P27kip1 protein. Medical search. Frequent questions

Inability to phosphorylate Y88 of p27(Kip1) enforces reduced p27 protein levels and accelerates leukemia progression. (mpg.de)
The G0 marker is mVenus-p27K - , a fusion protein that combines the fluorescent protein mVenus with CDK inhibitor p27 Kip1 mutant p27K - (p27 protein without CDK inhibitory function), and is usually used to identify cells in quiescent phase (G0 phase). (asn-online.org)
Moreover, pretreatment of U373 with a cSrc inhibitor (PP2), a PI3K inhibitor (LY294002), an ERK inhibitor (PD98059), or a p38 MAPK inhibitor (SB203580) but not a JNK inhibitor (SP600125) significantly reduced the magnolol-induced increases of p27/Kip1 protein levels and apoptosis. (tmu.edu.tw)
Moreover, the results of the present study supports the prognostic value of p27 Kip1 protein expression for patients diagnosed with renal cell carcinoma. (uni-luebeck.de)
Molecular mechanism of cell cycle blockage of hepatoma SK-Hep-1 cells by Epimedin C through suppression of mitogen-activated protein kinase activation and increased expression of CDK inhibitors p21(Cip1) and p27(Kip1). (jcimjournal.com)

Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (e.g., c-abl and SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein, Rb), nucleolin and others. (bdbiosciences.com)
Cao L, Faha B, Dembski M, Tsai LH, Harlow E, Dyson N. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F. (bdbiosciences.com)

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. (neobiotechnologies.com)
Cyclin-dependent kinase inhibitor p27Kip1 expression and interaction with other cell cycle-associated proteins in mammary carcinoma. (ox.ac.uk)
This leads to stabilization and nuclear localization of FOXO3A resulting in transcriptional activation of the cyclin-dependent kinase inhibitor P27KIP1. (ku.edu)
MEN 4 is caused by an inactivating mutation of the CDKN1B gene, which codes for the cyclin dependent kinase inhibitor 1B protein, also known as p27 or p27KIP1. (merckmanuals.com)

The CDKN1B gene provides instructions for making a protein called p27. (medlineplus.gov)
Most of the CDKN1B gene mutations that cause multiple endocrine neoplasia type 4 change single protein building blocks (amino acids) in the p27 protein. (medlineplus.gov)

B56Î³3 overexpression decreased phosphorylation at Thr157 (phospho-Thr157), whose phosphorylation promotes cytoplasmic localization of p27KIP1, whereas B56Î³3 knockdown significantly increased the level of phospho-Thr157. (bvsalud.org)
B56Î³3 did not increase p27KIP1 nuclear localization by down-regulating the upstream kinase Akt activity and outcompeted a myristoylated constitutively active Akt (Aktca) in regulating Thr157 phosphorylation and subcellular localization of p27KIP1. (bvsalud.org)
FoxO3a silencing resulted in decreased protein expression of FoxO3a, increased in vitro carcinogenesis and CSC characteristics, with no appreciable effects on AMPK and ACC phosphorylation, and displayed similar activities to those neutralizing the effects of casticin on in vitro carcinogenesis and CSC characteristics. (spandidos-publications.com)
Novex IEF Gels can be used to determine the pI or to detect minor changes in a protein due to deamination, phosphorylation, or glycosylation. (thermofisher.com)

