KinaseInositol phosphateReceptorHydrolysisPI4PBindsHippocampal slicesAgonist-inducedPIP3MannoseProteinsAccumulationPhospholipidCardiolipinPmolPathwayDomainFactorTitleLevelsAnalysisDomainsPhosphorylationPhosphoinositideBiosynthetic processInositolProteinCatalyticMetabolismEnzyme calledLipidSignificantlyVps34KinasesMembraneSearchAnnotationsProcessVolumeFormationArticles
Kinase17
- Scholars@Duke publication: Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in Schizosaccharomyces pombe. (duke.edu)
- The ste12+ gene of Schizosaccharomyces pombe codes for a phosphatidylinositol (PI) 3-phosphate 5'-kinase, which is required for efficient mating. (duke.edu)
- Phosphatidylinositol 4-phosphate 5-kinase type I g (PIPKIγ90) regulates cell migration, invasion, and metastasis. (arizona.edu)
- Oomycetes possess a family of novel proteins called GPCR-PIPKs (GKs) that are composed of a seven-transmembrane spanning (7-TM) domain fused to a phosphatidylinositol phosphate kinase (PIPK) domain. (wur.nl)
- A previous screen of a human kinase and phosphatase shRNA library to select genes that mediate arsenite induction of spindle abnormalities resulted in the identification of phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PIP4KIIγ), a phosphatidylinositol 4,5-bisphosphate (PIP2)-synthesizing enzyme. (biomedcentral.com)
- The PH domain of PKB binds phosphatidylinositol 3,4,5-trisphosphate translocating PKB to the plasma membrane for activation by 3-phosphoinositide-dependent protein kinase 1. (ox.ac.uk)
- 2009 122 3837 3850 4 Loijens J. C. Boronenkov I. V. Parker G. J. Anderson R. A. The phosphatidylinositol 4-phosphate 5-kinase family Adv. Enzyme Regul. (silverchair.com)
- Herein, we report that ablation of B cell antigen receptor ubiquitination in vivo uncouples the receptor from CD19 phosphorylation and phosphatidylinositol 3-kinase (PI3K) signals. (ox.ac.uk)
- Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) enzyme family member. (utoronto.ca)
- Recent work from the authors' laboratory has established the role of type Iγ phosphatidylinositol 4-phosphate 5-kinase (PIPKIγ) in the trafficking of E-cadherin by studying the surface accessibility of E-cadherin in endocytosis and recycling assays. (elsevierpure.com)
- in yeast, overexpression of a phosphatidylinositol 3-kinase (VPS34) specifically increases phosphatidylinositol 3-phosphate (PI3P). (elsevierpure.com)
- Here we provide biochemical and cellular evidence that artemisinins are potent inhibitors of Plasmodium falciparum phosphatidylinositol-3-kinase (PfPI3K), revealing an unexpected mechanism of action. (nature.com)
- Polyubiquitination of PfPI3K and its binding to PfKelch13 were reduced by the PfKelch13 mutation, which limited proteolysis of PfPI3K and thus increased levels of the kinase, as well as its lipid product phosphatidylinositol-3-phosphate (PI3P). (nature.com)
- The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). (colinsbraincancer.com)
- Type III PI4Ks are structurally linked to phosphatidylinositol 3-kinases (PI3K) because the C-terminal catalytic site and a helical lipid kinase exclusive site (LKU) both are extremely conserved and both lipid kinase family members share a level of sensitivity to wortmannin (16 25 Besides that small is well known about structural determinants of PI4KIIIα playing a job in enzymatic function. (colinsbraincancer.com)
- One of these is activation of phosphatidylinositol 3-kinase (PI-3K), which results in the generation of a membrane-restricted second messenger, polyphosphatidylinositides containing a 3'-phosphate. (northwestern.edu)
- We investigated the role of phosphatidylinositol-4-phosphate 5-kinase alpha (PIP5Kα), a key upstream factor of PI3K/AKT, and the therapeutic effect of PIP5Kα inhibitor on subtypes of BC. (lu.se)
Inositol phosphate4
- Bz(1,2,3,4)P4 is an example of a simple inositol phosphate surrogate crystallized in a protein, and this approach could be applied to design modulators of inositol polyphosphate binding proteins. (ox.ac.uk)
- We suggest the physiological ligand of the kindlin-1 PH domain is most likely not an inositol phosphate but another phosphorylated species. (ox.ac.uk)
