P21 cip1 protein show that p21. Medical search. Frequent questions

This gene encodes the cyclin-dependent kinase inhibitor p21(WAF1/Cip1), a factor implicated in cell cycle, senescence, and cancer. (ed.ac.uk)
This new and unprecedented evidence for a link between SdhD dysfunction and p21(WAF1/Cip1) will open new avenues for the study of the mechanisms that cause tumors in Sdh mutants. (ed.ac.uk)
Transcriptional activation of p21(waf1/cip1) by alkylphospholipids: role of the mitogen-activated protein kinase pathway in the transactivation of the human p21(waf1/cip1) promoter by Sp1. (conicet.gov.ar)
We have shown previously that perifosine induces p21(waf1/cip1) in a p53-independent fashion and that induction of p21(waf1/cip1) is required for the perifosine-induced cell cycle arrest because cell lines lacking p21(waf1/cip1) are refractory to perifosine. (conicet.gov.ar)
In this report, we investigated the mechanism by which perifosine induces p21(waf1/cip1) protein expression. (conicet.gov.ar)
We observed that perifosine induces the accumulation of p21(waf1/cip1) mRNA without affecting p21(waf1/cip1) mRNA stability. (conicet.gov.ar)
Using several p21(waf1/cip1) promoter-driven luciferase reporter plasmids, we observed that perifosine activates the 2.4-kb full-length p21(waf1/cip1) promoter as well as a p21 promoter construct lacking p53-binding sites, suggesting that perifosine activates the p21(waf1/cip1) promoter independent of p53. (conicet.gov.ar)
Mutations in each particular Sp1 site block perifosine-induced p21(waf1/cip1) expression. (conicet.gov.ar)
Moreover, we showed that perifosine activates the mitogen-activated protein/extracellular signal-regulated kinase pathway, and this activation promotes the phosphorylation of Sp1 in known mitogen-activated protein kinase residues (threonine 453 and 739), thereby leading to increased Sp1 binding and enhanced p21(waf1/cip1) transcription. (conicet.gov.ar)
Protein levels of CDKN1A -encoded p21 CIP1/WAF1 were determined by western blotting and the importance of p53 was shown by immunofluorescence and by a loss-of-function approach. (biomedcentral.com)
Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
AIM: The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. (bvsalud.org)
CONCLUSION: p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. (bvsalud.org)
Subcellular localisation of cyclin B, Cdc2 and p21(WAF1/CIP1) in breast cancer. (ox.ac.uk)
The heterodimeric cyclin B/Cdc2 protein kinase governs entry into mitosis, and can be negatively regulated through p53-mediated transcriptional induction of the cyclin-dependent kinase inhibitor p21(WAF1/CIP1). (ox.ac.uk)
Ectopic expression of p21(WAF1/CIP1) in cultured cells has been shown previously to influence the subcellular distribution of the cyclin-dependent kinases (CDKs) including Cdc2. (ox.ac.uk)
In this study, we have examined the subcellular localisation of Cdc2, cyclin B and p21(WAF1/CIP1) by immunohistochemistry in a well characterised series of primary breast cancers. (ox.ac.uk)
cytoplasmic p21(WAF1/CIP1) and high cyclin B levels were also significant predictors of poor prognosis. (ox.ac.uk)
Investigation of the subcellular distribution of cell cycle regulatory proteins, particularly p21(WAF1/CIP1), could provide valuable prognostic markers in breast cancer. (ox.ac.uk)
In addition, BMP-2 overexpression induced p21 WAF1/CIP1 and p27 KIP1 expression, and cellular apoptosis in RCC cells. (oncotarget.com)
AKT prevents cell death by inactivating proapoptotic factors including BAD, procaspase-9 and Forkhead transcription factor family proteins (FOXOs), activates transcription factors that upregulate antiapoptotic genes, including NF-kB, inactivates p53 through Mdm2, and phosphorylates the cell cycle inhibitors p21 CIP1/WAF1 and p27 KIP1 , thus increasing cell proliferation (Hennessy et al. (ebrary.net)