p27 expression is reduced in pancreatic adenocarcinomas and decreased protein levels of p27 may play a role in the differentiation of pancreatic cancer. (biomedcentral.com)
Next, we have shown that CCN5 protein can induce expression of estrogen receptor-α (ER-α) in mammary epithelial cells. (ku.edu)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
AA and the extracellular regulated protein kinase 1/2 (ERK1/2) blocker U0126 markedly inhibited migration, elevated smooth muscle 22 α (SM22 α ) expression, repressed VSMC proliferation, elevated miR-466f-3p and miR-425-3p expression, and suppressed miR-27a-5p and miR-128-5p expression in ox-LDL-induced VSMCs. (hindawi.com)
Additionally, alongside the sensitising effect of oleic acid on the efficacy of Herceptin it was found that it increased the expression of a protein (p27Kip1), a tumour suppresser protein, which is implicated in the development of resistance to Herceptin treatment. (freerepublic.com)
Serum release of serum-starved (G 0 ) 5L rat hepatoma cells triggers transient AhR activation and P4501A1 protein expression concomitant with the G 0 /G 1 -to-S phase transition. (aspetjournals.org)
It is widely applied in protein expression profiling experiments to identify changes in protein expression levels resulting from a disease state or drug treatment. (thermofisher.com)
Therefore, in the present investigation the expression of protein within different tissue areas obtained from renal cell carcinomas, their invasion front and corresponding histologically benign renal parenchyma was determined and statistically correlated with several tumor and patient characteristics including the disease-specific long-term survival following surgical treatment. (uni-luebeck.de)
Whether the expression of p27Kip1 can be used as a prognostic marker for renal cell cancer patients still remains to be clarified. (uni-luebeck.de)
Malnutrition, both protein-energy mal- aimed to assess the use of CD95 expression nutrition (PEM) and micronutrient defi- as a marker for following up these patients ciencies, continues to be a major health during their nutritional rehabilitation and burden in developing countries. (who.int)

They are also excellent for native, nondenaturing applications using soluble proteins. (thermofisher.com)
Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)

Some mutations impair the protein's ability to interact with regulatory proteins, while others lead to the production of an unstable version of p27 that is quickly broken down. (medlineplus.gov)

We now show that during early apoptosis limited membrane permeabilization occurs in blebs and apoptotic bodies, which allows release of proteins that may affect the proximal microenvironment before the catastrophic loss of membrane integrity during secondary necrosis. (nature.com)
Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. (nature.com)
2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)

p27, cyclin D1, and retinoblastoma (Rb) protein have been demonstrated using immunohistochemistry in 189 cases of primary breast carcinoma with long-term follow-up. (ox.ac.uk)
The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. (affbiotech.cn)
p27Kip1 is a protein that inhibits CDK/Cyclin complexes. (repositoriosaludmadrid.es)
Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)
Faha B, Ewen ME, Tsai LH, Livingston DM, Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. (bdbiosciences.com)
Cyclin-CDK inhibitors (CKIs), such as p16Ink4a, p15Ink4b, p27Kip1, and p21Cip1, are involved in the negative regulation of CDK activities, thus providing a pathway through which the cell cycle is negatively regulated. (kegg.jp)

Molecular modeling and computational analyses suggests that the Sinorhizobium meliloti periplasmic regulator protein ExoR adopts a superhelical fold and is controlled by a unique mechanism of proteolysis. (cuny.edu)

B56Î³3 overexpression enhanced nuclear localization of p27KIP1, whereas knockdown of B56Î³3 decreased p27KIP1 nuclear localization . (bvsalud.org)
However, positive correlation between nuclear p27KIP1 levels and B56Î³ levels was found only in the non- tumor parts, but not in tumor parts of these tissues , implicating a dysregulation in PP2A-B56Î³3-regulated p27KIP1 nuclear localization in these tumor tissues . (bvsalud.org)
Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
Also, we found that the CCN5-induced PI3K-AKT inactivation leads to stabilization and nuclear accumulation of P27KIP1 resulting in cell cycle arrest of TNBC cells. (ku.edu)
Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, CBP and its paralogue, p300, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. (enzolifesciences.com)

This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
Based on this function, p27 is described as a tumor suppressor protein. (medlineplus.gov)
In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 tumor suppressor protein. (kegg.jp)

However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)

Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)

The B56Î³3 regulatory subunit-containing protein phosphatase 2A outcompetes Akt to regulate p27KIP1 subcellular localization by selectively dephosphorylating phospho-Thr157 of p27KIP1. (bvsalud.org)
Here, we report regulation of p27KIP1 subcellular localization by PP2A-B56Î³3. (bvsalud.org)
We found that heat shock cognate protein 70 (Hsc70) interacts with both p57 and p27 and that the subcellular localization of Hsc70 was critical to maintain HSC cell cycle kinetics. (elsevierpure.com)
Regulation can occur through modification of the p27 protein's structure, its interaction with other proteins, or its localization within the cell. (medlineplus.gov)

