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InkTumor Suppressor Protein p14ARFCyclin-Dependent Kinase Inhibitor p16ADP-Ribosylation FactorsADP-Ribosylation Factor 1Cyclin-Dependent Kinase Inhibitor p15Cyclin-Dependent Kinase Inhibitor p18Cyclin-Dependent Kinase Inhibitor p19Genes, p16Cell AgingCyclin-Dependent Kinase 4Tumor Suppressor Protein p53GTPase-Activating ProteinsTumor Suppressor ProteinsChromosomes, Human, Pair 9Retinoblastoma ProteinCarrier ProteinsCyclin-Dependent KinasesCell Cycle ProteinsProto-Oncogene Proteins c-mdm2DNA MethylationAcute Kidney InjuryGenes, Tumor SuppressorCyclin-Dependent Kinase 6Gene DeletionPolycomb Repressive Complex 1Brefeldin AGuanine Nucleotide Exchange FactorsProto-Oncogene ProteinsCell CycleCoatomer ProteinMutationCoat Protein Complex IGenes, p53Cyclin-Dependent Kinase Inhibitor p21Golgi ApparatusNuclear ProteinsPhospholipase DMolecular Sequence DataGuanosine TriphosphateCell Transformation, NeoplasticGene SilencingFibroblastsPromoter Regions, GeneticGTP-Binding ProteinsCyclinsGene Expression Regulation, NeoplasticPolymorphism, Single-Stranded ConformationalPrintingProtein BindingDNA, NeoplasmPolymerase Chain ReactionProteinsImmunohistochemistryCell LineCell DivisionGenes, rasSignal TransductionTumor Cells, CulturedInk Blot TestsG1 PhaseCyclin-Dependent Kinase Inhibitor p27Guanosine DiphosphateCells, CulturedCyclin D1E2F1 Transcription FactorLoss of HeterozygosityTattooingCpG IslandsBase SequenceProtein TransportRepressor ProteinsCell Line, TumorCell ProliferationHeLa CellsRNA, MessengerGenes, RetinoblastomaAmino Acid SequenceMice, KnockoutTransfectionGene Expression RegulationDNA-Binding ProteinsPhosphatidylinositol 4,5-DiphosphateEndosomesGuanosine 5'-O-(3-Thiotriphosphate)ExonsMice, TransgenicReverse Transcriptase Polymerase Chain ReactionTranscription FactorsRheumatic FeverMice, Inbred C57BLCell Nucleolustrans-Golgi NetworkCervical Intraepithelial NeoplasiaApoptosisMelanomaCyclin DPolycomb-Group ProteinsRNA, Small InterferingReading Frames
P19ARFCDKN2AINK4aMdm2Tumor suppressorApoptosisProteinExpressionCyclinProliferationHumanCellIndependentP15INK4bGenesGeneTranscriptExpressionCell cycleMouse
P19ARF3
  • In humans, ARF is translated into the 14kDa, 132 amino acid [[p14ARF]] protein, and in mice, it is translated into the 19kDa, 169 amino acid p19Arf. (wikipedia.org)
  • UniProt P42771-3), p14ARF/p19ARF or smARF (UniProt Q8N726-1 and Q8N726-2). (sigmaaldrich.com)
  • P16INK4A and P19ARF (P14ARF in humans) are two tumor suppressor genes encoded by INK4a-ARF and inhibited by Bmi-1. (docksci.com)
CDKN2A4
  • p14ARF (also called ARF tumor suppressor, ARF, p14ARF) is an alternate reading frame protein product of the CDKN2A locus (i.e. (wikipedia.org)
  • Loss of p14ARF by a homozygous mutation in the CDKN2A (INK4A) gene will lead to elevated levels in mdm2 and, therefore, loss of p53 function and cell cycle control. (wikipedia.org)
  • Simultaneous disturbance of these pathways can occur via co-amplification of MDM2 and CDK4 or homozygous deletion of CDKN2A, which encodes both p14ARF and p16INK4a. (uu.nl)
  • also known as CDK4I, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, MTS-1, p16-INK4, p16-INK4a, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID 1029) in human. (sigmaaldrich.com)
INK4a3
  • It is located near the genes for the tandem repeats INK4a and INK4b, which are 16 kDa (p16INK4a) and 15 kDa (p15INK4b) proteins, respectively. (wikipedia.org)
  • INK4a and p14ARF mRNA each consist of three exons. (wikipedia.org)
  • Because of this, INK4a and p14ARF have unrelated amino acid sequences despite overlapping coding regions and have distinct functions. (wikipedia.org)
Mdm22
  • p14ARF inhibits mdm2, thus promoting p53, which promotes p21 activation, which then binds and inactivates certain cyclin-CDK complexes, which would otherwise promote transcription of genes that would carry the cell through the G1/S checkpoint of the cell cycle. (wikipedia.org)
  • In contrast with adult cancers, p53 pathway inactivation in neuroblastomas is rarely caused by p53 mutation but rather by altered MDM2 or p14ARF expression. (uu.nl)
Tumor suppressor1
  • These interactions allow p14ARF to act as a tumor suppressor by inhibiting ribosome biogenesis or initiating p53-dependent cell cycle arrest and apoptosis, respectively. (wikipedia.org)
Apoptosis1
  • Telomerase is excessively activated in most of tumors and is involved in the regulation of cell cycle proteins, such as p16Ink4a and p21WAF1, which induce epithelial cell apoptosis [7]. (docksci.com)
Protein5
  • p14ARF is induced in response to elevated mitogenic stimulation, such as aberrant growth signaling from MYC and Ras (protein). (wikipedia.org)
