P16ink4a protein expression. Medical search. Frequent questions

Here, we show that p16 Ink4a deficiency enhances fasting-induced hepatic glucose production in vivo by increasing the expression of key gluconeogenic genes. (diabetesjournals.org)
In this model, the senescence-sensitive promoter from the cyclin-dependent kinase inhibitor 2A ( Cdkn2a ) gene, also known as p16 INK4a , drives expression of 3MR, a fusion protein that is composed of luciferase and red fluorescent protein (RFP) reporters and herpes simplex virus-1 thymidine kinase, which converts ganciclovir (GCV) into an apoptosis inducer. (jax.org)
IMR-90 fibroblast populations cultured in magnesium-deficient conditions had increased senescence-associated beta-galactosidase activity and increased p16(INK4a) and p21(WAF1) protein expression compared with cultures from standard media conditions. (wellnessresources.com)
This loss is accompanied with an increased expression of the histone demethylase Jmjd3 and with the recruitment of the MLL1 protein, and correlates with the expression of the Ink4a/Arf genes. (plos.org)
Finally, we demonstrate that Polycomb proteins and associated epigenetic marks are crucial for the control of the replication timing of the INK4a/ARF locus during senescence. (plos.org)
Conditional deletion of the Bmi-1 polycomb protein depleted pre-GEPCOT and GEPCOT cells, though pre-GEPCOT cells were more dependent upon Bmi-1 for Cdkn2a ( p16 Ink4a ) repression. (elifesciences.org)
SnCs exhibit irreversible growth arrest accompanied by increased expression of cyclin-dependent kinase inhibitors (CDKi) such as p16 INK4a , and p21 Cip1 , accumulation of DNA damages, and secretion of diverse bioactive molecules known as the senescence-associated secretory phenotype (SASP). (aging-us.com)
A series of 76 cases of gastrointestinal tract endocrine tumors, including 21 poorly differentiated endocrine carcinomas (PDECs, small cell carcinomas) and 55 well-differentiated endocrine neoplasms (WDENs, carcinoids), 18 metastatic and 37 nonmetastatic rectal carcinoids, were examined by immunohistochemical analysis for p16 INK4a , cyclin D1, and retinoblastoma protein (pRB) expression. (nycu.edu.tw)
The p16 INK4a /cyclin D1/pRB pathway was altered in gastrointestinal tract endocrine tumors, and the loss of expression of pRB may be helpful in identifying patients at high risk of metastasis in rectal WDENs. (nycu.edu.tw)
Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
Role of P16(INK4a) expression in identifying CIN2 or more severe lesions among HPV-positive patients referred for colposcopy after abnormal cytology. (journal-imab-bg.org)
D-cyclin-cdk activity is required for Rb phosphorylation in v-Jun-transformed cells, since ectopic expression of the cdk4- and cdk6-specific inhibitor p16(INK4A) inhibits both DNA synthesis and cell proliferation. (ox.ac.uk)
In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
Down regulation of EZH2 causes release of the ~35 kb repressive chromatin loop and induction of both INK4a and INK4b , whereas ARF expression remains unaltered. (biomedcentral.com)
Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
p15 INK4b and p16 INK4a are closely related proteins and both act on the Rb-pathway through the inhibition of the proliferation-promoting cyclin-dependent kinases CDK4 and CDK6. (biomedcentral.com)
INK4b-ARF-INK4a expression is controlled by various signal transduction pathways and patterns of expression vary depending on physiological circumstances. (biomedcentral.com)
A) Organization of the human INK4b-ARF-INK4a locus (not drawn to scale), encoding three distinct proteins, p15 INK4b , p14 ARF and p16 INK4a . (biomedcentral.com)
RT-qPCR analysis of INK4b-ARF-INK4a expression in neonatal (yellow bar) versus adult (blue bar) HDFs. (biomedcentral.com)

