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  • species
  • The ten GGA macrochromosome paints unequivocally recognize 14 to 16 hybridizing regions delineating the conserved chromosomal segments for the respective chicken macrochromosomes in these representative parrot species. (kent.ac.uk)
  • Among the larger GGA macrochromosomes (1-5), chromosomes 1 and 4 are conserved on two chromosomes in all three species. (kent.ac.uk)
  • In contrast, the smaller GGA macrochromosomes 6, 7 and 8 displayed a complex hybridization pattern: two or three of these macrochromosomes were found to be contiguously arranged on a single chromosome in all three parrot species. (kent.ac.uk)
  • translocation
  • Although two-dimensional (2-D) microscopic images are typically used in FISH signal analysis, we present a case where the translocation occurs in the depth direction where two probed FISH signals are overlapped in the projected image plane. (spiedigitallibrary.org)
  • Objective: The Philadelphia (Ph) chromosome, consisting of the t(9;22)(q34;q11) translocation, is observed in--90% of patients with chronic myeloid leukemia (CML). (biomedsearch.com)
  • The translocation leads to fusion of the proto-oncogene Abelson (ABL) and a particular DNA sequence known as breakpoint cluster region (BCR), thereby giving rise to 2 new chimeric genes 5' ABL-3' BCR on the derivative chromosome 9 and 5' BCR-3' ABL on the derivative chromosome 22. (biomedsearch.com)
  • nuclei
  • Kinetochores divide, and chromosomes migrate to the poles (anaphase 2), followed by telophase 2, in which haploid nuclei form. (umanitoba.ca)
  • Nuclei display a Rabl configuration for only ∼2 h after mitosis, and with further progression of G1-phase can establish heterochromatic interactions between distal and proximal parts of the chromosome arm. (rupress.org)
  • Isolated single chromosomal regions can be directly amplified and labeled by the Degenerate Oligonucleotide-Primed Polymerase Chain Reaction (DOP-PCR) and subsequently hybridized to chromosomes and interphasic nuclei. (biomedcentral.com)
  • Cytogenetic
  • In maize ( Zea mays L., 2 n = 20) pachytene chromosomes have been used extensively for karyotyping and cytogenetic analyses. (pnas.org)
  • By examining the chromosomal homologies defined by chromosome painting among two representatives of the subfamily Glossophaginae ( Glossophaga soricina and Anoura cultrata ) and one species from the subfamily Lonchophyllinae ( Lonchophylla concava ), we found chromosomal correspondence in regions not previously detected by other comparative cytogenetic techniques. (biomedcentral.com)
  • Chromosome nanolithography, with a resolution beyond the resolution limit of light microscopy, could be useful to the construction of chromosome band libraries and to the molecular cytogenetic mapping related to the investigation of genetic diseases. (biomedcentral.com)
  • centromere
  • The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper Eyprepocnemis plorans derived intraspecifically from the X chromosome. (karger.com)
  • Centromere positions on the majority of orthologous chromosomes are different in these two species. (springer.com)
  • fluorescent
  • These probes, coupled with changes in the chromosome-preparation procedure that improve fluorescent signal detection, allowed the development of a FISH karyotyping method that is effective on all tested maize lines. (pnas.org)
  • derivative
  • Here we demonstrate the power of in-nucleus Hi-C [ 14 ], a derivative of the chromosome conformation capture (3C) technique [ 15 ], to detect both known and novel, balanced and unbalanced chromosomal rearrangements from cell lines and human tumour samples. (biomedcentral.com)
  • consist
  • These species have typical avian karyotypes that consist of several pairs of relatively large macrochromosomes (chromosomes 1-10 and a pair of sex chromosomes, ZW in females and ZZ in males) and numerous tiny microchromosomes. (springer.com)
  • sequences
  • The procedure uses tandemly repeated DNA sequences to generate a distinctive banding pattern for each of the 10 chromosomes. (pnas.org)
  • Pairing can only take place at the PAR, because only those sequences are homologous, between the X and Y chromosomes. (umanitoba.ca)
  • Our results have confirmed that X and B chromosomes share many DNA sequences between them and with most of the autosomes, especially at locations where the satDNA and rDNA reside, in consistency with previous information. (karger.com)