- Exposure to ET-1 (15 min) resulted in concentration-dependent and homologous desensitization (40%) of the inositol phosphate response triggered by ET-1. (aspetjournals.org)
- There was virtually no recovery of ET-1-mediated inositol phosphate responses in the desensitized tissue even after 180 min of incubation. (aspetjournals.org)
Receptor6
- These data suggest that phorbol ester receptor-mediated events may be negatively coupled to agonist-induced phosphatidylinositol hydrolysis. (wustl.edu)
- Endothelin receptor type A signals both the accumulation of inositol phosphates and the inhibition of cyclic AMP generation in rat myometrium: stimulation and desensitization. (aspetjournals.org)
- These signals are necessary and sufficient for accumulating phosphatidylinositol (3,4,5)-trisphosphate (PIP3) on B cell receptor-containing early endosomes and proper sorting into the MHC class II antigen-presenting compartment (MIIC). (ox.ac.uk)
- Our results suggest that the measurement of agonist-induced [ 3 H]-myo-inositol-1-phosphate accumulation, in the presence of lithium, represents a sensitive method for studying a number of receptor-mediated events in brain. (elsevierpure.com)
- Paul, Steven M. / Characterization of neurotransmitter receptor-mediated phosphatidylinositol hydrolysis in the rat hippocampus . (elsevierpure.com)
- These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. (rupress.org)
Hydrolysis4
- The stimulation of phosphatidylinositol hydrolysis by various neurotransmitter agonists was investigated in rat hippocampal slices using a rapid and sensitive radioisotopic method. (elsevierpure.com)
- The hydrolysis of phosphatidylinositol in hippocampal slices is induced by carbachol, serotonin, norepinephrine and phenylephrine. (elsevierpure.com)
- The concentration response curve for serotonin-induced phosphatidylinositol hydrolysis is bimodal and the effect is blocked by metergoline, but not mianserin, indicating that the effect of serotonin in the hippocampus may be mediated by 5HT l receptors. (elsevierpure.com)
- However, in human follicular cells and in rat FRTL-5 cells, TSH can also stimulate the β-isoforms of PLC that catalyzes the hydrolysis of phosphatidyl-inositol 4,5-phosphate, yielding the second messengers DAG and inositol 1,4,5-phosphate facil. (endocrine-abstracts.org)
PI4P1
- At the molecular level, Osh6 transports phosphatidylserine (PS) and phosphatidylinositol-4-phosphate (PI4P) between the endoplasmic reticulum (ER) and the plasma membrane (PM). To decipher how an ER-PM working protein controls vacuolar morphology, we tested genetic interactions between OSH6 and DRS2 , whose protein flips PS from the lumen to the cytosolic side of the Golgi, the organelle between ER and vacuoles in many pathways. (microbialcell.com)
Binds3
- Phosphatidylinositol 4,5-bisphosphate (PI4,5P2, hereafter referred to as PIP2) either binds to intracellular proteins and directly modulates their subcellular localization and activity or acts as a precursor for the generation of other second messengers. (biomedcentral.com)
- The novel achiral analogue benzene 1,2,3,4-tetrakisphosphate (Bz(1,2,3,4)P4) possesses phosphate regiochemistry different from that of Ins(1,3,4,5)P4 and surprisingly binds with similar affinity as the natural headgroup. (ox.ac.uk)
- A fifth subunit, p40 phox , plays an important role in phagocytosis-induced superoxide production via a phox homology (PX) domain that binds to phosphatidylinositol 3-phosphate (PtdIns(3)P). We report the first case of autosomal recessive mutations in NCF4, the gene encoding p40 phox , in a boy who presented with granulomatous colitis. (wustl.edu)
Hippocampal slices2
- In rat hippocampal slices, carbachol and norepinephrine induce an accumulation of [ 3 H]-inositol-1-phosphate which is markedly amplified in the presence of lithium. (wustl.edu)
- The tumor-promoting agents phorbol 12, 13-dibutyrate (PDB) and 4β phorbol, 12β-myristate, 13 α-acetate (PMA) have no effect on [ 3 H] inositol-1-phophate accumulation alone, but when preincubated with hippocampal slices significantly inhibit the accumulation of [ 3 H]-inositol-1-phosphate induced by carbachol and norepinephrine. (wustl.edu)
Agonist-induced1
- The agonist-induced accumulation of [ -3 H]-myo-inositol-1-phosphate was dependent on tissue, lithium, [ 3 H]-myo-inositol concentration, as well as incubation time. (elsevierpure.com)