Smitha T, Mohan CV, Hemavathy S. Prevalence of human papillomavirus16 DNA and p16INK4a protein in oral squamous cell carcinoma: A systematic review and meta-analysis. (exp-oncology.com.ua)
Expression profiles of CDKN2A, MDM2, E2F2 and LTF genes in oral squamous cell carcinoma. (exp-oncology.com.ua)
Nevertheless, in our prior study to research the roles performed by HIV-1 Tat and risky HPV E6/E7 protein to advertise carcinogenesis in cervical malignancies, expressions of p16INK4A was discovered to be low in HIV-related squamous cell carcinoma from the uterine cervix, a relationship which has been backed by other research [23,24]. (antiviralbiologic.com)
Entirely, expressions of p16INKA4, EGFR and its own phosphorylated type pEGFR have already been suggested to reflect infections with risky HPV in squamous cell carcinoma of conjunctiva. (antiviralbiologic.com)
Purpose: We evaluated X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1) protein in head and neck squamous cell carcinoma (HNSCC) patients in association with outcome. (psu.edu)

Two powerful tumour suppressor pathways, controlled by the p53 and retinoblastoma (pRB) proteins, are important for establishing and maintaining the senescence growth arrest. (nature.com)
p16INK4a (p16) traps the cell at the restriction point of the cell cycle by binding to cyclin-dependent kinase 4/6 thus preventing the phosphorylation of the retinoblastoma protein (pRB). (qmul.ac.uk)
Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) interacts with CDK2AP2, modulates the actions of transforming growth factor-B1, cyclin-dependent kinase 2 and retinoblastoma protein, and closely interacts with micro-RNA21 and micro-RNA25. (cancerindex.org)
9. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Immunohistochemical overexpression of p16 protein associated with intact retinoblastoma protein expression in cervical cancer and cervical intraepithelial neoplasia. (journal-imab-bg.org)
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity. (ox.ac.uk)

Immunohistochemical analyses of lung tumors showed focal areas which lacked p16INK4a staining. (jax.org)
Furthermore, they demonstrate the importance of immunohistochemical studies to examine expression in tissues that contain multiple cell types, such as the lung, and in tumors that by nature are heterogeneous. (jax.org)
CONCLUSIONS: Negative p16INK4a and Ki67 immunohistochemical staining can assure absence of a higher-grade lesion in more than 85% of patients with CIN2 biopsies and can be used to prevent overtreatment of these patients. (bvsalud.org)
To assess the cases for HPV, the specimens were examined for p16 protein using an immunohistochemical (IHC) study, and then, the Chromatin In Situ Hybridization (CISH) test was performed for all positive cases. (ac.ir)
Experimental Design: XRCC1 protein expression was assessed by immunohistochemical (IHC) staining of pretreatment tissue samples in 138 consecutive HNSCC patients treated with surgery (n = 31), radiation (15), surgery and radiation (23), surgery and adjuvant chemoradiation (17), primary chemoradiation (51), and palliative measures (1). (psu.edu)
Karcheva M, Popovska S, Nachev R. Immunohistochemical investigations of P16 INK 4a expression in carcinomas and high grade cervical lesions. (journal-imab-bg.org)
7. Negri G1, Egarter-Vigl E, Kasal A, Romano F, Haitel A, Mian C. p16INK4a is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors: an immunohistochemical study with immunocytochemical correlations. (journal-imab-bg.org)

The expression of cyclin D1, cdk4, p16INK4a, and Rb protein was examined by immunohistochemistry in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced mouse lung tumors. (jax.org)

Knockdown of induces premature senescence As a crucial cell-cycle inhibitor p21Cip1 has been shown to be induced in senescent cells (Stein can cause premature senescence (McConnell expression Nesbuvir can extend the lifespan of human fibroblasts (Brown induces premature senescence. (cancerrealitycheck.com)
Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders[J]. Nature 2011 479(7372): 232-236. (paperonce.org)

Several hallmarks of cellular senescence, such as cell cycle arrest, expression of cyclin-dependent kinase inhibitors, DNA damages, and senescence-associated secretory profile were evaluated. (aging-us.com)
They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
We hypothesized that BMP4 might exert beneficial effects through modulation of p16 and p18 expression in transduced MDSCs since the cyclin-dependent kinase inhibitors p16INK4A (p16) and p18INK4C (p18) cause early G1-phase cell cycle blockade. (pitt.edu)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Administration of specific BRAF V600E inhibitors resulted in decreased expression of MGL-binding glycans. (oncotarget.com)