In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. (neobiotechnologies.com)
Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells. (mcmaster.ca)

The long-term research goal of the lab is to apply computer modeling to gain insight into cellular signal transduction pathways, specifically to provide deeper insight into both the normal and aberrant subcellular targeting of domains contained in proteins which are part of macromolecular complexes and function in various biological processes. (cuny.edu)

2DE can resolve thousands of protein "spots" on a single gel and is considered a key method in proteomics research. (thermofisher.com)
This innovative system makes sensitive protein profiling accessible to both novice and experienced proteomics researchers. (thermofisher.com)

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. (cancerindex.org)
The migration, phenotypic transformation, and proliferation of VSMCs lead to vascular wall remodeling, which is mediated by activation of extracellular regulated protein kinases 1/2 (ERK1/2) signalling [ 9 ]. (hindawi.com)

Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)

Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)

These results indicate that limited membrane permeabilization occurs in blebs and apoptotic bodies before secondary necrosis, leading to acute and localized release of immunomodulatory proteins during the early phase of active apoptotic membrane blebbing. (nature.com)

She first explored the role of the PrPSC protein in scrapie pathogenesis and also innovated new methods of gene transfer in organotypic hippocampal brain slices to explore kainate receptor biology and electrophysiology. (wikipedia.org)
Scarpati M, Heavner ME, Wiech E, Singh S. Proteomic Tools for the Analysis of Cytoskeleton Proteins.Methods Mol Biol. (cuny.edu)
Analyzing complex protein samples using traditional two-dimensional electrophoresis methods is tedious and time-consuming. (thermofisher.com)
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)

The B56Î³-containing protein phosphatase 2A (PP2A-B56Î³) has been postulated to have tumor suppressive functions. (bvsalud.org)
ATR-Chk1-mediated protein degradation of Cdc25A protein phosphatase is also a mechanism conferring intra-S-phase checkpoint activation. (kegg.jp)

This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)

For example, when p27 is held (sequestered) in the fluid that surrounds the nucleus (the cytoplasm) instead of being transported into the nucleus, the protein is unavailable to block cell cycle progression. (medlineplus.gov)

But it is not associated with cell cycle proteins. (biomedcentral.com)
We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)
The protein is involved in cellular growth and development and has an important role in regulating the cell cycle. (merckmanuals.com)
Previously, we demonstrated that magnolol, a hydroxylated biphenyl compound isolated from the bark of Magnolia officinalis, at low concentrations (3-10 μM) exerted an antiproliferation effect in colon cancer, hepatoma, and glioblastoma (U373) cell lines through upregulation of the p21/Cip1 protein. (tmu.edu.tw)
studies suggest that certain endocrine cells may be particularly dependent on the p27 protein to control cell division. (medlineplus.gov)

Epigenetic regulation refers to the multiple chemical modifications of DNA or DNA-associated proteins that alter chromatin structure and DNA accessibility in a heritable manner, without changing DNA sequence. (frontiersin.org)
To date, most of our understanding of epigenetic regulation and its participation in leukemogenesis is based on the enzymatic activities and protein-protein interactions of histone and DNA modifiers. (frontiersin.org)
Can be used to study CBP kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example [ 3 H]-Acetyl CoA. (enzolifesciences.com)
However, changes in regulation that reduce the amount or function of the p27 protein in the nucleus are found in many types of cancer. (medlineplus.gov)

Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
RÉSUMÉ La malnutrition continue de faire peser un lourd fardeau sanitaire sur les pays en développement. (who.int)
Malnutrition, both protein-energy mal-nutrition (PEM) and micronutrient deficiencies, continues to be a major health burden in developing countries. (who.int)

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. (rndsystems.com)

High performance NuPage Bis-Tris gels provide a neutral pH environment for the second dimension that reduce in-gel protein degradation. (thermofisher.com)

During conventional IEF, amphoteric molecules, like proteins, migrate until they reach a region where the pH of the matrix matches their isoelectric point and they "focus" into sharp bands. (thermofisher.com)
Salts, ionic molecules and ionic detergents interfere with isoelectric focusing and compromise protein separation. (thermofisher.com)