Western blot analysis was done using anti pRB, anti-p21^(cip1/waf1), anti-p53, anti-p27^(kip1), anti-cyclinE, anti-CDK2 antibodies to detect the presence and expression of these proteins in PCA treated leiomyoma cells. (dsmc.or.kr)
From Western blot analysis, it revealed PCA induced the expression of p21^(cip1/waf1) and p27^(kip1) increasingly and was not mediated by p53. (dsmc.or.kr)
In conclusion, PCA, a polyphenol antioxidant, inhibited cell proliferation and induced cell cycle arrest at sub G1 phase by enhancing the production of p21^(cip1/waf1) and p27^(kip1). (dsmc.or.kr)
이러한 기전에 대하여 세포주기 관련 유전자 발현도를 측정한 결과 p21^(cip1/waf1)과 p27^(kip1)은 PCA의 처리 농도에 비례하여 발현도가 증가하였으며, cyclin E, A, D1 및 Bcl-2는 농도에 비례하여 발현의 감소를 확인하였다. (dsmc.or.kr)
결론적으로 PCA에 의해서 자궁근종세포의 증식억제가 일어나며 이러한 현상은 세포자멸사에 의한 것이며 그 기전은 p21^(cip1/waf1)과 p27^(kip1)의 발현 증가 및 pRB 인산화와 Bcl-2의 감소 로 인한 세포자멸사를 확인하였다. (dsmc.or.kr)
Purpose: The study investigated whether basal, constitutive levels of p21(WAF1/CIP1) protein in murine carcinoma are related to in vivo tumor radioresponse. (elsevierpure.com)
The study is based on recent observations demonstrating that in vitro cancer cell lines are resistant to cytotoxic drugs when they express high basal levels of p21(WAF1/CIP1) protein, and that the loss of the p21 gene in the HCT116 human colorectal cancer cell line results in increased radioresponse of xenografts derived from that cell line. (elsevierpure.com)
Methods and Materials: Protein levels of p21(WAF1/CIP1), p53, bax, and bcl-2 were determined in 8 carcinomas (3 mammary carcinomas designated MCa-4, MCa-29, and MCa-35, 2 squamous cell carcinomas designated SCC-IV and SCC-VII, ovarian adenocarcinoma OCa-I, hepatocarcinoma HCa-I, and adenosquamous carcinoma ACa SG) syngeneic to C3Hf/Kam mice using Western blot analysis. (elsevierpure.com)
Results: Protein levels of these oncogenes varied among tumors, with p21(WAF1/CIP1) showing the greates variation: its mean densitometric value ranged from 1 to 19. (elsevierpure.com)
Tumor radioresponse correlated significantly (R = 0.77, p = 0.02) only with the magnitude of p21(WAF1/CIP1) expression: tumors with high levels of p21(WAF1/CIP1) were less radiocurable than those with lower levels. (elsevierpure.com)
Despite a strong trend to correlation, (p = 0.15), p21(WAF1/CIP1) expression did not correlate significantly with radiation-induced apoptosis, which suggested that p21(WAF1/CIP1) influenced tumor radioresponse by mechanisms beyond that of apoptosis induction. (elsevierpure.com)
Conclusion: Our findings showed that murine tumors exhibit wide variation in constitutive levels of p21(WAF1/CIP1) which had a significant relationship with tumor radioresponse: tumors with high levels of p21(WAF1/CIP1) were less radiocurable than those with lower levels. (elsevierpure.com)
These findings support the concept that p21(WAF1/CIP1) is a major determinant of tumor radioresponse in vivo, and may have important clinical implications. (elsevierpure.com)
The pretreatment assessment of p21(WAF1/CIP1) protein could serve as a useful predictor of radiotherapy outcome and may assist in selecting an effective treatment modality. (elsevierpure.com)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
EBF3 bound directly to p21 cip1/waf1 promoter and regulated transcription from both p21 cip1/waf1 and p27 kip1 promoters in reporter assays. (aacrjournals.org)
JQ1 treatment resulted in significant changes in expression of genes that play important roles in glioblastoma such as c-Myc, p21(CIP1/WAF1), hTERT, Bcl-2, and Bcl-xL. (ox.ac.uk)
In contrast, ectopically expressed Bcl-xL partially rescued cells from JQ1-induced apoptosis, and knockdown of p21(CIP1/WAF1) attenuated JQ1-induced cell-cycle arrest. (ox.ac.uk)
encoding p21(waf1/cip1) and gene beneath the control of a p53-reactive promoter. (cyberdakwah.net)