To identify the prognostic value of CDKN2A (p16INK4a) and miRNAs involved in its regulation as markers of OSCC. (exp-oncology.com.ua)
miR-10b, -155, and CDKN2A mRNA expression in tumor samples was ana- lyzed by real-time reverse transcription polymerase chain reaction. (exp-oncology.com.ua)
No association of CDKN2A mRNA and p16INK4a protein with Ki-67 expression in tumor tissue and clinical pathological parameters of OSCC patients was found. (exp-oncology.com.ua)
Role of CDKN2A/p16 expression in the prognostication of oral squamous cell car- cinoma. (exp-oncology.com.ua)

This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
Each stage of the cell cycle is profiled by distinct protein complexes and phosphorylation events. (janechin.net)
In particular, hormonal activation of a conditional v-Jun-estrogen receptor fusion protein in quiescent, growth factor-deprived cells stimulates cyclin E-cdk2 activity and triggers Rb phosphorylation and DNA synthesis. (ox.ac.uk)

Mutations in p16INK4a were not found by SSCP analysis. (jax.org)
Point mutations introduced into the ATP catalytic domain failed to improve expression levels suggesting that protein function was not deleterious to the cell. (qmul.ac.uk)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Expression of p63 is almost exclusively restricted to epithelial cells, mutations in this gene are infrequent, and its expression is increased in a variety of solid tumors, particularly those of the head and neck area 12,13 . (bvsalud.org)
Signatures of somatic mutations and gene expression from p16INK4A positive head and neck squamous cell carcinomas (HNSCC). (cdc.gov)

The AML1-ETO is incapable of p14ARF transcription as the fusion protein took on AML1's involvement with ARF gene expression and ETO's transcription repression. (wikipedia.org)
The strongest multivariate model included: regional lymph node status, tumour grade, p16INK4A, cyclin D1 and p14ARF. (gsdermatology.com.au)
PAX3-FKHR, the catalytic unit of telomerase hTERT, and MycN, in cooperation with down-regulation of p16INK4A/p14ARF expression, were necessary and sufficient to convert normal human myoblasts into tumorigenic cells that gave rise to aRMS tumors. (duke.edu)
We therefore suggest that the translocation of PAX3 to FKHR drives proliferation of myoblasts, and a selection for loss of p16INK4A/p14ARF. (duke.edu)
Glioblastomas are morphological y and and loss at 9p21.1-24.3 (p16INK4a/ (until June 2010) genetical y heterogeneous, but little is p14ARF) , 10p15.3-q26.3 (PTEN, etc) and Dr Young Ho Kim known about the regional patterns of 13q12.11-q34 (SPRY2, RB1) . (who.int)

Chronic exposure to IL-1β induces only partial expression of senescence markers and does not allow us to conclude on its ability to induce senescence in chondrocytes. (aging-us.com)
On the other hand, etoposide treatment reliably induces DNA damage-related senescence in human articular chondrocytes evidenced by loss of proliferative capacity, DNA damage accumulation, and expression of some SASP components. (aging-us.com)
Initial observations indicate that CCN5 induces expression of P16INK4A and P19ARF, resulting in cell cycle arrest of the tumor cells. (ku.edu)

There were no significant associations between CDK2AP1 expression and estrogen receptor status, tumour grade and tumour type. (cancerindex.org)

Additionally, 38 patients with IgAN (19 aged over or equal to 60 years and 19 aged below 60 years) were randomly selected for p16INK4a staining by immunohistochemistry. (bvsalud.org)
Immunohistochemistry assay was performed in MAPK1 every Adriamycin inhibitor database the fifty eight examined situations, which thirty nine situations (67.2%) were positive for p16INK4A staining, 48 situations (82.8%) for EGFR and fifty one situations (87.9%) demonstrated positivity for p-EGFR. (antiviralbiologic.com)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)

Conclusions: In HNSCC, high XRCC1 protein expression is associated with poorer survival, particularly in patients receiving chemoradiation. (psu.edu)

However, one antibody recognised both ABCB5fl and ABCB5β, and was subsequently used to evaluate protein expression levels in other cell types.siRNA knockdown of ABCB5 in human mammary epithelial cells (HMECs) caused a concomitant reduction in p16 expression and an increase in cellular proliferation. (qmul.ac.uk)
Exogenous expression of each of five distinct putative oncomiRs in HMECs was found to increase cellular proliferation and, surprisingly, increase p16 expression. (qmul.ac.uk)
Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)
Expression of the INK4b-ARF-INKa locus during cellular ageing and differentiation . (biomedcentral.com)
The multidomain-containing cellular protein BS69 interacts with adenovirus E1A and several other viral and cellular factors and acts as a transcription repressor. (cancerrealitycheck.com)