PIP31
- Phosphatase and tensin homologue (PTEN) is an antagonist of the Akt pathway through its phosphatase activity against phosphatidylinositol-3,4,5-phosphate (PIP3). (aston.ac.uk)
Mannose1
- 2006. PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria. . (umass.edu)
Proteins1
- Phosphatidylinositol 4,5-biphosphate (PIP 2 ) modulates the function of numerous ion transporters and channels, as well as cell signaling and cytoskeletal proteins. (elsevierpure.com)
Accumulation2
- In estradiol-dominated rat myometrium, endothelin (ET)-1 caused contraction and increased the accumulation of [3H]inositol phosphates (EC50 = 70 nM), with the sequential generation of inositol trisphosphate, inositol bisphosphate, and inositol monophosphate. (aspetjournals.org)
- Slices were preincubated with [ 3 H]-myo-inositol and the accumulation of [ 3 H]-myo-inositol-1-phosphate induced by various agonists was determined in the presence of 10 mM lithium. (elsevierpure.com)
Phospholipid1
- Brush-border membranes from dexamethasone-treated neonates had a decrease in sphingomyelin and an increase in phosphatidylcholine and phosphatidylinositol content but no change in cholesterol or total phospholipid content. (elsevierpure.com)
Cardiolipin1
- The HF diet group had significantly increased abundance of triglycerides and phosphatidylinositol lipids, as well as decreased lysophosphatidic lipids and cardiolipin. (cdc.gov)
Pmol1
- The major anionic head groups can be quantified in single runs with practical detection limits of about 100 pmol, and the D3 isoforms of phosphatidylinositol phosphate (PIP) and PIP 2 are detected as shoulder peaks. (elsevierpure.com)
Pathway1
- Ceramide-1-phosphate was found at higher abundance in the regular (REG) diet WF-exposed group which has been shown to regulate the eicosanoid pathway involved in pro-inflammatory response. (cdc.gov)
Domain1
- Structural comparison with other PH domains suggests that the phosphate binding pocket in the kindlin-1 PH domain is more occluded than in kindlins-2 and -3 due to its salt bridge. (ox.ac.uk)
Factor1
- The present study examined whether glucocorticoids are also a potentially important factor in the maturational decrease in proximal tubule phosphate transport. (elsevierpure.com)
Title1
- Phosphatidylinositol phosphate may refer to: Phosphatidylinositol 3-phosphate, also known as PI(3)P Phosphatidylinositol 4-phosphate, also known as PI(4)P Phosphatidylinositol 5-phosphate, also known as PI(5)P This disambiguation page lists articles associated with the title Phosphatidylinositol phosphate. (wikipedia.org)
Levels1
- Pulmonary exposure to WF alone increased the levels of ceramide-1-phosphate and lyso-phosphatidylinositol (18:0) which are both markers of inflammation. (cdc.gov)
Analysis2
- 2019) Analysis of the roles of phosphatidylinositol-4,5- bis phosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2. (yeastgenome.org)
- Phosphatase activity and SDS-PAGE analysis showed dose-dependent inactivation and aggregation. (aston.ac.uk)
Domains1
- One core TORC2 subunit ( Avo1 ) and two TORC2--associated regulators ( Slm1 and Slm2 ) contain pleckstrin homology (PH) domains that exhibit specificity for binding phosphatidylinositol-4,5- bis phosphate (PtdIns4,5P2). (yeastgenome.org)
Phosphorylation2
- Like other kinases, PI3K adds a cluster of oxygen and phosphorus atoms (a phosphate group) to other proteins through a process called phosphorylation. (nih.gov)
- The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. (antibodies-online.com)
Phosphoinositide1
- Lithium is a potent noncompetitive inhibitor of inositol monophosphatases, enzymes involved in phosphoinositide (PI) and inositol phosphate metabolism. (elsevierpure.com)
Biosynthetic process2
- Link to all annotated objects annotated to phosphatidylinositol phosphate biosynthetic process. (planteome.org)
- Link to all direct and indirect annotations to phosphatidylinositol phosphate biosynthetic process. (planteome.org)
Inositol5
- Phosphatidylinositols in which one or more alcohol group of the inositol has been substituted with a phosphate group. (nih.gov)