Western blot analysis of normal skin and tumor lysates showed that silibinin decreases the levels of cyclin-dependent kinase 2 and cyclin-dependent kinase 4 and associated cyclins A, E, and D1, together with an up-regulation of Cip1/p21, Kip1/p27, and p53. (nih.gov)
The CDK4/Cyclin D complex is also negatively regulated by p21/CIP1/CDKN1A and p27/Kip1. (rndsystems.com)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
Cell cycle progression is faster in cells in which invadopodia are abolished (by Tks5 knockdown), evidenced by earlier induction of cyclins A and B. A close look at the regulators of G1 revealed that the overexpression of p27 kip1 , but not p21 cip1 , causes faster turnover of invadopodia and increased ECM degradation. (biorxiv.org)
In recent years, cell cycle regulators have been shown to exhibit roles in both tumor suppression and tumor promotion, particularly cyclin-dependent kinase inhibitors (CKI) p27 kip1 and p21 cip1 14 . (biorxiv.org)
Our data show that knock-down of p27/Kip1 expression significantly suppressed the magnolol-induced apoptosis, suggesting that p27/Kip1 might play an important role in the regulation of magnolol-induced apoptosis. (tmu.edu.tw)
Moreover, pretreatment of U373 with a cSrc inhibitor (PP2), a PI3K inhibitor (LY294002), an ERK inhibitor (PD98059), or a p38 MAPK inhibitor (SB203580) but not a JNK inhibitor (SP600125) significantly reduced the magnolol-induced increases of p27/Kip1 protein levels and apoptosis. (tmu.edu.tw)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)

Together, these results show a strong preventive efficacy of silibinin against photocarcinogenesis, which involves the inhibition of DNA synthesis, cell proliferation, and cell cycle progression and an induction of apoptosis. (nih.gov)
Gain of function and loss of function experiments showed that PDCD4 induced turnover (proliferation and apoptosis) of HUVECs. (plos.org)
Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis. (bvsalud.org)
Enhanced apoptosis was correlated with loss of pro-survival factors (XIAP, bcl-2, bcl-xL), expression of pro-apoptotic markers (caspases 3/7, PARP cleavage) and enhanced cell cycle regulators p21 and p27. (biomedcentral.com)
Recently, Rb has been shown to play a role in apoptosis. (novusbio.com)
We have previously shown the capacity of one PAPU compound, (E)-1-(3,4-dihydroxyphenethyl)-3-styrylurea (PAPU1), to induce caspase-dependent apoptosis in mela-noma cells. (molcells.org)
Tumor radiocurability showed a significant positive correlation (p = 0.02) with the extent of radiation induced apoptosis, indicating that tumors that responded to radiation with higher percentages of apoptosis were more curable by radiation. (elsevierpure.com)
We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
RESULTS: We showed that JQ1 induced marked G1 cell-cycle arrest and apoptosis, which was phenocopied by knockdown of individual BET family members. (ox.ac.uk)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)

This was related to the inhibition of cell cycle regulatory factors, including cyclin D/E and cyclin-dependent kinase (CDK) 2/4, through induction of p21 Cip1 . (molcells.org)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)

The cyclin-dependent kinase (CDK) inhibitors p21 and p16 inhibit the activity of CDKs, such as CDK4. (medscape.com)

Here, we show that p16 Ink4a deficiency enhances fasting-induced hepatic glucose production in vivo by increasing the expression of key gluconeogenic genes. (diabetesjournals.org)
SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)

Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
Specifically, the PDCD4 protein combines directly with the mRNA coding region of the target gene ( MYB/c-MYB ) to block translation [2] . (plos.org)
Consistent with this, mRNA and protein levels of the invadopodia key components, cortactin and MT1-MMP, peak at 14 hours post-release. (biorxiv.org)
Yet, this description is normally insufficient to comprehend why TSA markedly decreases P21 and PIG3 mRNA amounts in the current presence of Nutlin-3. (cyberdakwah.net)
Nevertheless, although this little reduction in p53 synthesis by TSA may be the root trigger, it isn't enough to comprehend our observations on PIG3 and P21 mRNA amounts, that the unwanted effects of TSA in Nutlin-3 cotreated cells had been a lot more pronounced. (cyberdakwah.net)
In a far more Rabbit Polyclonal to PAK5/6 extreme circumstance, that of HNDFs, HDM2 mRNA amounts did not lower in any way, whereas P21 and PIG3 mRNAs had been reduced significantly (Amount 3). (cyberdakwah.net)