Phosphorylated ETS1 and ETS2 stimulate p16INK4A transcription, resulting in cell cycle arrest with arrested cells exhibiting high p16INK4A level and senescence-associated beta-galactosidase activity. (reactome.org)
Crucial mediators Nesbuvir in BS69-shRNA-induced senescence At the moment two connected however specific senescence-associated pathways have already been determined: one concerning p53/p21Cip1 and the next concerning p16INK4a/Rb (Beausejour and in IMR90 cells in conjunction with knockdown. (cancerrealitycheck.com)

Western blot analysis confirmed the increased expression of cyclin D1 and cdk4 in lung tumors compared to normal lung. (jax.org)
These results suggest that alterations in the cell cycle proteins, cyclin D1, cdk4, p16INK4a, and Rb, may play a role in the acquisition of autonomous growth by adenomas. (jax.org)

Significance of expression of tumor-associated microrna-21 and- 375 for predicting the course of cancer of oral cavity. (exp-oncology.com.ua)
Furthermore, the expressions of the biomarkers didn't present any significant association with tumor levels. (antiviralbiologic.com)
During cancer progression, some tumor cells acquire new characteristics, such as over-expression of epithelial-mesenchymal transition (EMT) markers, and undergo profound morphogenetic changes. (biomedcentral.com)
The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)
The p63 protein, a homologue of p53, may be associated with tumor formation in the epithelial tissue, acting as an oncogene 11,12 . (bvsalud.org)
Mammary-specific expression of CCN5 in HER2 overexpressing mice delays tumor progression significantly and reduces the tumor burden. (ku.edu)
aRMS tissues exhibit a number of genetic changes, including loss-of-function of the p53 and Rb tumor suppressor pathways, amplification of MYCN, stabilization of telomeres, and most characteristically, reciprocal translocation of loci involving the PAX and FKHR genes, generating the PAX7-FKHR or PAX3-FKHR fusion proteins. (duke.edu)
The aim of the following study was to find difference the level for expression of the tumor specific gene p16 INK 4a in reactive, dysplastic and invasive changes in biopsy specimens from uterine cervix. (journal-imab-bg.org)
Elevated cell-surface expression of truncated carbohydrate structures such as Tn antigen (GalNAcα-Ser/Thr) is frequently observed during tumor progression. (oncotarget.com)

The oncoproteins E6 and E7 encoded with the risky HPV Adriamycin inhibitor database genotypes are well noted to play a crucial function in pathogenesis of anogenital carcinomas by deregulation of cell routine control proteins p53 and Adriamycin inhibitor database pRb2/p130, [12-18] respectively. (antiviralbiologic.com)

We found a strong correlation of the studied miRNAs expression levels with lymph node metastasis (r = 0.56 for miR-10b and r = 0.59 for miR-155). (exp-oncology.com.ua)
The association of both miRNAs with lymph node metastases was not affected by p16INK4a status. (exp-oncology.com.ua)

Few writers also have suggested involvement of risky HPVs in the oncogenesis through changed expression of various other key molecules involved with tyrosine kinase pathways, where inverse expressions of epidermal development aspect receptor (EGFR) and its own phosphorylated type (pEGFR) have already been reported to donate to the Adriamycin inhibitor database pathogenesis of HIV/AIDSCassociated SCCC, and correlates with poor prognosis [25-27]. (antiviralbiologic.com)
Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)

Expression of cell cycle proteins in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced mouse lung tumors. (jax.org)
In chemically induced mouse lung tumors, the p16INK4a gene is a target of inactivation, and Rb is reduced at the mRNA level (Northern blot) although similar at the protein level (Western blot) when compared to normal lung tissues. (jax.org)
Expression of p16INK4a, as determined by RT-PCR, was variable in lung tumors. (jax.org)
Expression of Rb was detected in normal lung tissues but was barely detectable by Northern blot hybridization in lung tumors. (jax.org)
Western blot analysis indicated the presence of both hypophosphorylated and hyperphosphorylated Rb protein in lung tumors and in normal lung tissues. (jax.org)
Prognostic significance of the expression of matrix metallopro- teinases 1 and 9 in tumors in patients with squamous cell cancer of the oral part of the pharynx. (exp-oncology.com.ua)
Conclusion: p63, p16, MIB, Cal A, Cys A are markedly expressed and p16 is strongly suppressed in oral cavity tumors, which suggests that the latter protein may play a role in negative regulation of cell cycle progression. (bvsalud.org)
As these tumors mostly lack CCN5 expression, we anticipate that restoration of CCN5 expression might be able to provide breakthroughs in the treatment of these tumors. (ku.edu)
However, the order of expression of these transgenes was critical, as only those cells expressing PAX3-FKHR early could form tumors. (duke.edu)

