The following YALI0_B22968g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
These sequences represent the protein coding region of the YALI0_B22968g cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
The clones bear about 80% sequence homology with the respective rat cDNAs in the sequenced regions and hybridize with the rat clones in 5 X SSC at 55°C. The ovine LHβ clone has an insert of about 650 bp and selects an RNA of about 750 bases in a northern blot. (ias.ac.in)
A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. (wiley.com)
Sequence analysis revealed a partial cDNA clone of 643 bp with a start codon and with 96% and 93% homology with peanut allergens Ara h 4 and Ara h 3 cDNA clones, respectively. (usda.gov)
The absence of genomic resources renders the search for paralogous cuttlefish genes dependent upon the presence of highly conserved protein motifs within genes of interest, thus we used a degenerate primer strategy to isolate partial coding sequence from the conserved domains specific to these families. (europa.eu)
We isolated partial coding sequence for a retinoid X receptor (RXR) receptor (Sof-RXR : JN101950) and an aldehyde dehydrogenase (ALDH) (Sof-ALDH : JN101949) homologue. (europa.eu)
Canine ortholog cDNA sequence was cloned and verified using RPE/choroid 5′- and 3′-RACE. (upenn.edu)
Can i consider an sequence to be full-length cDNA, if the ORF in the sequence aligns with ~99%-100% identity, with 100% coverage to the reference protein? (biostars.org)
Here, we present the completed coding sequence and genomic organization of the previously published partial cDNA sequence of MUC17. (nih.gov)
Sequences5
We have constructed a partial library of Y chromosome derived DNA sequences of bovine origin in Escherichia coli . (ias.ac.in)
We used PCR cloning to obtain partial cDNA sequences of mTOR, Rheb, AKT, S6K and AMPK. (awi.de)
Could i please know, if there are any tools/scripts available which can run locally to predict full-length cDNA sequences. (biostars.org)
This method works when you have complete sequences, if u have partial sequences u get partial CDS (naturally! (biostars.org)
Rapid amplification of cDNA ends with PCR, sequences from the Human Genome databases, and in vitro transcription/translational assays were used for these analyses. (nih.gov)
Clone1
Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. (wiley.com)
Cloning4
Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells. (nih.gov)
Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. (wiley.com)
Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. (embl-heidelberg.de)
We report here the cloning and sequencing of the cDNA, purification, steady state kinetic analysis, and truncation mapping studies of the human 5-aminoimidazole- 4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase). (embl-heidelberg.de)
Protein2
The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. (embl-heidelberg.de)
LOCUS FM865412 2966 bp RNA linear VRL 26-JUL-2016 DEFINITION Norovirus Hu/GIV.1/Italy/980/2007/ITA partial RNA polymerase (ORF1), capsid protein (0RF2) and partial minor structural protein (ORF3), genomic RNA. (cdc.gov)
Genomic1
Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA. (ucsc.edu)
Antibodies1
Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
Clones2
We have isolated cDNA clones for ovine α -subunit and LHβ. (ias.ac.in)
The identification of these clones was confirmed by partial sequencing. (ias.ac.in)
Oligonucleotides1
Two oligonucleotides were selected as probes to screen a peanut Lambda gt 11 cDNA library. (usda.gov)
Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy. (embl-heidelberg.de)
Mutant2
In case of in vivo expression analysis, cells transfected with this mutant cDNA showed no evidence of restored T2 activity. (jci.org)
To assess their rescue ability, transgenes were placed into two different Gap1 mutant backgrounds: the partial loss-of-function allele Gap1 S1-11 and the complete loss-of-function allele Gap1 B2 (Gaul, 1992). (sdbonline.org)
Arch1
REFERENCE 1 AUTHORS La Rosa,G., Pourshaban,M., Iaconelli,M. and Muscillo,M. TITLE Detection of genogroup IV noroviruses in environmental and clinical samples and partial sequencing through rapid amplification of cDNA ends JOURNAL Arch. (cdc.gov)
Fragment of partial1
G633-9Insert Size:1 kb fragment of partial ORF1ab and partial S (28259 - 29258) SARS-CoV-2 sequenceStorage Condition:Store at -20°C. Avoid cycles of freezing and thawing. (topsan.org)
Plasmid1
A cDNA library of ovine pituitary DNA in plasmid pBR322 has been constructed by conventional methods with certain modifications. (ias.ac.in)
Exon2
The paternal allele was associated with exon 8 skipping at the cDNA level. (jci.org)
In vivo splicing experiments revealed that the exonic mutation caused partial skipping of exon 8. (jci.org)
Polymerase2
Sanger sequencing of the patient's cDNA after nested polymerase chain reaction showed that the patient had both a normal transcript containing. (lu.se)
The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liverRNA by reverse transcription and the polymerase chain reaction (PCR). (embl-heidelberg.de)
Human1
The human purH cDNA is 1776 base pairs in length encoding for a 591-amino acid polypeptic (Mr = 64,425). (embl-heidelberg.de)
Chromosome1
The remaining cases are due translocation, mosaicism and partial trisomy of the chromosome 21. (bvsalud.org)
Differences2
it has two internal Pst I sites and thus shows restriction-based differences from rat α -subunit cDNA, which does not have any Pst I site. (ias.ac.in)
[ 11 ] reported that no phenotypic differences were observed among patients with partial deletion, complete deletion, and nondeletion, supporting a haploinsufficiency model for Rubinstein-Taybi syndrome. (medscape.com)
Library1
The library was screened using partial cDNAs for rat α -subunit and LHβ. (ias.ac.in)
Complete1
Gap1 transgenes containing only the catalytic domain ( sevGapM ) fail to rescue either the partial or complete loss-of-function phenotype of Gap1 . (sdbonline.org)
Region2
A region at the N terminus of the mouse cDNA contains three tandem repeats homologous to MAM domains. (embl.de)
The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide (Mr = 64 200). (embl-heidelberg.de)
Length1
CBP spans approximately 150 kb with 31 exons, and its cDNA is 9 kb in length. (medscape.com)
Specific1
Stimulation of CysLTR2 by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. (rndsystems.com)
Domains2
We have now cloned a cDNA for mouse zonadhesin (16.4 kb), and it demonstrates a large species variation in the numbers and arrangements of domains. (embl.de)
There are 20 partial D-domains, found as tandem repeats, inserted between two of the four full D-domains and an additional partial D-domain. (embl.de)