ST also caused the depolarization of mitochondrial membrane potential and increased Bax/Bcl-2 protein ratio. (biomedcentral.com)
Col-lectively, our results suggest that G 1 cell cycle regulatory proteins and mitochondrial pathways are the crucial targets of PAPU1 in the chemoprevention of breast cancer cells. (molcells.org)
The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. (nih.gov)

In addition, Runx2 has been shown to interact with several kinases that contribute to facilitate cell-cycle dependent dynamics via direct protein phosphorylation. (wikipedia.org)
Silibinin also showed a strong phosphorylation of extracellular signal-regulated protein kinase 1/2, stress-activated protein kinase/c-JUN NH2-terminal kinase 1/2, and p38 mitogen-activated protein kinases but inhibited Akt phosphorylation and decreased survivin levels with an increase in cleaved caspase-3. (nih.gov)
Each stage of the cell cycle is profiled by distinct protein complexes and phosphorylation events. (janechin.net)

Furthermore, Runx2 controls the gene expression of cyclin D2, D3, and the CDK inhibitor p21(cip1) in hematopoietic cells. (wikipedia.org)
It has been shown that on a molecular level, Runx associates with the cdc2 partner cyclin B1 during mitosis. (wikipedia.org)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

The minimal p21 promoter region required for perifosine-induced p21 promoter activation contains four consensus Sp1-binding sites. (conicet.gov.ar)
Another explanation could be that TSA's raising influence on (p21) transcription is normally associated with its capability to decrease c-myc amounts,27, 28 as c-myc can decrease p21 amounts.29, 30, 31, 32 Because p53, like TSA, represses the promoter,33, 34 further inhibition of c-myc expression by TSA could haven't any consequence. (cyberdakwah.net)
To comprehend why the (p21) promoter will be even more sensitive to a little reductions in p53 compared to the promoter in nutlin-3-treated cells, we propose a LY2794193 model predicated on the two pursuing reports: First, it's been proven that and promoters are destined by p53 and hdm2 which the binding of hdm2 is certainly associated with a decrease in the promoters' activity. (cyberdakwah.net)

The two SDHD-ESR cell lines also showed accumulation of this protein. (ed.ac.uk)

Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)

Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)

Runx2 is responsible for inducing the differentiation of multipotent mesenchymal cells into immature osteoblasts, as well as activating expression of several key downstream proteins that maintain osteoblast differentiation and bone matrix genes. (wikipedia.org)
Otros autores sugieren que ese es el resultado de mutaciones en oncogenes, genes supresores y enzimas de la vía glucolítica o del metabolismo oxidativo mitocondrial (Myc, Akt, p53, HIF1-α). (researchgate.net)
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. (lookformedical.com)
The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS. (lookformedical.com)
This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. (nih.gov)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)

Data table showing topics related to specific cancers and associated disorders. (cancerindex.org)
These proteins recently emerged as important therapeutic targets in NUT midline carcinoma and several types of hematopoietic cancers. (ox.ac.uk)

Previously, we demonstrated that magnolol, a hydroxylated biphenyl compound isolated from the bark of Magnolia officinalis, at low concentrations (3-10 μM) exerted an antiproliferation effect in colon cancer, hepatoma, and glioblastoma (U373) cell lines through upregulation of the p21/Cip1 protein. (tmu.edu.tw)

Subsequently, mTOR phosphorylates key eukaryotic translation regulators, including p70-S6 kinase (p70S6K) and the eukaryotic translation initiation factor 4E binding protein 1 (4E-BP1) (Hay and Sonenberg 2004). (ebrary.net)
In the present review, the role of Jak/STAT proteins and their regulators will be discussed in connection with osteoclastogenesis, since growing evidence indicates that a number of cytokines and growth factors utilizing Jak/STAT signaling pathways affect osteoclastogenesis. (koreascience.kr)

