Lipase-catalyzed synthesis of sugar esters, as lactulose palmitate, requires harsh conditions, making it necessary to immobilize the enzyme. (pucv.cl)
Extending this work, immobilised lipases have novel capabilities in the synthesis of sugar esters. (researchgate.net)
Rugosa3
The hydrolysis of p-nitrophenyl palmitate by six different lipases: lipase from porcine pancreas (PR), lipase from Candida rugosa (CR), Amano lipase PS, from Burkholderia cepacia (AM), lipase from Rhizopus niveus (RN), lipase acrylic resin from Candida antartica (ARC), lipase B Candida antartica immobilized on Immobead 150, recombinant from Aspergillus oryzae (CALB), were tested in five NADESs. (um.edu.my)
Spent coffee grounds were treated to obtain suitable carriers for both physical and covalent immobilization of Candida rugosa lipase. (uniroma1.it)
In the present study, immobilisation of Candida rugosa (CRL) lipase onto amino-activated mica is performed via covalent bonding (namely Amino-CRL) and the cross-linking of lipases into nano-reactors through physical adsorption (namely NER-CRL). (researchgate.net)
Catalytic4
The catalytic potential of the immobilized lipase was evaluated in hydrolytic reactions using p-nitrophenol palmitate as substrate. (eurekaselect.com)
The kinetics investigation confirmed the higher catalytic efficiency (kcat/Km) of lipases in the 40 % of (NADES3) and compared with the aqueous form. (um.edu.my)
Methanol-resistant lipases with high catalytic activity are the first choice for practical production. (biomedcentral.com)
Therefore, improving the specific tolerance and catalytic efficiency of the enzyme is key to further expanding the application of lipase. (biomedcentral.com)
This study presents the potential role of natural deep eutectic solvents (NADESs) in a lipase-catalyzed hydrolysis reaction as both a co-solvent in an aqueous solution and as a main solvent. (um.edu.my)
This solid biocatalyst also preserved 100 % of its activity after four recycles in p-nitrophenyl palmitate hydrolysis in hexane medium and permitted an almost 60 % conversion of milk fats in fatty acids after 18 h of reaction, maintaining this value for three reuses. (uniroma1.it)
Immobilization4
Results: Immobilization enhanced the lipase activity and the structure of the nanogels made significant difference in enhancing their activity as the lipase immobilized on poly(Acryl azide)-cl-divinylbenzene nanogel exhibited the higher activity than the lipase immobilized on the precursor nanogel. (eurekaselect.com)
Each immobilization method was investigated to obtain the best insoluble biocatalyst immobilized activity, efficiency, and recovery. (uniroma1.it)
Therefore, a study was conducted to evaluate the effect of different chemical surfaces of hierarchical meso-macroporous silica in the immobilization of two lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (pucv.cl)
Therefore, screening of different chemical surfaces on porous silica used as supports for lipase immobilization allowed obtaining active and stable biocatalyst to be employed in the novel synthesis of lactulose palmitate. (pucv.cl)
Enzyme2
The optimized immobilized biocatalyst obtained by adsorption mechanism showed up to 33 U∙g-1 of immobilized enzyme activity, and a higher pH, thermal, and storage stability than the free lipase. (uniroma1.it)
This study provides an effective approach to improve the design of the enzyme and the properties of lipase mutants, thereby rendering them suitable for industrial biomass conversion. (biomedcentral.com)
Stability2
The immobilized lipase exhibited thermal and pH stability, which is not possible with the free lipase. (eurekaselect.com)
The results indicated that the environmental stability and antioxidant activity of oil droplets coated with HA-PG10-C18 surpassed those coated with PG10-C18 due to the formation of a denser interfacial layer that blocked metal ions, oxygen, and lipase. (bvsalud.org)
Acetone1
Lactulose palmitate synthesis was carried out in acetone medium (with 4% of equilibrium moisture) at 40°C obtaining palmitic acid conversions higher than 20% for all biocatalysts, being the highest of those obtained with OGS-AsL and OS-PsL. (pucv.cl)