500) are usually designated as tannins, which refers to their ability to interact with proteins. (indexindex.com)

We hypothesized that single-nucleotide polymorphisms that impaired p27Kip1 function could render patients refractory to endocrine therapy but responsive to CDK4/6 inhibitors, narrowing the patient subpopulation that requires CDK4/6 inhibitors. (repositoriosaludmadrid.es)
however, palbociclib blocks CDK4 kinase activity regardless of the p27Kip1 status. (repositoriosaludmadrid.es)
CDK4 is a member of the Ser/Thr protein kinase family. (thermofisher.com)

She found that p27 regulates the differentiation of oligodendrocytes by modulating transcription of the gene for myelin basic protein. (wikipedia.org)
We found that the p27Kip1 V109G single-nucleotide polymorphism is homozygous in approximately 15% of hormone-positive breast cancer patients. (repositoriosaludmadrid.es)
This protein is found in cells and tissues throughout the body. (medlineplus.gov)

However, a growing body of evidence suggests that recruitment, stability, and function of epigenetic factors can be mediated by non-protein-coding RNAs. (frontiersin.org)

What does this gene/protein do? (cancerindex.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)

Based on recent data, a new view is emerging that vertebrate Dachshund(Dach) proteins are components of Six1/6 transcription factor-dependent signaling cascades. (biologists.com)

Furthermore, we demonstrated that p27KIP1 levels are positively correlated with B56Î³ levels in both non- tumor and tumor parts of a set of human colon tissue specimens. (bvsalud.org)
Avian species do not have brown adipose tissue (BAT), and lack Uncoupling Protein 1 ( UCP1 ) [ 12 ]. (biomedcentral.com)

Captured proteins are visualized using chemiluminescent detection reagents (Step 3) . (rndsystems.com)

Figure 2: Postulated G-protein-mediated signal transduction process in normal and regenerating endothelial cells. (nature.com)

The most enriched proteins released were the nucleosomal histones, which have previously been identified as damage-associated molecular pattern proteins (DAMPs) that can initiate sterile inflammatory responses. (nature.com)
The second dimension consists of a conventional SDS-PAGE electrophoretic separation where proteins are differentiated based on their molecular weights. (thermofisher.com)

Glutamate excitation induced a rapid alteration in the AMP:ATP ratio that was associated with the activation of the AMP-activated protein kinase (AMPK). (jneurosci.org)

Arguelles J, Lee J, Cardenas LV, Govind S, Singh S. In Silico Analysis of a Drosophila Parasitoid Venom Peptide Reveals Prevalence of the Cation-Polar-Cation Clip Motif in Knottin Proteins. (cuny.edu)
Sample preparation is a fundamental step in any protein analysis workflow, but it is even more crucial in 2DE electrophoresis where no universal method exists. (thermofisher.com)

In addition to functions which overlap with those of p300, CBP has a number of unique functions, including a role in p27Kip1 induction. (enzolifesciences.com)

It acts as a tumor suppressor and when the protein is lost, cells can undergo unscheduled replication, which may eventually lead to cancer. (merckmanuals.com)

Anatomy30

Organisms11

Diseases20

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes57

Disciplines and Occupations1

Information Science3

Health Care1

KIP15

Retinoblastoma Protein2

Inhibitor4

CDKN1B2

Phosphorylation4

Expression10

Soluble proteins2

Regulatory proteins1

Apoptosis3

Cyclin7

Regulator1

Nuclear5

Tumor suppressor protein3

Signalling pathway1

Antibodies1

Localization4

Antibody2

Subcellular1

Proteomics2

Kinases2

Cyclins1

Transcriptional1

Membrane1

Methods4

Phosphatase2

Catalytic1

Progression1

Cell6

Regulation4

Malnutrition3

Acute1

Degradation1

Molecules2

Interact1

CDK43

Found3

RNAs1

Gene protein2

Transcription1

Tissue2

Reagents1

Endothelial cells1

Molecular2

Activation1

Analysis2

Functions1

Cells1