Resultados: En este contexto, la huella bioenergética también ha resultado ser un marcador de la respuesta a quimioterapia en distintas células tumorales en cultivo, en tumores humanos y en modelos murinos como en pacientes con carcinoma de colon. (researchgate.net)
In this study, using invadopodia- and cell cycle- fluorescent markers, we show in 2D and 3D cell cultures, as well as in vivo , that breast carcinoma cells assemble invadopodia and invade into the surrounding ECM preferentially during the G1 phase of the cell cycle. (biorxiv.org)
We investigated whether impaired regulation of bone morphogenetic protein-2 (BMP-2) via epigenetic pathways is associated with renal cell carcinoma (RCC) pathogenesis. (oncotarget.com)

This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

Flow cytometric analysis showed that PAPU1 blocked cell cycle progression in the G 0 /G 1 phase, and reduced the proportion of cells in G 2 /M phase. (molcells.org)

New regulatory targets were discovered and novel functions of known proteins were uncovered. (janechin.net)

Runx2 protein is detected in preosteoblasts and the expression is upregulated in immature osteoblasts and downregulated in mature osteoblasts. (wikipedia.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Our recent report indicated that tumor suppressor gene ( p53 ) mutations and protein aberrant expression were detected in pterygium. (molvis.org)
To investigate the involvement of HPV 16/18 E6 in p53 inactivation in pterygium, the association between HPV 16 or HPV 18 infection, the HPV E6 oncoprotein, and p53 protein expression was analyzed in this study. (molvis.org)
In addition, p53 protein negative expression in pterygium was correlated with HPV16/18 E6 oncoprotein expression but not with a p53 mutation. (molvis.org)
In this report, we analyzed p53 protein expression and gene mutation in pterygium in comparison with HPV 16/18 infection and E6 oncoprotein expression in pterygium tissues to understand whether the HPV infection was involved in pterygium pathogenesis. (molvis.org)
Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL). (lookformedical.com)
Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
Intracellular zinc homeostasis regulated by a complex of EVER proteins and zinc transporter proteins may play a role in inhibiting EV-HPV expression. (medscape.com)

What pathways are this gene/protein implicaed in? (cancerindex.org)
The eukaryotic DNA polymerase sliding clamp, proliferating cell nuclear antigen or PCNA, is a ring-shaped protein complex that surrounds DNA to act as a sliding platform for increasing processivity of cellular replicases and for coordinating various cellular pathways with DNA replication. (rcsb.org)
The ULK1 protein complex including ULK1, ATG13, and FIP200 coordinates the autophagy initiation from different upstream signaling pathways to induce autophagy [ 6 , 7 ]. (hindawi.com)
While AKT phosphorylates multiple downstream targets (see above), the emerging picture is that the ability of AKT to coordinate mitogenic and nutrient-sensing pathways controlling protein synthesis is a key mechanism by which AKT regulates cell proliferation. (ebrary.net)

The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

Interestingly, recent data suggest a function for ULK1 not only during autophagy activation but also during elongation and closure of the autophagosomal membrane via binding to ATG8 proteins [ 8 ]. (hindawi.com)
However, recent studies have suggested that BRAFi/MEKi and ERK1/2i resistance can arise through activation of a parallel signalling pathway leading to activation of ERK5, an unusual protein kinase that contains both a kinase domain and a transcriptional transactivation domain. (babraham.ac.uk)
They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)

Mutations in the gene for the antennapedia homeodomain protein are associated with the conversion of antenna to leg or leg to antenna DROSOPHILA. (lookformedical.com)

Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. (wikipedia.org)
In OA, while it has been shown to reduce the severity of the disease in the murine model [ 8 , 16 ], the senolytic UBX0101 failed to demonstrate clinical efficacy in humans. (aging-us.com)

Here, we investigate amplification events that underlie resistance to the MEK inhibitor selumetinib (AZD6244/ARRY-142886) in COLO205 cells, a well-characterized model for reproducible emergence of drug resistance, and show that amplifications acquired are the primary cause of resistance. (babraham.ac.uk)
En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
Other reports also show that DN is highly associated with accelerated aging in various types of cells such as tubular cells, podocytes, mesangial cells, and endothelial cells [ 21 - 23 ]. (hindawi.com)
ST (30-90 μM) treatments for 48 h and 72 h did not show any significant effect on cell growth and death in A431 cells. (biomedcentral.com)
This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)
Matrix assisted laser desorption ionization (MALDI) mass spectrometry is commonly used to analyze biological molecules such as proteins, peptides and lipids from cells or tissue. (koreascience.kr)
The results of FACS analysis showed that there was increased the percentage of cells in sub G1. (dsmc.or.kr)
Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. (thermofisher.com)

