Partial chromosome paints paint your chromosomes. Medical search. Frequent questions

Here we tracked micronucleated chromosomes with live-cell imaging and show that acentric fragments cluster in close spatial proximity throughout mitosis for asymmetric inheritance by a single daughter cell. (nature.com)
The stochastic inheritance of chromosome fragments by both newly formed daughter cells could in part contribute to the alternating DNA copy-number states that are characteristic of chromothripsis 2 . (nature.com)

The main part deals with examples of modern FISH-applications, highlighting unique possibilities of the approach, like the possibility to study individual cells and even individual chromosomes. (frontiersin.org)
As each chromosome contains a single centromere, it remains unclear how acentric fragments derived from shattered chromosomes are inherited between daughter cells during mitosis 6 . (nature.com)

For example, Dr. Harald zur Hausen described an under-condensed appearance of individual chromosomes during mitosis in cell lines derived from 7 different leukemia patients [2] . (prolekarniky.cz)

Finally, ectopic integration of cloned genomic DNA containing ASAR6 causes delayed replication of entire mouse chromosomes. (prolekarniky.cz)

The identification of chromosomes and chromosomal subregions can be a challenging task. (biomedcentral.com)
In addition, numerous reports have described an abnormal chromosomal phenotype affecting single or a few chromosomes in mitotic preparations from tumor-derived and other established cell lines. (prolekarniky.cz)
Furthermore, the under-condensed and late replicating phenotype present in tumor-derived cells occurred only on certain rearranged chromosomes and not on others, suggesting that the under-condensed and late replicating phenotype was associated with certain chromosomal rearrangements [5] . (prolekarniky.cz)

Cytogenetic analysis by G-banding revealed a pericentric inversion of chromosome 2 in 23 out of 25 analyzed metaphases. (biomedcentral.com)
Locus-specific FISH analyses confirmed all deletions and the pericentric inversion of chromosome 2. (biomedcentral.com)
We identified a novel acquired pericentric inversion of chromosome 2, inv(2)(p23.3q24.3), as a sole cytogenetic abnormality together with four additional concurrent submicroscopic deletions in 2p23.3, 2p22.1, and 2q24.3 and 1p13.2. (biomedcentral.com)

MCB probes are established by chromosome microdissection followed by whole genomic DNA amplification. (biomedcentral.com)

The majority of genes on homologous chromosomes replicate synchronously. (prolekarniky.cz)
However, mono-allelically expressed genes such as imprinted genes, allelically excluded genes, and genes on female X chromosomes replicate asynchronously. (prolekarniky.cz)
Disruption of ASAR6 results in delayed replication, delayed mitotic chromosome condensation, and activation of the previously silent alleles of mono-allelic genes on chromosome 6. (prolekarniky.cz)

This study visualizes this phenomenon in a species with ZW sex chromosomes, which evolved independently of XY. (nih.gov)
Generation of MCB probes for chromosomes of other species, useful and required in many cytogenetics research fields, was limited by technical difficulties. (biomedcentral.com)
Here we present a complete workflow for MCB probe generation for those cases and species where chromosome morphology is too challenging to recognize target chromosomes by conventional methods and where WCP probes are not available. (biomedcentral.com)
The present method can be applied for generation of whole or region-specific DNA probes for species, where karyotyping of G-banded chromosomes is challenging due to similar chromosome morphology and/or chromosome banding patterns. (biomedcentral.com)
Besides whole chromosome painting probes (WCP), partial chromosome painting (PCP) probes can also be extremely helpful for the characterization of chromosomes of closely related species and their evolutionary relations to each other. (biomedcentral.com)
However, application of human MCB probes on chromosomes of evolutionarily distant species is challenging, and often not feasible. (biomedcentral.com)
This created the necessity of generating MCB probes for the chromosomes of other species, potentially interesting from the cytogenetic point of view. (biomedcentral.com)

Disruption of ASAR6 leads to the formation of bridged chromosomes, micronuclei, and structural instability of chromosome 6. (prolekarniky.cz)

SignificanceGenes on sex chromosomes (i.e. human chX) are regulated differently in males and females to balance gene expression levels between sexes (XY vs. XX). (nih.gov)
Until recently, MCB probes have only been available for human and some murine chromosomes. (biomedcentral.com)
We have identified a cis -acting locus on human chromosome 6 that controls this replication-timing program. (prolekarniky.cz)

The individual under-condensed chromosomes synthesized DNA after the normally condensed chromosomes had finished replication, indicating that the under-condensed chromosomes were extremely late replicating, with DNA synthesis extending into the G2 phase. (prolekarniky.cz)

Previously proposed protocols suggested G-banding staining or preliminary FISH with whole chromosome paints (WCP) as methods to identify the chromosome of interest. (biomedcentral.com)