- Inositol 1-phosphate levels did not increase upon stimulation, nor was inositol 4-phosphate converted rapidly to inositol. (ox.ac.uk)
- Thus, under these conditions, at least 50% of [3H]inositol phosphates were derived from [3H]phosphatidylinositol 4,5-bisphosphate. (ox.ac.uk)
- These actions were approximately ten times more potent than the effects of these compounds on [3H]inositol phosphate accumulation, indicating that homogenous mixing of exogenous and cell-membrane-derived Ins(1,4,5)P3 does not occur. (ox.ac.uk)
- Although ibotenate-stimulated accumulation of [ 3 H]inositol 1-phosphate ([ 3 H]IP 1 ) increased significantly in the HIPP 24 h, 5 days, and 15 days after the last seizure of fully developed HIPP-kindled rats, no statistically significant increase was found in the HIPP 30 days after the last seizure. (elsevierpure.com)
Protein3
- The altered protein is less able to add ethanolamine phosphate to the end of GPI anchors. (medlineplus.gov)
- This page lists protein targets that have been identified by the IDG program that are currently understudied. (nih.gov)
- 7. Membrane targeting of protein tyrosine phosphatase PTPL1 through its FERM domain via binding to phosphatidylinositol 4,5-biphosphate. (nih.gov)
Catalytic1
Metabolism3
- Towards understanding non-canonical phosphatidylinositol kinases in the maintenance of prostate metabolism. (europa.eu)
- A strong body of evidence suggests that a large network of signaling enzymes, called lipid kinases, can add phosphate tags onto components of cellular membranes and are essential hubs for regulating cell metabolism. (europa.eu)
- 2018. Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy. . (cornell.edu)
Enzyme called1
- The PIGO gene provides instructions for making one part of an enzyme called GPI ethanolamine phosphate transfer 3 (GPI-ET3). (medlineplus.gov)
Lipid2
- The phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] acts as a signaling lipid at the plasma membrane (PM) with pleiotropic regulatory actions on multiple cellular processes. (bvsalud.org)
- MTM-6 and MTM-9 are myotubularin lipid phosphatases that function as a complex to dephosphorylate phosphatidylinositol-3-phosphate, a central regulator of endosomal trafficking. (knaw.nl)
Significantly2
- Both phosphatidylinositol-4-phosphate (PIP) and PIP 2 were increased significantly, while NCX1 current densities were decreased by 78% with no loss of NCX1 expression. (elsevierpure.com)
- The HF diet group had significantly increased abundance of triglycerides and phosphatidylinositol lipids, as well as decreased lysophosphatidic lipids and cardiolipin. (cdc.gov)
Vps341
- We demonstrate that Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate. (tamu.edu)
Kinases2
- The comet structures progressed with a constant velocity and were reminiscent of actin comets associated with motile vesicles in cells expressing type I phosphatidylinositol phosphate 5-kinases. (elsevierpure.com)
- Phosphatidylinositol 4-kinases (PI4Ks) are essential regulators of secretory and degradative transport. (nih.gov)
Membrane2
- Scholars@Duke publication: Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe. (duke.edu)
- Recent work has established membrane phospholipids such as phosphatidylinositol-4,5-bisphosphate (PIP 2 ) as potent regulators of K ATP channels controlling open probability and ATP sensitivity. (aspetjournals.org)
Search1
- View our page to search various areas of interest and methodology. (nih.gov)
Annotations1
- Comment Open annotations (there are currently 0 annotations on this page). (elifesciences.org)
Process1
- this process requires the ethanolamine phosphate at the end of the anchor. (medlineplus.gov)
Volume1
- Please specify a volume, issue and page OR a volume and page. (go.jp)
Formation1
- The chemical reactions and pathways resulting in the formation of phosphatidylinositol phosphate. (planteome.org)
Articles2
- Phosphatidylinositol phosphate may refer to: Phosphatidylinositol 3-phosphate, also known as PI(3)P Phosphatidylinositol 4-phosphate, also known as PI(4)P Phosphatidylinositol 5-phosphate, also known as PI(5)P This disambiguation page lists articles associated with the title Phosphatidylinositol phosphate. (wikipedia.org)
- Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. (aspetjournals.org)