The up-regulation of proto-oncogene may be the consequence of epigenetic control of gene expression by demethylase, and mbd2 is involved in the regulation of hMSH2 expression in human gastric cancer. (wjgnet.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Another protein, calgranulin A (Cal A), is involved in the regulation of several cell processes, including the cell cycle and cell differentiation. (bvsalud.org)

Multivariable analysis revealed only HPV16 E6/E7 mRNA expression to have significant prognostic influence ( p = 0.04). (biomedcentral.com)
Larger studies are required to validate the frequency of HPV16 mRNA expression in HNSCC. (biomedcentral.com)
Gera R, Mokbel L, Jiang WG, Mokbel K mRNA Expression of Cancer Genomics Proteomics. (cancerindex.org)
Our objective was to determine if CDK2AP1 mRNA expression levels were consistent with tumour-suppressive functions in breast cancer. (cancerindex.org)
To our knowledge, this is the first study in the literature to examine the mRNA expression of CDK2AP1 in human breast cancer over a long-term follow-up period. (cancerindex.org)

p16INK4a expression positively and negatively predicted the presence of higher-grade lesions in 17.19% and 86.67% patients, respectively. (bvsalud.org)
The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. (cancerindex.org)
Consistently the levels of Bmi1 a member of the group transcription repressor which negatively regulates the expression of (Park messenger RNA levels were affected by BS69-shRNA we collected total RNA from adenovirus-infected IMR90 cells and carried out both semiquantitative and real-time reverse transcription-PCR (RT-PCR). (cancerrealitycheck.com)

this family of proteins is hereafter called EBF) are a group of DNA-binding transcription factors with the basic helix-loop-helix (bHLH) domain. (aacrjournals.org)
Our data indicated that this induction of p21Cip1 protein expression by BS69-shRNA was mainly due to enhanced transcription of gene. (cancerrealitycheck.com)
However, recent studies in many organisms and in humans have revealed significant protein sequence variation due to the presence of somatically acquired genetic variants, alternative transcription, and mRNA splicing, which are not necessarily annotated in reference databases. (biorxiv.org)
10 Similarly, in recent studies, at least one third of genes in diverse organisms can exhibit alternative transcription, leading to the production of N-terminally extended proteins or alternative reading frames. (biorxiv.org)

Conversely, expression of CaMKIV inhibits autophosphorylation and activation of CaMKII, and elicits G0/G1cell cycle arrest,impairing cell proliferation. (unisa.it)
Our data reveal a novel cross-talk between CaMKII and CaMKIV and suggest that CaMKII suppresses the expression of CaMKIV to promote leukemia cell proliferation. (unisa.it)
Effects of genistein and daidzein on hippocampus neuronal cell proliferation and BDNF expression in H19-7 neural cell line[J]. Journal of Nutrition Health & Aging 2012 16(4): 389-394. (paperonce.org)

Moreover, we show that the Polycomb protein BMI1 interacts with CDC6, an essential regulator of DNA replication in eukaryotic cells. (plos.org)
Our results suggest that in young cells Polycomb proteins are recruited to the INK4/ARF locus through CDC6 and the resulting silent locus is replicated during late S-phase. (plos.org)
Upon senescence, Jmjd3 is overexpressed and the MLL1 protein is recruited to the locus provoking the dissociation of Polycomb from the INK4/ARF locus, its transcriptional activation and its replication during early S-phase. (plos.org)

We found that mammary epithelium-specific overexpression of CCN5 in transgenic mice leads to an increase in ER-α expression and that this impact of CCN5 is not restricted to the normal cells. (ku.edu)
Finally, we discuss the effects of CCN5 expression on yet another aggressive breast cancer subtype, characterized by HER2 overexpression. (ku.edu)