This gene encodes the GA-binding protein transcription factor, beta subunit. (nih.gov)

For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
This locus, however, also encodes a protein from an alternative reading frame, designated p19ARF. (medscape.com)

Autophagosomes engulf cytoplasmatic components and deliver these cargos, for example, long-lived proteins or damaged mitochondria, to lysosomes for degradation. (hindawi.com)

We show that the mutation markedly alters the binding energetics for some client proteins, while another, p21(CIP1), is only mildly affected. (rcsb.org)

Structures of the disease variant bound to peptides derived from two PCNA partner proteins reveal that the binding pocket can adjust conformation to accommodate some ligands, indicating that the binding site is dynamic and pliable. (rcsb.org)

Here we show that eight different ERKi (both catERKi or dmERKi) drive the turnover of ERK2, the most abundant ERK isoform, with little or no effect on ERK1. (babraham.ac.uk)
Thermal stability assays show that ERKi do not destabilise ERK2 (or ERK1) in vitro, suggesting that ERK2 turnover is a cellular consequence of ERKi binding. (babraham.ac.uk)

TSA inhibits the result of Nutlin-3 on p21 The reported reduced amount of c-myc amounts by TSA27 previously, 28 may describe why in the current presence of TSA also, Nutlin-3 does not additional boost p21 amounts. (cyberdakwah.net)

This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. (cancerindex.org)

The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. (wikipedia.org)
Proteins which bind to DNA. (lookformedical.com)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. (thermofisher.com)
The bromodomain and extraterminal domain (BET) proteins are epigenetic readers that selectively bind to acetylated lysine residues on histone tails. (ox.ac.uk)
ZnT-1/EVER has been found to bind the E5 protein, resulting in increased concentrations of free zinc in keratinocytes. (medscape.com)

Invadopodia are cancer cell protrusions rich in structural proteins (e.g. (biorxiv.org)

HPV 16/18 infection was detected by nested-polymerase chain reaction (nested-PCR), the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas. (molvis.org)

EXPERIMENTAL DESIGN: The antineoplastic effects of JQ1 were shown using ex vivo cultures derived from primary glioblastoma xenograft lines and surgical specimens of different genetic background. (ox.ac.uk)

Transcript variants of the gene that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. (wikipedia.org)

Several other genetic backgrounds result in enlargement of the haltere significantly beyond the normal range of haploinsufficient phenotypes, suggesting genetic variation in cofactors that mediate homeotic protein function. (lookformedical.com)

The mutation lies near the binding site for most PCNA-interacting proteins. (rcsb.org)

Immunoblot analysis was carried out to investigate changes in levels of cleaved PARP, XIAP, bcl-2, bcl-xL, p21 and p27. (biomedcentral.com)
Bcl-2 levels also showed broad variation in densitometric values, from 1 to 10. (elsevierpure.com)

Autophagy functions in tumor suppression by, for example, preserving protein and organelle homeostasis. (hindawi.com)

It is of fundamental importance in determining PROTEIN CONFORMATION. (lookformedical.com)

Anatomy18

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WAF140

Kip18

Apoptosis12

Induction2

Inhibitors p211

Ink4a2

MRNA6

Mitochondrial3

Phosphorylation3

Cyclin3

Promoter3

Accumulation1

Cytoplasmic1

Induces1

Genes6

Cancers2

Upregulation1

Regulators2

Carcinoma3

CDK42

Cell cycle progression1

Regulatory1

Expression9

Pathways4

Mdm21

Activation4

Mutations1

Humans2

Cells9

Gene encodes1

Encodes2

Mitochondria1

Markedly1

Peptides1

Turnover2

Inhibits1

Progression1

Bind6

Structural1

Immunohistochemistry1

Glioblastoma1

Promoters1

Significantly1

Mutation1

Levels2

Tumor suppression1

Conformation1