  • aberrant
  • A limited number of mammal species have, however, evolved to escape convention and present aberrant sex chromosome complements. (sun.ac.za)
  • hypothesis
  • One simple hypothesis is that by bringing all chromosome ends together into a small bundle, it is easier for the homology-searching mechanism to bring both homologues of each chromosome together. (umanitoba.ca)
  • This supports the hypothesis of an intraspecific origin of B chromosomes in E. plorans . (karger.com)
  • human
  • Cytocell offers a comprehensive range of human Whole Chromosome Painting probes available in the Aquarius ® liquid format. (cytocell.com)
  • confirmation of results obtained from M-FISH and SKY testing and may be of particular interest to those studying mutagenesis of human chromosomes as a result of exposure to genotoxic agents. (cytocell.com)
  • CytoTest FISH probes are manufactured with genomic DNA obtained either from microdissected human chromosomes or cloned DNA fragments, depending on the probe type. (cambio.co.uk)
  • Human chromosomes at pachytene. (umanitoba.ca)
  • We proposed the corresponding human chromosomal segments for chromosomes of the investigated species and found two syntenic associations shared by G. soricina and A. cultrata . (biomedcentral.com)
  • A genetic test is the analysis of human deoxyribonucleic acid (DNA), ribonucleic acid (RNA), chromosomes, and proteins to detect heritable disease-related genotypes, mutations, phenotypes, or karyotypes (standard pictures of the chromosomes in a cell) for the purposes of diagnosis, treatment, and other clinical decision making. (encyclopedia.com)
  • This study partially fills the noticeable gaps between our knowledge of the elementary DNA-protamine structure and the higher-order chromosome packing in human sperm cells. (biologists.org)
  • Here we describe the use of Hi-C as a tool for detection of both balanced and unbalanced chromosomal rearrangements in primary human tumour samples, with the potential to define chromosome breakpoints to bp resolution. (biomedcentral.com)
  • Although Hi-C has previously been used to detect and confirm chromosome rearrangements in cell lines [ 16 - 18 ], it has not, until now, been used on human primary tumour material or to detect copy number information. (biomedcentral.com)
  • FISH
  • In our hands, Cytocell FISH probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. (cytocell.com)
  • The versatility and resolution of FISH depends critically on the probe set used. (springer.com)
  • However, without the precise level of blocking with unlabeled repetitive DNA, the FISH procedure tends to label all chromosomes nonspecifically because of the presence of retrotransposons in the probe. (pnas.org)
  • Karyotyping was performed and the presence of chromosome 8 aneuploidy was tested with the FISH technique. (omicsonline.org)
  • relatively
  • Although chromosome analysis is routinely used in diagnosing disease, predicting its prognosis and deciding the optimal treatment plan, the conventional chromosome banding analysis (karyotyping) has limitations due to its relatively lower resolution or poor contrast of the chromosome G- or Q-banging patterns. (spiedigitallibrary.org)
  • Tetrasomy, pentasomy, and hexasomy of chromosome 8 are relatively rare compared to trisomy 8, which was found to be associated with an unfavorable disease prognosis [ 5 ]. (omicsonline.org)
  • In addition, the conventional methods are not suitable for single chromosome analysis, because of the relatively big size of the microneedles. (biomedcentral.com)
  • detect
  • This study aimed to detect the prevalence of chromosome 8 aneuploidy in Egyptian AML patients and analyse its prognostic impact. (omicsonline.org)
  • This study aimed to detect the prevalence of aneuploidy in chromosome 8 among Egyptian patients who presented with acute myeloid leukemia and to assess the impact of chromosome 8 aneuploidy on the clinical and haematological presentation of the studied cases. (omicsonline.org)
  • sperm
  • Based on acquired data and data that had already been published, we propose a consensus model of DNA compacting in sperm, starting with the protamine toroids as an elementary unit followed by the well-defined higher-order chromosome architecture. (biologists.org)