To explore the effect of DNA methyltransferase, demethylase and methyl-CpG binding protein MeCP2 on the expressions and methylation of hMSH2 and proto-oncogene in human gastric cancer. (wjgnet.com)

2012). The NUP214 protein is involved in mRNA export as well as nuclear membrane localization and nuclear pore complex (Koser et al. (wikipedia.org)
The expressions of p53 p21 kelch-like epichlorohydrin-associated protein-1 (Keap1) nuclear factor E2-associated factor (Nrf2) heme oxygenase-1(HO-1) proteins in H9c2 cells were detected with the western blotting method. (paperonce.org)

Another cell routine regulatory proteins whose increased appearance is reported to become predictive of, and an unbiased prognostic marker in risky HPV infections is p16INK4A[19-22]. (antiviralbiologic.com)
Here we aimed to evaluate whether MGL ligand expression has prognostic value and whether this was correlated to BRAF V600E mutation status. (oncotarget.com)

DEK functions as a transcriptional repressor by interfering with histone acetyl transferases, regulator for a number of stem cells, and activates gene expression in myeloid cells (Koleva et al. (wikipedia.org)
p16INK4a/Ki-67 co-expression specifically identifies transformed cells in the head and neck region. (exp-oncology.com.ua)
Exogenous expression of ABCB5fl and ABCB5β was achieved in HEK293T cells, but the recombinant protein expressed poorly and localised to the endoplasmic reticulum. (qmul.ac.uk)
Exogenous expression in HEK293T cells also allowed commercial antibodies purportedly raised against ABCB5 isoforms to be tested. (qmul.ac.uk)
The percentage of p16INK4a-positive cells in glomeruli, renal tubule and interstitium were separately quantified. (bvsalud.org)
The percentage of glomerular and tubular p16INK4a-positive cells in elderly patients showed an increasing trend, but statistical significance was not reached. (bvsalud.org)
Next, we have shown that CCN5 protein can induce expression of estrogen receptor-α (ER-α) in mammary epithelial cells. (ku.edu)
CCN5 treatment leads to an expression of functional ER-α in the TNBC cells, both in-vitro and in xenograft models, and sensitizes these cells to tamoxifen, commonly used for endocrine therapies. (ku.edu)
Lack of ER-α expression in TNBC cells or loss of ER-α activation after endocrine treatment of luminal cancers makes these breast cancer cells resistant to tamoxifen and other endocrine therapies. (ku.edu)
Bone morphogenetic protein 4 (BMP4) promotes the osteogenic potential & the bone regenerative potential of muscle-derived stem cells (MDSCs). (pitt.edu)
We previously showed that PAX3-FKHR expression in primary human myoblasts, cells that can give rise to RMS, cooperated with loss of p16INK4A to promote extended proliferation. (duke.edu)
Indeed we detected induction of p21Cip1 but not p53 or p16INK4a in IMR90 cells infected with the lentivirus expressing BS69-shRNA (Fig 1D). (cancerrealitycheck.com)
The results showed that DAI decreased the positive staining of SA- -gal down-regulated the expression of p53 and p21 proteins increased the T-AOC level and SOD activity decreased the MDA and ROS levels and maintained the stability of mitochondrial membrane potential in aging H9c2 cells induced by H2O2. (paperonce.org)

Cyclin D1 dysregulation and differential inactivation of p16INK4a and Rb have been observed in human lung cancer. (jax.org)
In agreement with differential derepression, PcG protein binding across the locus is discontinuous. (biomedcentral.com)

Results: The expression of p16 protein was not associated with the severity of epithelial dysplasia (81.5% in low-grade and 59.2% in high-grade cases) (P=0.16). (ac.ir)

8. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. (journal-imab-bg.org)

Protein expression of the pRb and p53 pathways and related molecules that directly or indirectly influence cell cycle progression at the G1/S phase checkpoint was assessed. (gsdermatology.com.au)

METHODS: In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. (bvsalud.org)

OBJECTIVES: To determine the ability of p16INK4a and Ki-67 staining in biopsies diagnosed with CIN2 to identify patients with higher-grade lesions (CIN3 or carcinoma). (bvsalud.org)
In the lesions of the uterine cervix, over expression of p16 INK 4a is induced by HPV and is associated with the process of cancerogenesis in the epithelium. (journal-imab-bg.org)
Almost 100% of the high grade lesions and the invasive carcinomas demonstrated high level of expression of p16 INK 4a while non dysplastic epithelium is always negative. (journal-imab-bg.org)

