Bacteriophage P1GenomeProteinLytic phageProteinsGenusGenomesColiGenomicVirusesLambdaParticlesCaudoviralesGeneraLysisCharacterizationSequenceContigsIsolatesTaxonomicBacteriophagesVitroGenesBacterialIdentificationPossessInfectSequencesAntitoxinSpeciesStrainStructureSeparateTherapyVirusGroupsTotalPopulationStudy
Bacteriophage P12
- The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1. (wikipedia.org)
- Our crystal structures of Phd-Doc from bacteriophage P1, the HigA antitoxin from Escherichia coli CFT073, and YeeU of the YeeUWV systems from E. coli K12 and Shigella flexneri confirm this inference and reveal additional, unanticipated structural relationships. (nih.gov)
Genome4
- Here, we reported the first Oceanospirillum phage, vB_OliS_GJ44, which was assembled into a 33,786 bp linear dsDNA genome, which includes abundant tail-related and recombinant proteins. (biomedcentral.com)
- Tailed dsDNA phages encapsidate their genome as a linear molecule, but depending on the terminal genomic arrangement, many complete phage genomes assemble into a "circular" contig (i.e., a contig with direct terminal repeats) [ 12 ]. (biomedcentral.com)
- STED, stimulated emission depletion IntroductionRibonucleoprotein (RNP) granules are a conserved mechanism to alter granule function is promoted by decapping activators that recruit DCP1 to the WT lambda phage genome mutation. (billfryer.com)
- The complete genome of the phage Βϕ-R2096, which belongs to the Myoviridae family, was analyzed. (biomedcentral.com)
Protein4
- The "Gratiaviridae" phages encode a HipA-family protein kinase and glycosyltransferase, suggesting these phages modify the host cell wall, preventing superinfection by other phages. (biomedcentral.com)
- In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. (ophrp.org)
- Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. (embl.de)
- This entry represents a subtype of the immunoglobulin domain, and is found in a diverse range of protein families that includes glycoproteins, fibroblast growth factor receptors, vascular endothelial growth factor receptors, interleukin-6 receptor, and neural cell adhesion molecules. (embl.de)
Lytic phage3
- They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. (ophrp.org)
- In this study, a novel A. baumannii lytic phage, the YMC 13/03/R2096 ABA BP (phage Βϕ-R2096), which specifically causes the lysis of CRAB strains, was characterized in detail in vitro and in silico, and the in vivo effectiveness of phage therapy was evaluated using Galleria mellonella and a mouse model of acute pneumonia. (biomedcentral.com)
- The results of this study strongly suggest that phage Βϕ-R2096, a novel A. baumannii lytic phage, could be an alternative antibacterial agent to control CRAB infections. (biomedcentral.com)
Proteins4
- In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
- PARP-1 is the best-characterized member of the diphtheria toxin-like ADP-ribosyl transferases (ARTDs) family of proteins. (nature.com)
- Doc (death on curing) proteins from phage P1 and several bacteria. (expasy.org)
- is usually multifactorial, involving the coordinate action of main adhesin molecules (P1 and P30) and several high-molecular-weight accessory membrane proteins that act in concert with cytoskeletal elements to facilitate the lateral movement and concentration of the adhesin buy Kaempferol-3-rutinoside molecules in the attachment organelle (4, 9, 15, 16, 29, 32, 38, 39). (careersfromscience.org)
Genus5
- A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE , which infects E. coli. (nih.gov)
- Genomic and morphological analyses identified vB_OliS_GJ44 as a siphovirus, however, due to the distant evolutionary relationship with any other known siphovirus, it is proposed that this virus could be classified as the type phage of a new Oceanospirivirus genus within the Siphoviridae family. (biomedcentral.com)
- Homologs of several vB_OliS_GJ44 genes have mostly been found in marine metagenomes, suggesting the prevalence of this phage genus in the oceans. (biomedcentral.com)
- Thus, we propose a new viral genus Oceanospirivirus within the Siphoviridae family to reconcile this cluster, with vB_OliS_GJ44 as a representative member. (biomedcentral.com)
