chemical or thermal post-deposition treatment. (bsz-bw.de)
Precipitation1
Viral2
  • Enveloped viruses are released from infected cells after coalescence of viral components at cellular membranes and budding of membranes to release particles. (nih.gov)
  • For some negative-strand RNA viruses (e.g., vesicular stomatitis virus and Ebola virus), the viral matrix (M) protein contains all of the information needed for budding, since virus-like particles (VLPs) are efficiently released from cells when the M protein is expressed from cDNA. (nih.gov)
Magnetic5
  • Leukocytes were labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM (Cat. (bdbiosciences.com)
  • Antibody or streptavidin was conjugated to the magnetic particles under optimum conditions, and unconjugated antibody/streptavidin was removed. (bdbiosciences.com)
  • Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM according to the Magnetic Labeling Protocol. (bdbiosciences.com)
  • The PCR products bind to SiMAG-PCR magnetic particles in the presence of chaotropic conditions, and remain tightly bound during washing. (chemicell.com)
  • The magnetic cleanup of PCR products offers greater flexibility than centrifugation- and vacuum-based systems. (chemicell.com)
Poorly1
  • However, the microsized particles could only stay in blood circulation and penetrate poorly into tumor tissues, so that the wide application of the particles for in vivo tumor therapy is limited [ 3 ]. (hindawi.com)
Retention1
  • We hypothesize that the answer lies in the particles by analyzing retention kinetics in et al. (cdc.gov)
Absence2
  • In its absence, mtLSU particles (visualized by cryo-EM at the resolution of 2.6 Å) present disordered RNA domains, partial occupancy of bL36m and bound MALSU1:L0R8F8:mtACP anti-association module, allowing five mtLSU biogenesis intermediates with different intersubunit interface configurations to be placed along the assembly pathway. (nature.com)
  • Immunization of animals with the particles in the absence of adjuvant result in a robust and long-lasting immune response. (bvsalud.org)
Efficiency1
  • 1994). This method eliminates time- consuming repeated extractions with organic solvents and centrifugations without losing the efficiency of recovery. (cdc.gov)
Large4
  • Centrifugation of the fibers produced large pellets containing tin. (wakeup-world.com)
  • These include introduction of the hop pellets into the fermentation/maturation tanks without excessive oxygen pick-up, effective extraction of the aroma compounds into large volumes of beer, removal of the hop particles from the product while minimizing product loss, and efficient CIP of the system. (mbaa.com)
  • We conclude that NPs are much less phagocytized by AMs than large particles but are effec- tively removed from the lung surface into the interstitium. (cdc.gov)
  • Soil samples are first ground and filtered to remove large particles and stones, and then are extracted with acetone-hexane, methanol, acetone, or acetonitrile. (cdc.gov)
Present3
  • After healing, particles are present in the dermis and in the sinusoids of the draining lymph nodes. (nature.com)
  • The present research found that, centrifugation method can enhance the demulsification of oil-in-water (O/W) coconut milk emulsions in a very short time compared to the conventional methods. (scialert.net)
  • In two, CD4+ lymphocytes were present after simple centrifugation and washing. (cdc.gov)
Bind1
  • The kit provides two types of particles, the BD™ CompBead Plus Anti-Mouse Ig, κ particles, which bind any mouse κ light chain-bearing immunoglobulin, and the BD™ CompBead Plus Negative Control (BSA) particles, which have no binding capacity. (bdbiosciences.com)
Separation1
  • Acoustophoresis hich is a separation technology that enables non contact handling of cells and particles in microfluidic systems. (lu.se)
Small2
  • But still I'm going to obtain small plastic particles, if any (spoiler: there will be). (researchgate.net)
  • Anti-RNP antibodies are directed against the U1 particle of an extractable nuclear antigen (ENA) named ribonucleoprotein,[9] which is made up of a complex of proteins and a small nuclear RNA named U1. (medscape.com)
Carbon1
  • Lately, the only study analyzing human lymph nodes in tattooed individuals assessed its contents on carcinogenic polycyclic aromatic hydrocarbons deriving from carbon black particles 13 . (nature.com)
Method1
  • CONCLUSION: The rapid and efficient production of the particles in addition to the robust immune response they generate position this system as an excellent method for the rapid response against emerging diseases. (bvsalud.org)
Temperature3
  • Optional relative humidity and temperature probe integrates RH/T data with particle count data in same data stream. (beckman.com)
  • We find that the agglomeration is enthalpically driven and that, contrary to what one would expect from classical colloid theory, the temperature at which the particles agglomerate increases with increasing solvent chain length. (bsz-bw.de)
  • Proteins contained inside the particles are maintained for over 1 year at ambient temperature, preserving their immunological properties. (bvsalud.org)
Detection1
  • HCT) [3] or the miniature anion exchange parasite detection test carried out on the centrifugation technique (mAECT) [4]-- seropositive persons. (who.int)
Time1
  • For the first time we study how the presence of a wall affects the particle trajectory in oscillatory switching centrifugation dynamics. (ensam.eu)
Effects1
  • The increasing prevalence of tattoos provoked safety concerns with respect to particle distribution and effects inside the human body. (nature.com)
Study1
  • The study authors concluded that "The presence of metal and silicate particles in cartomizer [atomizer/cartridge connecting to the battery] aerosol demonstrates the need for improved quality control in EC design and manufacture and studies on how EC aerosol impacts the health of users and bystanders. (wakeup-world.com)
Separate1
Virus1
  • Trans-complementation of the HIV-1 NL4-3 IN D116N virus with mutant IN molecules restored particle release to levels comparable to WT IN (red), partially restored particle release (gray) or could not restore particle release (blue). (elifesciences.org)
Strategies1
  • We propose some strategies for exploiting the confined oscillatory switching centrifugation for selective particle segregation and for the enhancement of particle interaction events. (ensam.eu)
Types1
  • kinetics for both particle types? (cdc.gov)
Product1
  • See POLYSTYRENE PARTICLE product details for additional information. (spherotech.com)
Distribution1
  • The thermostability conferred by the particle system facilitates the distribution of the vaccines in developing countries or areas with no electricity. (bvsalud.org)