The detection of HPV DNA alone, in the absence of evidence for viral gene expression, is not unequivocal molecular evidence that HPV infection either causes or promotes malignant progression in a lesion. (biomedcentral.com)
Studies suggest that expression of cystatin A is inversely associated with malignant progression of cancer 9 . (bvsalud.org)

The expression of these two biomarkers was verified by at least two independent pathologists and quantified using digital algorithms. (bvsalud.org)

The percentage of p16INK4a-positive nuclei in renal interstitium was positively associated with T score, while increased percentage of p16INK4a-positive nuclei in renal tubule was associated with eGFR and 24-h urinary protein level. (bvsalud.org)
Significant statistical association was discovered between HPV recognition and p16INK4 (p=0.000, at 99% C.We) and EGFR (p=0.028, in 95% C.We) expressions, however, not pEGFR. (antiviralbiologic.com)
Conclusion This research points to a link of risky HPV with over expressions of p16INK4A and EGFR proteins in AIDS-associated SCCC. (antiviralbiologic.com)

Positive IHC staining for p16INK4a and Ki-67 did not predict CIN3 in patients with CIN2 biopsies. (bvsalud.org)

One of the hallmarks of M2 subtype acute myeloid leukemia is the formation of a fusion protein, AML1-ETO or RUNX1-RUNX1T1, due to a translocation of chromosome 8 to chromosome 21 or t(8;21) (Miyoshi et al. (wikipedia.org)
The fusion protein AML1-ETO is commonly found in acute myeloid leukemia patients. (wikipedia.org)

On both sides of the splice the DNA coded for different proteins, RUNX1 and ETO, These two sequences are then transcribed and translated into a single large protein, "M2 AML" which allows the cell to divide unchecked, leading to cancer. (wikipedia.org)
Normalised CDK2AP1 expression was 38-fold higher in adjacent non-cancerous breast tissue than in breast cancer. (cancerindex.org)
CDK2AP1 expression in disease-free patients at 10 years was more than threefold that of patients who died of breast cancer. (cancerindex.org)
11 And human diseases, including cancer in particular, tend to be defined by the presence of proteins with altered and pathogenic sequences. (biorxiv.org)

This study investigates the modulation of p16 function by the putative membrane protein ABCB5 and a group of five putative oncogenic microRNAs (oncomiRs). (qmul.ac.uk)

Some new promising strategies for biomarker discovery include microarray-based profiling at the DNA and mRNA levels, and mass-spectrometry-based profiling at the protein and peptide levels [ 2 ]. (biomedcentral.com)
However, most proteomic studies rely on consensus databases to match spectra to peptide and proteins sequences, and thus remain limited to the analysis of canonical protein sequences. (biorxiv.org)
Most commonly, this approach leverages high-resolution mass spectrometric measurements of proteolyzed proteins, combined with tandem peptide fragmentation to match observed spectra with those expected from their amino acid composition. (biorxiv.org)
Current approaches for peptide and protein identification use advanced statistical and graphical methods to match mass spectra and estimate their confidence. (biorxiv.org)

For example, most human tissues in healthy individuals acquire somatic nucleotide substitutions, insertions, deletions and DNA rearrangements, leading to the production of variant protein isoforms. (biorxiv.org)

Anatomy26

Organisms9

Diseases23

Chemicals and Drugs93

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Psychiatry and Psychology1

Phenomena and Processes62

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care2

Ink4a19

Squamous cell carc5

Retinoblastoma5

Immunohistochemical7

Examined by immunohistochemistry1

Senescent cells2

Inhibitors5

Cdkn2a4

Phosphorylation4

Mutations5

P14ARF5

Induces3

Tumour grade1

Immunohistochemistry3

Conclusions1

Cellular5

Senescence-associated2

Cdk42

Tumor9

P1301

Lymph node2

Inhibitor2

Tumors9

Regulation3

MRNA expression5

Negatively3

Transcription4

Cell Proliferation3

Polycomb3

Overexpression2

Methylation1

Nuclear2

Prognostic2

Cells13

Differential2

Epithelial1

Cervical1

Pathways1

Correlation1

Lesions3

Progression2

Biomarkers1

EGFR3

CIN21

Fusion2

Cancer4

Modulation of p161

Peptide4

Isoforms1