- Oceanospirillum is the type genus of the family Oceanospirillaceae , in the order Oceanospirillales of the class Gammaproteobacteria . (biomedcentral.com)
Genomes5
- A search of human gut metagenomes for circular contigs encoding phage hallmark genes resulted in the identification of 3738 apparently complete phage genomes that represent 451 putative genera. (biomedcentral.com)
- Comparative genomic analysis of the three candidate phage families revealed features without precedent in phage genomes. (biomedcentral.com)
- Analysis of phage genomes identified in whole-community human gut metagenomes resulted in the delineation of at least three new candidate families of Caudovirales and revealed diverse putative mechanisms underlying phage-host interactions in the human gut. (biomedcentral.com)
- The rapid growth of the public whole-community metagenomic data offers the opportunity to identify numerous novel phage genomes lurking in metagenomes. (biomedcentral.com)
- Thus, circularity can be used as one feature to identify putative complete phage genomes in viromes and metagenomes. (biomedcentral.com)
Coli2
- Isolation and characterization of a new generalized transducing bacteriophage different from P1 in Escherichia coli . (ulaval.ca)
- P1 transduction map spanning the replication terminus of Escherichia coli K12. (microbiologyresearch.org)
Genomic1
- Although the human gut offers a rich source of phage genomic diversity, the virome so far has been explored to a much lesser extent than the whole community (metagenome), composed of viruses, bacteria, and archaea. (biomedcentral.com)
Viruses1
- A multilevel framework for taxonomic classification of viruses was recently adopted, facilitating the classification of phages into evolutionary informative taxonomic units based on hallmark genes. (biomedcentral.com)
Lambda2
- Viable phage particles were produced by in vitro packaging of recombinant phage DNA using a commercial in vitro lambda DNA packaging system (Promega). (careersfromscience.org)
- op or pr) fusion gene was each crossed into lambda phage. (openmicrobiologyjournal.com)
Particles1
- Single-mutant phages were used for the analysis of DCP1 overexpression in the Supporting information figures contain replicates that were exposed to enough phage particles (Fig 5B). (billfryer.com)
Caudovirales1
- Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the Siphoviridae family and the Caudovirales order. (ophrp.org)
Genera1
- Several of these phage genera are only distantly related to previously identified phages and are likely to found new families. (biomedcentral.com)
Lysis1
- We observed a strong promoter, causing eventual lysis of cultures infected with phage diluted 1:8. (billfryer.com)
Characterization1
- Addition of these phylogenetically classified, diverse, and distinct phages to public databases will facilitate taxonomic decomposition and functional characterization of human gut viromes. (biomedcentral.com)
Sequence2
- Together with advanced approaches for sequence assembly and powerful methods of sequence analysis, this revised framework offers the opportunity to discover and classify unknown phage taxa in the human gut. (biomedcentral.com)
- Sequence analyses suggest that TA families are linked by complex evolutionary relationships reflecting likely swapping of functional domains between different TA families. (nih.gov)
Contigs1
- vB_OliS_GJ44 showed synteny with six uncultured phages, which supports its representation in uncultured environmental viral contigs from metagenomics. (biomedcentral.com)
Isolates2
- Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. (mdpi.com)
- In the evaluation of its therapeutic potential against CRAB clinical isolates using two in vivo models, phage Βϕ-R2096 increased the survival rates of both G. mellonella larvae (from 0 to 50% at 24 h) and mice (from 30% with MOI = 0.1 to 100% with MOI = 10 for 12 days) in post-infection of CRAB. (biomedcentral.com)
Taxonomic1
- Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. (ophrp.org)
Bacteriophages1
- The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. (ophrp.org)
Vitro1
- These in vitro cleavage and phage with preexisting mutations may drive further mutation in the target. (billfryer.com)
Genes1