Acid1
With a very high molar proportion of fatty acid to saccharide when compared to the other studies, the authors suggested that increasing the proportion of fatty acid could negatively affect the lipase conformation due to an acidification of the reaction medium. (researchgate.net)
Protein1
Rhizomucor miehei lipase (RML) is a single-chain α/β-type protein that is widely used in biodiesel preparation. (biomedcentral.com)
Activity4
The activity assay of the immobilized lipase was carried out by the standard assay method. (eurekaselect.com)
The immobilized lipase exhibited good activity, storability and reusability. (eurekaselect.com)
From a practical perspective, the activity, thermostability, and methanol tolerance of lipase are the main reasons that hinder its effective application in the biodiesel field, resulting in prolonged reaction times and increased production costs [ 5 ]. (biomedcentral.com)
The temperature range generally oscillates between 40 °C and 60 °C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
C, a characteristic interval of the immobilized form, which usually guarantees better conditions because it is more stable than the free form. (researchgate.net)
Position2
All of the reported lipases primarily catalyzed esterification of lactose at the C6hydroxyl position (Table 1). (researchgate.net)
Most palmitate and stearate were bound at the sn-1 position of 16:0- and 18:0-PC, respectively, and 18:0-PC was found in type I and IIa fibers. (bvsalud.org)
Supports1
The well characterized precursor nanogel and its functional derivatives were used as supports to immobilize lipase (triacyl glycerin). (eurekaselect.com)
Active1
This method was applied to the conjugation of biotin and β-linked galactose residues to yield an enzymatically active thermophilic lipase, which revealed specific binding to Erythrina cristagalli lectin by SPR binding studies. (beilstein-journals.org)
Surfactant1
Here we describe the synthesis, properties and enzyme activity of self-contained reactive biofluids based on solvent-free melts of lipase-polymer surfactant nanoconjugates. (nature.com)
Lanuginosus1
Here we show for the first time that biologically active solvent-free liquid proteins can be prepared using lipases from the mesophile Rhizomucor miehei (RML) and thermophile Thermomyces lanuginosus (TLL). (nature.com)
Solvent2
Lipase-mediated catalysis has been undertaken with reagent-based solvents and lyophilized powders, but there are no examples of molecularly dispersed enzymes that catalyse reactions at sub-solvation levels within solvent-free melts. (nature.com)
Desiccated substrates in liquid (p-nitrophenyl butyrate) or solid (p-nitrophenyl palmitate) form can be mixed or solubilized, respectively, into the enzyme biofluids, and hydrolysed in the solvent-free state. (nature.com)
Esters1
In aqueous environments, lipases catalyse the hydrolysis of fatty acid esters by a two-step mechanism that involves nucleophilic attack by a serine residue located within the active site to produce an acylated enzyme and alcohol product, followed by nucleophilic addition of a water molecule to liberate the fatty acid 1 . (nature.com)
Synthesis1
Additionally, Dr. Vadgama has been involved in projects involving the resolution of D/L Menthol through lipase-based methods, as well as the synthesis of green and Clean Methyl ricinoleate and extractive microbial oil. (kolabtree.com)
Substrate1
The lipases comprise an active site based around a serine-histidine-aspartate triad (Ser144-His257-Asp203 for RML, Ser146-His258-Asp201 for TLL) with substrate access regulated by conformational changes involving a helical 'lid' motif ( Fig. 1a ), and which together facilitate nucleophilic attack on the fatty acid ester substrate to produce the acylated enzyme and alcohol product ( Fig. 1b ). (nature.com)
Fatty1
These projects have focused on utilizing lipase-based techniques for the production of fatty acids such as Erucic acid, Alpha-Linolenic acid, and Ricinoleic acid. (kolabtree.com)