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  • genes
  • The early genes E6 and E7 of human papillomavirus type 16 (HPV16) are consistently and exclusively expressed in HPV16-induced cancer lesions and play major roles in the development and maintenance of the malignant phenotype. (aacrjournals.org)
  • We show that E6 and E7 decrease expression of TGF-β2 mRNA and alter expression of multiple TGF-β-responsive genes involved in cell cycle regulation, apoptosis, and tissue remodeling. (aacrjournals.org)
  • His accomplishments include the discovery that pRB is targeted by human papillomavirus E7 proteins, the finding that pRB and p107/p130 are required for the regulation of different sets of E2F-target genes, and the identification and analysis of Drosophila E2F, DP and RB family members. (degruyter.com)
  • epithelial
  • E6 can enhance telomerase activity through an unknown mechanism ( 29 , 57 ), whereas E7 inhibits a p16 ink4A -dependent pathway that limits cellular proliferation in epithelial cells ( 28 ). (asm.org)
  • Abdelsayd RA (1991) Study of human papillomavirus in oral epithelial dysplasia and epidermoid carcinoma in the absence of tobacco and alcohol use. (springer.com)
  • carcinomas
  • Transgenic mice, which constitutively and specifically express the E7 HPV16 gene in the thyroid epithelium, rapidly develop thyroid goiters and, after several months, thyroid carcinomas. (aacrjournals.org)
  • vaccine
  • Because this protein is a good example of a tumor-associated antigen, we have used E7 as a model antigen to test the potential of an experimental vaccine as an immunotherapeutic approach. (aacrjournals.org)
  • In this study, we used a murine E7-expressing tumor model (TC1 cells) to assess effects of an E7-based vaccine on tumor growth. (aacrjournals.org)
  • Two responding patients generated long-term T-cell immunity toward the vaccine antigen and E7 as well as a weak H. influenzae protein D (PD)-directed CD4(+) response. (ac.be)
  • Conclusions: The encouraging results obtained from this study performed on a limited number of subjects justify further analysis of the efficacy of the PD-E7/AS02B vaccine in CIN patients. (ac.be)
  • Algae have been shown to accumulate and properly fold several vaccine antigens, and efforts are underway to create recombinant algal fusion proteins that can enhance antigenicity for effective orally delivered vaccines. (frontiersin.org)
  • immunogenicity
  • In the present study, we tested the immunogenicity of a fusion protein (PD-E7) comprising a mutated HPV-16 E7 linked to the first 108 amino acids of Haemophilus influenzae protein D, formulated in the GlaxoSmithKline Biologicals adjuvant AS02B, in patients bearing oncogenic HPV-positive cervical intraepithelial neoplasia (CIN). (ac.be)
  • The objectives of the study were to examine the clinical and environmental safety and immunogenicity in the first clinical trial of a live recombinant vaccinia virus expressing the E6 and E7 proteins of HPV 16 and 18 (TA-HPV). (openrepository.com)
  • Cellular
  • Complex formation between E6 and p53 requires an additional cellular factor, designated E6-AP (E6-associated protein), which has a native and subunit molecular mass of approximately 100 kDa. (pubmedcentralcanada.ca)
  • responses
  • Results: Some patients had preexisting systemic IFN-gamma CD4(+) (1/10) and CD8(+) (5/10) responses to PD-E7. (ac.be)
  • Five vaccinated patients (71%) showed significantly increased IFN-gamma CD8(+) cell responses upon PD-E7 stimulation. (ac.be)
  • The patients were vaccinated with a single dose of TA-HPV and kept in strict isolation to monitor local and systemic side-effects, environmental spread, and anti-E6/E7 immune responses. (openrepository.com)
  • phenotype
  • Most of these changes were qualitatively similar in cells immortalized by E6, E7, or E6/7 expression, indicating convergence on a common phenotype, but fifteen proteins (~2%) were outliers in this regulatory pattern. (biomedcentral.com)
  • immune
  • RATIONALE: Vaccines made from human papillomavirus may help the body build an effective immune response to kill HIV cells. (bioportfolio.com)
  • These approaches have the potential to revolutionize the way subunit vaccines are made and delivered - from costly parenteral administration of purified protein, to an inexpensive oral algae tablet with effective mucosal and systemic immune reactivity. (frontiersin.org)
  • expression
  • In contrast, E6 and E7 did not alter expression of TGF-β1 and TGF-β3. (aacrjournals.org)
  • This fact is supported by the finding that most HPV-positive cancer cells maintain the expression of E6 and E7 ( 4 , 52 ). (asm.org)
  • Proteomic profiling was used to compare expression levels for 741 discrete protein features. (biomedcentral.com)
  • cells
  • By inhibiting these and other regulatory mechanisms, HPV E6 and E7 allow for the accumulation of genetic mutations and the survival of mutated cells ( 8 , 42 , 61 ). (asm.org)