- Some "Quimbyviridae" phages possess Diversity-Generating Retroelements (DGRs) that generate hypervariable target genes nested within defense-related genes, whereas the previously known targets of phage-encoded DGRs are structural genes. (biomedcentral.com)
Bacterial2
- Phage Βϕ-R2096 inhibited bacterial growth in a dose-dependent manner and exhibited high bacteriolytic activity at MOI = 10. (biomedcentral.com)
- In particular, phage Βϕ-R2096 strongly ameliorated histologic damage to infected lungs, with bacterial clearance in the lungs observed on day 3 postinfection in the mouse acute pneumonia model. (biomedcentral.com)
Identification1
- Thus, an increase in phage identification will promote a better understanding of their evolution and their effects on microbial communities and biogeochemical cycles. (biomedcentral.com)
Possess1
- These findings suggest that these pathogenic mycoplasmas possess a family of conserved cytadhesin molecules used to colonize widely divergent hosts. (careersfromscience.org)
Infect2
- Two of the candidate families, "Flandersviridae" and "Quimbyviridae", include some of the most common and abundant members of the human gut virome that infect Bacteroides , Parabacteroides , and Prevotella . (biomedcentral.com)
- Analysis of CRISPR spacers indicates that phages of all three putative families infect bacteria of the phylum Bacteroidetes. (biomedcentral.com)
Sequences1
- effective sequences of family are to separate microbial data. (naturheilpraxis-gisbert-fussek.de)
Antitoxin1
- Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation. (expasy.org)
Species1
- Hundreds of phages in these three and other families are shown to encode catalases and iron-sequestering enzymes that can be predicted to enhance cellular tolerance to reactive oxygen species. (biomedcentral.com)
Strain1
- A recombinant phage library was constructed in the phage vector gt11 (Promega) using partially digested strain R expressing the 55-kDa product of PvpA (49). (careersfromscience.org)
Structure2
- The present invention is relative to chimerical peptides, whose primary structure holds at least one segment which inhibits the activation of the NS3 protease of a virus from the Flaviviridae family, they also contain a cell penetrating segment and they are capable of inhibiting or attenuate the viral infection. (patentsencyclopedia.com)
- 1. Chimerical peptides, characterized by having a primary structure comprising at least one segment inhibitor of the activation of the NS3pro protease from a virus of the Flaviviridae family and a cell penetrating segment and these peptides are able to inhibit or attenuate the infection by the virus. (patentsencyclopedia.com)
Separate1
- JM are a stable free janes battles with the luftwaffe the bomber campaign against germany 1942 1945 2001 for bending successful CIRS of instructive repressors which is separately continuous in Separate transformants for undergraduate tij− and phage variable. (scoutconnection.com)
Therapy1
- In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells. (ophrp.org)
Virus2
- This invention is also relative to pharmaceutical compounds which contain these chimerical peptides for the prevention and/or treatment of the infection caused by a virus of the Flaviviridae family. (patentsencyclopedia.com)
- 10. Chimerical peptides according to the claim 1, where the mentioned virus from the Flaviviridae family is a Hepacivirus and specifically HCV. (patentsencyclopedia.com)
Groups2
- yet, the gut virome is far from being fully characterized, and additional groups of phages, including highly abundant ones, continue to be discovered by metagenome mining. (biomedcentral.com)
- Moreover, in vivo studies revealed no mortality or serious side effects in phage-treated groups. (biomedcentral.com)
Total1
- En el cuadro 1 se presenta un anlisis de los genocentros de las plagas agrcolas, no necesariamente primarias ni secundarias, y algunas cuarentenarias ms relevantes para Chile con un total de especies, informacin de la cual se concluye: a El Orden Coleoptera es el que ms contribuye 2. (kaltner-autohandel.at)
Population1
- The phage population present in liquid culture to a 1. (billfryer.com)
Study1
- This study is the first report to compare in vivo evaluations ( G. mellonella larvae and a mouse acute pneumonia model) of the therapeutic efficacy of a phage against CRAB infections. (biomedcentral.com)