  • p14ARF is an atypical protein, in terms of its transcription, its amino acid composition, and its degradation: it is transcribed in an alternate reading frame of a different protein, it is highly basic, and it is polyubiquinated at the N-terminus. (wikipedia.org)
  • The p14ARF transcript was first identified in humans in 1995, and its protein product confirmed in mice that same year. (wikipedia.org)
  • This dual-use of coding sequences is not commonly seen in mammals, making p14ARF an unusual protein. (wikipedia.org)
  • NPM is an acidic ribosomal chaperone (protein) involved in preribosomal processing and nuclear exportation independent of p53, and oligomerizes with itself and p14ARF. (wikipedia.org)
Expression3
  • rates, frequently triggered by pRb pathway dysfunction due to aberrant expression of cyclin D1, CDK4 or p16INK4a. (uu.nl)
  • We previously showed that PAX3-FKHR expression in primary human myoblasts, cells that can give rise to RMS, cooperated with loss of p16INK4A to promote extended proliferation. (duke.edu)
  • PAX3-FKHR, the catalytic unit of telomerase hTERT, and MycN, in cooperation with down-regulation of p16INK4A/p14ARF expression, were necessary and sufficient to convert normal human myoblasts into tumorigenic cells that gave rise to aRMS tumors. (duke.edu)
Cyclin2
  • The strongest multivariate model included: regional lymph node status, tumour grade, p16INK4A, cyclin D1 and p14ARF. (gsdermatology.com.au)
  • Specifically the molecular data identifies novel markers of outcome in SCC of the anterior tongue such as p14ARF, and therapeutic response to ZD1839 such as cyclin D1 and E2F-1. (gsdermatology.com.au)
Proliferation2
  • We therefore suggest that the translocation of PAX3 to FKHR drives proliferation of myoblasts, and a selection for loss of p16INK4A/p14ARF. (duke.edu)
  • P16INK4a can directly inhibit cell proliferation by inhibiting Rb phosphorylation and preventing cells from entering S phase (DNA synthesis phase). (docksci.com)
Human2
  • GST-tagged recombinant human p16INK4a internal fragment. (sigmaaldrich.com)
  • Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections. (sigmaaldrich.com)
Cell2
  • Both p16INK4a and p14ARF are involved in cell cycle regulation. (wikipedia.org)
  • Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate. (sigmaaldrich.com)
Independent1
  • This is the first study to determine that p14ARF is an independent marker of both improved disease free survival and overall survival in a cohort of SCC of the anterior tongue. (gsdermatology.com.au)
P15INK4b1
  • Cloning and characterization of murine p16INK4a and p15INK4b genes. (google.com.sg)
Genes4
  • This study evaluated the cell cycle regulatory genes, p16INK4a and p14ARF, for homozygous deletion, loss of heterozygosity, and mutation events in 20 PVL cases. (nih.gov)
  • p14ARF inhibits mdm2, thus promoting p53, which promotes p21 activation, which then binds and inactivates certain cyclin-CDK complexes, which would otherwise promote transcription of genes that would carry the cell through the G1/S checkpoint of the cell cycle. (wikipedia.org)
  • This protein constitutes a complex with HDAC1 also via its SRA domain, and bound to methylated promoter regions of various tumor suppressor genes, including p16INK4A and p14ARF, in cancer cells. (elsevierpure.com)
  • Genes supresores de tumores localizados en el cromosoma 9 humano en la región 9p21. (bvsalud.org)
Gene2
  • We tested urine sediment DNA for aberrant methylation of nine gene promoters (p16INK4a, p14(ARF), MGMT, GSTP1, RARbeta2, CDH1 [E-cadherin], TIMP3, Rassf1A, and APC) from 52 patients with prostate cancer and 21 matched primary tumors by quantitative fluorogenic real-time polymerase chain reaction. (nih.gov)
  • It is ubiquitously expressed in many tissues and cell types.The gene codes for two proteins, including the INK4 family member p16 (or p16INK4a) and p14arf. (microbiologystudents.com)
Transcript1
  • The p14ARF transcript was first identified in humans in 1995, and its protein product confirmed in mice that same year. (wikipedia.org)
Expression2
  • METHODS: In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. (bvsalud.org)
  • p16INK4a expression positively and negatively predicted the presence of higher-grade lesions in 17.19% and 86.67% patients, respectively. (bvsalud.org)
Cell cycle1
Mouse2
  • Human IgG antibody Laboratories manufactures the mouse monoclonal anti-p16ink4a antibody reagents distributed by Genprice. (microbiologystudents.com)
  • The Mouse Monoclonal Anti-P16Ink4A Antibody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (microbiologystudents.com)